• Microbiology and Biotechnology Letters
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• v.27 no.4
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• pp.327-332
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• 1999

A highly productive fed-batch fermentation process was developed for the production of L-ornithine by using a new stabilized strain, Breviabcterium ketoglutamicum BK52. Fed-batch cultures with a continuous feeding of the complex medium were conducted on various operating conditions. The optimal concentration of phosphate in the complex medium was 2.1g/L. The optimal feeding rate of L-arginine was 0.028g/L/hr. The optimal feeding point of the complex medium was determined to be at 40 OD of the cell mass. The final L-ornithine concentrations within 64hrs of cultivation in 5 and 50 liter fermenters were 73g/L and 71g/L, respectively. The maximum overall L-ornithine productivity was 1.14g/L/hr which was about 2 times higher than that of the conventional fed-batch culture with intermittent feeding. The overall productivity of the fermentation system is remarkably improved by employing the optimized conditions, and it offers a significant potential for industrial application.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.22 no.6
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• pp.337-344
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• 2010

Good plant-growth conditions can be achieved by means of using greenhouses. One of the main issues in greenhouse cultivation is energy savings through the development of high efficient heating and cooling system. GSHPs are one of the recommended systems to cope with this pending need. The aim of this study is to investigate the heating performance of ground source multi-heat pump system installed in a greenhouse under part load conditions. Daily average heating COP of the heat pump unit was very high by at least 7.4, because of relatively large condenser, evaporator, and mass flow rate through ground loop heat exchanger. However, the system COP, overall heating coefficient of the performance of the system with heat pump unit and GLHX, decreased drastically due to relatively large power consumption of circulating pump under part load condition. It is suggested that the technology to enhance the performance of the ground source multi-heat pump system for a greenhouse under part load conditions should be developed.

• Journal of Wetlands Research
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• v.10 no.3
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• pp.1-7
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• 2008

In this study, the nutrient removal capacity of paddy field was investigated. Paddy field was considered as a constructed wetland. The present study was conducted during a cropping period in a rice cultivation area located in Hampyung-gun, Jeollanamdo, Korea. Water balance and mass balance of nutrients were estimated after intensive field monitoring. The surface runoff losses of T-N and T-P were 20.82 kg/ha and 1.55 kg/ha, respectively. The losses were 13.2% and 6.4% of T-N and T-P input into paddy fields, respectively. The results showed that the paddy fields have nutrient removal function.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.6
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• pp.357-361
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• 2002

Continuous production of lactic acid from glucose by Lactobacillus rhamnosus with cell recycling using an acoustic cell settler was carried out. The performance of the system, such as the concentration of cell and product were compared with the control experiment without recycling. The acoustic settler showed cell separation efficiency of 67% during the continuous operation and the cell concentration in the fermentor with recycle exceeded that of the control by 29%. Com-pared with the control, tactic acid production was increased by 40%, while glucose consumption was only increased by 8%. The higher value of lactic acid production to substrate consumption (Yp/s, product yield coefficient) achieved by cell recycling is interpreted to indicate that the recycled cell mass consumes less substrate to produce the same amount of product than the control Within system environmental changes due to the longer mean cell residence time induced the cells maintaining the metabolic pathways to produce Less by-Product but more product, lactic acid.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.233-236
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• 2000

Bacillus thuringiensis (Bt) is the most widely used microbial insecticide in the biological control market. Cultivation of the microorganism to high cell densities offers potential for enhancing the rate of formation as well as the concentration of the desired products In the fermentation broths in bioreactor. With this objective, we developed the new bioreactor incorporating ceramic membrane module for the retention of cell mass. Cell yield and spore formation of Bacillus thuringiensis was improved markedly by adopting this new bioreactor based on glucose -limited feeding operation. It was possible to grow the cell and the heat-resistant spore to above $1.2\;{\times}\;10^{10}\;CFU/ml$ density. With glucose-limited operation, we studied the growth behavior of Bacillus thuringiensis during the cell retention culture. Linear growth of Bacillus thuringiensis was observed under glucose-limited culture, which matched well with simple mathematical model of cell retention culture.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.220-223
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• 2002

This study investigated a lab-scale inducing method for efficient astaxanthin accumulation. As a model system. Haematococcus pluvialis was cultivated in 2 liter bubble-column photobioreactors. The astaxanthin - inducing results using high light irradiation were compared with that of the control experiment under standard irradiation (40 ${\mu}E/m^2/s$). After the late linear growth phase (> 20 days). high light energy (230 ${\mu}E/m^2/s$) was supplied to the culture broth for astaxanthin induction. As a result. the dr γ cell weight and the astaxanthin productivity were increased up to 68% and 215%, respectively. higher than those of the control experiment. This result indicates that bubble-column type photobioreactor is a good candidate for mass cultivation of H. pluvialis and high light irradiation is an efficient induction method for astaxanthin accumulation in lab-scale bubble-column photobioreactors.

• Journal of The Korean Society of Agricultural Engineers
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• v.51 no.1
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• pp.33-39
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• 2009

A number of fallow paddies field continues to increase because of adverse agricultural circumstance such as upsurge income of foreign products, increase of labor costs in local community, imbalance between productivity and profitability etc. Such fallow paddies are necessary to be protected against weeds and trees. If fallow paddies are not managed, it will be devastated and needed cost for re-cultivation. Therefore, management of gradually increased fallow paddy fields should be conducted. In this study, the effect of plants growth inhibition and water quality purification through the control of flood in fallow paddy were examined in an experimental field. The managed and unmanaged fallow paddies through the control of water depth were constructed in the experimental field. The monitoring was conducted from 2005 to 2007. As the result, the managed fallow paddies were more effective than the unmanaged ones on the growth inhibition and water purification. In addition, when the fallow paddy is managed with regular water depth, it was the most effective in the plants growth inhibition.

• Journal of the Korean Society of Tobacco Science
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• v.29 no.2
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• pp.118-124
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• 2007

GRLs(Guidance Residue Levels) of agricultural chemicals for tobacco are recommended by the CORESTA Agro-Chemical Advisory Committee guide. In the GRLs list, organochlorine group is one of pesticides commonly used on tobacco cultivation. In this model study, the quantitative correlation in the transfer rate of pesticide residue into tobacco smoke by spiking of organochlorine pesticides to cigarette and pyrolysates were investigated. The spiking concentration referred to the range of GRLs list and the organochlorine pesticides in mainstream smoke were analyzed by GC-MS. For the understanding of the composition variation versus temperature, the behavior of pesticides was investigated by pyrolysis-gas chromatography-mass spectrometry(Py-GC-MS). In this study, the transfer rate of pesticide residue into tobacco smoke at four different spiking concentration and the composition of pyrolysates were analyzed differently. At $10\;{\mu}g/cig$ spiking concentrations, the organochlorine pesticides were transferred into tobacco smoke in $0.02\;{\sim}\;10.19\;%$ each of component and the most of pesticides were pyrolyzed during smoking. It was found that the decomposition compounds from organochlorine pesticides were mainly composed of oxygenated and nitrogenous compounds. This study could estimate that the transfer rate of pesticides into tobacco smoke is very small amount.

• Journal of Aquaculture
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• v.10 no.2
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• pp.199-211
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• 1997

Effects of light and temperature on the early growth of Hizikia fusiformis were investigated for establishment of artificial seed production. Young germlings were cultured for 70 days under different culutre conditions : light intensities (500, 1, 000, 2, 000, 4, 000, 8, 000 lux), photoperiods (8 : 16, 12 : 12, 16 : 8, (L : D)) and temperatue (10, 15, 20, 25, $30^{\circ}C$). Growth of the germlings was facilitated in 2, 000~4, 000 lux of light intensities, 16 : 8 (L:D) of photoperiods and 15~$20^{\circ}C$ of temperature. Culture conditions were revealed for the mass culture of artificial seeds of effective early growth in Hizikia cultivation.

• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.489-498
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• 2013

A new Bacillus strain degrading starch, named Bacillus sp. UEB-S, was isolated from a southern Tunisian area. Amylase production using solid-state fermentation on millet, an inexpensive and available agro-resource, was investigated. Response surface methodology was applied to establish the relationship between enzyme production and four variables: inoculum size, moisture-to-millet ratio, temperature, and fermentation duration. The maximum enzyme activity recovered was 680 U/g of dry substrate when using $1.38{\times}10^9$ CFU/g as inoculation level, 5.6:1 (ml/g) as moisture ratio (86%), for 4 days of cultivation at $37^{\circ}C$, which was in perfect agreement with the predicted model value. Amylase was purified by Q-Sepharose anion-exchange and Sephacryl S-200 gel filtration chromatography with a 14-fold increase in specific activity. Its molecular mass was estimated at 130 kDa. The enzyme showed maximal activity at pH 5 and $70^{\circ}C$, and efficiently hydrolyzed starch to yield glucose and maltose as end products. The enzyme proved its efficiency for digesting raw cereal below gelatinization temperature and, hence, its potentiality to be used in industrial processes.