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Mass Culture of Ultra-small Rotifer, Synchaeta kitina at the Exchange Rate of Culture Water and Initial Inoculation Density

환수율 및 접종밀도에 따른 초소형 rotifer, Synchaeta kitina의 대량배양

  • Oh, Jeong-Soo (Faculty of Marine Bioscience & Technology, Gangnung-Wonju National University) ;
  • Park, Jin-Chul (Faculty of Marine Bioscience & Technology, Gangnung-Wonju National University) ;
  • Park, Heum-Gi (Faculty of Marine Bioscience & Technology, Gangnung-Wonju National University)
  • 오정수 (강릉원주대학교 해양생명공학부) ;
  • 박진철 (강릉원주대학교 해양생명공학부) ;
  • 박흠기 (강릉원주대학교 해양생명공학부)
  • Published : 2009.08.31

Abstract

The productivity of ultra-small rotifer, Synchaeta kitina was investigated at the exchange rate of culture water (10, 20, 30, 40 and 50%) and initial inoculation densities (250, 600 and 900 inds. per mL) in semi-continuous culture. Also, the possibility of mass culture was investigated in a 100 L culture tank. Tetraselmis suecica was used as the feed for S. kitina in all experiments. The production of S. kitina increased with an increase in exchange rate of culture water. The highest production ($82.0{\times}10^5$ inds.) was achieved at 40% exchange rate of culture water. The production of S. kitina increased with an increase of initial inoculation density during the first week and the highest total production ($17.4{\times}10^6$ inds.) was achieved at 900 inds. per mL of initial inoculation density. However, on the second week, all treatments were not significantly different in total production (P>0.05). During the two weeks, total production of S. kitina at 900 inds. per mL of initial inoculation density was higher than at 600 inds. of initial inoculation density, but there was no significant difference (P>0.05). In the 100 L culture tank, density of S. kitina was kept from 516 to 890 inds. per mL and S. kitina was daily harvested $15.5{\times}10^6$ to $26.7{\times}10^6$ during the experimental period. The production cost for 100 million rotifers in semi-continuous culture was 63,656 won. The results from this study indicate that the optimal exchange rate of culture water and initial inoculation density for the semi-continuous culture of ultra-small rotifer, S. kitina are 40% and 600 inds. per mL, respectively.

Keywords

References

  1. Assavaaree, M., A. Hagiwara and K Lubzens, 2001a. Factors affecting low temperature preservation of the marine rotifer Brachionus rotundiformis Tschugunoff. Hydrobiologia, 446/447, 355-361 https://doi.org/10.1023/A:1017515209173
  2. Assavaaree, M., A. Hagiwara, K, Ide, K, Maruyama and E. Lubzens. 2001 b, Low-temperature preservation (at $4{^{\circ}C}$) of marine rotifer Brachionus. Aquacult Res., 32, 29-39 https://doi.org/10.1046/j.1365-2109.2001.00524.x
  3. Boraas, M.K 1983, Population dynamics of food-limited rotifers in two-stage chemostat culture, Limnol. Oceanogr., 28, 546-563 https://doi.org/10.4319/lo.1983.28.3.0546
  4. Dhert, P., G. Rombaut, G, Suantika and P. Sorgeloos. 2001. Advancement of rotifer culture and manipulation techniques in Europe, Aquaculture, 200, 129-146 https://doi.org/10.1016/S0044-8486(01)00697-4
  5. Duncan, D.B. 1955. Multiple-range and multiple F tests, Biometrics, 11, 1-42 https://doi.org/10.2307/3001478
  6. Duray, M,N., C.B. Estudillo and L.G, Alpasan, 1997, Larval rearing of the grouper Epinephelus suillus under laboratory conditions. Aquaculture, 150, 63-76 https://doi.org/10.1016/S0044-8486(96)01467-6
  7. Kohno, H., R,S, Ordonio-Aguilar, A. Ohno and Y. Taki. 1997. Why is grouper larval rearing difficult?: an approach from the development of the feeding apparatus in early stage larvae of the grouper, Epinephelus coioides. Ichthyol. Res., 44, 267-274 https://doi.org/10.1007/BF02678706
  8. Lee, K.W., H.G. Park and S.H. Cho. 2001. Productivity of freshwater rotifer, Brachionus calyciflorus and marine rotifer, B. rotundiformis in the semi-continuous high density culture. J. Kor. Fish. Soc., 34, 156-159
  9. Lubzens, E., O. Zmora and Y. Barr. 2001. Biotechnology and aquaculture of rotifers. Hydrobiologia, 446/447, 337-353 https://doi.org/10.1023/A:1017563125103
  10. Ooms-Wilms, A.L., G. Postema and R.D. Gulati. 1999. Population dynamics of planktonic rotifers in Lake Loosdrecht (The Netherlands) in relation to their potential food and predators. Freshwater Biology, 42, 77-97 https://doi.org/10.1046/j.1365-2427.1999.00463.x
  11. Park, H.G., K.W. Lee and S.K. Kim. 1999. Growth of rotifer by the air, oxygen gas-supplied and the pH-adjusted and productivity of the high density culture. J. Kor. Fish. Soc., 32, 753-757
  12. Park, H.G., K.W. Lee, S.H. Cho, H.S. Kim, M.M. Jung and B.S. Kim. 2001. High density culture of the freshwater rotifer, Brachionus calyciflorus. Hydrobiologia, 446/447, 369-374 https://doi.org/10.1023/A:1017571327829
  13. Park, J.C. 2007. Mass culture of rotifer, Synchaeta sp .. M.S. Thesis, Kangnung-Wonju National University, Gangneung, Korea, pp. 50
  14. Park, J.C. and H.G. Park. 2008a. Optimal salinity and temperature conditions for the growth of the ultrasmall roti fer Synchaeta kitina. J. Aquaculture, 21, 70-75
  15. Park, J.C. and H.G. Park. 2008b. Optimal food and concentration for the growth of the ultra-small rotifer Synchaeta kitina. J. Aquaculture, 21, 76-81
  16. Schluter, M. and 1. Groeneweg. 1985. The inhibition by ammonia of population growth of the rotifer, Brachionus rubens, in continuous culture. Aquaculture, 46, 215-220 https://doi.org/10.1016/0044-8486(85)90207-8
  17. Shiel, R.J. and W. Koste. 1993. Rotifera from australian inland waters. IX. Gastropodidae, Synchaetidae, Asplanchnidae (Rotifera: Monogononta). Trans. Roy. Soc. S. Aust., ll7, lll-l39 https://doi.org/10.1007/s10750-005-4092-7
  18. SPSS Inc .. 2005. SPSS Base 14.0 for windows, SPSS Inc., 444N. Michigan Avenue, Chicago, IL, USA
  19. Suchar, V.A. and P. Chigbu. 2006. The effects of algae species and densities on the population growth of the marine rotifer, Colurella dicentra. J. Exp. Mar. BioI. Ecol., 337, 96-102 https://doi.org/10.1016/j.jembe.2006.06.015
  20. Yoshimura, K., E. Kitajima, Y. Miyamoto and G. Kishimoto. 1994. Factors inhibiting growth of the rotifer Brachionus plicatilis in high density cultivation by feeding condensed Chlorella. Nippon Suisan Gakkaishi, 60, 207-213 https://doi.org/10.1016/S0044-8486(03)00501-5
  21. Yoshimura, K., K. Usuki, T. Yoshimatsu, e. Kitajima and A. Hagiwara. 1997. Recent development of a high density mass culture system for the rotifer Brachionus rotundiformis Tschugunoff. Hydrobiologia, 358, 139-144 https://doi.org/10.1023/A:1003169414996
  22. Yoshimura, K., K. Tanaka and T. Yoshimatsu. 2003. A novel culture system for the ultra-high-density production of the rotifer, Brachionus rotundiformis-a preliminary report. Aquaculture, 227, 165-172 https://doi.org/10.1016/S0044-8486(03)00501-5
  23. Yu, J. and K. Hirayama. 1986. The effect of un-ionized ammonia on the population growth of the rotifer in mass culture. Bull. Jap. Soc. Sci. Fish., 52, 1509-15l3 https://doi.org/10.1016/S0044-8486(99)00271-9