• Title/Summary/Keyword: male sterile

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Effects of Aucuba japonica Extract on Oral Wound Healing (구강점막의 창상치유에 대한 식나무(Aucuba japonica) 추출물의 효과)

  • Shim Kyung-Mi;Kim Se-Eun;Choi Jeong-Yun;Park Joo-Cheol;Jeong Soon-Jeong;Lee Jae-Yeong;Bae Chun-Sik;Park Don-Hee;Kim Do-Man;Jeong Moon-Jin;Kang Seong-Soo
    • Journal of Veterinary Clinics
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    • v.23 no.1
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    • pp.55-60
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    • 2006
  • Aucuba japonica has variable pharmacological effects such as hepatoprotective, choleretic, hemodynamic, antimicrobial, and anti-inflammatory activities. This study was performed to investigate the effects of Aucuba japonica extract on oral wound healing. Aucubin was extracted from Aucuba japonica, and injected on either side of buccal mucosa of male mice. Artificial full thickness wounds were made on the site with 1.5 mm biopsy punch under sterile technique. The specimens had taken on day 1, 3, and 5 with 4 mm biopsy punch. Light microscopic examination and quantitative histologic analysis were performed for reepithelization, inflammatory cell infiltration. Reepithelization of the aucubin (0.1%) group was earlier than the control group. And the number of inflammatory cells of the aucubin group was lesser than the control group. In view of the results so far achieved, the aucubin extracted from Aucuba japonica may be useful for oral wound healing and it can be applied as a topical agent on the oral wound. Further research should be performed on the mechanism of aucubin on oral wound healing and proper formulation for effective topical agents.

Integrated Pest Management Against Bactrocera Fruit Flies (Bactrocera 속 과실파리 종합관리기술)

  • Kim, Yonggyun;Kim, Dong-Soon
    • Korean journal of applied entomology
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    • v.55 no.4
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    • pp.359-376
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    • 2016
  • Increase in world trade commodities along with climate change entails frequent pest insect invasions from subtropical to temperate zones. Tephritidae is one of two families in fruit flies along with Drosophilidae and consists of more than 5,000 species. Some tephritid flies in genera Anastrepha, Rhagoletis, Ceratitis, and Bactrocera are highly invasive and give serious economic damages on fruits and vegetables in temperate zones. This review focuses mainly on Bactrocera fruit flies, which have been well studied in control techniques to defend their invasion and colonization. Though various control techniques against Bactrocera fruit flies have been developed, less efforts have been paid to establish efficient integrated pest management (IPM) programs. This review introduces current control techniques of fruit flies and some successful IPM programs implemented in subtropical islands. In addition, major fruit flies infesting crops in Korea have been also reviewed in their occurrence and control programs.

Study on Stable Fly Eradication by Sterile-male Technique (4) Effects of X-ray Irradiation on the Stable Fly (웅성불임기술을 이용한 쇠파리 구제에 관한 연구 (4) 방사선 조사가 쇠파리에 미치는 영향)

  • Chung K. H.;Ryu J.;Kwon S. H.
    • Korean journal of applied entomology
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    • v.14 no.3 s.24
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    • pp.163-166
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    • 1975
  • This experiment was performed to investigate the X-ray sensitivities at the various stages of life cycle and to determine the sterillizing dose of stable fly, Stomoxys calcitrans(L). A dose of 300 rad caused about $50\%$ mortality in 2-hour-old eggs as measured by egg hatch, and $100\%$ mortality was obtained with a does of 1 Krad. Sub-lethal dose $(LD_{50})$ for the pupal mortality after irradiation of 3-5days old pupae were 11.5 Krad and the mortality was decreased with increasing pupal age at irradition. A significant reduction of egg hatch by $1.5\%$ was observed when treated males with 3 Krad at pupal stage were mated to untreated virgin femleas. On the other hand, $100\%$ sterility in females was resulted by 2 Krad irradiation and oviposition was completely inhibited with 3 Krad. Thus, both sexes of stable fly could be sterilized with a dose of 4 Krad irradiated to 3-5 days old pupae.

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New Mid-late Maturing F1 Hybrid Onion Cultivar, "Yeongpunghwang" (양파 추파형 중·만생 다수성 신품종 "영풍황")

  • Lee, Eul-Tai;Kim, Cheol-Woo;Choi, In-Hu;Jang, Young-Seok;Bang, Jin-Ki;Bae, Sang-Gyeong;Hyun, Dong-Yun;Jung, Jong-Mo;Ha, In-Jong;Kim, Seong-Bae
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.587-590
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    • 2009
  • New onion hybrid cultivar, "Yeongpunghwang" was developed from cross between "MOS8", male sterile line and "Mokpo 11", pollen line by Mokpo Sub-Station, National Institute of Horticultural & Herbal Science, RDA in 2008. The crossing line was performed yield test in 2003. IT was evaluated as "Mokpo 11" in the regional yield trial test(RYT) from 2006 to 2008 during three years. This line is semi-erect plant growth type and has green leaf colour. It has broad ovate bulb shape and yellow skin colour in bulb characteristics. Plant height, leaf number, and bolting rate was 78cm, 7.9, and 3.9%, respectively. Lodging date as harvesting indicator was on 26 May. The yield potential of "Yeongpunghwang" was out-yielding 6% compare to check variety ("Sunpower") by with 8,344 kg/10a in RYT. It showed moderated cold hardness in all regional location which was conducting RYT, including Muan, Goheung, Changyeong and Andong. So this variety was recommend as a leading variety at southern area of korea.

Agronomic Characteristics of A Mutant for Genic Male Sterility-Chalky Endosperm and Its Utilization on $F_1$ Hybrid Breeding System in Rice (벼 유전자적 웅성불임-심백 돌연변이체의 특성과 그를 이용한 일대잡종 육성체계)

  • ;Mun-Hue Heu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.6
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    • pp.684-696
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    • 1995
  • A mutant for chalky endosperm and genetic male sterility (GMS) was newly developed in rice. The two characters were found to be controlled by single recessive gene which has pleiotropic effect, indicating that chalky seeds should be GMS seeds in segregating populations. Chalky seeds showed the same shape and size as normal seeds. However, starch composition of central part of endosperm was looser and shape of starch granules was rounder compared with normal endosperm, resulting in significantly lower grain weight, absolute density and grain hardness in chalky grains than in normal ones. Amylose content and gel consistency of chalky grains were much lower and harder, respectively. Male sterile plants showed much shorter plant height, poorer panicle exsertion and lesser panicle number compared with normal plants. Microspore abortion stage in pollen developmental process was observed as before meiosis. Male sterility of the mutant was stable regadless of temperature and day length. A system breeding hybrid rice using this mutant was discussed, comp ring with other systems utilizing cytoplasmic-genic male sterility(CGMS) and environment sensitive GMS(EGMS). Separation of GMS seeds in mixed seed bulks by specific gravity (1.14∼1.16g / cm3) was successful about 85∼90%. But some mixed normal plants were seemed to be easily removed by the apparent difference in growth characters at seedling stage. The highest natural outcrossing rate of this GMS line was as 17.3 % in a plot treated with 2-row pollinator, I-row GMS, and GA3 + cutting of flag leaf + pollen-scattering by rope.

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Characterization of the Neurospora crassa rcm-1 Mutants (Neurospora crassa rcm-1 돌연변이체의 특성)

  • Kim Sang-Rae;Lee Bheong-Uk
    • Korean Journal of Microbiology
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    • v.41 no.4
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    • pp.246-254
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    • 2005
  • Analysis of the complete genome of Neurospora crassa reveals that at least 19 proteins contain tetratricopeptide repeat (TPR) motifs. One of them shows over $60\%$ homology to Ssn6 of Saccharomyces cerevisiae, a universal repressor that mediates repression of genes involved in various cellular processes. Mutant strains generated by RIP (repeat-induced point mutation) process showed four distinctive vegetative growth patterns and slow growth in various rates. Firstly, a mutant showed denser mycelial growth, yellow, csp, and looked like ropy mutant. Secondly, slower growth, dense mycelial, and conidial phenotype. Thirdly, extremely slower growth and aconidial. And finally, flat, tittle aerial hyphae, acon, and similar with a rco-1 RIP mutant. They are all male-fertile, yet female-sterile and produced little or no perithecium. It seems that various phenotypes were occurred depending upon mostly likely, the degree of RIP. These results indicate that this gene may be involved in several cellular possess during vegetative growth, and asexual and sexual development. Therefore it is pleiotropic. Sequence analysis of cDNA shows that it encodes a putative 102 kDa protein composed of 917 amino acids, and has six introns. It is designated rcm-1 (regulation of conidiation and morphology).

Effect of High-fat Diet on Peritoneal Macrophage Immunocompetence in C57/BL6 Mice (고지방 식이가 C57/BL6생쥐의 복강대식세포 면역능력에 미치는 영향)

  • Jeong, Jun-Hyun;Park, Hee-Geun;Lee, Wang-Lok
    • Journal of Life Science
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    • v.23 no.6
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    • pp.779-788
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    • 2013
  • This study was to investigate the effect of high-fat diet on macrophage immunocompetence in C57BL/6 mice. C57BL/6 male mice (4 weeks aged, n=16) were divided into two groups. HD groups fed high-fat diet (45% of fat) and ND groups fed chow diet (10% of fat). Peritoneal macrophages were obtained from each mouse intra-peritoneal by sterile lavage method. Macrophage were stimulated with $1{\mu}g/ml$ of lipopolysaccharide (LPS) for 24 hr. Body weight was significantly increased by high-fat diet. Macrophage phagocytosis of HD was significantly lower than that of ND. After 24 hr of LPS stimulation, NO, IL-$1{\beta}$ and IFN-${\gamma}$ production of HD were significantly lower than those of ND. There were no significant differences in the production of TNF-${\alpha}$ and IL-12 between HD and ND. These findings suggest that high fat diet-induced obesity is associated with decreased Immunocompetence and antigen-stimulated sensitivity of peritoneal macrophage, and lower production of NO, IL-$1{\beta}$ and IFN-${\gamma}$ may contribute to these changes.

Development of the Greenbottle Blowfly, Lucilia sericata, under Different Temperatures (온도별 구리금파리(Lucilia sericata)의 발육)

  • Kim, Hyeon-Cheol;Kim, Soo-Jung;Yun, Ji-Eun;Jo, Tae-Ho;Choi, Byeong-Reol;Park, Chung-Gyoo
    • Korean journal of applied entomology
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    • v.46 no.1 s.145
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    • pp.141-145
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    • 2007
  • Maggot therapy (MT) has been re-introduced for non-healing wounds such as pressure sore, diabetic and necrotic ulcers, as well as infected surgical wounds, burn, and trauma injuries since early 1990s. For the production of sterile maggot of proper developmental stage, Lucilia sericata were studied on developmental periods of immature stages on liver agar medium under six different temperatures such as 15.4, 20.6, 22.5, 26.2, 29.1, and $33.0^{\circ}C$, and adult longevity and egg Production under $29^{\circ}C$. The periods of eggs and larvae of the 1st and the 2nd instars were shortest at $33^{\circ}C$ by 9.0, 14.0 and 18.6 hours, respectively. The periods of the 3rd instar larvae and pupae were shortest at $29.1^{\circ}C$ by 285.0 and 171.0 hours, respectively. Developmental zero point and total effective temperature far the development of each stage were calculated based on the developmental periods. Adults of both female and male lived more than one month. Females laid an average of 338.5 eggs through 2.7 times of egg laying throughout her lifetime.

Predicting Factors of Roseola Infantum Infected with Human Herpesvirus 6 from Urinary Tract Infection

  • Ko, Hong-Ryul;Shin, Son Moon;Park, Sung Won
    • Childhood Kidney Diseases
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    • v.20 no.2
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    • pp.69-73
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    • 2016
  • Purpose: The aim of this study was to compare the clinical and laboratory features of infants with roseola infantum due to human herpesvirus 6 (HHV6) infection and those with urinary tract infection (UTI). Methods: We retrospectively reviewed the medical records of children who were hospitalized at Cheil General Hospital and Women's Health Care Center, College of Medicine, Dankook University, and diagnosed as having HHV6 infection or UTI. Results: Among the infants admitted between September 2014 and May 2016, 92 (male, 45 and female, 47) were included in the study and divided into a HHV6 infection group (n=50) and a UTI group (n=42). The relative risk of UTI compared with that of HHV6 infection increased with pyuria (P<0.001), increased with leukocytosis (mean white blood cell [WBC] count, $15,048{\pm}5,756/mm^3$ vs $87,916{\pm}54,056/mm^3$; P<0.001), increased with C-reactive protein (CRP) level ($4.89{\pm}4.85 mg/dL$ vs $1.04{\pm}1.76mg/dL$; P<0.001), and younger age ($6.3{\pm}3.2months$ vs $18.3{\pm}12.6months$; P<0.001). The relative risk of HHV6 infection compared with that of UTI increased with fever duration ($4.3{\pm}1.7days$ vs $2.8{\pm}1.7days$; P<0.001) and decreased with platelet (PLT) count ($373{\pm}94{\times}10^3/mm^3$ vs $229{\pm}90{\times}10^3/mm^3$; P<0.001). No significant differences were found between the HHV6 groups according to the presence or absence of pyuria. Conclusion: Pyuria, age, fever duration, WBC count, CRP level, and PLT count were the differentiating factors of HHV6 infection from UTI. However, sterile pyuria can occur in children with HHV6 infection. In the presence of pyuria, CRP level and PLT count were the strong predictors of UTI compared with HHV6.

Characteristics Related to Photosynthesis and Grain Yield of $F_1$ Hybrid Rice (벼 1대잡종의 광합성 및 수량 관련 특성)

  • Lee, Deog-Bae;Kwon, Tae-Oh;Lee, Jae-Kil;Park, Keon-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.4
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    • pp.420-427
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    • 1995
  • This study was carried out to investigate heterosis in photosynthetic characteristics and yield of F$_1$ hybrid rice using genetic cytoplasmic male sterile lines. Two F$_1$ hybrid rices, HR7028A/Yongmoonbyeo and IR54756A/Yongmoonbyeo, showed heterobeltiosis in dry weight and leaf area at the heading date and at the 20 day after heading. HR7028A/Yongmoonbyeo showed heterobeltiosis and IR54756A/Yongmoonbyeo showed heterosis in chlorophyll content and RuDPCase activity in flag leaf, T-sugar and starch content in stem, and $CO_2$ fixation at the 20 day after heading as well as at the heading date. HR7028A/Yongmoonbyeo showed heterobeltiosis in number of panicles and grains per panicle, and IR54756A/Yongmoonbyeo had heterobeltiosis in number of panicles per plant. As results, heterobeltiosis of HR7028A/Yongmoonbyeo in yield was 11% and that of IR54756A/Yongmoonbyeo was 4%.

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