• Parasites, Hosts and Diseases
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• 제51권3호
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• pp.369-373
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• 2013

The present study was performed to trace the decisive evidence for mixed infection of 2 Myxobolus species, M. episquamalis and Myxobolus sp., in the gray mullet, Mugil cephalus, from Korean waters. Mullets with whitish cyst-like plasmodia on their scales were collected near a sewage plant in Yeosu, southern part of Korea, in 2009. The cysts were mainly located on scales and also found in the intestine. The spores from scales were oval in a frontal view, tapering anteriorly to a blunt apex, and measured $7.2{\mu}m$ (5.8-8.0) in length and $5.3{\mu}m$ (4.7-6.1) in width. Two polar capsules were pyriform and extended over the anterior half of the spore, measuring $3.5{\mu}m$ (2.3-4.8) in length and $2.0{\mu}m$ (1.5-2.2) in width. In contrast, the spores from the intestine were ellipsoidal, $10.4{\mu}m$ (9.0-11.9) in length and $8.4{\mu}m$ (7.3-10.1) in width. The polar capsules were pyriform but did not extend over the anterior half of the spore, $3.7{\mu}m$ (2.5-4.5) in length and $2.2{\mu}m$ (1.8-2.9) in width. The nucleotide sequences of the 18S rDNA gene of the 2 myxosporean spores from scales and intestine showed 88.1% identity to each other and 100% identity with M. episquamalis and 94.5% identity with M. spinacurvatura from mullet, respectively. By the above findings, it is first confirmed that mullets from the Korean water are infected with 2 myxosporean species, M. episquamalis and Myxobolus sp.

• 수산해양기술연구
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• 제53권4호
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• pp.413-422
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• 2017

The objective of this study was to prove possible effects of a habitable place for fish around semi-submersible drilling rig which measured underwater radiated noise, irradiance distribution of rig's light and conducted experimental fishing in Malaysia territory and observed for distribution of fishes around the rig by ROV during drilling operation in the East Sea of Korea. As a result underwater radiated noise was $130dB/{\mu}Pa$ from 156.25 to 187.50 Hz, irradiance distribution of light was between 21.5 and 43.1 lx. Despite this result, underwater radiated noise was not a threat to fish around rig and low irradiance distribution of light also played a role in the luring influence of the fishes at night. Fish showed up in the beginning of drilling operation and at the end of drilling operation. However, there are few fish in the midst of drilling operation. The main reason for this result was the destruction of the habitable place due to blow out mud and broken pieces of ground formation in drilling hole. The results are expected to contribute to reasonable fishery management around rig.

• 한국식품과학회지
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• 제43권3호
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• pp.381-386
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• 2011

실험물질은 두피건강 및 발모에 유용한 소재를 혼합하여 열수 추출한 후 유산균에 발효시킨 2차 발효물질로서 고기능성이 예상되는 물질이다. C57BL/6 마우스의 등을 제모하고 실험물질을 처리하였을 때 암 수 모두에서 양성대조군인 minoxidil과 비교하였을 때 동등이상의 우수한 발모효과가 있음을 확인하였다. 특히 수컷의 경우 200 mg/kg 이하의 농도에서 우수한 효과가 관찰되었고 암컷에서는 고용량인 500 mg/kg까지 현저한 발모효과가 관찰되어 성별 처치 용량에 차이가 있는 것으로 사료되었다. 또한 양성대조군 minoxidil이 신속한 발모효과를 보이는 반면 털의 긁기가 감소하는 현상이 관찰되었으나 실험물질을 처리한 군에서는 제모전과 동등이상의 굵기를 유지하였다. 이러한 발모효과를 확인하기위해 모발성장관련 유전자 발현을 분석한 결과 실험물질이 KGF, VEGF 등의 모발성장촉진인자의 발현을 증가시키는 반면 탈모를 유도하는 $TGF{\beta}1$의 발현에는 반응을 하지 않아 유용한 발모제제로서의 가능성이 입증되었다. 더불어 실험물질에 의한 NO 분비가 두피의 혈류개선을 유도하여 탈모억제효능을 나타낼 것으로 사료되어 발효생약추출물인 MBN이 모발성장 및 탈모예방제제로의 개발이 가능 할 것으로 기대된다.

• 한국해양공학회지
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• 제26권5호
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• pp.5-10
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• 2012

In this study, carbon steel (A53) is used as the material for the pipes in a marine plant and ship industry. Welds are necessary to join the carbon steel, and the effect of this welding on the properties of the carbon steel has been studied by many researchers. In this study, the dynamic behavior of welded carbon steel was studied using an acoustic emission (AE) technique, which is a nondestructive test. There are numerous AE parameters that can be used to analyze the damage behavior of carbon steel by external loading. The AE parameters of energy, cumulative count, amplitude, and AE event were used, and each parameter was differentiated according to the degree of damage to the carbon steel. The energy showed a high level at the elastic range of the load curve, while the amplitude had the highest value at the hardening region. The cumulative count showed a growth tendency similar to the loading curve. In addition, an ultrasonic technique and hardness test were applied to evaluate the mechanical properties according to the base zone, HAZ region, and weld zone of the weld specimen. The velocity and attenuation ratio showed little change between zones, and an evaluation of the ultrasonic waves on each zone of the specimen was found to be a useful method to clarify the mechanical properties of the carbon steel.

• 한국해양공학회지
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• 제31권2호
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• pp.130-140
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• 2017

Typically, a submerged breakwater is one of the good scene-friendly coastal structures used to reduce wave energy and coastal erosion. However, sometimes, a submerged breakwater also has a negative aspect in that a strong rip current occurring around an open inlet due to a difference in mean water levels on the front and rear sides of the structure leads to scouring. Such scouring has a bad effect on its stability. In order to eliminate this kind of demerit, this study investigated four new types of submerged breakwaters with drainage channels. First, hydraulic experiments were performed the typical and new structures. Then, the wave height and mean water level distributions around the structures were examined using the experimental results. Finally, it was revealed that the new type of submerged breakwater could efficiently reduce the mean water level on its rear side. In particular, in the case of new-type submerged breakwater 2, an average reduction efficiency of 71.2% for the difference between the mean water levels at the front and rear sides was shown in comparison with the typical one.

• 대한조선학회논문집
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• 제47권5호
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• pp.733-742
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• 2010

In this study, optimization was performed to improve the conventional liquefaction process of offshore plants, such as a LNG-FPSO(Liquefied Natural Gas-Floating, Production, Storage, and Offloading unit) by maximizing the energy efficiency of the process. The major equipments of the liquefaction process are compressors, expanders, and heat exchangers. These are connected by stream which has some thermodynamic properties, such as the temperature, pressure, enthalpy or specific volume, and entropy. For this, a process design problem for the liquefaction process of offshore plants was mathematically formulated as an optimization problem. The minimization of the total energy requirement of the liquefaction process was used as an objective function. Governing equations and other equations derived from thermodynamic laws acted as constraints. To solve this problem, the sequential quadratic programming(SQP) method was used. To evaluate the proposed method in this study, it was applied to the natural gas liquefaction process of the LNG-FPSO. The result showed that the proposed method could present the improved liquefaction process minimizing the total energy requirement as compared to conventional process.

• 대한조선학회논문집
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• 제56권4호
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• pp.308-315
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• 2019

In this study, numerical simulations of slamming impact pressure acting on the flat plate and wedge type models using the cartesian-grid system and Modified Marker-Density Method (MMD method) were performed and the results were examined. The flat plate and wedge type models were selected as target objects, the turbulence characteristics were considered by applying the Sub-Grid Scale (SGS) turbulence model. Through this study, how the pressure acting on the target objects according to the incident angle influences the slamming impact pressure was examined and the results were compared with the flow characteristics of other experimental results. Also, the degree of slamming impact pressure is evaluated with respect to the cartesian-grid system and MMD method, which is easy to use and has a high degree of calculation for free surface.

• 한국해양공학회지
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• 제35권3호
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• pp.173-182
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• 2021

The maneuvering performance of a ship on the actual sea is very different from that in calm water due to wave-induced motion. Enhancement of a ship's maneuverability in waves at the design stage is an important way to ensure that the ship navigates safely. This paper focuses on the maneuvering prediction of a Russian trawler in wind and irregular waves. First, a unified seakeeping and maneuvering analysis of a Russian trawler is proposed. The hydrodynamic forces acting on the hull in calm water were estimated using empirical formulas based on a database containing information on several fishing vessels. A simulation of the standard maneuvering of the Russian trawler was conducted in calm water, which was checked using the International Maritime Organization (IMO) standards for ship maneuvering. Second, a unified model of seakeeping and maneuvering that considers the effect of wind and waves is proposed. The wave forces were estimated by a three-dimensional (3D) panel program (ANSYS-AQWA) and used as a database when simulating the ship maneuvering in wind and irregular waves. The wind forces and moments acting on the Russian trawler are estimated using empirical formulas based on a database of wind-tunnel test results. Third, standard maneuvering of a Russian trawler was conducted in various directions under wind and irregular wave conditions. Finally, the influence of wind and wave directions on the drifting distance and drifting angle of the ship as it turns in a circle was found. North wind has a dominant influence on the turning trajectory of the trawler.

• 생태와환경
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• 제53권4호
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• pp.408-426
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• 2020

This study aims to establish the national strategy for biodiversity conservation by analyzing the current status of ecodiversity as the foundation of biodiversity conservation. Furthermore, this study has another purpose of preparing the measures for conservation and restoration of biodiversity. Ecodiversity was discussed as the basis for conserving biodiversity. Five climate zones and 14 climatic regions, eight plant geographic regions, three massifs and major geologic series, horizontal and vertical topographic conditions, 16 ecoregions, major ecosystems including forest, river and streams, wetlands, coast and marine, agriculture, and urban esosystems, and land use types were discussed as the element of the ecodiversity. In terms of biodiversity conservation, the actual conditions of each ecological unit were reviewed and measures were proposed to reduce biodiversity loss. Destruction and fragmentation of habitat, poor ecosystem management due to socioeconomic changes, the effects of exotic species and chemicals, and climate change were discussed as the major factors causing biodiversity loss. Systematic monitoring based on scientific principles and ecological restoration based on those monitoring results were recommended as measures for biodiversity conservation.

• Journal of Microbiology and Biotechnology
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• 제31권12호
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• pp.1624-1631
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• 2021

Prodigiosin as a high-valued compound, which is a microbial secondary metabolite, has the potential for antioxidant and anticancer effects. However, the large-scale production of functionally active Hahella chejuensis-derived prodigiosin by fermentation in a cost-effective manner has yet to be achieved. In the present study, we established carbon source-optimized medium conditions, as well as a procedure for producing prodigiosin by fermentation by culturing H. chejuensis using 10 L and 200 L bioreactors. Our results showed that prodigiosin productivity using 250 ml flasks was higher in the presence of glucose than other carbon sources, including mannose, sucrose, galactose, and fructose, and could be scaled up to 10 L and 200 L batches. Productivity in the glucose (2.5 g/l) culture while maintaining the medium at pH 6.89 during 10 days of cultivation in the 200 L bioreactor was measured and increased more than productivity in the basal culture medium in the absence of glucose. Prodigiosin production from 10 L and 200 L fermentation cultures of H. chejuensis was confirmed by high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) analyses for more accurate identification. Finally, the anticancer activity of crude extracted prodigiosin against human cancerous leukemia THP-1 cells was evaluated and confirmed at various concentrations. Conclusively, we demonstrate that culture conditions for H. chejuensis using a bioreactor with various parameters and ethanol-based extraction procedures were optimized to mass-produce the marine bacterium-derived high purity prodigiosin associated with anti-cancer activity.