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Mass Culture of Ultra-small Rotifer, Synchaeta kitina at the Exchange Rate of Culture Water and Initial Inoculation Density (환수율 및 접종밀도에 따른 초소형 rotifer, Synchaeta kitina의 대량배양)

  • Oh, Jeong-Soo;Park, Jin-Chul;Park, Heum-Gi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.4
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    • pp.354-359
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    • 2009
  • The productivity of ultra-small rotifer, Synchaeta kitina was investigated at the exchange rate of culture water (10, 20, 30, 40 and 50%) and initial inoculation densities (250, 600 and 900 inds. per mL) in semi-continuous culture. Also, the possibility of mass culture was investigated in a 100 L culture tank. Tetraselmis suecica was used as the feed for S. kitina in all experiments. The production of S. kitina increased with an increase in exchange rate of culture water. The highest production ($82.0{\times}10^5$ inds.) was achieved at 40% exchange rate of culture water. The production of S. kitina increased with an increase of initial inoculation density during the first week and the highest total production ($17.4{\times}10^6$ inds.) was achieved at 900 inds. per mL of initial inoculation density. However, on the second week, all treatments were not significantly different in total production (P>0.05). During the two weeks, total production of S. kitina at 900 inds. per mL of initial inoculation density was higher than at 600 inds. of initial inoculation density, but there was no significant difference (P>0.05). In the 100 L culture tank, density of S. kitina was kept from 516 to 890 inds. per mL and S. kitina was daily harvested $15.5{\times}10^6$ to $26.7{\times}10^6$ during the experimental period. The production cost for 100 million rotifers in semi-continuous culture was 63,656 won. The results from this study indicate that the optimal exchange rate of culture water and initial inoculation density for the semi-continuous culture of ultra-small rotifer, S. kitina are 40% and 600 inds. per mL, respectively.

Influence of Tree Thinning on Vegetative Growth, Yield and Fruit Quality of 'Fuji'/M.9 Apple Trees in the High Density Orchard (고밀식 사과원에서의 간벌이 '후지'/M.9 사과나무의 영양생장, 생산량 및 과실품질에 미치는 영향)

  • Hun-Joong Kweon;Dong-Hoon Sagong
    • Korean Journal of Environmental Agriculture
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    • v.42 no.2
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    • pp.104-111
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    • 2023
  • As apple trees mature, it is important to maintain good light distribution within the canopy to produce marketable fruits. Tree thinning is the selective removal of a proportion of trees growing in the orchard to provide more growing space and a good light environment for the remaining trees. This study was conducted over 3 years (14-16 years after planting) to investigate the influence of tree thinning on vegetative growth, yield, fruit quality, and blooming in the 14 years old slender spindle 'Fuji'/M.9 apple trees planted with the tree space of 3.2×1.2 m. The trees were placed in a control group (no thinning; 260 trees per 10 a) or a tree thinning group (thinned 50% of the control; 130 trees per 10 a). The tree thinning successfully improved light penetration, yield per tree, fruit red color, and yield efficiency for 3 years, and the tree thinning controlled the occurrence of biennial bearing. However, tree thinning significantly decreased accumulated yield per 10 a compared with the control. The vegetative growth, yield per tree, soluble solid contents, and blooming were not clear by the occurrence of biennial bearing in the control. These results indicated that tree thinning was a good method for improving light penetration and preventing biennial bearing in the old 'Fuji'/M.9 high-density apple orchards.

Studies on Brewing Conlditions of Takjoo with Commercial Enzyme (Part 2) Quality Examination of Takjoo (시판 정제산소제를 이용한 탁주제조에 관한 연구 (제이보) 탁주의 품질검토)

  • 강효원;권태종;이일근
    • Microbiology and Biotechnology Letters
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    • v.3 no.1
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    • pp.41-48
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    • 1975
  • The chemical components and quality of Takjoo mash were examined on the product brewed by the case of commercial amylase, Lactobacillus bulgaricus and the yeast. 1. Lactic acid was produced at the range of 917~1,350 mg per 100 ml of the mash brewed using wheat flour, corn flour, rice and pressed barley, while succinic acid was formed at the range of 6~32 mg per 100m1 of the mash. 2. Citric acid was formed at the concentration of 6.5mg per 100m1 of the mash on the use of pressed barley, and malic acid formed at the concentration of 1.7 mg and 1.4 mg per 100m1 of the mash respectively, on the use of wheat flour and corn flour. 3. No remarkable differences between the kind of raw materials and commercial amylases and compared with the mash brewed with Nurook were observed in the components of fusel oil formed in the mash. 4. Seventeen kinds of the amino acids of the mash brewed with commercial amylase, Lactobacillus and the yeast were detected and its content was 31mg per 100ml, which is less than that of the conventional takjoo, but the contents of glycine and alanine which pray as the component of seasoning in takjoo were sufficient.

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Growth Characteristics as Influenced by Cutting Site and Planting Method in Autumn Field Cutting of Sedum sarmentosum (돌나물의 가을 노지삽목에서 삽수부위 및 삽식방법에 따른 생육특성)

  • Ahn, Jeong-Ho;Kwon, Ji-Woong;Bae, Jong-Hyang;Lee, Seung-Yeob
    • Journal of Bio-Environment Control
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    • v.17 no.1
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    • pp.60-65
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    • 2008
  • For autumn field cutting of Sedum sarmentosum, the effects of cutting site (distal, middle, and proximal) and planting method (space drill seeding, drill seeding, and broadcast seeding) on survival rate and growth characteristics were investigated at pre- and post-winter season. Plant height root length, stem number per plant, number of branch per plant, fresh weight of shoot, and dry weight of shoot were significantly superior in distal site at pre-wintering (40 days after cutting). At post-wintering (May 10th), stem number per plant, fresh weight and dry weight per $m^2$ in cutting of distal site showed a significant increasement compared to the cutting of proximal site. At pre-wintering (40 days after cutting), the growth in space drill seeding was well than that in drill seeding and broadcast seeding, and fresh weight and dry weight per $m^2$ in space drill seeding were high in order of space drill seeding, broadcast seeding, and drill seeding. At post-wintering (May 10th), stem number per plant, fresh weight and dry weight per $m^2$ in space drill seeding were significantly increased than those in drill seeding. Accordingly, the cutting using distal site of stem in autumn field cutting was desirable for the growth and shoot yield. The space drill seeding showed the highest yield potential among three seeding methods, but broadcast seeding was favorable in saving of labor, because the fresh weight of shoot in broadcast seeding was similar in the space drill seeding at post-wintering.

On the vibration influence to the running power plant facilities when the foundation excavated of the cautious blasting works. (S화력발전소 3, 4호기 증설에 따르는 정밀발파작업으로 인한 인접가동발전기및 구조물에 미치는 파동영향조사)

  • Huh Ginn
    • Explosives and Blasting
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    • v.8 no.1
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    • pp.3-16
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    • 1990
  • The cautious blasting works had been used with emulsion explosion electric M/S delay caps. Drill depth was from 3m to 6m with Crawler Drill $\varphi{70mm}$ on the calcalious sand stone(sort-moderate-semi hard Rock). The total numbers of feet blast were 88. Scale distance were induces 15.52-60.32. It was applied to propagation Law in blasting vibration as follows. Propagtion Law in Blasting Vibration $V=K(\frac{D}{W^b})^n$ where V : Peak partical velocity(cm/sec) D : Distance between explosion and recording sites (m) W : Maximum Charge per delay-period of eighit milliseconds or more(Kg) K : Ground transmission constant, empirically determind on th Rocks, Explosive and drilling pattern ets. b : Charge exponents n : Reduced exponents Where the quantity $D/W^b$ is known as the Scale distance. Above equation is worked by the U.S Bureau of Mines to determine peak particle velocity. The propagation Law can be catagrorized in three graups. Cabic root Scaling charge per delay Square root Scaling of charge per delay Site-specific Scaling of charge per delay Charge and reduction exponents carried out by multiple regressional analysis. It's divided into under loom and over loom distance because the frequency is verified by the distance from blast site. Empirical equation of cautious blasting vibration is as follows. Over 30m----under l00m----- $V=41(D/3\sqrt{W})^{-1.41}$ -----A Over l00m-----$V= 121(D/3\sqrt{W})^{-1.66}$-----B K value on the above equation has to be more specified for furthur understang about the effect of explosives, Rock strength. And Drilling pattern on the vibration levels, it is necessary to carry out more tests.

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On the vibration influence to the running power plant facilities when the foundation excavated of the cautious blasting works (삼천포화력발전소 3, 4호기 증설에 따르는 정밀발파작업으로 인한 인접가동발전기 및 구조물에 미치는 진동영향조사)

  • Huh, Ginn
    • Journal of the Korean Professional Engineers Association
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    • v.24 no.6
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    • pp.97-105
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    • 1991
  • The cautious blasting works had been used with emulsion explosion electric M/S delay caps. Drill depth was from 3m to 6m with Crawler Drill ø70mm on the calcalious sand stone (soft-moderate-semi hard Rock). The total numbers of fire blast were 88 round. Scale distance were induces 15.52-60.32. It was applied to propagation Law in blasting vibration as follows. Propagation Law in Blasting Vibration (Equation omitted) where V : Peak partical velocity(cm/sec) D : Distance between explosion and recording sites(m) W : Maximum Charge per delay-period of eighit milliseconds o. more(kg) K : Ground transmission constant, empirically determind on the Rocks, Explosive and drilling pattern ets. b : Charge exponents n : Reduced exponents Where the quantity D / W$^n$ is known as the Scale distance. Above equation is worked by the U.S Bureau of Mines to determine peak particle velocity. The propagation Law can be catagrorized in three graups. Cubic root Scaling charge per delay Square root Scaling of charge per delay Site-specific Scaling of charge per delay Charge and reduction exponents carried out by multiple regressional analysis. It's divided into under loom and over 100m distance because the frequency is verified by the distance from blast site. Empirical equation of cautious blasting vibration is as follows. Over 30 ‥‥‥under 100m ‥‥‥V=41(D/$^3$√W)$\^$-1.41/ ‥‥‥A Over 100 ‥‥‥‥under 100m ‥‥‥V=121(D/$^3$√W)$\^$-1.56/ ‥‥‥B K value on the above equation has to be more specified for furthur understang about the effect of explosives, Rock strength. And Drilling pattern on the vibration levels, it is necessary to carry out more tests.

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Study on the Salt Tolerance of Rice and Other Crops in Reclaimed Soil Areas 3. Response of Rice to Plant Population and Spacing in the Slty Soil Area (3. 간척지에서 수도개체군과 재식밀도에 관하여)

  • 임형빈
    • Journal of Plant Biology
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    • v.12 no.2
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    • pp.28-42
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    • 1969
  • Field studies were conducted with the split plot design of 20 treatment with a combination of 4 levels of 3, 5, 7 and 9 plants per hill and 5 levels of 60, 80, 100, 120 and 140 hills per 3.3$m^2$ on non-, low- and high-salty areas. Rice variety, Kusabue was grown under jthe standard fertilization and cultivating. Investigation was made on the productive structure of plant population, leaf-area index, light intensity curve by stratum of crop population at the panicle differentiation stage. The competition density effect on the photosynthetic capacity was low as the salt concentration became higher. This seemed to suggest the possiblity of an increased yielding capacity by closer planting in the salty areas. The effect of an increased number of hills per unit area was greater than that of an increased unmber of plants per hill due to the total leaf area and space distribution of the actie assimilation parts of rice plants. The number of ppanicle per unit area in the salty areas were increased when the number of hill per 3.3$m^2$ increased over an increased number of plants per hill, and the panicle weight was reduced by close planting in the non-lalty area, while it was not reduced so much in the salty areas. The number of grains per panicle was significantly decreased by close planting in the salty areas as in the non-salty area, and ratio of matured grain was not decreased even by close planting in the salty areas, while it was significantly decreased by close planting in the non-salty area. An increase in the rice yield was possible by close planting and greatly related to leaf area index in the salty areas but not in the non-salty area. Increasing the number of hills per unit area showed greater effect on the increase of the rice yield than an increased number of plants per hill in the salty areas. Relationships between the growth characteristics and the rice population affected by plant spacing mode for maximum production were discussed.

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Study on the Salt Tolerance of Rice and Other Crops in Reclaimed Soil Areas (Ⅹ) Responce of Rice Population to Varying Plant Density and N Levels in Reclamined Salty Area (간척지에서 수도 및 기타작물의 내염성에 관한 연구 (제10보) 간척지에서 재식밀도와 N 수준 변동에 대한 수도개체군의 반응에 관하여)

  • 임형빈
    • Journal of Plant Biology
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    • v.13 no.3
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    • pp.1-16
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    • 1970
  • Field studies were conducted with kusabue variety and factorial design of 12 treatments composed of 3 levels, 10 kg, 15 kg and 20 kg of N per 10 a , and 4 levels of 80, 100, 120, and 140 hills per $3.3m^2$ plot in reclaimed slaty area having an average of 0.48% salt concentration. The law of spacing effect was observed in the increase of the number of stems at any application levels of N, and the increased N application exceeding 15 kg N per 10 a did not increase the number of stems in maximum tillering stages. The light recieving efficiency of plant population was greatly reduced by close planting when compared with the effect of increased N applications in heading stage. The spacing effect on the C/F ratio was not noted but was reduced markedly by the increased N applications, accordingly the spacing effect on rough rice yields to the LAI was less than by the increase N application. Closer spacing increased the number of panicle, and non-effective stems, decreased the number of grains per panicle and panicle weight. The increased N applications also increased the number of panicle, reduced the weight of 1,000 grains and the ratio of matured grains. It was recommended to plant 100 hills per $3.3m^2$ with the application of 15 kg N per 10 a in the reclaimed salty area of Korea.

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Analysis of Standard Cost and the Construction Period Applied TBM Excavation Method (TBM 굴착 공법 적용 구경별 표준 공사비 및 공사기간 분석 연구)

  • Park, Hong Tae
    • Journal of the Society of Disaster Information
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    • v.8 no.4
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    • pp.319-327
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    • 2012
  • This research collected work drilling report of TBM method site developed by WRITH company to TBM equipment company in Germany and analyzed work operating productivity. Based the data analyzed TBM operating productivity, This research derived and presented excavation speed(m/day) by TBM diameter (2.6m, 3.0m, 3.5m, 5.0m, 8.0m) and rock. Also, based on the excavation speed(m/day) by TBM diameter, This research estimated a day direct construction cost and total direct construction costs by applying a direct construction cost which spent on per 1m. Based on hard rock 40.0%, soft rock 30.0%, usually rock 20%, weathered rock 10.0%, TBM boring velocity, m per cost, total construction period shall estimate the construction cost and the construction period by the effective use by diameter when future applying TBM method to the basic planning stages and in the preliminary design phase.

Fetal Sex Determination by RIA of Amniotic Fluid Testosterone and FSH (태아성별(胎兒性別)에 따른 양수중(羊水中) Testosterone과 F.S.H.의 동태(動態)에 관(關)한 연구(硏究))

  • Koh, Min-Whan;Shin, Myon-Woo
    • Clinical and Experimental Reproductive Medicine
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    • v.6 no.1_2
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    • pp.23-28
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    • 1979
  • To determine whether hormone analysis of amniotic fluid could be used for accurate determination of fetal sex, we measured testosterone(T) and follicle-stimulating hormone in 19 amniotic fluid samples. The mean T in amniotic fluid of 8 women earring male fetuses was 310 pg. per milliliter and of 11 women earring female fetuses was 150 pg. per milliliter (P<0.05${\ast}$). The mean amniotic fluid FSH of 1.16 mI.U. per milliliter for 7 women with male fetuses was over trifold lower than that for subjects with female fetuses. The mean amniotic fluid FSH of female fetuses was 3.85 mI.U. per milliliter (P<0.01${\ast}$) Measurement of T & FSH in amniotic fluid may be an adjunct method for fetal sex determination.

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