Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Continuous Cultivation of Lactobacillus rhamnosus with Cell Recy-cling Using an Acoustic Cell Settler

  • Yang, Yun-Jeong (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University) ;
  • Hwang, Sung-Ho (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University) ;
  • Lee, Sang-Mok (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University) ;
  • Kim, Young-Jun (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University) ;
  • Koo, Yoon-Mo (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Continuous production of lactic acid from glucose by Lactobacillus rhamnosus with cell recycling using an acoustic cell settler was carried out. The performance of the system, such as the concentration of cell and product were compared with the control experiment without recycling. The acoustic settler showed cell separation efficiency of 67% during the continuous operation and the cell concentration in the fermentor with recycle exceeded that of the control by 29%. Com-pared with the control, tactic acid production was increased by 40%, while glucose consumption was only increased by 8%. The higher value of lactic acid production to substrate consumption (Yp/s, product yield coefficient) achieved by cell recycling is interpreted to indicate that the recycled cell mass consumes less substrate to produce the same amount of product than the control Within system environmental changes due to the longer mean cell residence time induced the cells maintaining the metabolic pathways to produce Less by-Product but more product, lactic acid.

Keywords

References

  1. J. Membrane Sci. v.114 Membrane bioreactor for lactic acid production. Moueddeb H.;J. Sanchez;C. Bardot;M. Fick https://doi.org/10.1016/0376-7388(95)00307-X
  2. J. Biotechnol. v.41 Kinetic and stability of a fibrous-bed bioreactor for continuous production of lactic acid from unsupplemented acid whey. Silva, E. M.;S.-T. Yang https://doi.org/10.1016/0168-1656(95)00059-Y
  3. Ultrasonics Sonochem. v.7 A comparative study between classical stirred and ultrasonically-assisted dead .end ultrafiltration. Simon, A.;L. Penpenic;N. Gondrexon;S. Taha;G. Dorange https://doi.org/10.1016/S1350-4177(00)00039-0
  4. J. Microbiol. Met. v.27 Ultrasonic standing wave removal of microorganisms from suspension in small batch system. Hawkes, J. J.;M. S. Limaye;W. T. Coakley https://doi.org/10.1016/S0167-7012(96)00951-7
  5. Enzyme Microb. Technol. v.19 A Continuous flow ultrasonic cell .filtering method. Hawkes, J. J.;W. T. Coakley https://doi.org/10.1016/0141-0229(95)00172-7
  6. Acoustica v.82 Ultrasonic separation of suspended particles: Part III. Application in Biotechnology. Groschl, M.;W. Burger;B. Handle
  7. J. Appl. Microbiol. v.82 Filtration of bacteria and yeast by ultrasound-enhanced sedimentation. Hawkes, J. J.;M. S. Limaye;W. T. Coakley https://doi.org/10.1111/j.1365-2672.1997.tb03295.x
  8. The 219th ACS National Meeting. Ultrasonic cell separation . Production of monoclonal antibodies in continuous perfusion cultures. Heine, H.;C. Y. Arod;A. R. Bernard;H. D. Blasey
  9. Biotechnol. Bioprocess Eng. v.4 Ultrasonic cell separator as a cell retaining device for high density cultures of plant cell. Shin, M. K., K. M. Park, and G. H. Cho https://doi.org/10.1007/BF02933750
  10. Biotechnol. Bioeng. v.59 High-density perfusion culture of insect cell with a biosep ultrasonic filter. Zhang, J.;A. Collins;M. Chen;I. Knyazev;R. Gentz https://doi.org/10.1002/(SICI)1097-0290(19980805)59:3<351::AID-BIT11>3.0.CO;2-H
  11. Biotechnol. Prog. v.10 A novel ultrasonic resonance field device for the retention of animal cells. Doblhoff-Dier, O.;T. Gaida;H. Katinger;M. GrOschl;W. Burger;B. Handl https://doi.org/10.1021/bp00028a600
  12. Bio/Technology v.12 Acoustic cell filter for high density perfusion culture of hybridoma cells. Trampler, F.;S. A. Sonderhoff;P. W. S. Pui;D. G. Kilburn;J. M. Piret https://doi.org/10.1038/nbt0394-281
  13. Appl. Microbiol. Biotechnol. v.57 Continuous lactic acid fermentation using a plastic composite support biofilm reactor. Cotton J. C.;A. L. Pometto;J. Gvozdenovic-Jeremic
  14. J. Ferment. Bioeng v.84 Effect of B vitamin supplementation on lactic acid production by Lactobacillus casei. Yoo, I. K.;H. N. Chung;E. G. Lee;Y. K. Chang;S. H. Moon https://doi.org/10.1016/S0922-338X(97)82551-2
  15. Trends Biotechnol. v.15 Ultrasonic separation in analytical biotechnology. Coakley, W. T. https://doi.org/10.1016/S0167-7799(97)01122-0
  16. Ultrasonics v.38 Ultrasound conditioning of suspensions- studies of steaming influence on particle aggregation on a lab- and pilot-scale. Spengler, J;M. Jekel https://doi.org/10.1016/S0041-624X(99)00145-6
  17. Bioseparation v.4 Ultrasound enhanced phase partition of microorganism. Allman, R.;W. T. Coakley
  18. HWAHAK KONGHAK v.39 Effects of operation and design parameters on the recovery of microorganism and particles in ultrasonic sedimentation. Hwang, S.-H.;Y.-M. Koo
  19. Appl. Microbiol. Biotechnol. v.51 Continuous production of L(+)-lactic acid by Lactobacillus casei in two-stage system. Bruno-Barcena J. M.;A. L. Ragout;P. R. Cordoba;F. Sineriz https://doi.org/10.1007/s002530051397
  20. Biotechnol. Bioeng. v.73 High-rate continuous production of lactic acid by Lactobacillus rhamnosus in a two-stage membrane cell-recycle bioreactor. Kwon, S. H.;I.-K. Yoo;W. G. Lee;H. N. Chang;Y. K. Chang https://doi.org/10.1002/1097-0290(20010405)73:1<25::AID-BIT1033>3.0.CO;2-N
  21. Biotechnol. Bioeng. v.67 Enhanced production of L-(+)-lactic acid in chemostat by Lactobacillus casei DSM20011 using ion-exchange resins and cross-flow filtration in a fully automated pilot controlled via NIR. A. Gonzalez-Varay R.;G. Vaccari;E. Dosi;A. Trilli;M. Rossi;D. Matteuzzi