• Journal of The Korean Society of Inherited Metabolic disease
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• v.3 no.1
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• pp.86-93
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• 2003

Background : The SCL began screening of newborns and high risk group blood spots with tandem mass spectrometry (MS/MS) in April 2001. Our goal was to determine approximate prevalence of metabolic disorders, optimization of decision criteria for estimation of preventive effect with early diagnosis. This report describes the ongoing effort to identify more than 30 metabolic disorders by MS/MS in South Korea. Methods : Blood spot was collected from day 2 to 30 (mostly from day 2 to 10) after birth for newborn. Blood spot of high risk group was from the pediatric patients in NICU, developmental delay, mental retardation, strong family history of metabolic disorders. One punch (3.2 mm ID) of dried blood spots was extracted with $150{\mu}L$ of methanol containing isotopically labelled amino acids (AA) and acylcarnitines (AC) internal standards. Butanolic HCl was added and incubated at $65^{\circ}C$ for 15 min. The butylated extract was introduced into the inlet of MS/MS. Neutral loss of m/z 102 and parent ion mode of m/z 85 were set for the analyses of AA and AC, respectively. Diagnosis was confirmed by repeating acylcarnitine profile, urine organic acid and plasma amino acid analysis, direct enzyme assay, or molecular testing. Results : Approximately 31,000 neonates and children were screened and the estimated prevalence (newborn/high risk group), sensitivity, specificity and recall rate amounted to 1:2384/1:2066, 96.55%, 99.98%, and 0.73%, respectively. Confirmed 28 (0.09%) multiple metabolic disorders (newborn/high risk) were as follows; 13 amino acid disorders [classical PKU (3/4), BH4 deficient-hyperphenylalaninemia (0/1), Citrullinemia (1/0), Homocystinuria (0/2), Hypermethioninemia (0/1), Tyrosinemia (1/0)], 8 organic acidurias [Propionic aciduria (2/1), Methylmalonic aciduria (0/1), Isovaleric aciduria (1/1), 3-methylcrotonylglycineuria (1/0), Glutaric aciduria type1 (1/0)], 7 fatty acid oxidation disorders [LCHAD def. (2/2), Mitochondrial TFP def. (0/1), VLCAD def. (1/0), LC3KT def. (0/1). Conclnsion : The relatively normal development of 10 patients with metabolic disorders among newborns (except for the expired) demonstrates the usefulness of newborn screening by MS/MS for early diagnosis and medical intervention. However, close coordination between the MS/MS screening laboratory and the metabolic clinic/biochmical geneticists is needed to determine proper decision of screening parameters, confirmation diagnosis, follow-up scheme and additional tests.

• Journal of Plant Biotechnology
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• v.45 no.4
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• pp.369-374
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• 2018

The purpose of this study was to investigate the suitable parts for callus induction and optimal concentrations of growth regulators contained in the medium affecting shoot and rooting for the in vitro mass production of Haworthia maughanii. To determine suitable parts of the plant for callus induction, the leaves, flower bloom and flower stalks were cultured in MS medium at different concentrations of $0{\sim}2mgL^{-1}$ NAA and $0{\sim}2mgL^{-1}$ TDZ, respectively. All of the parts showed 100% callus formation rate at $NAA\;1mgL^{-1}$ and $TDZ\;1mgL^{-1}$ treatment, $NAA\;2mgL^{-1}$ and $TDZ\;2mgL^{-1}$ treatment and NAA 1 to $2mgL^{-1}$, respectively. While the rate of callus formation was high in all parts, the leaves were the most efficient to obtain most culture parts. $NAA\;0.1mg\;L^{-1}$ and $BA\;0.1mg\;L^{-1}$ treatments were the most effective in shoot formation with 22.0 shoots. In addition, multiple shoot propagation showed 16.3 shoots, the highest, with $NAA\;0.1mg\;L^{-1}$ and $BA\;0.1mg\;L^{-1}$ treatments. These results led us to speculate that the optimization of culture conditions was responsible for the mass propagation for in vitro cultures of Haworthia maughanii.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.32 no.1
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• pp.17-27
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• 2019

In this paper, we study the applicability of Tuned Mass Damper(TMD) to improve seismic performance of piping system under earthquake loading. For this purpose, a mode analysis of the target pipeline is performed, and TMD installation locations are selected as important modes with relatively large mass participation ratio in each direction. In order to design the TMD at selected positions, each corresponding mode is replaced with a SDOF damped model, and accordingly the corresponding pipeline is converted into a 2-DOF system by considering the TMD as a SDOF damped model. Then, optimal design values of the TMD, which can minimize the dynamic amplification factor of the transformed 2-DOF system, are derived through GA optimization method. The proposed TMD design values are applied to the pipeline numerical model to analyze seismic performance with and without TMD installation. As a result of numerical analyses, it is confirmed that the directional acceleration responses, the maximum normal stresses and directional reaction forces of the pipeline system are reduced, quite a lot. The results of this study are expected to be used as basic information with respect to the improvement of the seismic performance of the piping system in the future.

• Microbiology and Biotechnology Letters
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• v.48 no.3
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• pp.344-357
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• 2020

Strain improvement and bioprocess development were undertaken to enhance hyaluronic acid(HA) production by Streptococcus zooepidemicus cells. Using a high-yielding mutant strain, statistical medium optimization was carried out in shake flask cultures, resulting in 52% increase in HA production (5.38 g/l) at the optimal medium composition relative to the parallel control cultures. For sufficient supply of dissolved oxygen (DO), which turned out to be crucial for enhanced production of HA, agitation system and speed were intensively investigated in 5 L bioreactor cultures. Increase in oxygen mass transfer coefficient (k_La) through increment of agitation speed (rpm) and 35% expansion of diameter of the newly-designed impellers showed significantly positive effects on HA production. By installing an expanded Rushton-turbine impeller for efficient break-down of sparged air, and an extended marine impeller above the Rushton-turbine impeller for efficient mixing of the air-born viscous fermentation broth, maximum amount of HA (9.79 g/l) was obtained at 450 rpm, 1.8 times higher level than that of the corresponding flask culture. Subsequently, the possibility of bioprocess scale-up to a 50 L bioreactor was investigated. Despite almost identical maximum HA production (9.11 vs 9.25 g/l), the average HA volumetric productivity (r_p) of the 50 L culture turned out only 74% compared to the corresponding 5 L culture during the exponential phase, possibly caused by shear damages imposed on the producing cells at the high stirring in the 50 L culture. The scale-up process could be successfully achieved if a scale-up criterion of constant oxygen mass transfer coefficient (k_La) is applied to the 50 L pilot-scale bioreactor system.

• Korean Chemical Engineering Research
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• v.52 no.4
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• pp.467-474
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• 2014

Preliminary design in chemical process furnishes economic feasibility through calculation of both mass balance and energy balance and makes it possible to produce a desired product under the given conditions. Through this design stage, the process possesses unchangeable characteristics, since the materials, reactions, unit configuration, and operating conditions were determined. Unique characteristics could be very economic, but it also implies various potential risk factors as well. Therefore, it becomes extremely important to design process considering both economics and safety by integrating process simulation and quantitative risk analysis during preliminary design stage. The target of this study is LNG liquefaction process. By the simulation using Aspen HYSYS and quantitative risk analysis, the design variables of the process were determined in the way to minimize the inherent explosion risks and operating cost. Instead of the optimization tool of Aspen HYSYS, the optimization was performed by using stochastic optimization algorithm (Covariance Matrix Adaptation-Evolution Strategy, CMA-ES) which was implemented through automation between Aspen HYSYS and Matlab. The research obtained that the important variable to enhance inherent safety was the operation pressure of mixed refrigerant. The inherent risk was able to be reduced about 4~18% by increasing the operating cost about 0.5~10%. As the operating cost increases, the absolute value of risk was decreased as expected, but cost-effectiveness of risk reduction had decreased. Integration of process simulation and quantitative risk analysis made it possible to design inherently safe process, and it is expected to be useful in designing the less risky process since risk factors in the process can be numerically monitored during preliminary process design stage.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.9
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• pp.961-976
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• 2006

Designing water-reuse network which can reduce the fresh water within the process and increase the water-use efficiency by scientific and systematic analysis is recently interested in the industries. Water systems often allow efficient water uses via water reuse and recirculation in the paper, petrochemical, and steel industries which necessitate a lot of freshwater within the process. Defining network layout connecting water-using process is frequently accomplished by using water pinch technology which optimizes freshwater entering the process and also reduces the wastewater. In this review, recent researches and case studies of water pinch technology which can find the bottleneck of the water stream at the water reuse designing stage are introduced. Necessity of water pinch technology is illustrated by examples of real industries. Recent studies on simultaneous energy and water minimization and water-reuse network among industries in eco-industrial park(EIP) are also introduced.

• Tunnel and Underground Space
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• v.15 no.4 s.57
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• pp.275-287
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• 2005

At the planning and design stages of a development of underground space or tunneling project, the information regarding ground conditions is very important to enhance economical efficiency and overall safety In general, the information can be expressed using RMR or Q-system and with the geophysical exploration image. RMR or Q-system can provide direct information of rock mass in a local scale for the design scheme. Oppositely, the image of geophysical exploration can provide an exthaustive but indirect information. These two types of the information have inherent uncertainties from various sources and are given in different scales and with their own physical meanings. Recently, RMR has been estimated in unsampled areas based on given data using geostatistical methods like Kriging and conditional simulation. In this study, simulated annealing(SA) is applied to overcome the shortcomings of Kriging methods or conditional simulations just using a primary variable. Using this technique, RMR and the image of geophysical exploration can be integrated to construct the spatial distribution of RM and to evaluate its uncertainty. The SA method was applied to solve an optimization problem with constraints. We have suggested the practical procedure of the SA technique for the uncertainty evaluation of RMR and also demonstrated this technique through an application, where it was used to identify the spatial distribution of RMR and quantify the uncertainty. For a geotechnical application, the objective functions of SA are defined using statistical models of RMR and the correlations between RMR and the reference image. The applicability and validity of this application are examined and then the result of uncertainty evaluation can be used to optimize the tunnel layout.

• Microbiology and Biotechnology Letters
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• v.41 no.2
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• pp.145-152
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• 2013

The culture conditions to maximize the production of endoglucanase (EC 3.2.1.4) from the brown rot fungus Fomitopsis pinicola MKACC 54347 mycelia were investigated. Among the tested media for endoglucanase production, Mandel's mineral salts medium (MSM; 1% cellulose, 0.1% peptone, 0.14% $(NH_4)_2SO_4$, 0.03% urea, 0.2% $KH_2PO_4$, 0.03% $MgSO_4{\cdot}7H_2O$, 0.03% $CaCl_2$, and 0.1% trace metal solution (19.8 mM $FeSO_4$, 13.0 mM $MnSO_4$, 12.2 mM $ZnSO_4$, and 15.4 mM $CoCl_2$)) produced the highest activity of the enzyme. To optimize the medium composition for enzyme activity, the effects of various carbon, nitrogen, phosphorus, and inorganic sources were investigated in MSM. Maximal enzyme production was accomplished using a medium containing 2% carboxymethyl cellulose (CMC), 2% yeast extract, 0.2% $KH_2PO_4$, 0.03% $MnSO_4$, and 0.3% trace metal solution. Different physiological conditions, like incubation period and temperature, were also examined to assess their influence on enzyme production. Enzyme production from F. pinicola reached its highest level after cultivation for 8 days at $25^{\circ}C$. Nondenaturing polyacrylamide gel electrophoresis (PAGE), followed by the endoglucanase activity staining using CMC as the substrate, was performed to identify the endoglucanase under the culture conditions studied. Zymogram analysis of the culture supernatant revealed an endoglucanase band with a molecular mass of 52 kDa. The optimum pH and temperature for enzyme activity were $55^{\circ}C$ and pH 5.0, respectively.

• Journal of Digital Contents Society
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• v.9 no.4
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• pp.661-667
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• 2008

The dynamics of business cycles has been changed by the macroscopic economic forces because of the introduction of new technical know-how each year. These the dynamics of business has a significant influence on the investment of enterprise in the information communication field. Today, the most important goal of the IT investment is simply not to lower the production cost any more, but to improve the usefulness for the customers and partners in order to obtain the optimized mass products. Therefore, the enterprises have been concentrating their all abilities on the automation, integration, and optimization of business process using BPM. In addition, they are concentrating their efforts on the business expansion by approaching the technical aspect using RFID application system. However, in order to accomplish a successful enterprise ability, the technical view, business process view, and organization view must be considered together. We suggested the method considering organization view, via the technical element, i.e., RFID system for approaching the business process. Furthermore, we tried the optimization of assignment using Context Analysis methodology and proposed the method to reduce the element with respect to the time, human, and expense by applying the Case Study method that minimizes the iteration times through the transmitted processing procedure and type. The proposed method gave us the expectation that it will bring out the innovative improvement with respect to the time, expense, quality, and customer's satisfaction in the process from the analysis of business process to the analysis and design of system.

• Journal of Power Electronics
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• v.11 no.4
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• pp.408-417
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• 2011

The plug-in hybrid electric vehicles (PHEVs) are specialized hybrid electric vehicles that have the potential to obtain enough energy for average daily commuting from batteries. The PHEV battery would be recharged from the power grid at home or at work and would thus allow for a reduction in the overall fuel consumption. This paper proposes an integrated power electronics interface for PHEVs, which consists of a novel Eight-Switch Inverter (ESI) and an interleaved DC/DC converter, in order to reduce the cost, the mass and the size of the power electronics unit (PEU) with high performance at any operating mode. In the proposed configuration, a novel Eight-Switch Inverter (ESI) is able to function as a bidirectional single-phase AC/DC battery charger/ vehicle to grid (V2G) and to transfer electrical energy between the DC-link (connected to the battery) and the electric traction system as DC/AC inverter. In addition, a bidirectional-interleaved DC/DC converter with dual-loop controller is proposed for interfacing the ESI to a low-voltage battery pack in order to minimize the ripple of the battery current and to improve the efficiency of the DC system with lower inductor size. To validate the performance of the proposed configuration, the indirect field-oriented control (IFOC) based on particle swarm optimization (PSO) is proposed to optimize the efficiency of the AC drive system in PHEVs. The maximum efficiency of the motor is obtained by the evaluation of optimal rotor flux at any operating point, where the PSO is applied to evaluate the optimal flux. Moreover, an improved AC/DC controller based Proportional-Resonant Control (PRC) is proposed in order to reduce the THD of the input current in charger/V2G modes. The proposed configuration is analyzed and its performance is validated using simulated results obtained in MATLAB/ SIMULINK. Furthermore, it is experimentally validated with results obtained from the prototypes that have been developed and built in the laboratory based on TMS320F2808 DSP.