• International Journal of Fuzzy Logic and Intelligent Systems
• /
• v.9 no.2
• /
• pp.115-127
• /
• 2009

In the present paper we introduce and study L-pre-$T_0$-, L-pre-$T_1$-, L-pre-$T_2$ (L-pre-Hausdorff)-, L-pre-$T_3$ (L-pre-regularity)-, L-pre-$T_4$ (L-pre-normality)-, L-pre-strong-$T_3$-, L-pre-strong-$T_4$-, L-pre-$R_0$-, L-pre-$R_1$-separation axioms in (2, L)-topologies where L is a complete residuated lattice.Sometimes we need more conditions on L such as the completely distributive law or that the "$\bigwedge$" is distributive over arbitrary joins or the double negation law as we illustrate through this paper. As applications of our work the corresponding results(see[1,2]) are generalized and new consequences are obtained.

• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.12
• /
• pp.1749-1754
• /
• 2003

The study was undertaken to see the effect of elevated feeding during pre-partum or pre- as well as post-partum period on the productive and reproductive performance of crossbred cows. The experiment lasted for 60 d pre-partum to 120 d post-partum. Eighteen dry pregnant crossbred cows divided into three equal groups were fed either as per NRC feeding standard (C) or 20% above NRC during 60 d pre-partum ($T_1$) or fed 20% above NRC during both 60 d pre-partum to 120 d post-partum ($T_2$) period. During prepartum period body weight gain was significantly ($p{\leq}0.05$) higher in $T_1$ and $T_2$ groups than that of control group. The animals fed at higher plane of nutrition ($T_1$ and $T_2$) took significantly lesser time for complete relaxation of pelvic muscles, act of calving and for expulsion of placenta than that of control group. Moreover, such cows delivered 2 to 3 kg heavier calves as compared to normal fed dams. During post-partum period, the average daily milk yield was significantly higher in $T_2$ group than that in $T_1$ and control groups. The peak yield was significantly higher in $T_2$ group, it took longer time to reach peak production but it was more persistent in this group as compared to $T_1$ and control groups. Average milk fat, solids-not-fat (SNF) and total solids were significantly higher in $T_1$ and $T_2$ groups as compared to control group. Body weight losses incurred during early lactation were not even compensated by end of 4th month of lactation in C and $T_1$ groups whereas the animals in $T_2$ group gained 2.0 kg. The 1st post-partum estrus and conception rate were better in high fed groups ($T_1$ and $T_2$) than that of control group. The returns over feed cost of milk production were higher in $T_2$ group followed by $T_1$ and control groups indicating the advantage of elevated feeding during pre- and post-partum periods.

• Animal cells and systems
• /
• v.1 no.1
• /
• pp.87-91
• /
• 1997

Effects of divalent cations such as $Mg^{2+}$, $Mn^{2+}$, $Ca^{2+}$, and $Zn^2$ on splicing activity of phage T4 thymidylate synthase intron RNA have been investigated. At the concentration of 0.5 mM, $Mn^{2+}$ in the absence of $Mg^{2+}$, a very small amount of pre-RNA was cleaved into ligation products (El-E2) but no circular or linear intron was produced. As the concentration of $Mn^{2+}$ was increased from 1 to 5 mM the pre-RNA was completely hydrolyzed. In the presence of 5 mM $Mg^{2+}$, both the linear intron and circular intron were produced but no El-E2 ligation product was produced. At both 3 and 5 mM $Mn^{2+}$ the RNA was hydrolyzed completely as observed with no $Mg^2+$ being present. In the case of $Zn^{2+}$, even at 0.5 mM concentration, the pre-RNA was completely hydrolyzed. This observation suggested that $Zn^{2+}$ facilitates RNA hydrolysis more rapidly than $Mn^{2+}$ does. at 5mM $Ca^{2+}$, the RNA was not hydrolyzed and remained intact as a primary transcript.

• Bulletin of the Korean Chemical Society
• /
• v.26 no.12
• /
• pp.2033-2037
• /
• 2005

In vitro selection was used to isolate $Mg^{2+}$-dependent self-cleaving ribozymes with cis-cleavage activity from a pre-tRNA library having 40-mer random sequences attached to 5'-end of E. coli $tRNA^{Phe}$. After 8 rounds of SELEX (Systematic Evolution of Ligands by Exponential Enrichment), RNA molecules which can self-cleave at the high concentration of $Mg^{2+}$ were isolated. The selected ribozymes can carry out the self-cleavage reaction in the presence of 100 mM $Mg^{2+}$ but not in 10 mM $Mg^{2+}$. The cleavage sites of the ribozymes are located at +3 and +4 of $tRNA^{Phe}$, compared with +1 position of 5'-end cleavage site of pre-tRNA by RNase P. New RNA constructs deprived of its D stem-loop, anticodon stem-loop, variable loop and T stem-loop, respectively showed the cleavage specificity identical to a ribozyme having the intact tRNA structure. Also, the new ribozyme fused with both a ribozyme and $tRNA^{Leu}$ showed the cleavage activities at the various sites within its sequences, different from two sites of position +3 and +4 observed in the ribozyme with $tRNA^{Phe}$. Our results suggest that the selected ribozyme is not structural-specific for tRNA.

• Kyungpook Mathematical Journal
• /
• v.54 no.2
• /
• pp.173-188
• /
• 2014

In this paper, a new class of open sets, namely ${\gamma}^*$-pre-open sets was introduced and its basic properties were studied. Moreover a new type of topology ${\tau}_{{\gamma}p^*}$ was generated using ${\gamma}^*$-pre-open sets and characterized the resultant topological space (X, ${\tau}_{{\gamma}p^*}$) as ${\gamma}^*$-pre-$T_{\frac{1}{2}}$ space.

• Bulletin of the Korean Chemical Society
• /
• v.27 no.10
• /
• pp.1618-1622
• /
• 2006

Bacteriophage T7 gp4A' is a ring-shaped hexameric DNA helicase that catalyzes duplex DNA unwinding using dTTP hydrolysis as an energy source. To investigate the effect of single-stranded DNA (ssDNA) on the kinetic pathway of dTTP hydrolysis by the T7 DNA helicase complexed with ssDNA, we have first determined optimal concentration of long circular M13 single-stranded DNA and pre-incubation time in the absence of $Mg^{2+}$ which is necessary for the helicase-ssDNA complex formation. Steady state dTTP hydrolysis in the absence of $Mg^{2+}$ by the helicase-ssDNA complex provided $k_{cat}$ of $8.5\;{\times}\;10^{-3}\;sec^{-1}$. Pre-steady state kinetics of the dTTP hydrolysis by the pre-assembled hexameric helicase was monitored by using the rapid chemical quench-flow technique both in the presence and absence of M13 ssDNA. Pre-steady state dTTP hydrolysis showed distinct burst kinetics in both cases, indicating that product release step is slower than dTTP hydrolysis step. Pre-steady state burst rates were similar both in the presence and absence of ssDNA, while steady state dTTP hydrolysis rate in the presence of ssDNA was much faster than in the absence of ssDNA. These results suggest that single-stranded DNA stimulates dTTP hydrolysis reaction by T7 helicase by enhancing the rate of product release step.

• Journal of the Chungcheong Mathematical Society
• /
• v.24 no.1
• /
• pp.11-17
• /
• 2011

We introduce the notion of weak M-precontinuity which is a generalization of M-precontinuity defined between spaces with minimal structures. We also investigate some properties and characterizations for the continuity.

• Clinical and Experimental Reproductive Medicine
• /
• v.28 no.2
• /
• pp.79-86
• /
• 2001

Objective: Mouse pre-antral follicles require the addition of gonadotropins (Gns) to complete maturation and ovulation of oocyte and antrum formation in vitro. However, we tried examination of in vitro growth of mouse pre-antral follicles in medium without Gns and/or phygiological factors. And also, pre-antral follicles were isolated from ovaries by mechanical method. Our present studies were conducted to evaluate on the growth of follicles and intra-follicular oocytes and antrum formation in vitro of mouse pre-antral follicles in two different media. Methods: Pre-antral follicles ($91{\sim}120{\mu}m$) were isolated mechanically by fine 30G needles not using enzymes from ovaries of 3-6 week-old female ICR mice. Isolated pre-antral follicles were cultured in $20{\mu}l$ droplets of TCM (n=17; follicles: $107.8{\pm}1.58{\mu}m$; oocytes: $57.9{\pm}1.2{\mu}m$) or MEM (n=12; follicles: $109.3{\pm}2.53{\mu}m$; oocytes: $55.4{\pm}1.6{\mu}m$) under mineral oil on the 60 mm culture dish. All experimental media was supplemented with 10% FBS without Gns and/or physiological factors. Pre antral follicles were individually cultured for 8 days. Antram formation and growth of pre-antral follicles and intra-follicular oocytes were evaluated using precalibrated ocular micrometer at X200 magnifications during in vitro culture. Results were analyzed using combination of Student's t-test and Chi-square, and considered statistically significant when p<0.05. Results: Antrum formation had started in two culture media on day 2. On day 8, antrum formation had occurred in 58.3% of pre-antral follicles cultured in DMEM, but only in 23.5% of those cultured in TCM (p=0.0364). Growth of pre-antral follicles and intra-follicular oocytes were observed on day 4 and 8. On day 4, follicular diameter was similar (p=0.1338) in TCM ($119.4{\pm}2.58{\mu}m$) and MEM ($125.4{\pm}4.52{\mu}m$). However, on day 8, diameters of pre-antral follicles cultured in MEM ($168.9{\pm}17.29{\mu}m$) were significantly bigger (p=0.0248) than that in TCM ($126.7{\pm}4.28{\mu}m$). On day 4 and 8, diameters of intra-follicular oocytes were similar in TCM ($67.1{\pm}1.3$ and $72.4{\pm}0.9{\mu}m$) and MEM ($65.2{\pm}1.7$ and $73.3{\pm}1.5{\mu}m$), respectively. Conclusion: We can conform that medium without Gns and/or physiological factors can be used for in vitro antrum formation and growth of pre-antral follicles and intra-follicular oocytes in mouse. In conclusion, MEM supplemented with FBS can be used for growth in vitro of mouse pre-antral follicles isolated mechanically.

• Asian-Australasian Journal of Animal Sciences
• /
• v.5 no.4
• /
• pp.659-664
• /
• 1992

Twelve day old Pabna zebu calves of similar weights were assigned at random to 3 different calf starter-rations namely $T_0$, $T_1$ and $T_2$ to determine the effect of feeding regimes and growth pattern of calves upto 3 months of age. Calves allowed to intake starter 0.5 percent of body weight from 2nd week on to 7th weeks of age, while starter at 1 percent of body weight was allowed later on upto 13th weeks of age. Calves started to intake green grass after 2nd to 3rd weeks and average intake per day was limited to 1 kg upto the end of experiment, while calves started to take rice straw ad libitum after 6th weeks of age. Colostrum feeding was offered ad libitum and in addition calves suckled their dam's milk shortly before and after milking usually in the morning and evening. Growth of calves in different treatment groups was found statistically insignificant. The mean growth rate per head per day ranged from 196.43-375.0 g for $T_0$ group, 185.72-360.72 g for $T_1$ group and 180.1-385.72 g for $T_2$ group respectively between 1st to 7th weeks of age while the growth rate ranged from 309.53-328.57 g for $T_0$, 304.29-342.86 g for $T_1$ and 304.77-333.30 g for $T_2$ groups respectively from 9th weeks on to the end of the experiment.

• BMB Reports
• /
• v.40 no.6
• /
• pp.1002-1008
• /
• 2007

PreS domain of Hepatitis B virus (HBV) surface antigen is a good candidate for an effective vaccine as it activates both B and T cells besides binding to hepatocytes. This report deals with overexpression and purification of adr subtype of surface antigen that is more prevalent in Pakistan. PreS region, comprising 119 aa preS1 region plus a 55 aa preS2 region plus 11 aa from the N-terminal S region, was inserted in pET21a+ vector, cloned in E. coli $DH5\alpha$ cells and expressed in E. coli BL21 codon+ cells. The conditions for over expression were optimized using different concentrations of IPTG (0.01-5 mM), and incubating the cells at different temperatures (23-$41^{\circ}C$) for different durations (0-6 h). The cells were grown under the given optimized conditions (0.5 mM IPTG concentration at $37^{\circ}C$ for 4 h), lysed by sonication and the protein was purified by ion exchange chromatography. On the average, 24.5 mg of recombinant protein was purified per liter of culture. The purified protein was later lyophilized and stored at $-80^{\circ}C$.