• 한국균학회소식:학술대회논문집
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• 한국균학회 2009년도 추계학술대회 및 정기총회
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• pp.76-95
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• 2009

In the fungal genus Cordyceps, the type species Cordyceps militaris produces bioactive ingredients and exhibits medicinal effects as a Traditional Chinese Medicine (TCM), The fruiting bodies of C.militaris have now been mass-produced artificially and used as functional food and medicine in China. The unstable variation in forming fruiting body is however a key restrictive factor in industrial production. The genetic study on in vitro stromata formation of C. militaris has rarely been carried out. Here, we report the effects of genetic variation including the mating system on perithecial stromata formation of C. militaris. Monoconidial isolates which have both MAT1-1-1 and MAT1-2-1(genotype MAT1-1/2) could produce stromata. While the isolates only have either MAT1-1-1 or MAT1-2-1 (genotype MAT1-1 or MAT1-2) failed to produce stromata. Despite obvious heterothallism, homothallism was occasionally observed in a few isolates of C. militaris. High genetic variation was observed amongst the different monoconidial isolates of C. militaris. The unstable variation or lose of fruiting body formation was found to be caused by the inner-species high genetic variation of C. militaris. These results also indicated that C. militaris sexually behaved as both heterothallic and homothallic and required two mating type compatible in the same culture in order to produce regular clubshaped perithecial stromata.

• 대한가정학회지
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• 제33권4호
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• pp.65-87
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• 1995

The purpose of this study was to investigate the consciousness, knowledge and food preferences for the Korean traditional foods, using a questionnaire survey for 560 high school students in seoul. The data were analyzed with simple frequency, percentage, t-test, chi-square test, dispersion analysis, Pearson's correlation and hierarchical multiple-regression analysis using the SPSS/PC+ Package system. The results of the survey are summarized as follows ; Girls showed a more interest in Korean traditional foods than boys. The motive of having interest in korean traditional foods were through mass communication or home education. Most of students were preffered korean traditional foods and were proud of native foods. The most preffered foods were Kim-chi and beverage. and they thought that color, appearance, foodstuff combination of Korean traditional foods were better than those of foreign foods. they showed a tendency to decrease the use Korean traditional foods because it is difficult to cook and they have little time to cook. They have taken more Korean traditional foods at home. Most students were responded that it is necessary to develope Korean traditional foods at home for the succeeding of korean dietary life culture. For the idea of improving Korean traditional foods, it is thought that simplified cooking method, new cooking method and fastfood is developed.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.220-223
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• 2002

본 연구에서는 자체 제작한 2L 규모의 bubble-column photobioreactor에서 H. pluvialis의 배양을 시도하였고 astaxanthin의 축적량 증가를 유도하기 위하여 배양시작 20일 후에 light stress를 주었다. 이 방법을 통하여 대조구에 비하여 68%의 건조균체중량 증가와 215%의 astaxanthin 축적량 증가를 유도할 수가 있었고, bubble-column photobioreactor를 이용한 유도배양이 H. pluvialis의 배양에 적합함을 알 수가 있었다.

• Journal of Ginseng Research
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• 제23권3호
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• pp.148-163
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• 1999

The efficacy of three auxins, viz. 2,4-0, NAA and dicamba, were compared for the induction of somatic embryogenesis in American ginseng (Panax quinquefolium L.). Somatic embryos (SEs) formed on ginseng cotyledonary, zygotic embryo and shoot explants after 8 weeks of induction by the auxin stimuli. Significantly more somatic embryos were induced by culture of any of the ginseng explants on media supplemented with $5{\mu}M$ 2,4-0 than any other auxin treatment. Shoots derived from somatic embryos had the greatest regenerative potential and zygotic embryos the least. Explants generated from green (unstratified) seeds gave similar or higher frequency of embryogenesis as the explants derived from stratified seeds. Histological and SEM studies confirmed that the regenerimts were somatic embryos. Somatic embryos germinated and developed into normal plants in $3\~6$ months. About $10\%$ of plantlets from second generation SEs formed flowers within 10 weeks, particularly on media supplemented with $GA_3$ The development of a regeneration system for ginseng through somatic embryogenesis is a necessary first step for mass propagation and genetic improvement of American ginseng.

• Journal of Dairy Science and Biotechnology
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• 제41권1호
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• pp.34-43
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• 2023

Hydrolysis of whey-derived proteins using lactic acid bacteria (LAB) utilizes the mass culture method and fermentation of LAB to produce effective bioactive peptides. Whey protein has the biological potential of its precursors, but the active fragments may not be released depending on the hydrolysis method. As an alternative to these problems, the nutritional and bioactive functionality of the hydrolysis method have been reported to be improved using LAB for whey protein. Peptide fractions were obtained using a sample fast protein liquid chromatography device. Antioxidant activity was verified for each of the five fractions obtained. In vitro cell experiments showed no cytotoxicity and inhibited nitric oxide production. Cytokine (IL [interleukin]-1α, IL-6, tumor necrosis factor-α) production was significantly lower than that of lipopolysaccharides (+). As a result of checking the amino acid content ratio of the fractions selected through the AccQ-Tag system, 17 types of amino acids were identified, and the content of isoleucine, an essential amino acid, was the highest. These properties show their applicability for the production of functional products utilizing dietary supplements and milk. It can be presented as an efficient method in terms of product functionality in the production of uniform-quality whey-derived peptides.

• Journal of Microbiology and Biotechnology
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• 제16권11호
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• pp.1706-1712
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• 2006

Because of the need for new antimicrobial substances with novel mechanisms of action, we report here the use of an Acinetobacter reporter system for high-throughput screening of active antimicrobial agents. The bioreporter Acinetobacter strain DF4/PUTK2 carrying luciferase genes luxCDABE was chosen because of its ecological importance and it is widespread in nature. This bioreporter is genetically engineered to emit light constitutively that can be measured in real time by luminometry. Hence, this reporter system was employed to determine the bacteriostatic actions of spent-culture supernatants derived from twelve bacterial isolates. Out of the results, the strongest bioluminescence inhibitory effect of the supernatants was recorded with Bacillus cereus strain BAC (S5). Subsequently, ethyl acetate extracts of extracellular products of strain BAC (S5) were separated by a thin-layer chromatography (TLC). Based on the bioluminescence inhibitory assay, three fractions were found to have antimicrobial activity. One fraction (C) having the strongest antimicrobial activity was further purified using TLC and characterized by IR, $^1H$ NMR, mass spectrometry, SDS-PAGE, and amino acid composition analysis. The results predicted the presence of 2-pyrrolidone-S-carboxylic acid (PCA) and the octadeconic-acid-like fatty acid. Fraction C also demonstrated a broad inhibitory activity on several Gram-negative and Gram-positive bacteria. In conclusion, the Acinetobacter reporter system shows great potential to be a reliable, sensitive, and real-time indicator of the bacteriostatic actions of the antimicrobial agents.

• 한국의상디자인학회지
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• 제20권3호
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• pp.27-32
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• 2018

Hanbok is a our own history and tradition and is an icon of Korean history and culture. Today, the costumes are moving around the world fashion trend due to the development of mass media and internet at the same time. This is an important clue to marketing activities and can be used for predictive analysis. Although Hanbok is changing little by little every year, research on the trend of Hanbok is rare. This study analyzed the results of searching for 'Hanbok' as a keyword in Portal Site Naver and posting a customized purchase of Hanbok for marriage between 2016 and 2017. The analysis was based on analyzing the photos uploaded, and analyzing purchase reason in the On-line review. Most buyer of Hanbok purchased for prepare marriage. The choice of a customized hanbok is mostly to search online or to use the fair. The most important factor in choosing a custom Hanbok that appeared in online reviews is color and then price. The color of the jacket is mostly light color and the off-white color is the most used and the long skirt such as the pink system, the chorale system and the red system, and it can be seen that the pink skirt is overwhelmingly large. In the design of Hanbok, The sleeves were straight and narrow, and the length was the chest line. The collar were enlarged and widened. 고름 used the sole color instead of the jacket and skirt color, and it was narrow not long. skirt's pleats was wide, and designed to overlap with double color of the fabric.

• 한국의상디자인학회지
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• 제4권2호
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• pp.181-196
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• 2002

The functions of the clothing have been changed rapidly in modern society. That means people want to show oneself strongly by the clothing. In other words, the social aspect of the clothing's function which is the expression of own's personality and satisfaction one's desire is emphasized. This study is aimed at taking a look at the functions of modern clothing. The modern clothing has the various functions which are related to the changing of the social system. The result of the study are as follows: With the differentiation of society and institutionalization, the clothing is recognized as the medium which shows one's status and is restricted by people. In modern society as mass society, the system of the social position collapsed. People has the inclination to express own's consciousness, personality and existence intensely. Also, the functions of clothing became diverse. That means the clothing is influenced by the human's consciousness, a sense of values and became the medium which reflects the people's life. This change of the costume means the clothing focus on the functional factor of the free-style clothing getting out of rational value. Therefore, the change of clothing is not the change of the design but the change of value of the clothing, one's way of thinking about the clothing, the change of human life in whole society and the culture. With the changing way of life, the change of the clothing has the system in which the design changes. Therefore, I found that the we have to take a look not the change of the factors which influence the fashion but the functions of the clothing itself.

• 한국콘텐츠학회논문지
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• 제10권12호
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• pp.254-266
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• 2010

본 연구는 천안시를 대상으로 도시교통문제를 다루었다. 본 연구는 문헌조사와 실증조사를 병행하였다. 회수된 설문지 394부를 SPSS 15.0버전으로 빈도분석을 실시하였다. 천안시의 도시교통문제는 출 퇴근시의 교통체증 현상, 대중교통의 불편, 주차문제, 자동차로 인한 대기오염, 교통사고로 인한 불안 등을 들 수 있다. 교통문제의 개선방안으로는, 첫째, 합리적 교통수요의 관리에 따른 교통체계의 개선방안이 마련되어야 한다. 둘째, 천안시는 대중교통의 이용대상인 대학생이 많으므로 대학생을 대상으로 할인을 적용 대중교통 이용률을 높여야 한다. 셋째, 대중교통중심체제의 강화 및 개선을 통한 교통체증의 해결이 필요하다. 넷째, 도로 등 교통시설의 공급확대, 가로망체계 정비 및 신호운영체계의 개선을 적극적으로 추진해 나가야 한다. 특히 천안에는 오래된 도로의 정비가 필요하고 도로망이 복잡하게 얽혀있는데 이를 간단하게 개선함으로써 보다 접근성이 좋아질 것이다. 다섯째, 주차문제의 해결과 이용자들의 교통질서 의식함양 등 선진 교통문화의 정착이 필요하다.

• Journal of Plant Biotechnology
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• 제49권4호
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• pp.325-330
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• 2022

본 연구는 국내에서 소비되는 약용작물 중 경제적 가치는 높으나 국내 생산량이 적고 수입 의존도가 높은 Atractylodes의 식물 조직 배양 시스템을 구축하기 위해 수행되었다. 삽주는 A. ovata를 사용하였고, 4가지 cytokinins류, 6-benzylaminopurine (BA), zeatin, kinetin, thidiazuron (TDZ)을 2가지 농도(0.5, 1.0 mg/L)로 처리하였다. 4가지 유형의 cytokinin 중 BA처리는 A. ovata의 신초유도와 뿌리 생육에 효과적이었다. 0.5 mg/L 및 1.0 mg/L BA 모두 BA 처리에서 유사한 결과를 나타내었지만 1.0 mg/L BA가 신초와 뿌리 생육을 촉진하는데 더 효과적이었다. 처리 중 신초의 개수와 뿌리의 생중량(FW)을 제외하고는 TDZ 처리가 신초와 뿌리생육에 효과적이지 않아 본 수종에서는 적합하지 않았다. 본 연구에서는 A. ovata의 아배양을 이용한 기내증식 시스템을 구축하였다. 위 결과는 기내 재분화를 이용한 산림약용자원 A. ovata의 안정적인 생산 및 증식을 위한 기반 기술로 사용될 것으로 사료된다.