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Determination of Free 4-hydroxyproline with Dansylchloride by HPLC in Human Urine (소변 중 4-hydroxyproline 분석에 관한 연구)

  • Lee, Keou-Weon;Cho, Young-Bong;Lee, Kyung-Jong
    • Journal of Preventive Medicine and Public Health
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    • v.35 no.4
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    • pp.282-286
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    • 2002
  • Objectives : The level of 4-hydroxyproline (4-Hyp) in human urine was measured using high performance liquid chromatography (HPLC) with a fluorescence detector. This method is useful for medical examinations and investigating the radicals induced by physical, chemical, mental stresses. This method is superior to many published several methods in terms of its low cost and ability to analyze many samples. Methods : The urine from workers in a tire manufacturing company (22 male pre- and post-shift workers) and 18 office-workers as controls were analyzed. Data concerning age, the cumulative drinking amount and the cumulative smoking amount was collected with a questionnaire. The optimum applied amount of dansyl-Cl, the optimum reaction temperature and time, the recoveries and the optimum pH of the eluent and buffer were determined.4-Hyp from human urine was derivatized with dansyl-Cl (dimethylamino-naphthalene-1-sulfonyl chloride) after removing the a-amino acid by a treatment with phthalic dicarboxaldehyde (OPA) and cleaned with Bond Elut C18 column. The 4-Hyp derivatives were separated on a reversed phase column by gradient elution with a phosphate buffer (5 mmol, pH 8.0) and acetonitrile, and detected by fluorescence measurements at 340 nm (excitation) and 538 nm (emission). Results : The detection limit for the urinary free 4-Hyp was $0.364{\mu}mol/l$. The recovery rate of 4-Hyp was 99.7%, and the effective pH of the phosphate buffer and borate buffer were 3.0 and 8.0, respectively. From statistical analysis, age, drinking and smoking did not affect the urinary free 4-Hyp in both the controls and workers. The range of urinary 4-Hyp in the controls, pre-shift, and post-shift workers were 0.33-16.44, N.D-49.06, and $0.32-56.27{\mu}mol/l$. From the pared-sample t-test, the urinary 4-Hyp levels in post-shift workers ($11.82{\pm}6.73\;nmmol/mg\;Cre$) were 2-fold higher than in pre-shift workers ($5.36{\pm}5.53\;nmol;/mg\;Cre$) and controls ($4.91{\pm}4.89\;nmol;/mg\;Cre$). Conclusions : This method was developed with high sensitivity, accuracy, and precision. The present method was effectively applied to analyze the urinary free 4-Hyp in both controls and workers.

Design Optimization of Duplex Burnable Poison Rods and Feasibility Evaluation for Core Design (이중구조 가연성독봉 설계안의 최적화 및 노심 핵설계 타당성 평가)

  • Yoon Seok-Kyun;Lee Dae-Jin;Kim Myung-Hyun
    • Journal of Energy Engineering
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    • v.13 no.4
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    • pp.242-258
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    • 2004
  • The duplex burnable poison absorbers concept was suggested by Korea Atomic Energy Research Institute. This BP rod is composed of inner region of natural U-Gd$_2$O$_3$ and outer shell of enriched UO$_2$-Er$_2$O$_3$. It is expected that this burnable absorber has same reactivity control capability with gadolinia burnable absorber used in extened fuel cycle. In order to evaluate the nuclear feasibility of duplex BPs, the nuclear design characteristics were compared with that of four types of burnable absorbers; gadolinia, erbia, IFBA, dysprosia duplex BP on 24 months fuel cycle for Korean Standard Nuclear Power plants. According to the evaluation results of nuclear characteristics, the duplex BPs were better than other BPs on k-infinitives, reactivity holddown worth (RHW), pin power peaking and moderator temperature coefficient (MTC). The possibility of nuclear core design was also confirmed based on the optimized fuel assemblies which were searched for a sensitivity analysis. Characteristics of core design with duplex BPs was compared with that of reference core with gadolinia BPs for cycle length, power peaking and MTC. The duplex BP core had a little longer cycle length by 4 to 7 days because of increased amount of fissile in enriched uranium at the outer shell of duplex BP In case of power peaking F$\_$Q/ of duplex BP core was reduced from 1.5773 to 1.5335. MTC was also less -0.48 pcm/C than that of reference core. Finally, evaluation of fuel cycle economy was performed for the manufacturing feasibility test and fuel cost evaluation with duplex BPs. Fuel cycle economy of duplex BP core almost was equivalent with that of gadolinia BP core.

Springback Minimization using Bottoming in Al Can Deep Drawing Process (알루미늄 캔 딥드로잉에서 Bottoming을 이용한 스프링백 최소화)

  • Park, Sang-Min;Lee, Sa-Rang;Hong, Seokmoo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.9
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    • pp.302-307
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    • 2016
  • The technology of multistage deep drawing has been widely applied in the metal forming industry, in order to reduce both the manufacturing cost and time. A battery can used for mobile phone production is a well-known example of multistage deep drawing. It is very difficult to manufacture a battery can, however, because of its large thickness to height aspect ratio. Furthermore, the production of the final parts may result in assembly failure due to springback after multistage deep drawing. In industry, empirical methods such as over bending, corner setting and ironing have been used to reduce springback. In this study, a bottoming approach using the finite element method is proposed as a practical and scientific method of reducing springback. Bottoming induces compression stress in the deformed blank at the final stroke of the punch and, thus, has the effect of reducing springback. Different cases of the bottoming process are studied using the finite element program, DYNAFORM, to determine the optimal die design. The results of the springback simulation after bottoming were found to be in good agreement with the experimental results. In conclusion, the proposed bottoming method is expected to be widely used as a practical method of reducing springback in industry.

The research of Correspondence Analysis centered on the Failure Period to improve the reliability of Weapon Systems (무기체계의 신뢰성 향상을 위한 고장발생기간 중심의 대응분석 연구)

  • Song, Bong-Geun;Kim, Geun-Hyung;Kim, Young-Kuk;Park, Seung Hwan;Baek, Jun-Geol
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.10
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    • pp.289-299
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    • 2016
  • Weapon systems require reliability in the development phase for efficient combat readiness. Improved reliability in various manufacturing processes have been achieved using data analysis. However, data analysis in the development phase is difficult due to problems such as the lack of data, high cost, and the importance of security. Therefore, Post Logistics Support (PLS) data collected following integration is analyzed for long-term quality improvement of weapon systems. In this study, we propose a methodology for examining the correlation between the failure rate and PLS data as follows: First, key variables affecting reliability were identified the correlation between variables on the failure rate examined. Second, corresponding analysis was conducted for determining the correlation between patterns of categorical data. Third, extract categories with the higher contribution and quality of representation, and find the highest variable correlated with failure period through visualization. Then, after selecting patterns which have shorter failure period, the cause of decreased reliability was confirmed through frequency analysis. This study will contribute to improving reliability when developing new weapon systems and will help to strengthen the combat readiness of military.

The Development of the Lens of the Optical System for High Concentration Solar PV System (고집광 태양광 발전을 위한 광학시스템 렌즈 개발)

  • Ryu, Kwang-Sun;Cha, Won-Ho;Shin, Goo-Hwan;Cho, Hee-Keun;Kim, Young-Sik;Kang, Seong-Won;Kang, Gi-Hwan
    • Journal of the Korean Solar Energy Society
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    • v.31 no.2
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    • pp.82-88
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    • 2011
  • The artificial increase in the solar intensity incident on solar cells using lenses or mirrors can allow solar cells to generate equivalent power with a lower cost. There are two types of concentration optics for solar energy conversion. One is to use mirrors, and the other is to use Fresnel lenses. The gains that can be achieved with a Fresnel lens or a parabolic mirror are compared. The result showed the gains are comparable and the two configurations were developed competitively. In application areas of Fresnel lenses as solar concentrators, several variations of design were devised and tested. Some PV systems still use commercially available flat Fresnel lenses as concentrators. A convex linear Fresnel lens to improve the concentration ratio and the efficiency is devised and flat linear Fresnel lens in thermal energy collection is utilized. In this study, we designed and optimized flat Fresnel lens and the 'light pipe' to develop 500X concentrated solar PV system. In the process, we compare the transmission efficiencies according to groove types. We performed rigorous ray tracing simulation of the flat Fresnel lenses. The computer aided simulation showed the 'grooves in case' has the better efficiency than that of 'grooves out case'. Based on the ray-trace results we designed and manufactured sample Fresnel lenses. The optical performance were measured and compared with ray-trace results. Finally, the optical efficiency was measured to be above 75%. All the design and manufacturing were performed based on that InGaP/InGaAs/Ge triple junction solar cell is used to convert the photon energy to electrical power. Field test will be made and analyzed in the near future.

Automotive Pre-primed Coatings with Automotive Structural Adhesive for Non-weldable Binding Process (자동차 구조용 접착제를 이용한 자동차용 Pre-primed 도료의 비용접식 접합공정 적용)

  • Moon, Je-Ik;Lee, Yong-Hee;Kim, Hyun-Joong;Noh, Seung Man;Nam, Joon Hyun;Kim, Min-Su;Kim, Jun-Ki;Kim, Jong-Hoon
    • Journal of Adhesion and Interface
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    • v.12 no.3
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    • pp.99-104
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    • 2011
  • Currently, automotive pre-primed coatings has been developed to overcome environmental regulations and to reduce manufacturing cost in automotive industry. By these reasons, an automotive pre-primed system has been investigated to remove the wash and pre-treatment process using a roll coating application. It is required to develop non-weldable pre-primed system for automotive structural adhesives, because pre-primed sheet coated with organic compounds is hard to be assembled by welding process. Primer 1 (polyester type) and primer 2 (urethane type) were designed to satisfy flexibility and formability for non-weldable pre-primed system. According to the results of physical property test of the primers, adhesion test such as single-lap shear test and T-peel test, primer 1 (polyester type) had better physical properties such as pencil hardness, solvent resistance, flexibility and adhesion with automotive adhesive than that of primer 2 (polyurethane type). In addition, the possibility of the non-weldable pre-primed system was applicable to automotive assembly process in place of welding process.

Thermal Insulation and Flame Retardant Properties of Cement Based Super Light-weight Inorganic Thermal Insulation using 100㎛ Grade Glass Bubble (100㎛급 글라스 버블 혼입 시멘트계 초경량 무기 단열재의 단열 및 난연특성)

  • Son, Bae-Geun;Song, Hun
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.9 no.4
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    • pp.642-649
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    • 2021
  • Energy saving standard for buildings are strengthened, the application of exterior insulation finishing system and thickness of insulation materials are increasing. Most buildings with exterior insulation finishing system is applied organic insulating material. Organic insulating material have workability, economic feasibility, reduction in construction cost, and excellent thermal insulation performance. However, Organic insulating material is very vulnerable to heat, so when a fire occurs, rapid fire spread and toxic gas are generated, causing many casualties. Inorganic insulating material can be non-combustible performance, but it is heavy and has low thermal insulation performance. Mineral wool has higher thermal insulation performance than other types of inorganic insulating material, but mineral wool is disadvantageous to workability and vulnerable to moisture. Glass bubble are highly resistant to water and chemically stable substances. In addition, the density of the glass bubble is very low and the particles are spherical, fluidity is improved by the ball bearing effect. Glass bubbles can be used with cement-based ino rganic insulating material to impro ve the weight and thermal insulatio n perfo rmance o f cement-based inorganic insulation. This study produced a inorganic insulating materials were manufactured using cement-based materials and glass bubble. In order to evaluate the insulation performance and flame retardant performance of cement-based super light-weight inorganic insulating materials using with glass bubble, insulation performance or flame retardant and non-combustible performance were evaluated after manufacturing insulating materials using micro cement and two types of glass bubbles. From the test result, Increasing the mixing ratio of glass bubbles improved the insulation performance of cement-based super light-weight inorganic insulating materials, and when the mixing ratio of glass bubbles was 10%, it sho wed sufficient flame retardant and no n-co mbustible perfo rmance.

The Effects of SCM Competency and Process Improvement on Operational Performance in Small and Venture Companies (중소벤처기업의 SCM역량과 프로세스 개선이 운영성과에 미치는 영향)

  • Lee, Seolbin;Park, Jugyeon
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.13 no.6
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    • pp.143-154
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    • 2018
  • This study is intended to look into the effects of SCM(supply chain management) competency and process improvement on operational performance in small and venture companies. To achieve this, a survey was empirically carried out to 179 small and venture manufacturing companies. The findings showed that the SCM competency had a significant effect on the process improvement and operational performance in small and venture companies, adopting all hypotheses. And the process improvement had a significant mediating effect on the relationship between SCM competency and operational performance in small and venture companies, adopting hypothesis 4. As for the findings, strategic alliance, technology development, competency concentration as SCM competencies and starting preparation, detailed planning, implementation management as process improvements were factors that have positive effects on quality performance, cost reduction and profit increase as operational performances in small and venture companies. In other words, the better process and performance by the maximized SCM competencies require selective input strategies for strategic alliance, technology development and competency concentration in small and venture companies. And for its early application and settlement, the starting preparation and detailed planning of business process within small and venture companies need to be jointly put in action under clear company-wide goal management. Consequently, the expected performance can be maximized when strict management and implementation lead to these attributes.

Modeling and Simulation for Predicting the Impact of Hydraulic Breaker (유압 브레이커의 충격량 예측을 위한 모델링과 해석)

  • Kim, Sung-Hyun;Chung, Jaeho;Baek, Dong-Cheon;Park, Jong-Won
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.2
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    • pp.741-749
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    • 2019
  • A hydraulic breaker attached to an excavator is a kind of constructuion equipment which is used for the disassembling of buildings, crashing road pavement, breaking rocks at quarry and etc. Therefore, the performance of the hydraulic breaker is mainly evaluated by the impact quantity and impact efficiency, which is an important factor for both the manufacturer and the user. In this paper, modeling and simulation for the prediction of the impact of the hydraulic breaker was conducted according to hydraulic pressure area and operating conditions of the hydraulic valve and piston using the commercial tools SimulationX for the 20ton hydraulic breaker which is mainly used in construction site. In order to verify the reliability of modeling and simulation, the results of previous experimental studies were compared and verified. The results of this study are expected to be useful for predicting the impact of the hydraulic breaker at the design stage before manufacturing and for studying parameters for improving the impact quantity. In addition, the manufacturer predicts that the development time and cost will be reduced through trial and error prevention by predicting the impact of the hydraulic breaker through the results of this paper.

Economic analysis of integrated data analysis at variety marine protocols (다양한 해양 프로토콜의 분류 및 통합 데이터를 분석한 독립서버에 대한 경제적 분석)

  • Jang, Hyun-Young;Jang, Jong-Wook
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2016.10a
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    • pp.243-246
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    • 2016
  • Data transfer technique is one of the key elements of the IT infrastructure, in accordance with each environment and function, using some kind of protocol. Using the three protocols such as WCDMA, AIS, and TRS at sea IT industry. so It uses three protocols saw has a problem that the operation of each protocol-specific server. so server maintenance costs increase and sea Device Manager has been placed in a situation which operates to develop each of the protocol-specific analysis program. The reason of the protocol is divided into three, marine equipment (lighthouses, buoys, light buoy) manufacturing companies and the date of manufacture, such as, communication environment (distance, communication speed) because such is wrong all, trying to unify this and enormous cost and time is a situation that can not be the exchange occurred when. So in the Ministry of Maritime Affairs and Fisheries, the number of frequency of use is low, communication performance is seeking to refrain from Kure each local organizations and companies the use of low TRS protocol. his paper is intended to conduct research on the basis of the actual data to analyze whether the method is how economical to store a single DB by analyzing other types of protocols. This reduces the shortage of marine IT industry, even a little, shall helps reduce administrative costs.

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