• Journal of Photoscience
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• v.7 no.2
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• pp.73-78
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• 2000

To analyze molecular traits determining pigmentation between Pharbitis nill violet and white, Amplified Fragment Length Polymorphism(AFLP) and Differential Display Reverse Transcription(DDRT) experiments were carried out with either genomic DNAs or total RNAs isolated from both plants. Results of AFLP experiment in combination of 8 EcoRⅠ primers with 6 MseⅠ primers showed 41 violet-and 60 white-specific DNA bands. In the subsequent experiment, 22 violet-and 22 white-specific DNA fragments were amplified by PCR with DNAs eluted. The sizes of the fragments range from 200 to 600bp. DDRT using total RNA produced 19 violet-and 17 white-specific cDNA fragments, ranging from 200 to 600bp. The fragments obtained by both AFLP and DDRT had been cloned into pGEM T-easy vector, amplified and subjected to the nucleotide sequence analyses. As a result of Blast sequence analysis, most of them sequenced up to date showed no similarity to any Known gene, while few has similarity to known animal or plant genes. An AFLP clone V6, for example, has a strong sequence similarity to the human transcription factor LZIP-alpha mRNA and a DDRT clone W19 to Solanum tuberosum 3-hydroxy-3-methylglutaryl coenzyme A reductase mRNA.

• Journal of Computing Science and Engineering
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• v.9 no.1
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• pp.1-8
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• 2015

Accessing and searching in a sequence of numbers are fundamental operations in computing that are encountered in a wide range of applications. One of the applications of the problem is cryptanalytic time-memory tradeoff which is aimed at a one-way function. A rainbow table, which is a common method for the time-memory tradeoff, contains elements from an input domain of a hash function that are normally sorted integers. In this paper, we present a practical indexing method for a monotonically increasing static sequence of numbers where the access and search queries can be addressed efficiently in terms of both time and space complexity. For a sequence of n numbers from a universe $U=\{0,{\ldots},m-1\}$, our data structure requires n lg(m/n) + O(n) bits with constant average running time for both access and search queries. We also give an analysis of the time and space complexities of the data structure, supported by experiments with rainbow tables.

• Microbiology and Biotechnology Letters
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• v.24 no.5
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• pp.553-559
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• 1996

We have designed nucleotide sequences of hEGF structural gene to eliminate the N-terminal methionine residue incorporated during the translation initiation step, and constructed recombinant human epidermal growth factor (rhEGF) secretion plasmids pYHB101, and pYHB2 in which pelB signal sequence-hEGF gene was expressed under the control of the T7, and tac promoter, respectively. We also constructed pYHB1 vector which contains rhEGF gene controlled by T7 promoter. The transformant with pYHB101 showed relatively slow growth pattern compared to the transformant with pYHB1. However, we observed that the transformant with pYHB101 secreted rhEGF of 13 mg/l significantly after 5 hr induction with 1 mM IPTG and that the T7 promoter was more effective than tac promoter when connected to pelB signal sequence. The amount of rhEGF was 14 mg/l under the sub-optimized condition.

• Journal of Satellite, Information and Communications
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• v.5 no.2
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• pp.64-68
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• 2010

In this paper, we studied the novel synchronization algorithm for cooperative communication system over fading. We research mainly on the decode-and-forward scheme. Also, we inserted spreading sequence in origin data frame to control efficiently data synchronization. In mobile station, inserted spreading sequence in data frame passed through the corelation process. We had decide the delay value of received data through result of correlation process. In simulation, We applied that channel gain of three node had different value in various fading environment. Finally we will be possible to control the received data synchronization using result of corelation value in each node between relay to mobile station and base station to mobile station. The results of this paper can be applicable to the cooperative systems.

• Microbiology and Biotechnology Letters
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• v.31 no.1
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• pp.36-41
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• 2003

In order to increase the production and secretion rate of mouse salivary $\alpha$-amylase from Saccharomyces cerevisiae, various experiments were attempted. A plasmid pCNNinv (AMY) was constructed by the substitution of ADCl promoter and native signal sequence of mouse salivary $\alpha$-amylase cDNA gene with PRBI promoter and yeast invertase leader sequence, which resulted in 25% increase in the production of $\alpha$-amylase in the culture medium. The respiratory deficient transformant carrying pCNNinv (AMY) were obtained by treating yeast cells with ethidium bromide, and the $\alpha$-amylase activities in the culture brothes of the respiratory-deficient transformants were 5-8 times higher than that of parental wild type strain. $\alpha$-Amylase activity was also increased 3 times when the 0.015% (w/v) of 2-mercaptoethanol was added to the culture medium.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• v.20 no.2
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• pp.187-198
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• 1990

Examinations of the temporomandibular joints were performed on a 1.5 Tesla magnetic resonance (MR) system. An MR surface receiver coil 3 inch in diameter was placed on plastic frame, the patient's head being placed in the frame so that the coil was pressed against the temporal region. In taking advantage of the magnetic resonance imaging that has been studied briskly till now, author obtained the images of parasagittal and paracoronal planes about the temporomandibular joint by using MPGR (Multi-Planar Gradient Recalled), GRASS (Gradient Recalled Acquisition in the Steady State), and CSMEMP (Contiguous Slice Multiple Echo, Multi-Planar), that differ from the Spin Echo pulse sequence which the previous authors used. Five subjects with no symptoms of temporomandibular joint pain and dysfunction were studied. The plane images obtained by these methods were compared with those by Spin Echo pulse sequence. The results were as follows: 1. The optimal repetition times (TR) and echo times (TE) for T.M.J. image were; a. 400 msec and 18 msec in PMGR pulse sequence. b. 40 msec and 12 msec in GRASS pulse sequence. c. 700 msec and 30 msec in CSMEMP pulse sequence. d. 500 msec and 20 msec in Spin Echo pulse sequence. 2. When the MPGR pulse sequence was using, T2-weighted image was obtained in very short time. On the image of the paracoronal plane by GRASS pulse sequence, meniscus showed the moderate signal intensity, and the meniscus and its anteromedial, posterolateral attachments were observed definitely with gray color. 4. The signal intensity of Spin Echo pulse sequence was equal to that of CSMEMP pulse sequence, but the image by CSMEMP pulse sequence showed relatively lower level in its resolution.

• Journal of Korean Society for Quality Management
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• v.17 no.1
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• pp.69-81
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• 1989

This study is concerned with a job sequencing method using the concept of sampling technique in the case of Job Shop. This is the follow study of Kang and Ro (1988) which examined the possibility of application of sampling technique to determine the Job Sequence in the case of Flow Shop. Not only it is very difficult, but also it takes too much time to develop the appropriate job schedules that satisfy the complex work conditions. The most job sequencing algorithms have been developed to determine the best or good solution under the special conditions or assumptions. The application areas of these algorithms are also very narrow, so it is very hard to find the appropriate algorithm which satisfy the complex work conditions. In this case it is very desirable to develop a simple job sequencing method which can select the optimal job sequence or near optimal job sequence with a little effort. This study is to examine the effect of sampling job sequencing which can select the good job of 0.01%~5% upper good group. The result shows that there is the sets of 0.05%~23% job sequence group which has the same amount of performance measure with the optimal job sequence in the case of experiment of N/M/G/$F_{max}$. This indicates that the sampling job sequencing method is a useful job sequencing method to find the optimal or good job sequence with consuming a small amount of time. The results of ANOVA show that the only one factor, number of machines is the significant factor for determining the job sequence at ${\alpha}=0.01$. It takes about 10 minutes to compare the number of 10,000 samples of job sequence by personal computer and it is proved that the selection rate of the same job sequence with optimal job sequence is 23.0%, 3.9% and 0.065% in the case of 2 machines, 3 machines and 4 machines, respectively. The area of application can readily be extended to the other work condition.

• Journal of the Korean Society for Precision Engineering
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• v.11 no.4
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• pp.77-87
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• 1994

This paper deals with an automated forming sequence design system by which designers can determine desirable operation sequences even if they have little experience in the design of cold forging process. The forming sequence design in the cold forging is very important and requires many kinds of technical and empirical knowledge. They system isproposed, which generates forming sequence plans for the multistage cold forging of axisymmtrical solid products. Since the process of metal forming can be considered as a transformation of geometry, treatment of the geometry of the product is a key in planning process. To recognize the geometry of the product section, section entity representation and primitive geometries were used. Section entity representation can be used for the calculation of maximum diameter, maximum height, and volume. Forming sequence for the part can be determined by means of primitive geometries such as cylinder, cone, convex, and concave. By utilizing this geometrical characteristics (diameter, height, and radius), the product geometry is expressed by a list of the priitive geometries. Accordingly the forming sequence design is formulated as the search problem which starts with a billet geometry and finishes with a given product one. Using the developed system, the sequence drawing with all dimensions, which includes the proper sequence of operations for the part, is generated under the environment of AutoCAD. Based on the results of forming sequence, process variables(strain, punch pressure, die inner pressure, and forming load) are determined.

• Journal of The Korean Astronomical Society
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• v.34 no.3
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• pp.143-147
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• 2001

UBVI CCD photometry of open cluster NGC 2324 is presented. C-M diagrams of this cluster show well-defined main sequence with a red giant clump centered at B - V =1.05, V =13.45. We derived the major cluster characteristics; E(B - V)=0.17$\pm$0.12 from color-color diagram and mean color of red giant clump stars, (m - M)o=13.1$\pm$0.1 from zero age main sequence fitting, and [Fe/H]$\~$-0.32 from comparison the theoretical model developed by Bertelli et al. (1994) to the observed C-M diagrams. We estimate the age of NGC 2324 to be log t$\~$8.8 by applying isochrone fitting and morphological age index method.

• Korean Journal of Medicinal Crop Science
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• v.13 no.5
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• pp.293-297
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• 2005

A thoredoxin (CTRX) gene was cloned and characterized from a full length cDNA library prepared from taproot of three-year old Codonopsis lanceolata. A CTRX was 666 nucleotides long and has an open reading frame of 372 bp with 124 amino acid residues (pI = 4.92). The deduced amino acid sequence of the CTRX matched to the previously reported plant thioredoxin h genes. The deduced amino acid sequence of CTRX exhibited the similarity of 33-67% among previously registered thioredoxin genes. The expression of CTRX in leaves of Codonopsis lanceolata was increased by wounding and 1 mM $H_2O_2$, but decreased by 0.1 mM cadmium.