Effects of UV-B radiation on carotenoids, polyamines and lipid peroxidation in rice (Oryza sativa L.) leaves

UV-B가 벼잎의 carotenoid, polyamine 및 지질과산화에 미치는 영향

  • 김학윤 (경북대학교 농업과학기술연구소)
  • Published : 1996.10.01

Abstract

Rice plants, cv. Koshihikari, were subjected to the biologically effective ultraviolet-B(UV-BBE) radiation {daily dose : 0.0 (control) and 11.5 (enhanced UV-B) KJ m-2} to investigate the effect of enhanced UV-B radiation on lipid peroxidation and to determine whether carotenoids and polyamines are involved in protection mechanism against enhanced UV-B radiation. Enhanced UV-B radiation significantly depressed plant dW weight. Malondialdehyde (MDA) content in rice leaves was increased by about 30% after 6 days of UV-B irradiation. Total carotenoid contents tended to slightly decrease with the UV-B irradiation, even though there was no significance. In rice leaves, 3 major polyamines, putrescine, spermidine and spermine are observed. All of the polyamine contents were increased with UV-B irradiation. The results suggest that enhanced UV- B radiation caused oxidative stress on lipids and that polyamines may serve as a biochemiral protectant against increased UV-B radiation in rice plants.

UV-B증가가 지질과산화에 미치는 영향과 UV-B조사에 대한 carotenoid 및 polyamine의 지질과산화 방어반응을 조사하기 위해 자연광 이용의 인공기상실내에서 12일간 벼 (Oryza sativa L., cv. Koshihikari) 유식물에 2단계의 UV-B조사 실험을 수행했다. UV-B처리에 의한 건물량의 감소는 조사시간의 경과와 함께 증가하였다. 지질과산화산물인 malondialdehyde (MDA)의 함량은 6일간의 UV-B처리에 의해 약 30 %의 증가를 보였다. Carotenoid의 함량은 UV-B처리에 의해 약 10% 감소하는 경향을 보였으나 통계학적 유의성은 없었다. 한편, 벼에는 크게 3종류의 polyamines (putrescine, spermidine, spermine)이 존재하였으며, 이들 모두 UV-B처리에 의해 상당히 증가되었다. 이상의 결과로 볼 때, UV-B조사에 의해 생성된 활성산소가 생체막지질의 산화에 관여하는 것으로 사료되며, polyamine의 증가는 UV-B조사에 의한 세포막 파괴를 막기 위한 생화학적 방어반응으로 사료된다.

Keywords

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