• Journal of Nutrition and Health
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• 제34권5호
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• pp.525-531
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• 2001

The present study investigated the protective effects of green tea against acute ethanol-induced lipid peroxidation and the change of antioxidative enzyme activities in various regions of rat brain : cortex, cerebellum, striatum and hippocampus. The following parameters were examined : malondialdehyde(MDA) levels and activities of superoxide dismutase(SOD), catalase and glutathione peroxidase(GSH-Px). Male Sprague-Dawley rats were given the experimental containing 1% green tea powder or control diet for 4 weeks, and at the end of feeding diet group received acute ethanol(5g/kg body weight) or equicaloric sucrose solution intragastrically. Green tea powder significantly decreased MDA levels in the striatum compared to control-non alcohol treated group to 1% green tea-non alcohol treated group without altering the antioxidative enzyme activities. Green tea resulted in a significant increase in GSH-Px activities in the hippocampus compared to either control-non alcohol treated group(0.043units/mg protein) or 1% green tea-non alcohol treated group(0.071units/mg protein). In conclusion, these results suggest that moderate consumption of green tea leaves can exert protective effects against ethanol-induced oxidative stress in brain regions, by reducing MDA concentrations in the striatum and enhancing GSH-Px activities in the hippocampus. (Korean J Nutrition 34(5) : 525∼531, 2001)

• Journal of Ginseng Research
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• 제37권2호
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• pp.194-200
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• 2013

Korean red ginseng (KRG) is prepared by the process of steaming the roots of Panax ginseng. In this study, the feeding effects of KRG-water extract (KRGE) on ethanol-induced liver damage were elucidated by measuring serum biomarkers in rats. Serum ${\gamma}$-glutamyltranspeptidase (g-GT) activity and the concentration of malondialdehyde (MDA) were significantly increased by short-term and long-term ethanol treatment in rats, whereas the activities of serum glutamate pyruvate transaminase (GPT) and glutamate oxaloacetate transaminase (GOT) did not respond. Pretreatment with KRGE maintained the activity of serum GPT, and the MDA concentration induced by short-term ethanol ingestion remained within the normal range. However, co-feeding of KRGE to rats decreased the concentration of MDA but failed to modulate the serum ${\gamma}$-GT activity induced by long-term ethanol treatment. Our studies suggest that in rats, it appears that KRGE does not sufficiently reverse the physiological response evoked by long-term ethanol ingestion to maintain normal conditions, in view of the serum biomarker ${\gamma}$-GT, regardless of KRGE's favorable antioxidant activity.

• Preventive Nutrition and Food Science
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• 제17권2호
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• pp.158-165
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• 2012

This study was designed to investigate whether the fatty acid composition could make a significant contribution to the oxidation stability of vegetable oils marketed in Korea. Ten kinds, 97 items of vegetable oils that were produced in either an industrialized or a traditional way were collected and analyzed for their fatty acid compositions and lipid oxidation products, in the absence or presence of oxidative stress. Peroxidability index (PI) calculations based on the fatty acid composition ranged from 7.10 to 111.87 with the lowest value found in olive oils and the highest in perilla oils. In the absence of induced oxidative stress, malondialdehyde (MDA), the secondary lipid oxidation product, was generated more in the oils with higher PI (r=0.890), while the tendency was not observed when the oils were subjected to an oxidation-accelerating system. In the presence of the oxidative stress, the perilla oils produced in an industrialized manner generated appreciably higher amounts of MDA than those produced in a traditional way, although both types of oils presented similar PIs. The results implicate that the fatty acid compositions could be a predictor for the oxidation stability of the vegetable oils at the early stage of oil oxidation, but not for those at a later stage of oxidation.

• 대한통합의학회지
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• 제6권4호
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• pp.75-82
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• 2018

Purpose : The present study tested the hypothesis that acupressure at Zusanli(ST36) would attenuate immobilization-induced skeletal muscle atrophy. Methods : The left hind limb was immobilized using casting tape (ST36 group, n=10), and the animals were then treated daily with a pressure needle at ST36. Ten untreated animals with hind limb immobilization and no treatment served as a control group (n=10). After 2 weeks of immobilization, The body weight changes of rats were evaluated and the morphologies of the right and left gastrocnemius muscles in both the ST36 and control groups were assessed by hematoxylin and eosin staining. The levels of malondialdehyde (MDA) of gastrocnemius muscles were observed. Results : The acupressure at the accupoint of Zusanli conferred significant protection against reductions in left gastrocnemius muscle weights and average cross-sectional muscle areas in the ST36 group as compared with those in the control group. Moreover, the acupressure at the ST36 point significantly reduced malondialdehyde (MDA) activity in the gastrocnemius muscles as compared with that in the control group. Conclusion : These results suggest that acupressure at the accupoint of Zusanli provides protection against immobilization-induced muscle atrophy by decreasing MDA activity in gastrocnemius muscles.

• Journal of Preventive Medicine and Public Health
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• 제49권5호
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• pp.260-274
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• 2016

Objectives: Previous epidemiological studies about oxidative stress and depression are limited by hospital-based case-control design, single-time measurements of oxidative stress biomarkers, and the small number of study participants. Therefore, in this study, we analyzed the association between biomarker of oxidative stress and depressive symptom scores using repeatedly measured panel data from a community-dwelling elderly population. Methods: From 2008 to 2010, a total of 478 elderly participants residing in Seoul, Korea, were evaluated three times. Participants underwent the Korean version of the Short Form Generic Depression Scale (SGDS-K) test for screening depression, and urinary malondialdehyde (MDA) levels were measured as an oxidative stress biomarker. We used a generalized estimating equation with a compound symmetry covariance structure to estimate the effects of oxidative stress on depressive symptom scores. Results: A two-fold increase in urinary MDA concentration was significantly associated with a 33.88% (95% confidence interval [CI], 21.59% to 47.42%) increase in total SGDS-K scores. In subgroup analyses by gender, a two-fold increase in urinary MDA concentration was significantly associated with increased SGDS-K scores in both men and women (men: 30.88%; 95% CI, 10.24% to 55.37%; women: 34.77%; 95% CI, 20.09% to 51.25%). In bivariate analysis after an SGDS-K score ${\geq}8$ was defined as depression, the third and the fourth urinary MDA quartiles showed a significantly increased odds ratio(OR) of depression compared to the lowest urinary MDA quartile (third quartile OR, 6.51; 95% CI, 1.77 to 24.00; fourth quartile OR, 7.11; 95% CI, 1.99 to 25.42). Conclusions: Our study suggests a significant association between oxidative stress and depressive symptoms in the elderly population.

• Archives of Pharmacal Research
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• 제27권3호
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• pp.340-345
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• 2004

Our work in this study was made in the microsomal fraction to evaluate the lipid peroxidation by measuring superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) and to elucidate the preventive role of CS in the $CCl_4$-induced oxidative stress. The excessive lipid peroxidation by free radicals derived from $CCl_4$ leads to the condition of oxidative stress which results in the accumulation of MDA. MDA is one of the end-products in the lipid peroxidation process and oxidative stress. MDA, lipid peroxide, produced in this oxidative stress causes various diseases related to aging and hepatotoxicity, etc. Normal cells have a number of enzymatic and nonenzymatic endogenous defense systems to protect themselves from reactive species. The enzymes in the defense systems, for example, are SOD, CAT, and GPx. They quickly eliminate reactive oxygen species (ROS) such as superoxide anion free radicalㆍO$^{[-10]}$ $_2$, hydrogen peroxide $H_2O$$_2$ and hydroxyl free radicalㆍOH. CS inhibited the accumulation of MDA and the deactivation of SOD, CAT and GPx in the dose-dependent and preventive manner. Our study suggests that CS might be a potential scavenger of free radicals in the oxidative stress originated from the lipid peroxidation of the liver cells of $CCl_4$-treated rats.

• 한국환경과학회지
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• 제5권5호
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• pp.635-642
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• 1996

UV-B증가가 지질과산화에 미치는 영향과 UV-B조사에 대한 carotenoid 및 polyamine의 지질과산화 방어반응을 조사하기 위해 자연광 이용의 인공기상실내에서 12일간 벼 (Oryza sativa L., cv. Koshihikari) 유식물에 2단계의 UV-B조사 실험을 수행했다. UV-B처리에 의한 건물량의 감소는 조사시간의 경과와 함께 증가하였다. 지질과산화산물인 malondialdehyde (MDA)의 함량은 6일간의 UV-B처리에 의해 약 30 %의 증가를 보였다. Carotenoid의 함량은 UV-B처리에 의해 약 10% 감소하는 경향을 보였으나 통계학적 유의성은 없었다. 한편, 벼에는 크게 3종류의 polyamines (putrescine, spermidine, spermine)이 존재하였으며, 이들 모두 UV-B처리에 의해 상당히 증가되었다. 이상의 결과로 볼 때, UV-B조사에 의해 생성된 활성산소가 생체막지질의 산화에 관여하는 것으로 사료되며, polyamine의 증가는 UV-B조사에 의한 세포막 파괴를 막기 위한 생화학적 방어반응으로 사료된다.

• 생명과학회지
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• 제21권6호
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• pp.838-844
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• 2011

본 연구는 고강도 1회성 운동 시 혈장 MDA와 SOD의 농도변화와 임파구 DNA 손상에 대한 성별의 차이를 평가하는데 목적이 있었다. 본 연구의 목적을 달성하기 위하여 남자 대학생과 여자 대학생을 대상으로 85%$VO_{2max}$ all-out 운동수행에 따른 혈장 MDA와 SOD 그리고 임파구 DNA 손상에 대한 분석을 실시하였으며, 연구 결과에 대한 결론은 다음과 같다. 85%$VO_{2max}$ all-out 운동에 따른 혈장 MDA와 SOD는 운동 종료 시 유의하게 증가하였으며, 통계적으로 유의한 차이는 나타나지 않았으나 남성이 여성에 비해 MDA는 높고 SOD는 낮은 경향을 보였다. 반면 85%$VO_{2max}$ all-out 운동에 따른 임파구 DNA 손상을 알아보기 위해 실시한 comet assay 결과 세 가지 parameter (%DNA in the tail, tail length, tail moment) 모두 운동 종료 시 유의하게 증가하였으며 남성의 %DNA in the tail과 tail length가 여성에 비해 통계적으로 유의하게 높게 나타났다. 따라서 본 연구 결과를 종합해보면 1회성 고강도 운동은 산화적 스트레스를 유발할 수 있으며 남성이 여성에 비해 산화적 손상이 더 크다고 보여진다. 그러나, DNA 손상에는 산화적 스트레스 외에도 체력, 호르몬 수치, 생활습관, 운동 강도 및 지속시간 등 여러 가지 요인들이 영향을 줄 수 있다고 보고되고 있어, 성별에 따른 DNA 손상에 대한 명확한 기전을 제시하기 위해서는 DNA 손상에 영향을 줄 수 있는 여러 요인들과의 관계를 고려한 지속적인 연구들이 필요하다고 생각된다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2001년도 International Symposium on Signal transduction in Toxicology
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• pp.136-137
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• 2001

MDA(malondialdehyde) is the parameter of lipid peroxidation. The change of MDA value was investigated in normal and in fibrotic rats by bile duct ligation and scission operation. In order to know how does lipid peroxidation play under the fibrotic condition we studied whether MDA as lipid peroxidation marker has correlation with one of the liver fibrotic parameters.(omitted)

• 대한한방부인과학회지
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• 제27권3호
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• pp.116-123
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• 2014

Objectives: This study aims to research correlation of the malondialdehyde level in urinalysis with cold hypersensitivity. Methods: We studied 128 patients visiting ${\bigcirc}{\bigcirc}$ Korean Hospital from March, 2013 to May, 2013. The subjects were categorized into two groups: cold hypersensitivity group (n=46) and control group (n=79). Patients in cold hypersensitivity group had been stressed because of severe cold hypersensitivity. First, We investigated the difference of the level of malondialdehyde between two groups. In addition, temperature of the two points, ST32 and LR3, was measured by using Digital Infrared Thermal Imaging (DITI) to find out whether the extent of cold hypersensitivity is related to the level of malondialdehyde. Results: There is no different characteristics between two groups. The average of malondialdehyde in cold hypersensitivity group ($1613.15{\pm}1260.71$) is significantly higher than in control group ($1170.01{\pm}1015.66$)(p=0.04). But there is no correlation between level of malondialdehyde and the extent of cold hypersensitivity (r=-0.22, p=0.13). Conclusions: Although the level of malondialdehyde in cold hypersensitivity group is higher than in control group, there is no significant correlation between level of malondialdehyde and the extent of cold hypersensitivity.