• KSBB Journal
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• 제19권1호
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• pp.42-49
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• 2004

Racemic epoxide에 대한 입체선택적 가수분해능을 가지고 있는 곰팡이, Aspergillus niger LK로부터 epoxide hydrolase (EH, EC 3.3.2.3) 유전자의 진화적 유연관계 분석을 행하였다. A. niger LK의 EH 염기서열로부터 유추한 EH 단백질 아미노산 서열은 여러 박테리아의 EH들 및 포유동물의 microsomal EH들과 유의적인 유사성을 가지고 있었으며 a/$\beta$ hydrolase fold family에 속하였다. A. niger LK의 EH 단백질의 입체구조예측은 Protein Data Bank에 수록된 lqo7의 3D 결정구조와 90.6% identity를 가지는 것으로 나타났으며 다른 EH들의 아미노산 서열비교를 행한 결과 Asp$^{192}$ , Asp$^{348}$ 및 His$^{374}$ 이 catalytic triad를 구성하고 있는 것으로 추정되었다. 여러 생물종의 EH서열을 기능적 및 구조적 domain 서열을 기초로 하여 multiple sequence alignment를 행하고 Neighbor-Joining/UPGMA method를 이용하여 계통수를 복원한 결과 다른 생물종들의 EH와의 진화거리는 서로 1.841∼2.682로 멀었으나 EH의 기능을 가지기 위한 oxyanion hole 및 a/$\beta$ hydrolase fold family의 catalytic triad는 잘 보존되고 있어 공통조상으로부터 진화되어 왔음을 알 수 있었다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권7호
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• pp.3131-3138
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• 2016

Several studies have addressed the possible role of hepatitis C virus genotype-4 (HCV GT4) in apoptosis. However, this still not fully understood. In the current study a re-constructed clone of E1/E2 polyprotein region of the HCV GT4 was transfected into the Huh7 cell line and a human apoptotic PCR array of 84 genes was used to investigate its possible significance for apoptosis. Out of the 84 genes, only 35 showed significant differential expression, 12 genes being up-regulated and 23 down-regulated. The highest-up regulated genes were APAF1 (apoptotic peptidase-activating factor 1), BID (BH3 interacting domain death agonist) and BCL 10 (B-cell CLL/lymphoma protein 10) with fold regulation of 33.2, 30.1 and 18.9, respectively. The most down-regulated were FAS (TNF receptor super family), TNFRSF10B (tumor necrosis factor receptor super-family member 10b) and FADD (FAS-associated death domain) with fold regulation of -30.2, -27.7 and -14.9, respectively. These results suggest that the E1/E2 proteins may be involved in HCV-induced pathogenesis by modulating apoptosis through the induction of the intrinsic apoptosis pathway and disruption of the BCL2 gene family.

• 대한간호학회지
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• 제29권3호
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• pp.639-655
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• 1999

An explanatory design was employed to identify the relationship of physical, emotional & social readjustment and social support of post hospitalized stroke patients and their caregivers. A convenient sample of 254 patients who given follow-up care at the outpatient department after discharge and 225 caregivers were recruited. Mental Status Questionnaire (MSQ), Social Support Inventory Stroke Survivors (SSISS), Illness intrusiveness(II), Instument Activity of Daily Living(IADL), Center of Epidemilogic Studies-Depression(CES-D), social activity and caregiver burden were used for measurement in this study. Results showed patient's physical level measured by IADL and psychological level measured by depression were high. But social activity was low. Cognitive function, depression & social activity were not significantly different by the posthospitalized period, but IADL was. The source of professional support was mostly the physician at the outpatient department. The family support was found significantly related to patient's depression & social activity and caregiver's subjective burden. Professional support was found significantly related to patient's IADL & depression. Illness intrusiveness as a mediating variable was a sig nificantly predicting power on patient's IADL & depression. The path analysis was used to identify the variables to predict the physical, emotional, and social status of patients. As a result, patient's age, cognitive function, illness intursiveness and professional support significantly predicted the level of IADL ; patient's cognitive function, illness intrusiveness and family support significantly predicted the level of depression ; and patient's age and family support significantly predicted the level of social activity of posthospitalized stroke patients. Based upon these results, the rehabilitation programs to reduce the illness intrusiveness and improve cognitive funtion were recommended for the readjustment of the stroke patients. This model of the readjustment of the posthospitalized stroke patients is recommended as the framework for care of the stroke patients.

• Animal cells and systems
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• 제13권4호
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• pp.391-397
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• 2009

The house dust mite (Dermatophagoides pteronissinus) is an important factor in triggering allergic diseases. The function of eosinophils, particularly in the production of cytokine or chemokine, is critical in understanding the pathogenesis of inflammatory diseases. In this study, we examined whether D. pteronissinus extract (DpE) induces the expression of monocyte chemotactic protein 1 (MCP-1)/CCL2, IL-8/CXCL8, and IL-6 that mediate in the infiltration and activation of immune cells and in its signaling mechanism in the human eosinophilic cell line, EoL-1. DpE increased the mRNA and protein expression of MCP-1, IL-8, and IL-6 in a time- and dose-dependent course in EoL-1 cells. In our experiments using signal-specific inhibitors, we found that the increased expression of MCP-1, IL-8, and IL-6 due to DpE is associated with Src family tyrosine kinase and protein kinase C $\delta$ (PKC $\delta$). In addition, the activation of extracellular signal-regulated kinase (ERK) is required for MCP-1 and IL-8 expression while p38 mitogen-activated protein kinase (MAPK) is involved in IL-6 expression. DpE induced the phosphorylation of ERK and p38 MAPK. PP2, an inhibitor of Src family tyrosine kinase, and rottlerin, an inhibitor of PKC $\delta$, blocked the activation of ERK and p38 MAPK. DpE induces the activation of ERK and p38 MAPK via Src family tyrosine kinase and PKC $\delta$ for MCP-1, IL-8, or IL-6 production. Increased cytokine release due to the house dust mite and the characterization of its signal transduction may be valuable in understanding the eosinophil-related pathogenic mechanism of inflammatory diseases.

• 한국인구학
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• 제7권1호
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• pp.39-77
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• 1984

It has been well known that the family pjanning programme has a great deal with declining fertility in Korea. The present study was aimed to measure the impact of family planning program me on fertility level. The specific objectives are to overview the demographic changes ncluding fertility declining and to calculate actual number of birth averted during the last 20 years. The data used for the present study was collected from various sources such as service statistics of the government, survey and research reports, and other related papers. The tool used for calculation of birth prevention was "Standard Couple Years of Protection (SCYP) Method" developed by M Gorosh. The major findings are summarized as follows ; Total of family planning acceptors from 1962 to 1982 was 9,556,153. On the basis of this data, total of averted births was calculated for eachcontraceptive Method ; 1.Oral pills, total number of acceptors from 1968 to 1982 was 2.347,259. Estimated number of birth averted was 272 ,303 .70 and the Coefficient of births prevention per acceptor turned out to be 0.114 2.Condoms, total number of birth acceptors from 1962 to 1982 was 2,874,216. Estimated number of birth averted was 220,495.30 and the coefficient of births prevention per acceptor turned out to be 0.077. 3.IUD, total number of acceptors from 1962 to 1982 was 5,101 .650. Estimated number of birth averted was 1,377,584.76 and the coefficient of births prevention per acceptor turned out to be 0.270. 4.Vasectomy, total number of acceptors from 1962 to 1982 was 556,508. Estimated number of birth averted was 626 ,128 .80 and the coefficient of births prevention per acceptor turned out to be 1.125. 5.Tubaligation total number of birth acceptors from 1972 to 1982 was 1 210,775. Estimated number of birth averted was 1 ,117 679.00 and thecoefficient of birtlts prevention per acceptor turned nut to be 0.923. 6.Abortion, total number of acceptors from 1973 to 1982 was 465,525. Estimated number of birth averted was 355 ,283 .60 and the coefficient of births prevention per acceptor turned out to be 0.763.

• Journal of Korean Biological Nursing Science
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• 제19권2호
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• pp.113-122
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• 2017

Purpose: The purpose of this study was to identify influencing factors on rehabilitation adherence in stroke patients. Methods: This study was a descriptive survey. A structured questionnaire was used for face-to-face interviews with a convenient sample of 192 subjects, who were admitted in 5 rehabilitation hospitals located in G metropolitan city. Results: The score of rehabilitation motivation in the subjects was a mean of $2.04{\pm}0.35$, self-efficacy $6.22{\pm}2.32$, family support $3.40{\pm}0.82$ and rehabilitation adherence $3.08{\pm}0.41$. The rehabilitation adherence was a statistically significant difference according to the education level (F= 3.40, p= .035), marital status (F= 4.04, p= .019), number of personal insurance policies (K= 9.80, p= .020), location of paresis (F= 2.72, p= .046), and status of current smoking (M = 657.00, p= .001). There was significant correlation among degree of rehabilitation adherence, rehabilitation motivation (r= .30, p< .001), self-efficacy (r= .14, p= .046) and family support (r= .18, p= .011). Rehabilitation motivation (${\beta}=0.19$, p= .007), self-efficacy (${\beta}=0.14$, p= .035), marital status (${\beta}=0.14$, p= .038), number of personal insurance policies (${\beta}=-0.15$, p= .045) and location of paresis(${\beta}=-0.15$, p= .028) were identified as significant predictors. This model explained 22.6% of variance in rehabilitation adherence (F= 5.92, p< .001). Conclusion: There is a need to develop an effective intervention for rehabilitation adherence improvement considering the identified variables in this study.

• Child Health Nursing Research
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• 제6권3호
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• pp.362-371
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• 2000

The purpose of this study was to examine the stressors, coping styles, communication with mother, school adaptation and predictors for school adaptation. The subjects of this study consisted of 354 children. They were 6th grade of elementary school in Taegu city. The periods of study was from march 10, to march 18, 2000. Data were analyzed by SAS computer programme. The results of this study were as follows: 1. The mean score for stressor was 39.91 in children(range 24-72). The higher stressor among three subgroups of stressor was mass media stressor. The degree of stressor revealed to be influenced significantly economic status. 2. The most frequently used coping style was problem-oriented coping style(M=18.94) in children. The degree of emotion-oriented coping style revealed to be influenced significantly family's atmosphere 3. The mean score for communication with mother was 72,44 in children(range 20- 100). The degree of communication with mother revealed to be influenced significantly sex, economic status, family' atmosphere. 4. The mean score for school adaptation was 62.48 in children(range 25-100). The higher school adaptation among five subgroup was rule dimension. The degree of school adaptation revealed to be influenced significantly sex, birth, mother's job, economic status, family's atmosphere. 5. School adaptation were positively correlated with communication with mother, problem- oriented coping style(r=0.40 p<0.001) (r=0.30 p<0.001). 6. School adaptation were negatively correlated with emotion-oriented coping style, stressor (r=-0.16 p<0.01)(r=-0.13 p<0.01). 7. Forward multiple regression analysis revealed that the most powerful predictor was the variable communication with mother accounted for 15.25% in school adaptation. All variables accounted for 25.55% in school adaptation. From the above results, it can be concluded that teaching mother about importance of communication, encouraging to use problem-oriented coping style is necessary to improve school adaptation in 6th grade elementary school children

• 한국안광학회지
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• 제13권3호
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• pp.89-94
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• 2008

목적: 본 논문은 소아 및 학동기 그리고 청소년과 부모시력의 유전적 연관성을 보고자 하였다. 방법: 연구 대상자는 4~18세까지의 소아 및 학동기 그리고 청소년기에 속하는 어린이 208명과 피검자의 부모 340명이 참여하였다. 시력측정은 5 m용 한천석식 시력표를 이용하였으며, 각 단안씩 나안 및 교정시력을 측정하였다. 결과: 6세미만의 소아에서 아버지의 시력은 아무런 관련성을 찾을 수 없었으나, 남아에서는 어머니의 시력에 영향을 받는 것으로 나타났다. 학동기의 경우는 특히 부모와의 관련성이 높은 것으로 나타났는데 시력이 0.7이하인 그룹에서는 부모 모두 0.7이하가 많았으며, 0.7초과인 그룹에서는 부모도 같이 0.7을 초과하는 경향을 보였다. 청소년기의 경우에서도 어머니의 시력이 남아의 시력과 연관성이 있음을 보여주었다. 결론: 부모의 가족력이나 특히 어머니의 시력이상은 자녀의 시력이상에 더욱더 영향을 미치는 것으로 나타났다.

• Safety and Health at Work
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• 제12권2호
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• pp.167-173
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• 2021

Background: Correctional officers (COs) experience elevated rates of mental and physical ill-health as compared with other general industry and public safety occupations. The purpose of this study was to investigate demographic, mental health, job tenure, and work-family characteristics and their prospective association to burnout within and between jail officers during one year of new employment. Methods: In 2016, newly hired jail officers (N = 144) completed self-reported surveys across four time points in a one-year prospective study at a Midwestern United States urban jail. Linear mixed-effects and growth modeling examined how work-family conflict (W-FC) and depressive symptoms relate to perceptions of burnout over time. Results: Jail officer burnout increased and was related to rises in W-FC and depression symptoms. Within-person variance for W-FC (B_pooled = .52, p < .001) and depression symptoms (B_pooled = .06, p < .01) were significant predictors of burnout. Less time on the job remained a significant predictor of burnout across all analyses (B_pooled = .03, p < .001). Conclusions: Results from this study indicate that burnout increased during the first year of new employment; and increased W-FC, higher depression, and brief tenure were associated with burnout among jail COs. Future study of correctional workplace health is needed to identify tailored, multilevel interventions that address burnout and W-FC prevention and early intervention among COs.

• 한국환경보건학회지
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• 제41권2호
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• pp.61-70
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• 2015

Objectives: The purpose of this research was to examine radon exposure in terms of the relationship between the living environment and indoor radon concentrations among vulnerable households. Methods: Nationwide, 1,129 subjects were selected using personal questionnaires for adequately understanding the living environment, installation of E-PERM radon gas detectors, and investigation of the structure of the housing. Results: The mean concentration of indoor radon for all subjects was $130.2Bq/m^3$ (GM=101.7), and a total of 438 subjects (38.8%) exceeded the recommended standards ($148Bq/m^3$) for public facilities by the Ministry of the Environment. By location, the highest concentrations ($164.3Bq/m^3$, GM=124.1) were seen in North Chungcheong Province. In the case of the Seoul/Gyeonggi Province metropolitan area, they showed $125.6Bq/m^3$ (GM=105.1) and $118.9Bq/m^3$ (GM=96.5), respectively. By type of housing, indoor radon concentrations in single-family housing were higher than in row/multi-family housing (p<0.01). Although indoor radon concentrations raised in accordance with year of construction (p<0.05), the difference between indoor radon concentrations in underground residences was not observed to be statistically significant (p=0.633). Conclusion: More studies are necessary in the future regarding the difference in indoor radon concentrations that may occur due to different of types of indoor construction, building materials, and the amount of building materials.