• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.9C
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• pp.669-675
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• 2008

For an odd prime p, n=4k and $d=((p^2k+1)/2)^2$, there are $(p^{2k}+1)/2$ distinct decimated sequences, s(dt+1), $0{\leq}l<(p^{2k}+1)/2$, of a p-ary m-sequence, s(t) of period $p^n-1$. In this paper, it is shown that the cross-correlation function between s(t) and s(dt+l) takes the values in $\{-1,-1{\pm}\sqrt{p^n},-1+2\sqrt{p^n}\}$ and their, cross-correlation distribution is also derived.

• Food Science and Biotechnology
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• v.18 no.1
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• pp.207-212
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• 2009

Celandine (Chelidonium majus, family Papaveraceae) is an herb used extensively in traditional Korean medicine. To investigate its antioxidant and antiproliferative activities, the methanolic extract of celandine was introduced. The antioxidant properties of the extract were tested using various in vitro systems, including hydroxyl radical scavenging assay, DNA damage protection assay, 1,1-diphenyll-2-2-pricylhydrazyl (DPPH) free radical scavenging activity, metal chelating activity, and reducing power assay. The extract exhibited stronger antioxidant activity ($IC_{50}=7.92{\mu}g/mL$) against hydroxyl radicals in the Fenton system than butylated hydroxyanisole ($IC_{50}=51.46{\mu}g/mL$) and $\alpha$-tocopherol ($IC_{50}=67.48{\mu}g/mL$). Likewise, damage to the plasmid pBR 322 induced by hydroxyl radicals was found to be protected by the extract at a concentration of $400{\mu}g/mL$. Cellular proliferation and the induction of apoptosis were also examined by a cellular proliferation assay, flow cytometry, and mRNA expression analysis. Taken together, the extract significantly inhibited the growth of HT-29 cells in a concentration- and time-dependent manner, and gradually increased both the proportion of apoptotic cells and the expression of caspase-3. Overall, our research suggests that celandine possesses antioxidant and antiproliferative properties.

• Bulletin of the Korean Mathematical Society
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• v.22 no.1
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• pp.7-10
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• 1985

For each a.mem.S and f.mem.m(S), denote by $l_{a}$ f(s)=f(as) for all s.mem.S. If A is a norm closed left translation invariant subalgebra of m(S) (i.e. $l_{a}$ f.mem.A whenever f.mem.A and a.mem.S) containing 1, the constant ont function on S and .phi..mem. $A^{*}$, the dual of A, then .phi. is a mean on A if .phi.(f).geq.0 for f.geq.0 and .phi.(1) = 1, .phi. is multiplicative if .phi. (fg)=.phi.(f).phi.(g) for all f, g.mem.A; .phi. is left invariant if .phi.(1sf)=.phi.(f) for all s.mem.S and f.mem.A. It is well known that the set of multiplicative means on m(S) is precisely .betha.S, the Stone-Cech compactification of S[7]. A subalgebra of m(S) is (extremely) left amenable, denoted by (ELA)LA if it is nom closed, left translation invariant containing contants and has a multiplicative left invariant mean (LIM). A semigroup S is (ELA) LA, if m(S) is (ELA)LA. A subset E.contnd.S is left thick (T. Mitchell, [4]) if for any finite subser F.contnd.S, there exists s.mem.S such that $F_{s}$ .contnd.E or equivalently, the family { $s^{-1}$ E : s.mem.S} has finite intersection property.y.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.57 no.3
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• pp.209-215
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• 2024

Ishige foliacea belongs to class Phaeophyceae and family Ishigeaceae. This study investigated the physiological activities of the Korean marine algae I. foliacea. Its solvent extracts were prepared with 70% ethanol, 80% methanol, and distilled water. The ethanol and methanol extracts had higher α-glucosidase (half-maximal inhibitory concentration, IC₅₀: 0.67-0.73 mg/mL), xanthine oxidase (IC₅₀: 0.25-0.28 mg/mL), and angiotensin-converting enzyme (IC₅₀: 25.29-38.28 ㎍/mL) inhibitory activities than those of the water extract. The ethanol and methanol extracts possessed high acetylcholinesterase inhibitory activity (IC₅₀: 0.78c0.97 mg/mL). Conversely, the water extract exhibited the highest β-secretase inhibitory activity (IC₅₀: 0.48 mg/mL). These results indicate that I. foliacea may be useful as a functional substance in food and pharmaceuticals with anti-diabetic, anti-gout, anti-hypertension, and anti-dementia properties.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.3
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• pp.408-412
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• 1997

The cytogenetic analysis of river puffer, Takifugu obscurus belongs to Family Tetraodontidae, was performed. The chromosome number of T. obscurus was 44 and the fundamental number was 64. Heteromorphic sex chromosomes were not found. The mean cellular size and nuclear size were $11.01\times7.95{\mu}m$ and $4.05\times3.15{\mu}m$, respectively. The mean surface area and volume in cell and nucleus were $68.76{\mu}m^2\;and\;366.00{\mu}m^3,\;10.06{\mu}m^2\;and\;21.36{\mu}m^3$, respectively. The number of erythrocyte of both female and male was $12\~13\times10^5/m\ell$. Gill tissues from diploid individuals had cells with one or two nucleoli. These cytogenetic studies should be used for cytotaxonomy and as a valuable estimation of polyploidy to come in T. obscurus.

• Journal of Korean Society of Forest Science
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• v.90 no.1
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• pp.105-112
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• 2001

This study was carried out to analyze the genetic variation and to estimate both heritabilities and genetic gains for height and diameter growth of 20-year-old open-pollinated progenies of Korean white pine in three different sites. For there, analysis of variance for both height and diameter growth was conducted to see if there exist significant differences among families, sites, blocks, and their interactions exist or not and to analyze the variance components for each factor. Mean height and diameter at Gapyeong site were 7.65m and 11.92cm, respectively. they were 7.42m and 11.35cm at Gwangiu site, 6.13m and 8.41cm at Youngdong site, and 7.12m and 10.68cm for the overall sites. The family No. 20 showed the most excellent growth of 7.99m in mean height and 12.14cm in mean diameter for all sites surveyed. The estimates of heritabilities for individual-tree and family were, 0.35~0.73 and 0.65~0.83 for height growth, and 0.12~0.40 and 0.46~0.75 for diameter growth, respectively. For the combined data from all the sites, the estimates of individual tree heritability were 0.60 for height and 0.20 for diameter, and those of family were 0.91 for height and 0.77 for diameter. Heritability estimates varied with testing sites, and those for height were higher than those for diameter in all sites. Given equal intensity of selection, combined selection method provided the most efficient genetic gains for both height and diameter growth.

• Journal of Microbiology and Biotechnology
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• v.18 no.2
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• pp.287-294
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• 2008

Three amino acid residues (His119, Glu164, and Glu338) in the active site of Thermus caldophilus GK24 ${\beta}$-glycosidase (Tca ${\beta}$-glycosidase), a family 1 glycosyl hydrolase, were mutated by site-directed mutagenesis. To verify the key catalytic residues, Glu164 and Glu338 were changed to Gly and Gln, respectively. The E164G mutation resulted in drastic reductions of both ${\beta}$-galactosidase and ${\beta}$-glucosidase activities, and the E338Q mutation caused complete loss of activity, confirming that the two residues are essential for the reaction process of glycosidic linkage hydrolysis. To investigate the role of His119 in substrate binding and enzyme activity, the residue was substituted with Gly. The H119G mutant showed 53-fold reduced activity on 5mM p-nitrophenyl ${\beta}$-D-galactopyranoside, when compared with the wild type; however, both the wild-type and mutant enzymes showed similar activity on 5mM p-nitrophenyl ${\beta}$-D-glucopyranoside at $75^{\circ}C$. Kinetic analysis with p-nitrophenyl ${\beta}$-D-galactopyranoside revealed that the $k_{cat}$ value of the H119G mutant was 76.3-fold lower than that of the wild type, but the $K_m$ of the mutant was 15.3-fold higher than that of the wild type owing to the much lower affinity of the mutant. Thus, the catalytic efficiency $(k_{cat}/K_m)$ of the mutant decreased to 0.08% to that of the wild type. The $k_{cat}$ value of the H119G mutant for p-nitrophenyl ${\beta}$-D-glucopyranoside was 5.l-fold higher than that of the wild type, but the catalytic efficiency of the mutant was 2.5% of that of the wild type. The H119G mutation gave rise to changes in optima pH (from 5.5-6.5 to 5.5) and temperature (from $90^{\circ}C\;to\;80-85^{\circ}C$). This difference of temperature optima originated in the decrease of H119G's thermostability. These results indicate that His119 is a crucial residue in ${\beta}$-galactosidase and ${\beta}$-glucosidase activities and also influences the enzyme's substrate binding affinity and thermostability.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.196-196
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• 2017

Oilseed crop Camelina (Camelina sativa L.) is a suitable for biodiesel production that has high adaptability under low-nutrient condition like marginal land and requires low-input cost for cultivation. Enhanced abiotic stress tolerance of Camelina is very important for oil production under the wide range of different climate. CsRCI2s (Rare Cold Inducible 2) are related proteins in various abiotic stresses that predicted to localized at plasma membrane (PM) and endoplasmic reticulum (ER). These proteins are consist of eight-family that can be divided into tail (CsRCI2D/E/F/G) and no-tail (CsRCI2A/B/E/H) type of C-terminal. However, it is still less understood the function of C-terminal tail. In this study, CsRCI2D/H genes were cloned through gateway cloning system that used pCB302-3 as destination vector. And we used agrobacterium-mediated transformation system for generation of overexpression (OX) transformants. Overexpression of target gene was confirmed using RT-PCR and segregation ratio on selection media. We analyzed physiological response in media and soil under abiotic stresses using CsRCI2D and CsRCI2H overexpression plant. To compare abiotic stresses tolerance, wild type and CsRCI2D/H OX line seeds were sown on agar plate treated with various NaCl and mannitol concentration for 7 days. In the test of growth rate under abiotic stress on media, CsRCI2H OX line showed similar to NaCl and mannitol stress. In the other hand, CsRCI2D OX line showed to be improved stress tolerance that especially increased in 200mM NaCl but was similar on mannitol media. In greenhouse, WT and CsRCI2D/H OX lines for physiological analysis and productivity under abiotic stresses were treated 100, 150, 200mM NaCl. Then it was measured various parameters such as leaf width and length, plant height, total seed weight, flower number, seed number. CsRCI2H OX line in greenhouse did not show any changes in physiological parameters but CsRCI2D OX line was improved both physiological response and productivity under NaCl stress. Among physiological parameters of CsRCI2D OX line under NaCl stress, leaf length and width were observed shorter than WT but it were slightly longer than WT in 200mM NaCl stress. Furthermore, total seed weight of CsRCI2D OX line under stress displayed to decrease than WT in normal condition, but it was gradually raised with increasing NaCl stress then more than WT relatively. These results suggested CsRCI2D might be contribute to improve abiotic stress tolerance. However, function of CsRCI2H is need to more detail study. In conclusion, overexpression of CsRCI2s family can generate various environmental stress tolerance plant and may improve crop productivity for bio-energy production.

• Journal of the East Asian Society of Dietary Life
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• v.24 no.6
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• pp.739-747
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• 2014

The aim of the study was to determine the effects of Ixeris dentata extract (IDE) on anticancer activity in human breast cancer MDA-MB-231 cells at both cellular and molecular levels. The cells were cultured in the presence of 0, 20, 30 and $40{\mu}g/mL$ Ixeris dentata extract for 24 hours, respectively. At the end of culture, cytochemical analyses for MTT activity, trypan blue dye exclusion, Annexin V-FITC Apoptosis, and radical oxygen species (ROS) were conducted. RT-PCR was also performed to determine whether or not alterations in cell viability affect the Bax/Bcl-2 ratio. MTT assay showed that relative cell viability decreased in a dose-dependent manner (p<0.05). Reduction of cell viability matched well with increased cell membrane permeability as determined by trypan blue dye exclusion test (p<0.05). The rates of intracellular ROS also increased in a similar manner to those of TB-stained cells. There was an associated shift of apoptotic cells from early to late apoptosis between the 30 and $40{\mu}g/mL$. Bax/Bcl-2 ratio significantly increased along with significant decreases in Bcl-2 expression between 30 and $40{\mu}g/mL$ groups (p<0.05). In conclusion, anticancer activity of Ixeris dentata extract is modulated by a reduction in cell viability along with increased membrane permeability, leading to ROS accumulation within cells, and subsequently cell death through an apoptotic pathway that involves Bax and Bcl-2 in human breast cancer MDA-MB-231 cells.

• Research in Community and Public Health Nursing
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• v.11 no.2
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• pp.470-485
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• 2000

The purpose of this study was to investigate health promotion lifestyle profiles of the Korean elderly in Japan. The subjects of this study were 202 elders in Aichi prefecture. The method of this study questionnaires interviews, and a self-administered questionnaire from the 1st to the 20th of April in 2000. This study tool was based on Walker et al. (1987)'s Health Promotion lifestyle Profiles (HPLP). Analysis of the data used an SPSS/pc+ WIN 9.0 program. Data analyses were conducted by using frequency, percentage, t-test, ANOVA. Pearson correlation, Kendal tau and Stepwise Multiple Regression. The results were as follows: 1. The average score of Health Promotion Lifestyle Profiles of Korean elderly in Japan was 2.72(SD=0.40). On the sub-scale of Health Promotion Lifestyle Profiles: nutrition(M = 3. 06), self-actualization(M =2.99), interpersonal support (M=2.81), stress management (M=2.68) revealed higher scores, whereas the scores of health responsibility(M=2.52), and exercise(M=1.92) were lower. 2. Health Promotion Lifestyle Profiles were significantly different by age (F = 11. 02, p = .000), religion(F=2.96, p=.033), occupation(F=6.91, p=.000), living family status(F=13.07, p=.000), living family number(F=11.74, p=.000), monthly pocket money(F=18.79, p=.000), the source of pocket money (F=7.18, p=.000), and the length of residence(F=9.79, p=.000). 3. Health Promotion Lifestyle Profiles was significantly predicted by monthly pocket money($r^2$ change =0. 188, p=.000), sex($r^2$ change= 0.066, p= .000), the length of residence($r^2$ change = 0.059, p = .000), the source of pocket money($r^2$ change=0.036, p= .000), age ($r^2$ change=0.018, p=.000). These variables showed a positive correlation with health promotion lifestyle profiles of the Korean elderly in Japan. These variables explained 36.7% of the variance of Health Promotion Lifestyle Profiles. On the basis of the above findings, Health Promotion Lifestyle Profiles programs for Korean elderly in Japan needs be to developed with Japanese social-culture perspectives and needs to develop an intervention method to improve Health Promotion Lifestyle Profiles. It is also suggested that further research is required.