Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
권호 표지
DOI QR코드

DOI QR Code

Trap Culture Technique for Propagation of Arbuscular Mycorrhizal Fungi using Different Host Plants

  • Selvakumar, Gopal (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Kim, Kiyoon (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Walitang, Denver (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Chanratana, Mak (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Kang, Yeongyeong (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Chung, Bongnam (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Sa, Tongmin (Department of Environmental and Biological Chemistry, Chungbuk National University)
  • Received : 2016.08.23
  • Accepted : 2016.10.27
  • Published : 2016.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

Arbuscular mycorrhizal fungi (AMF) spore propagation and long term maintenance is still a complicated technique for farmers. The use of AMF for their ability to promote plant growth and protect plants against pathogen attack and environmental stresses demands AMF propagation for large scale application. This study aimed to propagate AMF spores by trap culture technique and assess their ability to propagate with different host plants in a continuous plant cycle. Mycorrhizal inoculation by trap culture in maize resulted in longer shoots and roots than sudangrass plants. Increase in dry weight with higher percentage also was observed for maize plants. After first and second plant cycle, maize plants had the higher percentage of mycorrhizal response in terms of colonization and arbuscules than sudangrass. Maximum in spore count also achieved in the pots of maize plants. The results show that maize plant is more suitable host plant for AMF spore propagation and trap culture technique can be used effectively to maintain the AMF culture for long time.

Keywords

References

  1. Abdel Latef, A.A.F. and H. Chaoxing. 2011. Effect of arbuscular mycorrhizal fungi on growth, mineral nutrition, antioxidant enzymes activity and fruit yield of tomato grown under salinity stress. Sci. Hortic. 127:228-233. https://doi.org/10.1016/j.scienta.2010.09.020
  2. Asrar, A.W.A. and K.M. Elhindi. 2011. Alleviation of drought stress of marigold (Tagetes erecta) plants by using arbuscular mycorrhizal fungi. Saudi J Biol Sci. 18:93-98. https://doi.org/10.1016/j.sjbs.2010.06.007
  3. Brundrett, M.C., L.K. Abbott, and D.A. Jasper. 1999. Glomalean mycorrhizal fungi from tropical Australia I. Comparison of the effectiveness and specificity of different isolation procedures. Mycorrhiza 8:305-314. https://doi.org/10.1007/s005720050251
  4. de Souza, F.A. and S. Declerck. 2003. Mycelium development and architecture, and spore production of Scutellospora reticulate in monoxenic culture with Ri T-DNA transformed carrot roots. Mycologia 95:1004-1012. https://doi.org/10.2307/3761908
  5. Douds, D.D. Jr., G. Nagahashi, and P.R. Hepperly. 2010. On-farm production of inoculum of indigenous arbuscular mycorrhizal fungi and assessment of diluents of compost for inoculum production. Bioresour. Technol. 101:2326-2330. https://doi.org/10.1016/j.biortech.2009.11.071
  6. Douds, D.D. Jr., G. Nagahashi, P.E. Pfeffer, C. Reider, and W.M. Kayser. 2006. On-farm production of AM fungus inoculum in mixtures of compost and vermiculite. Bioresour. Technol. 97:809-818. https://doi.org/10.1016/j.biortech.2005.04.015
  7. Fracchia, S., A. Menendez, A. Godeas, and J.A. Ocampo. 2001. A method to obtain monosporic cultures of arbuscular mycorrhizal fungi. Soil Biol. Biochem. 33:1283-1285. https://doi.org/10.1016/S0038-0717(01)00014-1
  8. Gaur, A. and A. Adholeya. 2002. Arbuscular-mycorrhizal inoculation of five tropical fodder crops and inoculum production in marginal soil amended with organic matter. Biol. Fertil. Soils. 35:214-218. https://doi.org/10.1007/s00374-002-0457-5
  9. Hajiboland, R., N. Aliasgharzadeh, S.F. Laiegh, and C. Poschenrieder. 2010. Colonization with arbuscular mycorrhizal fungi improves salinity tolerance of tomato (Solanum lycopersicum L.) plants. Plant Soil. 331:313-327. https://doi.org/10.1007/s11104-009-0255-z
  10. Jansa, J., A. Mozafar, T. Anken, R. Ruh, I.R. Sanders, and E. Frossard. 2002. Diversity and structure of AMF communities as affected by tillage in a temperate soil. Mycorrhiza. 12:225-234. https://doi.org/10.1007/s00572-002-0163-z
  11. Johansson, J.F., L.R. Paul, and R.D. Finlay. 2004. Microbial interactions in the mycorrhizosphere and their significance for sustainable agriculture. FEMS Microbiol. Ecol. 48:1-13.
  12. Kaya, C., M. Ashraf, O. Sonmez, S. Aydemir, A.L. Tuna, and M.A. Cullu. 2009. The influence of arbuscular mycorrhizal colonization on key growth parameters and fruit yield of pepper plants grown at high salinity. Sci. Hortic. 121:1-6. https://doi.org/10.1016/j.scienta.2009.01.001
  13. Lee, Y., R. Krishnamoorthy, G. Selvakumar, K.Y. Kim, and T.M. Sa. 2015. Alleviation of salt stress in maize plant by co-inoculation of arbuscular mycorrhizal fungi and Methylobacterium oryzae CBMB20. J. Korean Soc. Appl. Biol. Chem. 58:533-540. https://doi.org/10.1007/s13765-015-0072-4
  14. Millner, P.D. and D.G. Kitt. 1992. The Beltsville method for soilless production of vesicular-arbuscular mycorrhizal fungi. Mycorrhiza. 2:9-15. https://doi.org/10.1007/BF00206278
  15. Mohammad, A., A.G. Khan, and C. Kuek. 2000. Improved aeroponic culture of inocula of arbuscular mycorrhizal fungi. Mycorrhiza. 2:9-15.
  16. Nair, A., S.P. Kolet, H.V. Thulasiram, and S. Bhargava. 2015. Systemic jasmonic acid modulation in mycorrhizal tomato plants and its role in induced resistance against Alternaria alternate. Plant Biol. 17:625-631. https://doi.org/10.1111/plb.12277
  17. Navarro, A., A. Elia, G. Conversa, P. Campi, and M. Mastrorilli. 2012. Potted mycorrhizal carnation plants and saline stress: growth, quality and nutritional plant responses. Sci. Hortic. 140:131-139. https://doi.org/10.1016/j.scienta.2012.03.016
  18. Phillips, J.M. and D.S. Hayman. 1970. Improved procedure for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Br. Mycol. Soc. 55:159-161.
  19. Schalamuk, S. and M. Cabello. 2010. Arbuscular mycorrhizal fungal propagules from tillage and no-tillage systems: possible effects on Glomeromycota diversity. Mycologia. 102:261-268. https://doi.org/10.3852/08-118
  20. Selvakumar, G., R. Krishnamoorthy, K.Y. Kim, and T.M. Sa. 2016. Propagation technique of arbuscular mycorrhizal fungi isolated from coastal reclamation land. Eur. J. Soil Biol. 74:39-44. https://doi.org/10.1016/j.ejsobi.2016.03.005
  21. Smith, S.E. and D.J. Read. 2008. Mycorrhizal symbiosis, 3rd ed., Academic, London.
  22. Tajini, F., P. Suriyakup, H. Vailhe, J. Jansa, and J.J. Drevon. 2009. Assess suitability of hydroaeroponic culture to establish tripartile symbiosis between different AMF species, beans, and rhizobia. BMC Plant Biol. 9:73. https://doi.org/10.1186/1471-2229-9-73
  23. Trejo-Aguilar, D., L. Lara-Capistran, I.E. Maldonado-Mendoza, R. Zulueta-Rodriguez, W. Sangabriel-Conde, M.E. Mancera-Lopez, S. Negrete-Yankelevich, and I. Barois. 2013. Loss of arbuscular mycorrhizal fungal diversity in trap cultures during long-term subculturing. IMA Fungus. 4:161-167. https://doi.org/10.5598/imafungus.2013.04.02.01
  24. Trouvelot, A., J.L. Kough, and V. Gianinazzi-Pearson. 1986. Mesure du taux de mycorhization VA dun systeme radiculaire. Recherche de methodes destimation ayant une signification fonctionnelle, p. 217-221. In: V. Gianinazzi-Pearson, S. Gianinazzi. (Eds.), Physiological and genetical aspects of mycorrhizae. INRA Press, Paris.
  25. Wang, Y., M. Vestberg, C. Walker, T. Hurme, X. Zhang, and K. Lindstrom. 2008. Diversity and infectivity of arbuscular mycorrhizal fungi in agricultural soils of the Sichuan province of mainland china. Mycorrhiza. 18:59-68. https://doi.org/10.1007/s00572-008-0161-x
  26. Wu, Q.S. and Y.N. Zou. 2009. Arbuscular mycorrhizal symbiosis improves growth and root nutrient status of citrus subjected to salt stress. Sci. Asia. 35:388-391. https://doi.org/10.2306/scienceasia1513-1874.2009.35.388
  27. Wu, Q.S., Y.N. Zou, W. Liu, X.F. Ye, H.F. Zai, and L.J. Zhao. 2010. Alleviation of salt stress in citrus seedlings inoculated with mycorrhiza: changes in leaf antioxidant defense systems. Plant Soil Environ. 56:470-475. https://doi.org/10.17221/54/2010-PSE

Cited by

  1. Assessment of arbuscular mycorrhizal fungal spore density and viability in soil stockpiles of South African opencast coal mines pp.2167-034X, 2018, https://doi.org/10.1080/02571862.2018.1537011