Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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A synonymous mutation of uncoupling protein 2 (UCP2) gene is associated with growth performance, carcass characteristics and meat quality in rabbits

  • Liu, Wen-Chao (Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University) ;
  • Lai, Song-Jia (Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University)
  • Received : 2015.09.15
  • Accepted : 2016.01.05
  • Published : 2016.01.31
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Abstract

Background: Uncoupling proteins 2 (UCP2) plays an important role in energy regulation, previous studies suggested that UCP2 is an excellent candidate gene for human obesity and growth-related traits in cattle and chicks. The current study was designed to detect the genetic variation of UCP2 gene, and to explore the association between polymorphism of UCP2 gene and growth, carcass and meat quality traits in rabbits. Results: A synonymous mutation in exon 1 and four variants in the first intron of the UCP2 gene were identified by using PCR-sequencing. The synonymous mutation c.72G>A was subsequently genotyped by MassArray system (Sequenom iPLEXassay) in 248 samples from three meat rabbit breeds (94 Ira rabbits, 83 Champagne rabbits, and 71 Tianfu black rabbits). Association analysis suggested that the individuals with AA and AG genotypes showed greater 70 d body weight (P < 0.05), 84 d body weight (P < 0.01), ADG from 28 to 84 days of age (P < 0.05), eviscerated weight (P < 0.01), semi-eviscerated weight (P < 0.01) and semi-eviscerated slaughter percentage (P < 0.05), respectively. Additionally, the individuals with AA and AG genotype had a lower pH value of longissimus muscle (P < 0.01) and hind leg muscle (P < 0.05) after slaughter 24 h. Conclusions: These findings indicated that UCP2 could be a candidate gene that associated with growth performance, body composition and meat quality in rabbits, and this would contribute to advancements in meat rabbit breeding practice.

Keywords

References

  1. Schrauwen P, Hesselink M. UCP2 and UCP3 in muscle controlling body metabolism. J Exp Biol. 2002;205:2275-85.
  2. Echtay KS, Roussel D, St-Pierre J, Jekabsons MB, Cadenas S, Stuart JA. Superoxide activates mitochondrial uncoupling proteins. Nature. 2002;415:96-9. https://doi.org/10.1038/415096a
  3. Fleury C, Neverova M, Collins S, Raimbault S, Champigny O, Levi-Meyrueis C, et al. Uncoupling protein-2: a novel gene linked to obesity and hyperinsulinemia. Nat Genet. 1997;15:269-72. https://doi.org/10.1038/ng0397-269
  4. Boss O, Hagen T, Lowell BB. Uncoupling proteins 2 and 3: potential regulators of mitochondrial energy metabolism. Diabetes. 2000;49:143-56. https://doi.org/10.2337/diabetes.49.2.143
  5. Rossmeisl M, Syroy I, Baumruk F, Flachs P, Janovska P, Kopecky J. Decreased fatty acid synthesis due to mitochondrial uncoupling in adipose tissue. Faseb J. 2000;14:1793-800. https://doi.org/10.1096/fj.99-0965com
  6. Zhang C, Baffy G, Perret P, Krauss S, Peroni O, Grujic D, et al. Uncoupling protein-2 negatively regulates insulin secretion and is a major link between obesity, ${\beta}$ cell dysfunction, and type 2 diabetes. Cell. 2001;105:745-55. https://doi.org/10.1016/S0092-8674(01)00378-6
  7. Cassell PG, Neverova M, Janmohamed S, Uwakwe N, Qureshi A, McCarthy MI, et al. An uncoupling protein 2 gene variant is associated with a raised body mass index but not Type II diabetes. Diabetologia. 1999;42:688-92. https://doi.org/10.1007/s001250051216
  8. Yanovski JA, Diament AL, Sovik KN, Nguyen TT, Li H, Sebring NG, et al. Associations between uncoupling protein 2, body composition, and resting energy expenditure in lean and obese African American, white, and Asian children. Am J Clin Nutr. 2000;71:1405-20. https://doi.org/10.1093/ajcn/71.6.1405
  9. Lee YH, Kim W, Yu BC, Park BL, Kim LH, Shin HD. Association of the ins/del polymorphisms of uncoupling protein 2 (UCP2) with BMI in a Korean population. Biochem Bioph Res Co. 2008;371:767-71. https://doi.org/10.1016/j.bbrc.2008.04.144
  10. Sherman EL, Nkrumah JD, Murdoch BM, Li C, Wang Z, Fu A, et al. Polymorphisms and haplotypes in the bovine neuropeptide Y, growth hormone receptor, ghrelin, insulin-like growth factor 2, and uncoupling proteins 2 and 3 genes and their associations with measures of growth, performance, feed efficiency, and carcass merit in beef cattle. J Anim Sci. 2008;86:1-16. https://doi.org/10.2527/jas.2007-0687
  11. Ryu J, Kim Y, Kim C, Kim J, Lee C. Association of bovine carcass phenotypes with genes in an adaptive thermogenesis pathway. Mol Biol Rep. 2012;39:1441-5. https://doi.org/10.1007/s11033-011-0880-5
  12. Oh JD, Kong HS, Lee JH, Choi IS, Lee SJ, Lee SG, et al. Identification of novel SNPs with effect on economic traits in uncoupling protein gene of Korean native chicken. Asian-Aust J Anim Sci. 2006;19:1065-70. https://doi.org/10.5713/ajas.2006.1065
  13. Zhao J, Li H, Kong X, Tang Z. Identification of single nucleotide polymorphisms in avian uncoupling protein gene and their association with growth and body composition traits in broilers. Can J Anim Sci. 2006;86:345-50. https://doi.org/10.4141/A05-031
  14. Liu S, Wang SZ, Li ZH, Li H. Association of single nucleotide polymorphism of chicken uncoupling protein gene with muscle and fatness traits. J Anim Breed Genet. 2007;124:230-5. https://doi.org/10.1111/j.1439-0388.2007.00654.x
  15. Zhang GW, Wang HZ, Chen SY, Li ZC, Zhang WX, Lai SJ. A reduced incidence of digestive disorders in rabbits is associated with allelic diversity at the TLR4 locus. Vet Immunol Immunop. 2011;144:482-6. https://doi.org/10.1016/j.vetimm.2011.08.009
  16. Blasco A, Ouhayoun J. Harmonization of criteria and terminology in rabbit meat research. Revised proposal. World Rabbit Sci. 1993;4:93-9.
  17. Botstein D, White RL, Skolnick M, Davis RW. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet. 1980;32:314-31.
  18. Saltzman E, Roberts SB. The role of energy expenditure in energy regulation: findings from a decade of research. Nutr Rev. 1995;53:209-20.
  19. Adams SH. Uncoupling protein homologs: emerging views of physiological function. J Nutr. 2000;130:711-4. https://doi.org/10.1093/jn/130.4.711
  20. Gou P, Comaposada J, Arnau J. Meat pH and meat fibre direction effects on moisture diffusivity in salted ham muscles dried at $5^{\circ}C$. Meat Sci. 2002;61:25-31. https://doi.org/10.1016/S0309-1740(01)00158-9
  21. Guerrero L, Gou P, Arnau J. The influence of meat pH on mechanical and sensory textural properties of dry-cured ham. Meat Sci. 1999;52:267-73. https://doi.org/10.1016/S0309-1740(98)00175-2
  22. Li L, Skorpen F, Egeberg K, Jorgensen IH, Grill V. Uncoupling protein-2 participates in cellular defense against oxidative stress in clonal ${\beta}$-cells. Biochem Bioph Res Co. 2001;282:273-7. https://doi.org/10.1006/bbrc.2001.4577
  23. Yang Y, Zhang GW, Chen SY, Peng J, Lai SJ. Polymorphism of NLRP3 gene and association with susceptibility to digestive disorders in rabbit. Asian-Aust J Anim Sci. 2013;26:455-62. https://doi.org/10.5713/ajas.2012.12522
  24. Zhang WX, Zhang GW, Peng J, Zhang JL, Yang Y, Lai SJ. A synonymous mutation in NOD2 gene was significantly associated with non-specific digestive disorder in rabbit. Gene. 2013;516:193-7. https://doi.org/10.1016/j.gene.2012.12.091

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