• Title/Summary/Keyword: maize root

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ACC Deaminase Producing Arsenic Tolerant Bacterial Effect on Mitigation of Stress Ethylene Emission in Maize Grown in an Arsenic Polluted Soil

  • Shagol, Charlotte C.;Subramanian, Parthiban;Krishnamoorthy, Ramasamy;Kim, Kiyoon;Lee, Youngwook;Kwak, Chaemin;Sundaram, Suppiah;Shin, Wansik;Sa, Tongmin
    • Korean Journal of Soil Science and Fertilizer
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    • v.47 no.3
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    • pp.213-216
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    • 2014
  • Arsenic is a known hazardous metalloid not only to the animals but also to plants. With high concentrations, it can impede normal plant growth and cause even death of plants at extremely high levels. A known plant response to stress conditions such as toxic levels of metal (loids) is the production of stress ethylene, causing inhibitory effect on root growth in plants. When the effect of various arsenic concentrations was tested to maize plant, the stress ethylene emission proportionately increased with increasing concentration of As(V). The inoculation of two arsenic tolerant bacteria; Pseudomonas grimonti JS126 and Pseudomonas taiwanensis JS238 having respective high and low 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity reduced stress ethylene emission by 59% and 30% in maize grown in arsenic polluted soils. The result suggested the possible use of Pseudomonas grimonti JS126 for phytoremediation of arsenic polluted soils.

Effect of Waterlogging on Growth and Morphological Characteristics of Roots of Maize Inbred Lines (담수 처리에 따른 옥수수 자식 계통의 지상부와 뿌리의 생육 및 형태적 특성)

  • Lee, Jihyun;Shin, Myeong-Na;Jung, Gun-Ho;Kim, Jung Tae;Cha, Jungeun;Shim, Kang-Bo;Lee, Jae-Eun;Son, Beom-Young;Kim, Sang-Gon;Ku, Bon-Il;Lee, Seukki;Jeon, Weon-Tai
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.40 no.4
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    • pp.227-235
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    • 2020
  • This study was conducted to investigate the growth characteristics of the shoot and roots and to analyse the morphological characteristics of roots of waterlogging resistant and susceptible maize inbred lines. Six maize inbred lines were treated with waterlogging for 10 days at V3, and the degree of leaf senescence was evaluated for waterlogging resistance. As a result of waterlogging resistance evaluation, KS85 was the most damaged inbred line with 3.33 senescence leaves and 5.54 degree, and KS141 was the least damaged inbred line with 1.33 senescence leaves and 3 degree. At 20 days after treatment, the effect of waterlogging stress on the shoot dry matter accumulation of KS85 and KS141 were decreased by 86.1% and 77.0%, respectively, compared to the control. Similarly, root dry matter accumulation of KS85 and KS141 were decreased by 77.6% and 65.0%. As a result of SEM photographs of the nodal roots of the two maize inbred lines, the thickness of cortex of KS141 was thicker than that of KS85, and the distortion of the cortex was observed in KS85 at 20 days after waterlogging. It was concluded that the thickness of cortex was related to maize waterlogging resistance.

Transformation of Plant Cells by Gene Transfer : Construction of a Chimeric Gene Containing Deleted Maize Alcohol Dehydrogenase Intron and ${\beta}-Glucuronidase$ Gene and Its Expression in Potato (유전자 도입에 의한 식물세포의 형질전환 : 옥수수 알코올 탈수소효소 유전자의 절단된 인트론 및 ${\beta}-Glucuronidase$ 유전자를 함유하는 키메라 유전자의 제조와 감자에서의 발현)

  • 이광웅
    • Journal of Plant Biology
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    • v.35 no.3
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    • pp.237-245
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    • 1992
  • To understand the properties of the cauliflower mosaic virus (CaMV) 35S promoter and the effect of the deleted maize alcohol dehydrogenase I-S (Adhl-S) intron 1 on the expression of the CaMV $35S{\beta}-glucuronidase$ (GUS) gene in potato (Solanum tuberosum L. cv. Superior), we constructed a chimeric gene and transferred it into potato with Agrobacterium tumefaciens mediated method. The pLS201, a gene transfer vector of 17.7 kilobase pairs, was composed of the CaMV 35S promoter, the 249 base pairs of deleted maize Adhl-S intron 1, the GUS reporter gene, and the kanamycin resistance gene as a selectable marker for transformation. The GUS activity was examined by histochemical and spectrophotometric assay in transformed potato plants. The GUS activity was found primarily around the vascular tissue cells in stem and root. In the spectorophotometric assay, the level of GUS activity of transgenic potato transformed with CaMV 35S/249 bp of intron 1 fragment-GUS (pLS201) was compared with that of potato transformed with CaMV 35S-GUS (pBI121). The quantitative spectrophotometric assay showed that the level of GUS activity in potato transformed with pLS201 was higher in leaf, stem and root by 30-, 34- and 42-fold, respectively than those in potato transformed with pBI121. This results indicate that the inclusion of the deleted maize Adhl-S intron 1 resulted in increament of the GUS gene expression in transgenic potato.potato.

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NUTRIENT CHARACTERISTICS OF SOME LOCALLY AVAILABLE FEED RESOURCES IN FIJI

  • Ochetim, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.5 no.1
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    • pp.97-100
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    • 1992
  • Locally available milk mix, coconut meal, cassava root meal, cassava leaf meal, maize, meat meal, fish meal, rice bran, rice polishings, wallow, molasses and coral sand in Fiji were analysed for proximate components, gross energy and mineral contents. The results obtained indicated typical and variable amounts of nutrients in these materials. On the basis of their nutrient contents, the potential usefulness and weaknesses of the various feed materials are discussed with specific reference to the formulations of diets for livestock and poultry in Fiji based on these locally available feed materials.

Occurrence of the Root-Knot Nematode species in Vegetable Crops in Souss Region of Morocco

  • Janati, Soukaina;Houari, Abdellah;Wifaya, Ahmed;Essarioui, Adil;Mimouni, Abdelaziz;Hormatallah, Abderrahim;Sbaghi, Mohamed;Dababat, Abdelfattah A.;Mokrini, Fouad
    • The Plant Pathology Journal
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    • v.34 no.4
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    • pp.308-315
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    • 2018
  • Root-knot nematodes 'Meloidogyne spp' are the most destructive group of plant parasitic nematodes causeing serious losses in vegetables crops and this damages worsened when crops grown under greenhouses conditions. In this sutdy, the distribution and characterization of root-knot nematode species collected from the Souss region of Morocco where vegetables crops intensively cultivated were determined by using both morphological and molecular tools. Out of the 110 samples collected from different greenhouses 91 (81.7%) were found to be infested with root-knot nematodes. Thirty-seven populations of root-knot nematodes were morphologically identified based on perineal patterns as well as molecularlly using species-specific primers. The obtained results indicated that Meloidogyne javanica and M. incognita were identified in 86.4% and 13.5% of the total populations, respectively. The lowest incidence of root-knot nematodes (64%) was found in Toussous, whereas the highest frequencies of 100% and 90% were detected in Taddart and Biogra, respectively. As the majority of the samples have been infested with Meloidogyne species; this indicates that there is an urgent need to provide farmers with a proper control strategy.

Research Status for Drought Tolerance in Maize (옥수수 한발 내성에 관한 연구 현황)

  • Kim, Kyung-Hee;Moon, Jun-Cheol;Kim, Jae-Yoon;Kim, Hyo-Chul;Shin, Seung-Ho;Song, Ki-Tae;Lee, Byung-Moo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.4
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    • pp.401-411
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    • 2015
  • Drought stress has detrimental effects on the seedling development, vegetative/ reproductive growth, photosynthesis, root proliferation, anthesis, anthesis-silking interval (ASI), pollination and grain yield in maize. Typically, two weeks before silking through pollination are an important time in maize life. Here we reviewed the effects of drought stress on growth, physiological/ molecular researches for drought tolerance, and breeding to genomics in maize. Drought stress during kernel development increases leaf dying and lodging, decreases grain filling period and grain yield. Physiological factors of drought stress/ effects are water content, water deficits, and water potential. Nowdays molecular marker assisted breeding method is becoming increasingly useful in the improvement of new germplasm with drought stress tolerance.

Analysis of Plant Height, Crop Cover, and Biomass of Forage Maize Grown on Reclaimed Land Using Unmanned Aerial Vehicle Technology

  • Dongho, Lee;Seunghwan, Go;Jonghwa, Park
    • Korean Journal of Remote Sensing
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    • v.39 no.1
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    • pp.47-63
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    • 2023
  • Unmanned aerial vehicle (UAV) and sensor technologies are rapidly developing and being usefully utilized for spatial information-based agricultural management and smart agriculture. Until now, there have been many difficulties in obtaining production information in a timely manner for large-scale agriculture on reclaimed land. However, smart agriculture that utilizes sensors, information technology, and UAV technology and can efficiently manage a large amount of farmland with a small number of people is expected to become more common in the near future. In this study, we evaluated the productivity of forage maize grown on reclaimed land using UAV and sensor-based technologies. This study compared the plant height, vegetation cover ratio, fresh biomass, and dry biomass of maize grown on general farmland and reclaimed land in South Korea. A biomass model was constructed based on plant height, cover ratio, and volume-based biomass using UAV-based images and Farm-Map, and related estimates were obtained. The fresh biomass was estimated with a very precise model (R2 =0.97, root mean square error [RMSE]=3.18 t/ha, normalized RMSE [nRMSE]=8.08%). The estimated dry biomass had a coefficient of determination of 0.86, an RMSE of 1.51 t/ha, and an nRMSE of 12.61%. The average plant height distribution for each field lot was about 0.91 m for reclaimed land and about 1.89 m for general farmland, which was analyzed to be a difference of about 48%. The average proportion of the maize fraction in each field lot was approximately 65% in reclaimed land and 94% in general farmland, showing a difference of about 29%. The average fresh biomass of each reclaimed land field lot was 10 t/ha, which was about 36% lower than that of general farmland (28.1 t/ha). The average dry biomass in each field lot was about 4.22 t/ha in reclaimed land and about 8 t/ha in general farmland, with the reclaimed land having approximately 53% of the dry biomass of the general farmland. Based on these results, UAV and sensor-based images confirmed that it is possible to accurately analyze agricultural information and crop growth conditions in a large area. It is expected that the technology and methods used in this study will be useful for implementing field-smart agriculture in large reclaimed areas.

The Effect of the Colonization of Serratia plymuthica A21-4 in Rhizosphere Soil and Root of Pepper in Different Soil Environment (근권토양의 환경이 고추역병 억제 미생물 Serratia plymuthica A21-4의 고추뿌리와 근권 토양 정착에 미치는 영향)

  • Cao, Pu;Shen, Shun-Shan;Wen, Cai-Yi;Song, Shuang;Park, Chang-Seuk
    • Research in Plant Disease
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    • v.15 no.2
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    • pp.101-105
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    • 2009
  • The biocontrol agent Serratia plymuthica A21-4 was selected and proved as an excellent inhibitor of Phytophthora blight of pepper through in vitro and in vivo experiments in previous studies. To enhance the colonizing density of S. plymuthica A21-4 on plant root and rhizosphere soil, some soil conditions might effect on the colonization of the bacteria were examined. The results obtained from the study indicated that the soils containing more sand were favorable to root colonization of S. plymuthica A21-4. Organic amendment such as 3% maize straw(w/w) was helpful to colonize the bacteria in root and soil. The soil temperature about $20^{\circ}C$, water content around 40%, and soil pH near to neutral or slightly acidic, were optimum condition for the colonization of S. plymuthica A21-4 in the rhizosphere soil and roots of pepper. In addition, existence of indigenous biotic entities was beneficial to the colonization of S. plymuthica A21-4.

Expression of gus and gfp Genes in Ggrlic (Allium sativum L.) Cells Following Particle Bombardment Transformation

  • Lacorte, Cristiano;Barros, Daniella
    • Journal of Plant Biotechnology
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    • v.2 no.3
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    • pp.135-142
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    • 2000
  • The activity of promoter sequences was evaluated in garlic cells using the $\beta$-glucuronidase (GUS) gene as a reporter. Histochemical GUS assay indicated transient GUS activity in leaf, callus and root cells 48 hours after particle bombardment transformation. Quantitative fluorometric assays in extracts of transformed leaves demonstrated that the CsVMV promoter induced the highest level of gene expression, which was, on average, ten fold the level induced by CaMV35S and by the Arabidopsis Act2 promoters and two fold the level expression observed with a construct containing a double CaMV35S plus the untranslated leader sequence from AMV. No activity or very low levels were observed when cells were transformed with plasmids rontaining the typical monocot promoters, Actl, from rice or the Ubi-1, from maize. The green fluorescent protein (GFP) was also tested as a marker gene for garlic transformation. Intense fluorescence was observed in leaf, callus and root cells transformed with a construct containing the gfp gene under control of the CaMV35 Promoter. No fluorescence was detected when the gfp was under control of the Ubi-1 promoter.

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The Density Effect on the Dry Matter Production of Zea mays (옥수수의 건물질성산에 미치는 밀도효과)

  • 이일구
    • Journal of Plant Biology
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    • v.13 no.1
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    • pp.56-64
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    • 1970
  • The present experiments differning from that conducted by agriculturists with the first consideration of food or seed production were performed in order to investigate the density effect on dry matter production in the artified population of maize. Three experimental plots were established and one seed was sown in the first plot, 2 seeds in the second plot and 3 seeds in the third plot, respectively. The space of each ridge was designed with 30cm in width and 50cm in length. The space of each seed was 4cm and the growth amount by density was measured through three times. The results obtained are as follows: 1) The total growth amount per unit area was in the order of the third, second and first plots until the second sampling. At the third sampling the whole plots showed almost the same growth amount except the first plot which showed a slightly smaller amount. 2) The growth amount per individual plant was in the order of the third, second and first plots until the second sampling, while the first plot showed drastic growth compared with the other two plots at the third sampling. 3) The growth amount by each organ(leaf, stem and root) of the plant body was markedly abundant in the stem of the non-productive part compared with that in the leaf of the productive part. Especially the root was affected remarkably by density and the first plot showed an abundant growth in comparison with the other plots which were too poor to resist the gound surface.

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