• Journal of Embryo Transfer
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• v.25 no.4
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• pp.281-286
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• 2010

This study was to evaluate the protein profile of seminal plasma using 2-DE in Hanwoo. Seminal plasma was harvested from five mature Hanwoo, and seminal plasma protein was extracted by M-PER Mammalian Protein Extraction Reagent. Proteins were refined by clean-up kit and quantified by Bradford method until total protein was $300\;{\mu}l$. Immobilized pH gradient (IPG) strip was used 18 cm and 3~11 NL. SDS-PAGE was used 12% acrylamide gel. Each gels were visualized by comassie brilliant blue and silver staining. These spots were analyzed by MALDI-TOF MS and searched on NCBInr. The result, 20 proteins of 36 protein spots were searched through peptide sequencing on the NCBInr. 8 proteins profiled by 2-DE were proved through previous bovine studies and the name of each protein was albumin, nucleobindin, clusterin, TIMP-2, spermadhesin Z13, spermadhesin-1 and BSP proteins (BSP 30 kDa and BSP A1/A2). 12 new proteins were ATP synthase, protein MAK16 homolog, Transmembrane protein 214, E3 ubiquitin-protein ligase BRE1A, dual serine/threonine and tyrosine protein kinase, tissue factor pathway inhibitor 2, alpha-actinin-4, RUN domain-containing protein 3B, catenin alpha-1, protein-glutamine gamma-glutamyltransferase 2, plakophilin-1 and inter-alpha-trypsin inhibitor heavy chain H1 has not been previously described in the bovine seminal plasma study. These proteins may be contribute to define the type of proteins affecting fertility of male and improve the fertilizing ability of semen in Hanwoo.

• Journal of Digital Contents Society
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• v.19 no.7
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• pp.1263-1270
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• 2018

We investigated the e-learning platforms for higher education in developing countries. First, we introduced e-learning environmental factors such as ICT technology as well as ASEAN Cyber University (ACU) project for developing countries. And then, we presented Cambodian case as an example of e-learning platform for developing countries. We analyzed the national education policy and ICT environment as well as the demands and obstacles of e-learning in Cambodia. We derived e-learning strategies and plans based on the analysis results, and finally proposed e-learning platform C-MOOC Net. In order to verify the proposed scheme, we developed prototype of C-MOOC Net system which is implemented based on open software. As a result, we have met the requirement of networking the C-MOOC hub, supporting localization of local language and personalization of favorite courses respectively.

• Preventive Nutrition and Food Science
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• v.16 no.3
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• pp.210-216
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• 2011

Capsaicin is a pungent component of red pepper, which is widely consumed as food adjuncts. The present study was performed to investigate anti-obesity effects of capsaicin in diet-induced obese rats. Male Sprague-Dawley rats (n=14) were fed with a high-fat diet (Control) or high-fat diet containing 0.016% capsaicin (w/w) (Capsaicin) for 8 weeks. The final body weight and the mass of white adipose tissue were significantly lower in capsaicin supplemented group compared to control. Dietary capsaicin ameliorated lipid profiles with decrease in the plasma concentrations of triglycerides and total cholesterol, and decrease in the levels of total lipids and triglycerides in the liver. Activity of glycerol-3-phosphate dehydrogenase (GPDH), an indicator of triglyceride biosynthesis in white adipose tissue, decreased by 35% in the group supplemented with capsaicin. However, consumption of capsaicin increased the expression of uncoupling protein 2 (UCP2) in white adipose tissue, which is related to energy consumption. Our data suggests that capsaicin may reduce body weight and fat accumulation in high fat diet-induced obese rats. These effects may be mediated, at least partially, by the upregulation of UCP2 gene expression and its ability to inhibit GPDH activity.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.5
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• pp.608-613
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• 2016

Arbuscular mycorrhizal fungi (AMF) spore propagation and long term maintenance is still a complicated technique for farmers. The use of AMF for their ability to promote plant growth and protect plants against pathogen attack and environmental stresses demands AMF propagation for large scale application. This study aimed to propagate AMF spores by trap culture technique and assess their ability to propagate with different host plants in a continuous plant cycle. Mycorrhizal inoculation by trap culture in maize resulted in longer shoots and roots than sudangrass plants. Increase in dry weight with higher percentage also was observed for maize plants. After first and second plant cycle, maize plants had the higher percentage of mycorrhizal response in terms of colonization and arbuscules than sudangrass. Maximum in spore count also achieved in the pots of maize plants. The results show that maize plant is more suitable host plant for AMF spore propagation and trap culture technique can be used effectively to maintain the AMF culture for long time.

• Journal of Sasang Constitutional Medicine
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• v.16 no.2
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• pp.44-51
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• 2004

1. Objectives This paper is written for clear understanding on the concepts of 'Deep adherence of the Nature' and 'Sudden explosion of the Emotion' and for intensive comprehension of Mechanism of 'The Exterior Disease' and 'The Interior Disease' by The Nature and the Emotion. 2. Methods The study mainly based on "The exterior disease injured by the Nature, the interior disease injured by the Emotion" and deals with 'Seongmyungron, Sadanron, Hwarkchungron and Jangburon'. 3. Results and conclusions The conclusions as follows (1) Exterior Disease in each constitution breaks out by deep adherence of the Nature. Interior Disease brings out with sudden explosion of the Emotion. (2) 'Deep adherence of the Nature' is the state that the Ear, Eye, Nose, Mouth taking care of environmental frames can't work enoughly because of wrong minds on the Jaw, Breast, Umbilicus, Abdment. 'Sudden explosion of the Emotion' is the state that the Lung, Spleen, Liver, Kidney doing human affairs doesn't do appropriately for each constitution due to negative minds on the Head, Shoulder, Waist, Hip. (3) The result that the Ear, Eye, Nose, Mouth doesn't extract the clear energy from 'Jin, Go, You, Yaek sea' so they are lack of 'Shin, Qi, Hyul, Jeong' causes exterior disease in each constitution. (4) In each constitution, Interior disease results from that the Lung, Spleen, Liver, Kidney fails to bring on the clear juice from 'Lee, Mak, Hyul, Jeong sea', so shortage of 'Jin, go, You, Yaek' results in it.

• Journal of Pharmacopuncture
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• v.19 no.3
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• pp.231-238
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• 2016

Objectives: This study used repeated intravenous injections of Saeng Maek San (SMS) injection in Sprague-Dawley (SD) rats to assess the toxicity and the stability of SMS. Methods: Six-week-old male and female SD rats reared by Orient bio Inc were chosen for this pilot study. They were randomly split into four groups: Group 1 (G1), the control group (0.3 mL of normal saline solution/day/animal), and Groups 2, 3 and 4 (G2, G3 and G4), the experimental groups (0.1, 0.2 and 0.3 mL/day/animal of SMS), respectively. Each animal received an intravenous injection of SMS once a day for four weeks. Clinical signs, body weight changes, and food consumption were monitored during the observation period, and urinalysis and hematology were conducted after four weeks of SMS or saline administration. Results: No deaths occurred in any of the four groups during the observation period. Compared to the control group, male and female rats in groups 3 and 4 (0.2 and 0.3 mL/animal/day) showed hemoglobinuria, but the low-dosage group (G2, 0.1 mL/animal/day) showed no significant changes in the clinical signs test. No significant changes due to SMS were observed in the experimental groups regarding body weight changes, food consumption urinalysis, or hematology. Conclusion: During this study, no mortalities were observed in any of the experimental groups and no hemoglobinuria was observed in the low dosage group (0.1 mL/animal/day) while it was intermittently observed in groups 3 and 4 (0.2 and 0.3 mL/animal/day). Thus, we suggest that the no-observed adverse-effect level (NOAEL) is 0.1 mL/animal/day in male and female SD rats.

• Journal of the Korean Society of Hazard Mitigation
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• v.8 no.1
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• pp.117-123
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• 2008

In this study, PHABSIM which is a sample for 1D physical habitat and River2D, which is a sample for 2D physical habitat were applied to the main streams of Han River in order to calculate an optimum flow considering the habitats of fishes in determining the instream flow. Moreover, the Weighted Usable Area (WUA) of the two samples in each growth step (adults and spawning) of the target fish type was compared and reviewed. The optimal flow value was calculated by considering the conditions for inhabiting fishes. As a result of the correlation analysis for WUA from 1D and 2D samples was 0.87 to 0.99. The optimum flow considering the conditions of inhabiting fishes showed insignificant difference of $3m^3/s\;to\;5m^3/s$ with the exception of adults in Moon-Mak and spawning in Dal-Chun.

• Journal of Animal Science and Technology
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• v.56 no.7
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• pp.23.1-23.7
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• 2014

Background: Scanning of the genome for selection signatures between breeds may play important role in understanding the underlie causes for observable phenotypic variations. The discovery of high density single nucleotide polymorphisms (SNPs) provide a useful starting point to perform genome-wide scan in pig populations in order to identify loci/candidate genes underlie phenotypic variation in pig breeds and facilitate genetic improvement programs. However, prior to this study genomic region under selection in commercially selected Berkshire and Korean native pig breeds has never been detected using high density SNP markers. To this end, we have genotyped 45 animals using Porcine SNP60 chip to detect selection signatures in the genome of the two breeds by using the $F_{ST}$ approach. Results: In the comparison of Berkshire and KNP breeds using the FDIST approach, a total of 1108 outlier loci (3.48%) were significantly different from zero at 99% confidence level with 870 of the outlier SNPs displaying high level of genetic differentiation ($F_{ST}{\geq}0.490$). The identified candidate genes were involved in a wide array of biological processes and molecular functions. Results revealed that 19 candidate genes were enriched in phosphate metabolism (GO: 0006796; ADCK1, ACYP1, CAMK2D, CDK13, CDK13, ERN1, GALK2, INPP1; MAK, MAP2K5, MAP3K1, MAPK14, P14KB, PIK3C3, PRKC1, PTPRK, RNASEL, THBS1, BRAF, VRK1). We have identified a set of candidate genes under selection and have known to be involved in growth, size and pork quality (CART, AGL, CF7L2, MAP2K5, DLK1, GLI3, CA3 and MC3R), ear morphology and size (HMGA2 and SOX5) stress response (ATF2, MSRB3, TMTC3 and SCAF8) and immune response (HCST and RYR1). Conclusions: Some of the genes may be used to facilitate genetic improvement programs. Our results also provide insights for better understanding of the process and influence of breed development on the pattern of genetic variations.

• The Korean Journal of Food And Nutrition
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• v.30 no.5
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• pp.900-907
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• 2017

This study was performed to investigate the body fat-lowering effect of garlic powder in peroxisome proliferator-activated receptor ${\gamma}$ coactivator-$1{\alpha}$(PGC-$1{\alpha}$)-luciferase transgenic mice (TG). In this study, we generated transgenic mice with a PGC-$1{\alpha}$ promoter (-970/+412 bp) containing luciferase as a reporter gene. Mice were fed a 45% high-fat diet for 8 weeks to induce obesity. Subsequently, mice were maintained on either a high-fat control diet (CON), or high-fat diets supplemented with 2% (GP2) or 5% (GP5) garlic powder for an additional 8 weeks. Dietary garlic powder reduced the body weight in the GP2 and GP5 groups, compared to the CON group. Furthermore, garlic supplementation significantly decreased the plasma levels of triglycerides, total cholesterol, and leptin in the GP5 group, compared to the CON group. Specifically, luciferase activity in liver, white adipose tissue (WAT), and brown adipose tissue (BAT) was increased by garlic supplementation in a dose-dependent manner. These results suggest that the body fat-lowering effect of garlic powder might be related to PGC-$1{\alpha}$ by the increase in luciferase activity in liver, WAT, and BAT. Furthermore, transgenic mice might be useful for evaluating the body fat-lowering effect of various health functional foods.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.5
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• pp.125-130
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• 2009

Storage function method which considers the non-linearity of the relationship between rainfall and runoff has been frequently used to predict runoff in a basin and a flood pattern. However, it is time-consuming to estimate appropriate parameters of every basin and rainfall event, which requires the empirical parameter equation applicable in Korea. In this study, multiple regression analysis is used to develop empirical equations to estimate parameters of Storage Function method using basin characteristics. The basin area, maximum stream length, and stream slope are considered as the basin characteristics as the result of the regression analysis. Collinearity is removed and trial-and-error method is used to choose the most descriptive parameters to the dependent variables in Han River basin which is divided into 30 subbasins. The developed equations are validated using the rainfall events in MunMak gauging station and named as 'Han River equation'. The equation could provide the useful information about Storage Function method parameter to calculate runoff from a basin and predict river stage.