• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2010년도 추계학술대회
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• pp.343-344
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• 2010

마커리스 시스템의 경우 2차원 영상에서 깊이 값을 추정하기 위해서는 스테레오 비젼과 같이 고가의 장비를 통해 깊이 값을 추정하였다. 이에 단안 영상에서 깊이 값을 추정하여 객체를 증강하기 위해 소실점을 추출하고 상대적 깊이 값을 추정한다. 객체 증강에 있어 향상된 몰입감을 얻기 위해서는 가상의 객체들이 거리에 따라 서로 다른 크기로 그려져야 한다. 본 논문에서는 획득한 영상에서 소실점을 생성하고 깊이정보를 이용하여 증강된 객체를 서로 다른 크기로 증강하여 객체간 상호 몰입감을 향상시켰다.

• 대한건축학회논문집:계획계
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• 제34권8호
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• pp.87-94
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• 2018

Hwaseong Fortress was designated as a World Heritage Site by UNESCO in 1997 in recognition of its architectural, cultural, and historical significance. In order to provide a compelling and active experience to the visitors of Hwaseong Fortress more effectively and intuitively, an augmented reality (AR) mobile application is developed. The main objective of this paper is to describe the implementation of AR technology in the developed application; and most importantly, how the application is designed for the visitors to actively and intuitively experience the various architectural and historical contents of Hwaseong. The tour routes and their point of interests were selected carefully after deliberation of their contents. To maximize the user interaction with the application and the architectural contents of Hwaseong, not only the conventional AR technology (marker method), but also the markerless AR method is adopted in the developed application. It is expected that visitors of Hwaseong Fortress enjoy the tour, while learning the architectural, historical, and cultural contents through the active and intuitive interaction with the developed AR mobile application.

• Journal of Microbiology and Biotechnology
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• 제31권12호
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• pp.1722-1731
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• 2021

The genus Streptomyces is intensively studied due to its excellent ability to produce secondary metabolites with diverse bioactivities. In particular, adequate precursors of secondary metabolites as well as sophisticated post modification systems make some high-yield industrial strains of Streptomyces the promising chassis for the heterologous production of natural products. However, lack of efficient genetic tools for the manipulation of industrial strains, especially the episomal vector independent tools suitable for large DNA fragment deletion, makes it difficult to remold the metabolic pathways and streamline the genomes in these strains. In this respect, we developed an efficient deletion system independent of the episomal vector for large DNA fragment deletion. Based on this system, four large segments of DNA, ranging in length from 10 kb to 200 kb, were knocked out successfully from three industrial Streptomyces strains without any marker left. Notably, compared to the classical deletion system used in Streptomyces, this deletion system takes about 25% less time in our cases. This work provides a very effective tool for further genetic engineering of the industrial Streptomyces.

• Journal of Microbiology and Biotechnology
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• 제24권5호
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• pp.619-628
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• 2014

To identify lipase LipA (PFL_0617) from Pseudomonas protegens Pf-5, a lipA deletion mutant (Pf0617) and a complementary strain (Pf0617lipA) were constructed, and their effects on the lipase production were examined. Pf0617 remarkably decreased its whole-cell lipase activity, whereas Pf0617lipA made its whole-cell lipase activity not only restore to wild-type level but also get a further increment. However, the deletion and overexpression of lipA did not affect the extracellular lipase activity. In addition, the unbroken whole cells of these strains were able to catalyze the hydrolysis of membrane-permeable p-nitrophenyl esters, but could not hydrolyze the membrane-impermeable olive oil. These results confirmed that LipA was an intracellular lipase and Pf-5 could also be used as a natural whole-cell biocatalyst. To evaluate the potential of Pf-5 as a whole-cell biocatalyst and separately characterize the whole-cell LipA, the properties of the whole-cell lipases from Pf-5 and Top10lipA were characterized. The results demonstrated that both Pf-5 and Top10lipA exhibited high tolerance to alkaline condition, high temperature, heavy metal ions, surfactants, and organic solvents. Taken together, lipA can realize functional expression in E. coli Top10, and Pf-5 and Top10lipA as whole-cell biocatalysts may have enormous potential in applications.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.45-53
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• 2010

We have developed an efficient and precise method for genome manipulations in Bacillus subtilis that allows rapid alteration of a gene sequence or multiple gene sequences without altering the chromosome in any other way. In our approach, the Escherichia coli toxin gene mazF, which was used as a counter-selectable marker, was placed under the control of a xylose-inducible expression system and associated with an antibiotic resistance gene to create a "mazF-cassette". A polymerase chain reaction (PCR)-generated fragment, consisting of two homology regions joined to the mazF-cassette, was integrated into the chromosome at the target locus by homologous recombination, using positive selection for antibiotic resistance. Then, the excision of the mazF-cassette from the chromosome by a single-crossover event between two short directly repeated (DR) sequences, included in the design of the PCR products, was achieved by counter-selection of mazF. We used this method efficiently and precisely to deliver a point mutation, to inactivate a specific gene, to delete a large genomic region, and to generate the in-frame deletion with minimal polar effects in the same background.

• 한국멀티미디어학회논문지
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• 제15권8호
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• pp.1004-1016
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• 2012

본 논문에서는 제품에 삽입된 QR코드를 사용해 제품의 상세한 정보를 제공해주는 모바일 증강현실 시스템을 제안한다. 본 시스템에서는 마커기반의 자세 추정 기법과 마커리스 기반의 기법을 함께 적용하여 보다 강인한 카메라 자세 추정을 수행한다. QR코드가 근거리에 있거나, QR코드 전체가 영상 내에 들어올 경우에는 QR코드 내/외부의 사각형 집합을 추적하여 카메라 자세를 추정한다. 하지만, 영상 내에서 마커가 사라지거나, 모바일 기기가 QR코드 식별이 힘든 먼 거리에 위치할 경우 프레임간의 호모그래피에 기반을 둔 카메라 자세 보정을 수행한다. 또한 제안된 시스템은 증강현실 콘텐츠 제작을 위해서 메타데이터를 사용함으로서 사용자가 프로그램 수정 없이 메타데이터 파일을 작성하는 것만으로 다양한 시나리오의 콘텐츠를 제작 및 수정하는 것이 가능하다. 특히 증강현실 콘텐츠 가시화를 위한 메타데이터와 미디어 파일을 온라인 서버를 통해 항상 최신의 상태로 전송받을 수 있기 때문에 프로그램 업데이트와 같은 불필요한 작업을 최소화할 수 있다.

• 대한견주관절학회:학술대회논문집
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• 대한견주관절학회 2009년도 제17차 학술대회
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• pp.174-174
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• 2009

The acromioclavicular-hook-plate is one of the surgical treatments for distal clavicle fracture and traumatic acromioclavicular (AC) joint dislocation. Although this procedure can obtain rigid and accurate anatomical reduction of the AC joint, secondary widening of the hook-hole in the acromion is often seen during postoperative follow-up. This complication is owing to the high-degree of mobility of the AC joint. Therefore, it is important to evaluate the effect on these complications due to the position of the hook-hole. The purpose of the present study is to investigate three-dimensionally the effect due to the position of the hook-hole during arm abduction motion. We studied in vivo and three-dimensional kinematics of the normal shoulder joint with use of a markerless bone-registration technique. Magnetic resonance images of 14 shoulders of 7 healthy volunteers were acquired in 7 positions between $0^{\circ}$ and $180^{\circ}$ of abduction. We created three-dimensional computer models of the bones and the acromioclavicular-hook-plate. Based on the three-dimensional kinematics data, we simulated the widening of the hook-hole each different positioning of the hook-hole. The widths of the hook-holes almost linearly increased. And these widths significantly increased, when we put the hook-hole on the acromion from AC joint to 20 mm and 25 mm posterior position.

• 대한견주관절학회:학술대회논문집
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• 대한견주관절학회 2009년도 제17차 학술대회
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• pp.43-43
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• 2009

Glenohumeral ligaments play an important role in stabilizing the shoulder. However, it is impossible to know how they function in vivo during shoulder motion. To help elucidate this stabilizing role, we conducted in vivo three-dimensional kinematics of the normal shoulder joint using a markerless bone-registration technique. Magnetic resonance images of 14 shoulder joints of 7 healthy volunteers were acquired for 7 isometric abduction positions between $0^{\circ}$ and $180^{\circ}$. We then calculated three-dimensional shortest paths between the origin and insertion of each ligament based on anatomical study in each abduction position. At $0^{\circ}$ of abduction, the posterior band of the coracohumeral ligament displayed the maximum length. At $30^{\circ}$ of abduction, the superior glenohumeral ligament displayed the maximum length. At $60^{\circ}$ of abduction, the anterior band of the coracohumeral ligament and the middle glenohumeral ligament displayed the maximum length. At $120^{\circ}$ of abduction, the anterior band of the inferior glenohumeral ligament displayed the maximum length. We think that the maximum length of these results is an important influence on the function of the soft tissue stabilizer.

• 한국게임학회 논문지
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• 제12권4호
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• pp.45-51
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• 2012

상호작용 증강현실 인터페이스는 실세계에 가상 정보를 혼합하여 사용자의 인식을 증강시킬 수 있으며 사용자에게 실세계에서 이용되는 오브제를 가상 게임에 그대로 제공함으로써 현실과 가상 환경을 자연스럽게 연계할 수 있다. 우리는 실생활과 가상 게임 환경과의 이음새 없는 환경을 위해 TCG(Trading Card Game; 이하 TCG로 약칭) 게임에 실제 카드를 적용하는 방안을 연구하였다. 스마트 폰 카메라를 통해 인식된 카드를 가상의 TCG 게임 인터페이스로 사용한다. 기존 연구에 비해 보다 빠르고 스케일, 방향, 포즈, 회전에 무관하게 실 카드를 인식하기 위해 2 단계 이미지 키포인트 추출/매칭 방법(Two Phases Image eyPoint Extraction/Matching Method)을 스마트폰 환경에서 개발하고 이것을 가상 TCG 게임 시스템에 실험적으로 적용하였다. 이와 같은 창의적인 시도는 현실과 가상 환경을 자연스럽게 연결하여 게임에의 몰입감과 현실감을 배가시킬 것이다.

• 한국HCI학회논문지
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• 제10권2호
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• pp.45-55
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• 2015

최근 증강현실 분야에서 특징점 인식 및 추적 기술은 비마커 기반의 증강 현실 서비스 구현에 중요한 역할을 담당하고 있다. 특징점 인식 및 추적 기술은 오래 전부터 컴퓨터 비전 등 여러 분야의 많은 연구자들에 의해 심도 있게 연구되어 왔으며, 특히 최근 급성장하고 있는 모바일 관련 시스템에 적용하기 위해 모바일 임베디드 환경에 접목 가능한 특징점 기반의 다양한 인식 및 추적 기술들이 소개되고 있다. 따라서 본 논문에서는 널리 활용되고 있는 특징점 기반의 매칭 및 추적의 다양한 핵심 요소 기술 (특징점 추출, 특징점 기술, 특징점 매칭 및 추적)에 대한 최신 동향을 분석하고, 본 한국과학기술연구원 연구팀이 수행한 모바일 증강현실 서비스 관련 사례 연구인 관광 지도 인식 및 추적 연구를 소개하고자 한다.