• Journal of Community Nutrition
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• 제5권2호
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• pp.83-92
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• 2003

Several self-administered dietary assessment questionnaires have recently been developed, validated, and used in nutritional epidemiological and clinical studies in Japan. This article describes recent evidence on development and validation of them. After extensive search of published articles both in English and Japanese languages, we identified 25 articles on 13 questionnaires of which validation studies have existed. Number of foods/menus assessed varied from 31 to 169 according to questionnaires. Eleven questionnaires were food frequency type, either with fixed portion size or semiquantitative, and two diet history types. All the 13 questionnaires were validated against intakes assessed with dietary record or 24-hour recall, and only two with biomarkers. Number of subjects used in the studies was between 23 and 350. All the studies used adult subjects. In the studies with dietary record or recall, the correlation coefficient for or orgy intake was between 0.22 and 0.65 (median = 0.44). Median correlation coefficient for nutrients was between 0.21 and 0.61. In the studies with biomarkers, serum marine-origin n-3 polyunsaturated fatty acids and carotenes, and urinary potassium seemed useful biomarkers. In conclusion, recent progress of this field in Japan is remarkable. But more research is needed for validation studies with biomarkers, and the development and validation of questionnaires for children and elderly subjects. (J Community Nutrition 5(2) : 83∼92,2003)

• Journal of Microbiology and Biotechnology
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• 제21권10호
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• pp.1026-1035
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• 2011

Vibrio parahaemolyticus, which owes its origin to the marine environment, is considered as one of the most common causes of infectious diarrhea worldwide. The present study investigated the pathogenicity of V. parahaemolyticus against the model organism, Caenorhabditis elegans. Infection in the host was localized with GFP-tagged V. parahaemolyticus using confocal laser scanning microscopy. The times required for causing infection, bacterial load in intestine, chemotactic response, and alteration in pharyngeal pumping were analyzed in the host system. In addition, the regulation of innate immune-related genes, lys-7, clec- 60, and clec-87, was analyzed using real-time PCR. The role of immune-responsible pmk-1 was studied using mutant strains. The pathogenicity of environmental strain CM2 isolated from the Gulf of Mannar, India was compared with that of a reference strain obtained from ATCC. The pathogen infected animals appeared to ward off infection by up-regulating candidate antimicrobial genes for a few hours after the exposure, before succumbing to the pathogen. For the first time, the pathogenicity of V. parahaemolyticus at both the physiological and molecular levels has been studied in detail using the model organism C. elegans.

• 대한환경공학회지
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• 제34권2호
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• pp.97-102
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• 2012

본 연구에서는 댐 하류에 있어서 Stenopsyche marmorata의 먹이원과 더불어 필수 지방산의 변화를 조사했다. 조사결과, 수중 POM의 기원은 규조류, 녹조류 같은 미세 조류 유래 유기물과 세균 유래 유기물이 큰 비중을 차지하고 있었다. 또한, S. marmorata의 지방산 검출에 의하면 규조류, 녹조류 및 세균이 주 먹이원으로 이용되고 있었다. S. marmorata의 ${\omega}3$ 필수 지방산은 ${\omega}6$보다 높게 검출되었으며, 전 조사 지점간에서 S. marmorata의 필수 지방산비는 약 7을 나타내고 있었다.

• 한국환경과학회지
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• 제13권9호
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• pp.819-826
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• 2004

In order to elucidate the polycyclic aromatic hydrocarbon concentration and its origin in arctic area, four arctic brown algae (Laminaria saccharina, L. digita, Alaria esculenta, Desmarestia aculeata), one marine invertebrate (Echinoidea) and sediments were collected from Kongsfjorden in Spitsbergen from the late July to early August, 2003. In case of macroalgae, the young blade part above growth point and the old stipes and blades beneath growth point were separated and analyzed for polycyclic aromatic hydrocarbons (PAHs) in an attempt to check the mechanism of uptake in macroalgae to accumulate PAH. There was no difference in PAH concentrations between sampling sites (Stations B and C), species, and blades beneath and above growth point. PAH concentrations in all samples collected in this study were relatively higher than those reported in other areas of arctic. Especially, station C, which is known as an unpolluted area, showed 10 times higher PAH concentration (8,765 ng/g) in sediment than station A (694 ng/g) around harbor. In addition high PAH concentration, station C had very higher proportion of methylated PAH to parent PAH in sediment than station A. Source analysis using PAH isomer pair ratios as indicators showed that Kongsfjorden area seemed to be relatively contaminated with PAH derived from direct petroleum input.

• 한국수산과학회지
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• 제46권5호
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• pp.594-597
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• 2013

Two leptocephali [total length (TL) 68.2-80.5 mm] from the family Ophichthidae, collected from the waters south of Jeju Island, Korea, were identified using morphology. The two ophichthid leptocephali were identified as belonging to the genus Ophichthus based on morphological characters: 131-132 myomeres; nine gut swellings; dorsal fin origin above the middle of the body; five postanal pigments present from the anus to the caudal margin. Ultimately, both leptocephali were identified as Ophichthus asakusae, as the total number of myomeres was consistent with those of adult O. asakusae. Here, we first describe the morphological characteristics of the O. asakusae leptocephalus, and suggest diagnostic characters for leptocephali belonging to the genus Ophichthus.

• Journal of Microbiology and Biotechnology
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• 제30권5호
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• pp.633-641
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• 2020

Microbial rhodopsins are a superfamily of photoactive membrane proteins with the covalently bound retinal cofactor. Isomerization of the retinal chromophore upon absorption of a photon triggers conformational changes of the protein to function as ion pumps or sensors. After the discovery of proteorhodopsin in an uncultivated γ-proteobacterium, light-activated proton pumps have been widely detected among marine bacteria and, together with chlorophyll-based photosynthesis, are considered as an important axis responsible for primary production in the biosphere. Rhodopsins and related proteins show a high level of phylogenetic diversity; we focus on a specific class of bacterial rhodopsins containing the '3 omega motif.' This motif forms a stack of three non-consecutive aromatic amino acids that correlates with the B-C loop orientation and is shared among the phylogenetically close ion pumps such as the NDQ motif-containing sodium-pumping rhodopsin, the NTQ motif-containing chloride-pumping rhodopsin, and some proton-pumping rhodopsins including xanthorhodopsin. Here, we reviewed the recent research progress on these 'omega rhodopsins,' and speculated on their evolutionary origin of functional diversity.

• 생명과학회지
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• 제9권1호
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• pp.99-105
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• 1999

Bacillus sp. RH-5로 배양한 배양액을 silica gel column (8.0cmX100cm)chromatography를 행하여 chloroform : methanol = 90 : 10인 항산화 분획층을 얻어 TLC 법에 의해 활성이 있는 물질을 분리하여 thiocyanate method로 활성을 측정하여 본 결과 Vit. E($10^{-2}$ mol)보다 좋았으며 BHA, BHT와는 비슷한 결과를 나타내었다. 한편 $5_\muM$ $CuSO_4$ 촉매 사람 Low Density Lipoprotein(LDL)의 산화에 항산화 활성이 알려진 Fraction 3의 band 4를 각각 100 및 200$\mu$g/$m\ell$ 농도로 $37^{\circ}C$에서 18시간 배양한 결과 LDL의 산화에 대한 억제효과가 좋았다. Fraction 3의 band 4를 HPLC로 정제한 후 IR. NMR 및 GC/MASS.로 확인한 결과 5-hydroxyndole로 확인되었다.

• Journal of Korean Society for Atmospheric Environment
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• 제22권E1호
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• pp.9-18
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• 2006

Aerosol hygroscopic properties were measured by a tandem differential mobility analyzer (TDMA) system during the Aerosol Characterization Experiment (ACE)-Asia campaign from 31 March to 1 May 2001. Two high flow differential mobility analyzers (DMAs) were used to maximize the count rate on board the Center for Interdisciplinary Remotely Piloted Aircraft (CIRPAS) Twin Otter aircraft. Hygroscopic growth factor distributions of particles having initial dry nanoparticle diameters of 0.040, 0.059, 0.086, 0.126, 0.186, 0.273, 0.400, and $0.586{\mu}m$ were measured during 19 research flights. Data collected during 12 of those flights were used to investigate aerosol mixing state and the influence of aerosol source region on size-resolved hygroscopicity. The uniformity in size-resolved hygroscopicity was quantified to facilitate comparison between measurements made in different air masses. Hygroscopic growth factors are strongly dependent on source region and sizes. Mean hygroscopic growth factors were observed to be greatest when the air mass origin was from the south. The mean growth factors for continental sources decreased with initial size from 1.47 to 1.27 for $0.040{\mu}m\;and\;0.586{\mu}m$, but increased with initial size from 1.44 to 1.8 for $0.040{\mu}m\;and\;0.400{\mu}m$ dry diameters for marine sources.

• 한국산업융합학회 논문집
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• 제23권4_1호
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• pp.531-539
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• 2020

When injection mold is repeatedly used for mass production, fatigue phenomenon due to cyclic stress may occur. The surface and interior of structure might be damaged due to cyclic stress or strain. The objective of this study was to analyze failure of SCM435 high-tension bolts connecting upper and lower parts of a three-stage injection molding machine. These bolts have to undergo an accurate heat treatment to prevent the formation of chromium carbide and the action of dynamic stresses. Bolts were fractured by cyclic bending stress in the observation of ratchet marks and beach marks. Damaged specimen showed an acicular microstructure. Impurity was observed. Chromium carbide was observed near the crack origin. Both shape parameters of the Vickers hardness were similar. However, the scale parameter of the damaged specimen was about 20% smaller than that of the as-received specimen. Much degradation occurred in the damaged specimen. Bolts should undergo an accurate heat treatment to prevent the formation of chromium carbide. They must prevent the action of dynamic stresses. Bolts need accurate tightening and accuracy of heat treatment and screws need compression residual stress due to peening.

• Fisheries and Aquatic Sciences
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• 제9권4호
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• pp.146-152
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• 2006

Systemic infections of maricultured fishes by Megalocytivirus species have occurred over a broad area in South Korea, causing extensive economic loss. We developed digoxigenin-labeled nucleic acid probes against the 230-bp ATPase and 311-bp major capsid protein (MCP) of rock bream Oplegnathus fasciatus iridovirus (RBIV) using polymerase chain reaction, and an in situ hybridization (ISH) method to detect Megalocytivirus in formalin-fixed tissues of mariculture species (rock bream, sea bass, and olive flounder). ISH-positive cells were abundant in the hematopoietic and connective tissues of various organs, while brain tissue showed little or no signal. The ISH procedure can become an important diagnostic tool in complement with histopathological methods, and advances epidemiological studies on the origin and distribution of Megalocytivirus in mariculture.