• Title/Summary/Keyword: mass breeding

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Development of SNP Markers for Domestic Pork Traceability (국내산 돼지고기의 원산지 검증을 위한 SNP Marker Set 개발)

  • Kim, Sang-Wook;Li, Xiaoping;Lee, Yun-Mi;Kim, Jong-Joo;Kim, Tae-Hun;Choi, Bong-Hwan;Kim, Kwan-Suk
    • Journal of Animal Science and Technology
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    • v.52 no.2
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    • pp.91-96
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    • 2010
  • The purpose of the study was to develop an optimum SNP marker set to be utilized for domestic pork traceability. The study tested 51 SNP markers analyzed for origin of farm to be determined from genotypes of offspring and parents in pigs. With the simulation data through random mating population (PI), half sib mating population ($PI_{half-sib}$) and full sib mating population ($PI_{sibs}$), probability of identical genotypes were analyzed as $5.63{\times}10^{-33}$, $4.35{\times}10^{-15}$ and $1.32{\times}10^{-15}$, respectively. The 51 SNP markers also had 100% accuracy for parental determination. These results suggest that if the pig breeding stock is genotyped with the 51 SNP markers, the genotype information of individual offspring can be checked for farm origins by tracing parental sow and sire. Therefore, these SNP markers will be useful to trace the pork from production to consumption in pigs.

State of Optimal Rearing Technique on the Abalone (Haliotis discus hannai) Juvenile (참전복 치패의 최적사육 기술현황)

  • Son, Maeng-Hyun;Lee, Jung-Uie;Park, Min-Woo;Lim, Han-Kyu;Kim, Dae-Jung;Hwang, Hyung-Gue
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.6
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    • pp.621-627
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    • 2009
  • In order to standardize the juvenile abalone rearing technique, we selected sample farms by region in East, West and South coasts of Korea and Jeju island. We also have reviewed previous literature and visited farms to survey on the management of abalone juvenile production, spawning, hatching and so forth. Results of investigation are as follows: The light colors of tanks for larvae breeding are good for a frequent examination of larvae behaviour changes during the breeding period. The tank for the abalone juvenile production is a rectangular form in general and its size should amount to 3.5 m in length and 1.2 m width. It also should be built with proper drainage. The best age and size of adult for juvenile production are 3-6 years old individuals, with 9-12 cm separate burial and 125-150 g average weight. To induce spawning, the use of the exposure on air and ultraviolet ray together was the most effective. The density of larvae by plate should be 150-300 individuals and the proper stocking density was est imated and amount to 10-30 individuals. It has been shown that a correlation between water surface size($X_1$) and number of plates ($Y_1$), when producing abalone juveniles, is quite high and it is described by equation $Y_1=138.88X_1-5,736.8\;(R^2=0.9028)$. In addition, it has also been shown that a correlation between production of abalone juveniles ($Y_2$) and number of plates ($X_2$) is high and it is described by equation $Y_2=4.554X_2+12,493\;(R^2=0.8818)$. In Jindo region where a mass production of juveniles abalone has been done, it was shown, that a correlation between rearing water surface size ($X_3$) and production of juveniles abalone ($Y_3$) is very high and this relationship was described by the equation $Y_3=747.03X_2+94,359(R^2=0.9809)$. It has also been shown that a correlation between water surface size ($X_4$) and production of abalone juveniles ($Y_4$) in nationwide is high and the relationship between this variables was described by equation $Y_4=635.85X_4+99,923\;(R^2=0.9020)$.

In vitro Culture of Adventitious Roots from Dioscorea nipponica Makino for the Production of Steroidal Saponins (부채마 (Dioscorea nipponica Makino)의 부정근 배양조건과 Steroidal Saponin의 기내생산)

  • An Jung-Hee;Son Kun-Ho;Sohn Ho-Yong;Kwon Soon-Tae
    • Journal of Plant Biotechnology
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    • v.32 no.3
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    • pp.217-223
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    • 2005
  • Effects of growth regulators on growth of adventitious roots and accumulation of steroidal saponins, such as dioscin, prosapogenin A and prosapogenin C, in cultures of Dioscorea nipponica were determined. The maximum growth of adventitious roots was observed in MS medium supplemented with 30 g/L sucrose and 1.0 mg/L NAA. Addition of BA in combination with NAA appeared to be no effective in the growth of adventitious roots. Among the twenty different adventitious roots formed from different seeds, strain No. 10 was selected based on production ability of dioscin, and its stability through the successive liquid culture. During the first 4 weeks of incubation, contents of steroidal saponins in adventitious roots were negligible but the contents were markedly increased at 5 weeks of incubation. Dioscin and prosapogenin C content in IBA-treated adventitious roots were significantly higher than those in NAA-treated roots. However, content of prosapogenin A was not significantly different among NAA or IBA level. Results provide that liquid culture of adventitious roots of D. nipponica have a potential for mass production of dioscin including prosapogenin A and prosapogenin C.

A New Early-Heading and High Forage Yielding Rye Variety "Egreen" (극조숙, 다수성 조사료 및 녹비용 호밀 신품종 "이그린")

  • Heo, Hwa-Young;Park, Hyoung-Ho;Hwang, Jong-Jin;Kim, Hong-Sik;Han, Ouk-Kyu;Park, Tae-Il;Seo, Jae-Hwan;Kim, Dea-wook;Kim, Su-Yong;Kim, Si-Ju;Park, Ki-Hun
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.620-624
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    • 2009
  • "Egreen" rye (Secale cereale L.) was developed by National Institute of Crop Science (NICS), RDA. It was released in 2007 because of its excellent earliness and yield potential. Crossing was made by an open pollination with 10 rye lines in 1995. Many lines from the open pollinated population were handled in a mass selection program in subsequent generations. Among them the line 'SR95POP-S1-116-1-1-2'was selected for earliness and promising agronomic appearance and placed in yield trial for two years from 2003 to 2004. In 2005, it was designated "Homil 34" and subjected to regional yield trials. Over 3 years, "Egreen" averaged 8.34 MT ha-1 of forage yield (based on dry matter) harvested at the heading date of check variety "Olhomil", which was superior to other varieties such as "Olhomil" and "Koolgrazer" with an increase of 8% and 10%, respectively. Heading date of "Egreen" was April 22, 3 days earlier than that of "Olhomil". It would be recommended as a winter crop forforage and green manure before planting either maize or rice in Korea.

Breeding of White Colored Cymbidium spp.'Wedding Day' for Cut Flower (절화특성이 우수한 백색 심비디움 '웨딩데이' 육성)

  • Yae Jin Kim;Pil Man Park;O Hyeon Kwon;Hye Ryun An;Hyun young Song;Kyung Ran Do;Pue Hee Park
    • Korean Journal of Plant Resources
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    • v.37 no.4
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    • pp.423-430
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    • 2024
  • Cymbidium (C.) spp. 'Wedding Day' was developed by the National Institute of Horticultural and Herbal Science, Rural Development Administration in 2021. This cultivar was derived from the artificial cross between C. 'Boksam Holiday' and C. 'Persicolor' in 2008. After the crossing, 112 seedlings were obtained through in vitro germination and transferred to the green house. Based on their vegetative and flowering characteristics, two lines were selected through the first selection. To confirm the stability and uniformity of the two lines, the first and second trials were conducted from 2017 to 2021. As a result, the final line with the code 'C0844-29' was selected as 'Wongyuo F1-78'. After evaluating consumer preference, the line was named as 'Wedding Day'. This hybrid is medium sized cultivar with long vase life of cut flowers and more than 11 white flowers per stalk. It has 63.2 cm of erect stalks which are suitable for cut flower. The leaves are 65.8 cm long and located lower than the flowers, providing appropriate shape for viewing the flowers. It starts flowering from late January. It has sufficient proliferation ability to enable mass proliferation for commercial use (Registration No. 9523).

Aesthetic Characteristics of Elegance Expressed on Fashion Since 1990s (1990년대 이후 패션에 나타난 엘레강스 스타일의 미적 특성)

  • Ko Hyunzin
    • Journal of the Korean Society of Clothing and Textiles
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    • v.29 no.3_4 s.141
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    • pp.403-413
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    • 2005
  • The purpose of this study is to interpret the elegant dressing visualized in modem fashion on the basis of the concept of elegance in dress and its aesthetic characteristics from the holistic viewpoint. Elegance in dress is based upon the idea of aristocratic taste cultivated by good breeding, considering from the documentary study. It is expressed visually through not only the carefully contrived dress but also a sort of aura of dressed body with skillful ease. The aesthetic values of elegance consist of luxury, nobility, refinement, femininity, harmony. To grasp the trends of elegant styles since 1990s, contents analysis of the articles related to elegance in Vogue has been done. As a result, they have been classified into four groups of elegant styles which are Classic, Elaborate Couture, Soft Minimalism, Kitsch Elegance. Classic Elegance and Elaborate Couture Elegance represent traditional ones with conservative viewpoint. Soft Minimalism Elegance is a modem version of elegance. According to postmodernism as a open system, even kitsch has been refined to keep accompany with elegance fur aristocratic taste of high class. As a result of this study, in fashion from the 16th century to the first half of 16th century, elegance has been one of the significant aesthetic categories, resulting from the absolute domination of taste of high society. However in the end of 20th century it seems to start to fade in fashion trends such as mass fashion, youth culture, casual fashion etc. Rather, it can be thought that elegance has been expressed as one of the aesthetic values in dress, by virtue of its value of high class as well as its conservative stability.

A synonymous mutation of uncoupling protein 2 (UCP2) gene is associated with growth performance, carcass characteristics and meat quality in rabbits

  • Liu, Wen-Chao;Lai, Song-Jia
    • Journal of Animal Science and Technology
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    • v.58 no.1
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    • pp.3.1-3.6
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    • 2016
  • Background: Uncoupling proteins 2 (UCP2) plays an important role in energy regulation, previous studies suggested that UCP2 is an excellent candidate gene for human obesity and growth-related traits in cattle and chicks. The current study was designed to detect the genetic variation of UCP2 gene, and to explore the association between polymorphism of UCP2 gene and growth, carcass and meat quality traits in rabbits. Results: A synonymous mutation in exon 1 and four variants in the first intron of the UCP2 gene were identified by using PCR-sequencing. The synonymous mutation c.72G>A was subsequently genotyped by MassArray system (Sequenom iPLEXassay) in 248 samples from three meat rabbit breeds (94 Ira rabbits, 83 Champagne rabbits, and 71 Tianfu black rabbits). Association analysis suggested that the individuals with AA and AG genotypes showed greater 70 d body weight (P < 0.05), 84 d body weight (P < 0.01), ADG from 28 to 84 days of age (P < 0.05), eviscerated weight (P < 0.01), semi-eviscerated weight (P < 0.01) and semi-eviscerated slaughter percentage (P < 0.05), respectively. Additionally, the individuals with AA and AG genotype had a lower pH value of longissimus muscle (P < 0.01) and hind leg muscle (P < 0.05) after slaughter 24 h. Conclusions: These findings indicated that UCP2 could be a candidate gene that associated with growth performance, body composition and meat quality in rabbits, and this would contribute to advancements in meat rabbit breeding practice.

Artificial Diet for Mass Rearing the Emma Field Cricket, Teleogryllus emma (Orthoptera: Gryllidae)

  • Kim, Nam-Jung;Hong, Seong-Jin;Seol, Kwang-Youl;Kim, Seong-Hyun;Ahn, Nan-Hee;Park, Hae-Chul;Lee, Young-Bo;Kim, Mi-Ae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.15 no.2
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    • pp.157-160
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    • 2007
  • Nymph of the emma field cricket, Teleogryllus emma, were reared on several types of artificial diets. The development period of nymphs were 55.4 days when only a single food, wheat bran, was provided, and it did not show a significant difference compared to the rearing results of the Danong diet and mixed diet. The supplying period of fish meal as the animal feed, the high emergence rates were obtained at 3rd instar with 90% and 4th instar with 100%. For the added amount test, when more than 40% of the diet was added, it confirmed that the insect weight increased. The characteristics of development according to each added amount of the vegetable food (dry bean-curd residue and corn powder) were investigated to minimize the dangers of the degeneration of diet when rearing with a single feed during the $1st{\sim}3rd$ instar period. First, as the added amount of bean-curd residue increased, nymphal development period became longer and the emergence rate became low. With corn powder as the single diet, all died before becoming adult. However, when corn powder was added up to 30%, no difference existed in the breeding results.

Evaluation for Concentration of Residual PCDD/Fs in Pig Feed (돼지사료 중의 다이옥신 잔류 평가)

  • Ahn, Yun-Gyong;Shin, Jeoung-Hwa
    • The Korean Journal of Community Living Science
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    • v.23 no.2
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    • pp.129-136
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    • 2012
  • Dioxins are chemical substances that are not properly decomposed degradation under natural photochemical, biological and chemical conditions in the environment, and bio-accumulate through the food chain, so pose a risk of causing adverse effects to human health and the environment. Ninety seven percent of human exposure to dioxins comes through meat, fish, dairy consumption and so on. Pork is the largest proportion meat aspect of daily intake in the Korean food. Pigs are three times more than Cattle in terms of breeding population and production amount in the world, and the amount is increasing every year. Therefore, it is necessary to monitor the exposure level of dioxins in pig's body. Also, it is required to evaluate the presence of these chemical substances in the pig's feed grain such as corn, soybeans, and tallow. The purpose of this study was to evaluate PCDD/Fs in pig's feed to search the origin of PCDD/Fs. Feed samples obtained were wheat from East Europe, corn from South America and America, soybean meal from Korea, America, South America and India and tallow from Korea. The preparation of samples was based on the EPA method 1613. Instrumental analysis was based on the use of high resolution gas chromatography coupled to high resolution mass spectrometry (HRGC/HRMS). The concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 1,2,3,7,8-pentachlorodibenzo-p-dioxin (PCDD), which are the most toxic potent dioxins, were not observed in any of the samples. However OCDD was detected in most of the samples. The TEQs in the feed samples were ranged from 0 pg TEQ/g to 0.09841 pg TEQ/g. Especially, the high level of TEQs was observed in the samples of wheat from East Europe and soybean meal from Korea.

Genome-wide association study of rice core set related selenium content

  • Choi, Buung;Lee, Sang Beom;Kim, Gyeong Jin;Kim, Kyu Won;Yoo, Ji Hyock;Oh, Kyeong Seok;Moon, Byeong Churl;Park, Yong Jin;Park, Sang Won
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.158-158
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    • 2017
  • The purpose of this study was to identify the candidate genes involved in selenium content in brown rice. Rice (Oryza sativa L.) was important crop including diverse functional substance such as carbohydrate, protein, lysine and tocopherol, mineral. Especially, selenium as nutritionally important minerals, it was known to activate the immune system, antioxidant effect and inhibition of carcinogenesis. Also recommended daily requirements of the United States and the United Kingdom were 55 to 90 ug for selenium. Therefore, selenium content in brown rice of core-set were analyzed by using ICP-MS (Inductively Coupled Plasma Mass Spectrometer) and GWAS (Genome Wide Association Study) was conducted to search for candidate genes in this study. The new natural variants identified through haplotyping analysis would be useful to develop new rice varieties with improved storage ability of the valuable mineral through the future molecular breeding.

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