• Title/Summary/Keyword: manganese exposure

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Effect of Myricetin on mRNA Expression of Different Antioxidant Enzymes in B16F10 Murine Melanoma Cells (B16F10 Murine Melanoma Cell에서 Myricetin이 항산화효소의 m-RNA 발현에 미치는 영향)

  • Yu Ji Sun;Kim An Keun
    • YAKHAK HOEJI
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    • v.49 no.1
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    • pp.86-91
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    • 2005
  • Flavonoids are class of polyphenolic compounds widely distributed in the plant kingdom, which display a variety of biological activities, including antiviral, antithrombotic, antiinflammatory, antihistaminic, antioxidant and free-radica 1 scavenging abilities. The antioxidant enzyme (AOE) system plays an important role in the defense against oxidative stress insults. To determine whether flavonoid, myricetin can exert antioxidative effects not only directly by modulating the AOE system but also scavenging free radical, we investigated the influence of the flavonoid myricetin on cell viability, different antioxidant enzyme activities, ROS level and the expression of different antioxidant emzyme in B16F10 murine melanoma cells. Myricetin in a concentration range from 6.25 to $50\;{\mu}M$ decreased superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzyme activities, but catalase (CAT) activity was increased. In the myricetin-treated group, ROS levels were decreased dose-dependently. Antioxidant enzyme expression was measured by RT-PCR. Myricetin treatment of B16F10 cells increased catalase expression. Expression levels of copper zinc superoxide dismutase (CuZn SOD) were not affected by exposure of myricetin. Manganese superoxide dismutase (Mn SOD) and GPx expression levels decreased slightly after myricetin treatment. In conclusion, the antioxidant capacity of myricetin was due to CAT and free-radical scavenging.

Heavy Metal Contamination of Feral Pigeons Columba livia by Habitat in Seoul (서울지역 집비둘기 Columba livia의 서식지별 중금속 오염)

  • Kim, Jeong-Su;Han, Sang-Hui;Lee, Du-Pyo;Gu, Tae-Hoe
    • The Korean Journal of Ecology
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    • v.24 no.5
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    • pp.303-307
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    • 2001
  • Some Feral Pigeons Columba livia were collected in commercial, industrial, park and resident areas of Seoul and heavy metal concentrations in their liver, kidney, bone and muscle tissues were measured to compare the levels of exposure by habitats. Statistical analyses showed some differences in the levels of heavy metals among these habitats. The levels of iron in liver and zinc in kidney were lower in resident area than in the other ones. Manganese and copper levels in all tissues were the highest in industrial area and park area, respectively. Lead bone level in resident area was the lowest, while that in commercial area was the highest. Cadmium level in kidney was higher in park area than in the other areas. Of these results, the levels of iron, zinc and lead in some tissues were related to traffic density in each habitats.

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A Case of Metal Fume Fever Associated with Copper Fume in a Welder (용접공에서 발생한 구리흄에 의한 금속열 1례)

  • Lim, Hyun-Sul;Cheong, Hae-Kwan
    • Journal of Preventive Medicine and Public Health
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    • v.31 no.3 s.62
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    • pp.414-423
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    • 1998
  • Metal fume fever has been known as an occupational disease is induced by intense inhalation of fresh metal fume with a particle size smaller than $0.5{\mu}m\;to\;1{\mu}m$. The fumes originate from heating metals beyond their boiling point, as happens, for example, in welding operations. Oxidation usually accompanies this process. In most cases, this syndrome is due to exposure to zinc oxide fumes; however, other metals like copper, magnesium, cadmium, manganese, and antimony are also reported to produce such reactions. Authors report a case of metal fume fever suspected to be associated with copper fume inhalation. The patient was a 42-year-old male and was a smoker. He conducted inert gas tungsten arc welding on copper-coated materials without safety precautions such as a protective mask and adequate ventilation. Immediately after work, he felt metallic taste in his mouth. A few hours after welding, he developed headache, chilling sensation, and chest discomfort. He also complained of myalgia, arthralgia, feverish sensation, thirst, and general weakness. Symptoms worsened after repeated copper welding on the next day and subsided gradually following two weeks. Laboratory examination showed a transient increase of neutrophil count, eosinophilia, elevated erythrocyte sedimentation rate, and positive C-reactive proteinemia. Blood and urine copper level was also increased compared to his wife. Before this episode, he experienced above complaints several times after welding with copper materials but welding of other metals did not produce any symptoms. It was suggested that copper fume would have induced metal fume fever in this case. Further investigations are needed to clarify their pathogenic mechanisms.

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A Study on Concentrations of Heavy Metal in Blood and Urine of Local Area in Korea (국내 일부 지역주민의 혈액과 요중 중금속 농도에 관한 연구)

  • Im, Ji-Young;Chung, Eun-Kyung;Park, Hee-Jin;Yu, Seungdo;Jang, Bong-Ki;Son, Bu-Soon
    • Journal of Environmental Science International
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    • v.22 no.1
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    • pp.59-72
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    • 2013
  • The purpose of this study is to assess the effects of heavy metal concentrations in the blood and urine of the general population. This research had been conducted from April to December 2008, studying 545 residents of Daejeon and Chungcheong Province. Through the concentrations of heavy metals(Pb, Cd, Hg, As, Mn) in the biota samples and questionnaires, the residents heavy metal exposure level and the influential factors according to personal characteristics or lifestyle were evaluated. As to the heavy metal concentration in the blood and urine of the comparing region, were As and Mn statistically significant(p<0.01, p<0.05). Blood lead and urinary mercury concentrations were higher in males than females. The heavy metal concentration for each age group increased blood mercury. The concentration of all heavy metals were higher in the drinkers than in the non-drinkers. Blood lead and mercury concentrations were higher in the smokers than in the non-smokers, but the urinary cadmium, arsenic and blood manganese was higher in the non-smokers than in the smokers. As to the blood lead and urinary cadmium concentration according to the food preference fish showed high concentration. To clarify the factors affecting the heavy metal concentration in biota among subjects multiple regression analysis was conducted. As a results, it turned out that as to lead content in blood, sex, age and smoking have influence on the subjects with explanatory adequacy of 14.0 %. These results demonstrated that the factors affected the concentrations of heavy metals in blood and urine. The results of this study could be used as the foundational data for setting the health risk assessment.

Exposure Evaluation to Total Welding Fume and Manganese at Technical High Schools in Choong-Nam Area (충청지역 일부 공업고등학교 실습생의 용접흄 및 망간에 대한 노출 평가)

  • 이종화;박종안;장봉기
    • Proceedings of the Korean Environmental Health Society Conference
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    • 2002.04a
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    • pp.37-39
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    • 2002
  • Geometric mean of airborne welding fume concentration at technical high schools was 4.80mg/㎥(N.D∼35.39mg/㎥) and the percentage of samples exceeded TLV of the Korean ministry of labor was 43.6%, Geometric mean of airborne Mn concentration was 0.06mg/㎥(N.D∼0.42mg/㎥) and the percentage of samples exceeded TLV of ACGIH was 15.4%. In case of airborne Mn concentration, there is a significant difference among schools (P<0.05). Mn concentrations in blood of the exposed and control groups were 1.84$\mu\textrm{g}$/㎗ and 1.91$\mu\textrm{g}$/㎗, respectively. Mn concentrations in urine of the exposed and control groups were 1.36$\mu\textrm{g}$/$\ell$ and 0.57$\mu\textrm{g}$/$\ell$, respectively. In case of Mn concentrations in urine, there is a significant difference between both groups(P<0.001) and among schools(P<0.05). Mn concentrations in blood and urine of exposed group were not over BEIs of the Korean ministry of labor. Mn levels in blood and urine were not significantly affected by smoking, drinking and residence. There was no correlation between Mn concentration in air and blood, but there was a statistically significant correlation between Mn concentration in air and urine(r=0.323). There was no a Statistically significant correlation between Mn concentration in blood and urine.

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The Action and Pacemaker Potential in the Frog Truncus Arteriosus (개구리 대동맥의 활동전압 및 Pacemaker 전압에 관한 연구)

  • Earm, Yung-E;Sung, Ho-Kyung
    • The Korean Journal of Physiology
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    • v.16 no.2
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    • pp.119-128
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    • 1982
  • The frog truncus arterious were studied with conventional glass microelectrode technique in order to elucidate the underlying mechanism of spontaneous pacemaker activity. The analyses were focussed on the ionic nature of pacemaker current by changing the concentrations of extracellular $K^+$ and, $Na^+$, or by using blockers of K- and Ca-current and chronotropic transmitters. 1) The action potential of the spontaneously active truncus arteriosus has some characteristic feature of maximal distolic potential ranged from -65 to -75 mV, resting potential from -45 to -50 mV and overshoot voltage about +30 mV, respectively. Duration of the action potential taken from rapid upstroke to maximal diastolic potential was about 600 msec. Usual discharge rate was $25{\sim}30/min$ at room temperature $(18{\sim}20^{\circ}C)$. 2) The sensitivity of the resting membrane potential to change extracellular potassium concentrations $(0{\sim}12\;mM)$ was relatively low. Transient hyperpolarization was appeared in the 12 mM K Ringer after 10 min exposure to 0 mM K and it could be related to Na-pump reactivation by high potassium. 3) Reduction of extracellular sodium concetrations diminished the amplitude and frequency of the action potential. In Ringer solution containing 30% Na (substituted by equimolar Tris), spontaneous activity stopped but reappeared as very slow and small action potential. There was no spotaneous activity in zero Na Ringer solution. 4) Caesium(10 mM), K-current blocker decreased the frequency of the action potential and also pacemaker depolarization. Manganese (2 mM) known to be Ca-current antagonist, blocked spontaneous activity completely. 5) Adrenaline and acetylcholine had no chronotropic effect. But adrenaline increased the duration of plateau phase and the magnitude of the action potential in the follower cell. It is concluded that K-, Na-and Ca-current components are involved in the genesis of spontaneous activity of the frog truncus arteriosus like cardiac pacemaker tissues. But the insensitivity of truncus arteriosus to adrenaline and acetylcholine indicates that there are some different control mechanisms of spontaneous rhythm in two tissues.

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Characteristic of Inorganic Contaminants and Conservation Treatment Materials for the Three-Storied Stone Pagoda of Bulguksa Temple in Gyeongju (경주 불국사 삼층석탑(석가탑)의 무기오염물과 보수물질 특성)

  • Lee, Gemma;Kim, Sa Duk;Park, Sungchul;Kim, Derk Moon
    • Journal of Conservation Science
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    • v.34 no.5
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    • pp.421-431
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    • 2018
  • Analyses of the three-storied stone pagoda at the Bulguksa temple in Gyeongju were carried out in order to identify the cause for material characteristics of inorganic contaminants and conservation treatment materials. Results indicated that foreign soil or weathering soil caused yellow discoloration of the pagoda, reddish-brown contaminants were formed by goethite (FeOOH), an iron oxide mineral, and black pollutants were formed by manganese (Mn) oxide, leading to discoloration of the rock. Among the restoration materials used in the past, cement mortar could cause whitening by reacting with the external environment. The results were used as basic standards to evaluate the material characteristics of the surface contaminants and identify a set of effective conservation treatments. Nevertheless, continuous monitoring is required, as there is a high possibility of regeneration of pollutants in the future because of the continuous exposure of the pagoda to the external environment.

A Study on Factors Affecting Airborne Fume Composition and Concentration in Welding Process (용접공정에서 발생된 공기중 흄의 조성과 농도에 영향을 미치는 요인에 관한 연구)

  • Shin, Yong Chul;Yi, Gwang Yong;Park, Seung Hyun;Lee, Na Roo;Jeong, Jee Yeon;Park, Jung Keun;Oh, Se Min;Moon, Young Hahn
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.7 no.2
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    • pp.181-195
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    • 1997
  • The purpose of this study was to investigate factors affecting the composition and concentrations of fumes generated from various types of welding processes. The results are as follows. 1. Iron(Fe), zinc(Zn) and manganese(Mn) were predominant in Welding fumes. The Fe content in total fumes was 25.5% in coated electrode and 28.2% in $CO_2$ are welding, and the Zn content was 4.5% and 9.1%, respectively, and the Mn was 3.6% and 7.8%, respectively. 2. It was found that the important factors determining composition and concentration of fumes were type of industries, type of welding processes, type and composition of electrodes, composition of base metals, confinement of workplaces or condition of ventilation, work intensity, coated metals such as lead and Zn in paint. 3. The Mn content in airborne fumes was highly correlated with that of electrode(r=0.77, p<0.01) and was about 4 times higher than that in electrodes or base metals. The results lindicate that Mn is well evaporated into air during welding. The higher vapor pressure of Mn may explain this phenomenon. 4. the airborne total fume concentrations were significantly different among types of industries(p<0.001). The airborne total fume concentration was higher in order of sleel-structure manufacturing($GM=15.1mg/m^3$), shipbuilding($GM=13.2mg/m^3$), automobile-component manufacturing ($GM=7.8mg/m^3$) and automobile assembling industry($GM=3.0mg/m^3$) 5. The airbone total fume concentration was 6 times higher in $CO_2$ welding than in coated electrode welding, and approximately 3 times higher in confined area than in open area, in steel-structure manufacturing industry. 6. The concentration of welding fume outside welding helmet was about 2 times higher than that inside it. It is recommened that air sampling be done inside helmet to evaulate worker's exposure accurately, for it has an outstanding effect on reducing worker exposure to fumes and other contaminants.

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Prioritizing Management Ranking for Hazardous Chemicals Reflecting Aggregate Exposure (통합노출을 고려한 유해물질 관리의 우선순위 선정)

  • Jeong, Ji-Yoon;Jung, Yoo-Kyung;Hwang, Myung-Sil;Jung, Ki-Kyung;Yoon, Hae-Jung
    • Journal of Food Hygiene and Safety
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    • v.27 no.4
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    • pp.349-355
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    • 2012
  • In this study, we configured a system which ranks hazardous chemicals to determine their management priorities based on experts' opinions and the existing CRS (chemical ranking and scoring). Aggregate exposure of food, health functional food, oriental/herbal medicine and cosmetics have been taken into account to determine management priority. In this study, 25 hazardous chemicals were selected, such as cadmium, lead, mercury, and arsenic, etc. These 25 materials were ranked according to their 1) risk (exposure or hazard) indexes, 2) exposure source-based weight, and 3) public interests, which were also formed based on the existing priority ranking system. Cadmium was scored the highest (178.5) and bisphenol A the lowest (56.8). Ten materials -- cadmium, lead, mercury, arsenic, tar, acrylamide, benzopyrene, aluminium, benzene, and PAHs -- scored higher than 100. Eight materials -- aflatoxin, manganese, phthalate, chromium, nitrate/nitrite, ethylcarbamate, formaldehyde, and copper -- recorded scores in the range from 70 to 100. Also evaluated as potential risks were 7 materials; sulfur dioxide, ochratoxin, dioxins, PCBs, fumonisin, methyl mercury, and bisphenol A, and these materials were scored above 50. Then we compared risk index and correlation coefficient of total scores to confirm the validity of the total scores; we analyzed correlation coefficient of parameter and indicator. We discovered that the total score and weight, which has incorporated public interests, were high and statistically significant. In conclusion, the result of this study contributes to strengthening risk assessment and risk management of hazardous chemicals.

Expression Pattern of Antioxidant Enzymes Genes in the Ventral Prostates of Rats Exposed to Procymidone and/or Testosterone after Castration

  • Lee, Jong-Geol;Yon, Jung-Min;Jung, Ki-Youn;Lin, Chunmei;Jung, A-Young;Lee, Beom-Jun;Yun, Young-Won;Nam, Sang-Yoon
    • Journal of Embryo Transfer
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    • v.26 no.4
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    • pp.265-270
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    • 2011
  • Procymidone is a fungicide with anti-androgenic properties widely used to protect fruits from fungal infection, which induces an excessive reactive oxygen species production in male reproductive organs. In this study, to clarify whether procymidone affect the cellular antioxidant system of prostate at onset of puberty, gene expression patterns of the representative antioxidant enzymes such as cytoplasmic glutathione peroxidase (GPx1), phospholipid hydroperoxide GPx (PHGPx), selenoprotein P (SePP), cytoplasmic copper/zinc superoxide dismutase (SOD1), and manganese SOD (SOD2) were investigated in the rat ventral prostates exposed to procymidone using real-time RT-PCR analyses. Seven-week-old Sprague-Dawley rats castrated at 6 weeks old were treated with procymidone (25, 50, or 100 mg/kg per day) orally for 7 consecutive days after testosterone propionate (0.4 mg/kg per day) administration by subcutaneous injection. As compared to normal control animals, GPx1 mRNA expression in prostates significantly increased by the administration with TP and/or procymidone. However, PHGPx and SOD1 mRNA levels significanatly decreased by over 25 mg/kg of procymidone treatment and SePP and SOD2 mRNA levels was significanatly reduced by over 50 mg/kg of procymidone treatment. These findings indicate that procymidone may affect the antioxidant system of prostatic cells in up-regulation mode of GPx1, but in down-regulation modes of PHGPx, SePP, SOD1, and SOD2, suggesting that procymidone may affect differently the cellular antioxidant system of prostate according to the exposure doses.