• The Korean Journal of Malacology
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• v.12 no.1
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• pp.47-51
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• 1996

Radix auricularia coreana, the intermediate host of Fasciola gigantica, is the most common pulmonate snail in Korea, This species is often found intermingled with Austropeplea ollula, the intermediate host of F. hepatica, in their natural habitats. In the present study. the life history of Radix auricularia coreana was examined under three different laboratory conditions. Egg-masses were taken from the field-collected adult R. auricularia coreana and incubated in the temperature ramges of 22-26$^{\circ}C$. The hatching began after 11 days from spawned eggs, and complete hatching took about 12 days. The hatching rate was about 88%. The juvenile snails were cultured at three different laboratory conditions. When the juvenile snails were cultured in the aquarium fed on lettuce leaves at 22-26$^{\circ}C$, the snails reached 20 mm in shell length at 86 days after hatching. The bottom of each aquarium was filled up with washed sand(1.5 cm) and decomposing ark shells were put on the sand. The aquarium was then filled with four litres of distilled water and continuously aerated. Most of snails (93%) survived until the experimenta period. The dggs are laid in 40 days after hatching; the averge number of eggs per egg-mass was 40.8.

• The Korean Journal of Malacology
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• v.22 no.2
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• pp.143-150
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• 2006

The gonad index, condition index, reproductive cycle and spawning of the pen shell Atrina (Servatrina) pectinata were investigated using samples from the subtidal zone of Nokdo on the Boryeong coastal waters of Korea. Samples were collected monthly by SCUBA divers for one year from January to December, 2001. A. (Servatrina) pectinata is dioecious and oviparous. The spawning season of this species occurred once a year from June to August, with the main spawning occurring between June and July when the seawater temperature was around $20^{\circ}C$. Ripe oocytes were about 60-65 ${\mu}m$ in diameter. The reproductive cycle of this species could be classified into five successive stages; early active stage (November to March), late active stage (February to May), ripe stage (April to July), partially spawned stage (June to August), and spent/inactive stage (August to October). Monthly changes in the gonad index reached a maximum (4.6) in May (ripe stage), thereafter, the GI values gradually decreased from June to August when spawning occurred continuously. Therefore, monthly changes in the GI values showed a similar pattern to the gonadal phase. The condition index (CI) of the meat part without the posterior adductor muscle reached the maximum in June (ripe and partially spawned stage) and the minimum in September (spent/inactive stage), Accordingly, monthly changes in the condition indice of the meat part without the posterior adductor muscle coincided with the gonadal phases.

• The Korean Journal of Malacology
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• v.22 no.2
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• pp.151-155
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• 2006

Proportion of Tegillarca granosa surviving after 2-6 hrs air exposure with 12 hrs interval at $20^{\circ}C$ and $28^{\circ}C$ for 20 days showed 85-100%, 80-100%, respectively. The survival rate was somewhat lower at high temperature but not significant (p < 0.05). Subsequent exposures for 7-9 days showed survival rate of 8.0-24.1% at $20^{\circ}C$ and $28^{\circ}C$. Oxygen consumption rates and filtration rates were significantly higher for 4 to 6 hrs exposures, compared with the preceding exposures. On the other hand, at $28^{\circ}C$, oxygen consumption rates in adult granular ark for 6 hrs exposure during 20 days had significantly decreased. Filtration rates in study groups increased a little over extended period of exposure, compared with those in control groups, and were similar irrelevant to the time of exposure and size of experimental animals. It is concluded, in view of their viability and oxygen consumption rate during air exposure, that ark shells have quite a high resistance to air exposure with their limited range of responses.

• The Korean Journal of Malacology
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• v.22 no.2
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• pp.115-124
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• 2006

Gonad index, spermatogenesis and the reproductive cycle of Meretrix petechialis were investigated by cytological, histological observations. Monthly changes in the gonad index coincides the gonadal development. The morphology of the spermatozoon had a primitive type and is similar to that of other bivalves having a short mid-piece with five to six mitochondria surrounding the centrioles. The morphology of the sperm nucleus type and the acrosome shape of this species were cylindrical type and cap-like shape, respectively. The spermatozoon was approximately 40-45 ${\mu}m$ in length including the sperm nucleus length (about 1.50 ${\mu}m$), acrosome length (0.60 ${\mu}m$) and tail flagellum. The axoneme of the tail flagellum consisted of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail showed 9 + 2 microtubular arrangement. The spawning period was from June to September and the main spawning occurred from July to August when seawater temperatures were higher than $20^{\circ}C$. The reproductive cycle of this species could be categorized into five successive stages: early active stage (February to March), late active stage (February to May), ripe stage (April to July), partially spawned stage (June to September), and spent/inactive stage (September to February).

• The Korean Journal of Malacology
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• v.22 no.1 s.35
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• pp.51-61
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• 2006

Oogenesis and the reproductive cycle in female Ruditapes philippinarum were investigated by cytological and histological observations. R. philippinarum is dioecious and oviparous. During vitellogenesis, the Golgi complex, glycogen particles and mitochondria were involved in the formation of lipid droplets and lipid granules in the cytoplasm of the early vitellogenic oocyte. In the late vitellogenic oocyte, cortical granules, the endoplasmic reticulum, and mitochondria were involved in the formation of proteid yolk granules in the cytoplasm. At this time, exogenous lipid granular substance and glycogen particles in the germinal epithelium passed into the oocyte through the microvilli of the vitelline envelope. The spawning period was once a year between early June and early October, and the main spawning occurred between July and August when seawater temperature was approximately $20^{\circ}C$. The reproductive cycle of this species can be categorized into five successive stages: early active stage (January to March), late active stage (February to May), ripe stage (April to August), partially spawned stage (May to October), and spent/inactive stage (August to February). Percentages of female clams at frst sexual maturity of 15.1-20.0 mm in shell length were 52.6% (50% of the rate of group maturity was 17.83 mm in length), and 100% for the clams > 25.1 mm.

• The Korean Journal of Malacology
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• v.22 no.1 s.35
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• pp.1-11
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• 2006

The gonadosomatic index (GSI), oogenesis and reproductive cycle in female Neptunea (Barbitonia) afhritica cumingii were investigated by light and electron microscope observations. In the early vitellogenic oocyte, the Golgi complex and mitochondria were involved in the formation of glycogen, lipid droplets and yolk granules. Late in the vitellogenic oocytes, the rough endoplasmic reticulum and multivesicular bodies were involved in the formation of proteid yolk granules in cytoplasm. In particular, compared with the results of other gastropods, it showed a different result that appearances of cortical granules at the cortical layer and microvilli on the vitelline envelope, which is associated with heterosynthetic vitellogenesis, were not observed in vitellogenic oocytes during oogenesis. A mature yolk granule was composed of three components: main body (central core), superficial layer, and the limiting membrane. Monthly changes in the gonadosomatic index in females studied in 2004 and 2005 were closely associated with ovarian developmental phases. Spawning occurred between May and August in 2004 and 2005 and the main spawning occurred between June and July when the seawater temperature rose to approximately $18-23^{\circ}C$. The female reproductive cycle can be classified into five successive stages: early active stage (September to October), late active stage (November to February), ripe stage (February to June), partially spawned stage (May to August), and recovery stage (June to August).

• The Korean Journal of Malacology
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• v.9 no.1
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• pp.39-45
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• 1993

한국산 부산입술대고둥의 지역 집단간에 있어 유전적 변이, 종내 유연관계를 밝히기 위하여 애월, 세화, 삼척, 통도사, 거문도 및 여수 지역에서 채집한 총 180개체를 대상으로 전기영동을 실시하였고 여기서 나타난 16개의 유전자를 분석하였다. 각 집단의 유전전 변이 정도를 조사한 결과 전 집단의 평균 대립인자 수 (A)는 1.528이고, 평균 다형형의 빈도(P)는 45.5%이며, 평균이형접합자(H$_{D}$)는 0.205로 나타났다. 이중 삼척집단의 대립인자 수는 1.46으로 가장 낮고, 애월집단의 다형형의 빈도는 28.58%, 통도사의집단은 57.14%로 가장 낮은 빈도를 나타냈다. 각 집단간 유전적 유연관계를 분석한 결과 6개 집단간의 평균 유전적 근연치는 S=0.716이었고, 애월과 삼척집단은 0.903으로 가장 가까운 유전적 유연관계를 4보여주고 있다. 여수집단과 삼척집단의 유전적 유연관계는 0.562로 가장 낮은 값을 나타냈다. Nei의 공식에 따라 각 집단의 유전적 차이치(D)를 계산한 결과 평균D값은 0.267이며, 이중 애월과 삼척집단이 0.067로 가장 가깝게 나타났고 이는 Roger에 의한 유전적 근연관계와 유사한 양상을 보였다.

• The Korean Journal of Malacology
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• v.9 no.1
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• pp.46-62
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• 1993

Six species of freshx\water Unionidae mussels in Korea, Anodonta arcaeformis flavotincta, A. woodiana, Unio douglasiae, U. douglasiae sinuloatus, Lamprotula gottschei, Lanceolaria acorohyncha were compared in regard to the various taxonomical characteristics of ultra structures of shells and glochidia. The results obtained in this study are summarized as follows;the orter demibranchs of all the females of six species have served as marsupial(gravid)pouch. However, all the foru demibranchs were used as the marsupium in some female individuals of L. gottschei. The glochidia of A. arcaeformis flavotincta, A. woodiana and L. gottschei were separated one by one with the mucous strings and U. douglasiae sinuolatus were round-subtriangular in shape, and those of L. gottschei were subelliptical in shape. Each of the glochidial shell valves of six species had a hook studded with many spines on the superior face on valve. The glochidial shell valve had two or three rows of large spines; A. arcaeformis flavotincta, A. woodiana, and L. gottschei had two rows and three rows in U. douglasiae, U. douglasiae sinuolatus, and L. acrorhyncha. The numbers of sensory hairs of A. arcaeformis flavotincta and A. woodiana were all eight, those of U. douglasiae, U. douglasiae sinuolatus and L. acrorhyncha were six. and that of L. gottschei was four. The external and internal surfaces of the glochidial shell valves of A. A. arcadformis flavotincta, A. woodiana, and L. gottschei had foveolate and psilate in U. douglaseae, U. douglasiae sinuolatus and L. acrorhyncha.

• The Korean Journal of Malacology
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• v.25 no.3
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• pp.231-236
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• 2009

This study describes biological environmental characteristics in natural habitats of Haliotis diversicolor in the coastal area of Sungsanpo located in the eastern coast of Jeju-do, Korea. The field survey was monthly conducted from May 2006 to February 2007. H. diversicolor, sea urchin, turban shell and marine algae were collected by two divers using SCUBA. The species composition of demersal fishes were seasonally investigated by fish traps in 2007. During the study period, more than 67% of H. diversicolor and sea urchin were found in the depth shallower than 5 m, and more than 64% of turban shell were found in the depth deeper than 3 m. The dominant algal species in May were generally Undaria pinnatifida, Ecklonia cava, Sargassum. spp. and coralline algae. Most of demersal fishes collected by the fish traps were wrasses which have been reported as a major predator of young abalone.

• The Korean Journal of Malacology
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• v.25 no.3
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• pp.179-187
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• 2009

The effects of water temperature and salinity on embryonic development and spat survival of mussel Musculista senhousia were investigated. Embryos were incubated in water ranging from 0 to $35^{\circ}C$ and with salinity from 5‰ to 40‰. Mussel spat were tested in water from 0 to $40^{\circ}C$ and with salinity from 0‰ to 100‰. The optimal conditions for mussel embryos were $20-25^{\circ}C$ and salinity from 25‰ to 35‰, based on Within this temperature range, higher temperatures correponded to a shorter duration of the embryonic period. Optimisation of mussel spat survival was at $25-35^{\circ}C$ and salinity from 30‰ to 40‰; both values are higher than those for embryo, which hinted M. senhousia embryos are more vulnerable than spat. Temperatures below $15^{\circ}C$ were lethal for embryos, making temperature a feasible method with which to control the large population of M. senhousia in ark-shell farm during its spawning period.