• 한국환경보건학회지
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• 제39권1호
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• pp.48-55
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• 2013

Objective: The use of nanoparticle products is expected to present a potential harmful effect on consumers. Also, the lack of information regarding inhaled nanoparticles may pose a serious problem. In this study, we addressed this issue by studying pulmonary toxicity after nasal instillation of Al-NPs in SD rats. Methods: The animals were exposed to Al-NPs at 1 mg/kg body weight (low dose), 20 mg/kg body weight (medium dose) and 40 mg/kg body weight (high dose). To determine pulmonary toxicity, bronchoalveolar lavage (ts.AnBAL) fluid analysis and histopathological examination were conducted in rats. In addition, cell viability was investigated at 24 hours after the treatment with Al-NPs. Results: BAL fluid analysis showed that total cells (TC) count and total protein (TP) concentrations increased significantly in all treatment groups, approximately two to three times. Also, lactate dehydrogenase (LDH) and cytokines such as TNF-alpha and IL-6 dose-dependently increased following nasal instillation of Al-NPs. However, polymorphonuclear leukocytes (PMNs) levels showed no significant changes in a dose dependant manner in BAL fluid. In the cytotoxicity analysis, the treatment of Al-NPs significantly and dose-dependently induced cell viability loss (20 to 30%) and damage of cell membrane (5 to 10%) in rat normal lung epithelial cells (L2). Conclusions: Our results suggest that inhaled Al-NPs in the lungs may be removed quickly by alveolar macrophages with minimal inflammatory reaction, but Al-NPs have the potential to affect lung permeability. Therefore, extensive toxicity evaluations of Al-NPs are required prior to their practical application as consumer products.

• 한국환경성돌연변이발암원학회지
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• 제26권4호
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• pp.119-124
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• 2006

There are $10{\sim}30%$ polyphenol and $2{\sim}4%$ caffeine in green tea. Caffeine is a kind of alkaloid containing nitrogen which cause stimulation, impatience, headache, insomnia, low birth weight infant. Because of these negative effect, decaffeined beverage came out and decaffeined coffee already have a big market since 1970s. Having proving the physiologic functions of green tea, high consumption of coffee is shifting to green tea. Because of the carcinogenic effect of the organic solvents, decaffeine processing with supercritical carbon dioxide has industrialized and have an advantage in environment-friendly and minimized flavor loss. Decaffeined green tea using supercritical carbon dioxide is considered to be safe but there are not enough study. We investigated the chromosome aberration test with mammalian cell line, CHL. When the cells were treated with 5000, 2000, 1000 ${\mu}g/ml$ and compared with the negative controls, there were no significant(P>0.05) increased chromosome aberration. Same results was observed when adding S9 mixture or not. As a result, water extract of decaffeined green tea using supercritical carbon dioxide does not induce chromosome aberration.

• 대한예방한의학회지
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• 제14권1호
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• pp.111-123
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• 2010

Backgrounds : Stroke is characterized by loss of brain functions due to a disturbance in the blood vessels supplying blood to the brain, and classified into hemorrhage and ischemia. Stroke is known to be affected by genetic factors and other diseases such as hypertension and cardiovascular diseases. However, the distinctive association between stroke and genetic variations has not discovered yet. Objectives : This study investigated the effects of fibrinogen level and genetic variations in FGA (Fibrinogen alpha chain) gene on stroke in Korean stroke patients and controls. Methods : DNA samples from 674 stroke patients diagnosed by Oriental medical hospitals and 267 controls were used in this study. Two common single nucleotide polymorphism(SNP) with high minor allele frequency(MAF), rs2070011G/A of promoter region and nonsynonymous rs6050A/G of exon 5 in FGA gene, were targeted for Taqman genotyping. Because the TOAST classification is important to the factors and symptoms of stroke, ischemic patients were further classified into five subtypes using diagnosis and clinical data. One-way ANOVA and chi-square test were used for clinical data and genetic association, respectively. Haploview v4.1 program was used for linkage disequilibrium(LD), haplotype and haplotype block analysis. Results : The levels of red blood cells and fibrinogen from clinical data were shown to be significant factors for the sub-groups of TOAST classification. No significant associations of stroke, hemorrhage, ischemic and subtypes of TOAST with rs2070011 and rs6050 of FGA gene were found(P > 0.05). However, rs2070011 in promoter region and nonsynonymous rs6050 in exon 5 which produce the amino acid change from threonine to alanine showed a haplotype block and three haplotypes of A-G, G-A, A-A, suggesting that rs2070011 and rs6050 might be co-segregated in generic recombination. Although A-A haplotype of stroke patients showed 64-69% low frequency compared to controls, there was no significant association between stroke and haplotype(P > 0.05). Conclusion : This study showed that there was no significant association between stroke and two SNP of rs2070011G/A and nonsynonymous rs6050A/G in FGA gene. However, these two SNP compose a haplotype block and three haplotypes of A-G, G-A, A-A. This finding suggests that rs2070011 and rs6050 are so close as to be positioned as linkage disequilibrium. Nevertheless, no significant association between haplotypes and stroke was found.

• 한국식품과학회지
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• 제29권6호
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• pp.1196-1201
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• 1997

저염 멸치젓 생산 공정 개발을 위한 기초 실험으로서 brining 과정 증 멸치육의 이화학적 변화를 측정하였다. 10%, 15%, 20% 염용액 및 포화식염수를 사용하여 $15^{\circ}C$ 온도 조건에서 brining 하였을 때 초기의 염침투 특성은 first order kinetics에 적용할 수 있었으며 이때의 rate constant는 각각 0.018, 0.030, 0.039, 0.051를 나타내 염용액의 염농도가 증가할수록 초기의 염침투 속도는 급격히 증가하는 것으로 나타났다. 염지기간 중 멸치육 단백질의 분해는 염용액의 염도가 낮을수록 빠르게 진행되었으며 포화식염수를 사용하여 brining 하였을 때는 단백질 분해가 매우 더디게 진행됨을 알 수 있었다. Brining 중 멸치육의 수분함량의 변화를 측정하였을 때 10% 식염수를 사용하였을 경우 74%의 수분함량을 가지고 있었으며 20% 식염수일 경우 65%의 수분함량을, 포화식염수를 사용하였을 경우 58%의 수분함량을 가지고 있는 것으로 나타나 염용액의 염도가 증가할수록 탈수되는 수분의 양이 증가하여 낮은 수분함량을 가지는 것으로 나타났다. Brining 중 멸치육의 무게변화를 측정하였을 때 10% 식염수를 사용하였을 경우 멸치육의 무게는 완만히 증가하여 72시간 후에 10% 정도 증가하는 것으로 나타났으며 20% 염용액 및 포화식염수를 사용하였을 경우 감소하는 경향을 나타내었다. 포화식염수를 사용하여 $15^{\circ}C$에서 brining 시 고형분의 용출량을 측정하였을 때 초기에는 급격히 고형분이 용출되어 36시간 이후에 평형에 도달하였는데 이때의 감량 수준은 원료 멸치육의 무게의 30%감량수준이었다.

• 한국정보전자통신기술학회논문지
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• 제13권3호
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• pp.219-225
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• 2020

인구고령화에 따라 노인의 근골격계질환의 유병률이 계속해서 증가하고 있다. 또한 근골격계질환은 2015년 기준으로 산재보험 보상급여에서 업무상질병 중 상위 세 번째로 사회 및 경제적 손실을 초래한 질환으로서 근로자들에게도 많이 발생하는 질병이다. 본 연구에서는 허리의 근골격계질환의 예방과 치료에 보조할 수 있는 조끼를 제작하여 실험을 통해 그 효과의 유무를 확인하고자 한다. 허리부분의 질환은 코어근육과 밀접한 연관이 있다. 따라서 제작한 조끼는 코어근육을 지지해 주는 형태로 제작하였다. 효과 검증을 위해 조끼의 착용 전과 후에 허리 신전 운동인 멕켄지 운동을 10회 실시하면서 코어근육에 해당되는 배속빗근(내복사근, Internal oblique), 배곧은근(복직근, Rectus abdominis), 척추세움근(척추기립근, Erector spinae)의 근전도 데이터를 수집하고 수집한 데이터를 근전도 분석 프로그램인RM-3를 통해 분석하여 각 근육의 10회 측정 시 RMS값을 구하고 그 평균값을 비교하는 실험을 진행했다. 데이터비교 이후 유의성 검증을 위해 PASW ver18.0을 통해 측정결과의 기술통계치 산출과 평균차를 검증하고, 실험 결과치에 대응표본 T검정을 시행하여 통계 처리를 했다. 통계분석의 유의 수준은 α=.05로 설정하고 통계처리를 진행하였다.

• 한국안전학회지
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• 제31권3호
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• pp.143-155
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• 2016

Recently, a massive loss of life and property is occurring in Korea due to traffic accidents, with the rapid increase in cars. For improvement of traffic safety, the Korea Transportation Safety Authority intensively analyzes accident data in local governments with low traffic safety index, performs a field investigation to extract problems and offers local governments improvements for problems, by conducting the 'Special Survey of Actual Conditions of Traffic Safety' each year, starting 2008. But local governments cannot strongly push forward the improvement projects due to the limited budget and the uncertainty of the improvement plan effects. Therefore, this study suggested a model which applied the Utility concept to the AHP theory, in order to efficiently decide a priority of the improvement plans in accident black spots in consideration of the limited budget of local governments. The number of accidents in each spot for improvement and accident severity, traffic volume, pedestrian volume, the improvement project cost and the accident reduction effect were chosen as evaluation factors for deciding a priority, and data about the improvement plan costs and the accident reduction effects, traffic accidents and traffic volume in the spots to undergo the special research on the real condition of traffic accident in the past were collected from the existing studies. Then, regression analysis was carried out and the Utility Curve of each evaluation factor was computed. Based on the AHP analysis findings, this study devised a priority decision method which calculated the weight and the utility function of each evaluation factor and compared the total utility values. The AHP analysis findings showed that among the evaluation factors, accident severity had the biggest importance and it was followed by the improvement plan cost, the number of accidents, the improvement effect, traffic volume and pedestrian volume. The calculated utility function shows a rise in utility, as the variables of the 5 evaluation factors; the number of accidents, accident severity, the improvement plan effect, traffic volume and pedestrian volume increase and a fall in utility, as the variables of the improvement plan cost increase, since the improvement plan cost is included in the budget spent by a local government.

• 한국통신학회논문지
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• 제32권2C호
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• pp.95-112
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• 2007

다중 안테나 기술의 공간 다중화 방식은 다수의 송신 안테나가 서로 다른 데이터를 전송함으로써 시스템의 동일한 대역폭을 이용하여 높은 데이터 전송률을 제공하는 기술로서, 심볼간 간섭 (ISI : Inter-Symbol Interference)과 주파수 선택적 페이딩 (Frequency Selective Fading)에 강인한 OFDM (Orthogonal Frequency Division Multiplexing)과 함께 사용된다. 하지만, OFDM은 고속 이동환경에서 서브 캐리어간의 직교성이 유지되지 않아 서브캐리어간의 간섭 (ICI : Inter-Carrier Interference)으로 인하여 시스템의 성능 열화가 발생한다. 본 논문에서는 ICI와 다중 안테나간의 상관도에 의한 CAI (Co-Antenna Interference)의 발생 원인과 그에 따른 성능 열화를 해석적으로 분석하고, 고속 이동환경에서 MIMO-OFDM 수신기의 QoS를 만족시키기 위한 ICI의 제거 방법으로 저복잡도의 HIC와 데이터 전송률을 저하시키지 않는 CIR (Channel Impuse Response) 추정 방법을 제안한다. 그리고 SCM-E Sub-urban Macro MIMO 채널에서의 모의 실험을 통해, ICI와 CAI에 의한 성능 열화 분석 결과와 모의 실험 결과가 일치하는 것을 검증하고, 고속 이동환경에서 HIC를 적용한 MIMO-OFDM 시스템의 성능이 향상되는 것을 보인다.

• 정보처리학회논문지:컴퓨터 및 통신 시스템
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• 제9권3호
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• pp.45-58
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• 2020

클라우드 서비스와 모바일 기기의 대중화로 네트워크 트래픽이 계속 증가하고 있는 현재 추세에 LAN 대역폭에 비해 WAN 대역폭이 아주 낮다. WAN 환경에서는 전송 프로토콜, 패킷 손실, 네트워크 대역폭 한계 때문에 생기는 성능 문제를 극복하는 WAN 최적화기가 필요하다. 본 논문에서는 오픈소스 WAN 최적화기인 WANProxy의 데이터 중복제거 알고리즘을 분석하고 성능을 네트워크 대기시간 및 WAN 대역폭 관점에서 평가한다. 또한, WANProxy에 추가로 zstd를 적용하는 2단계 압축을 적용할 경우의 성능을 평가한다. 또한, WANProxy의 데이터 중복 제거 방법을 개선한 새로운 방법을 제안하고 성능 개선 효과를 평가한다. 데이터 세그먼트 크기를 2048바이트로 하고 Silesia의 12개 데이터 파일을 이용한 성능 실험을 수행한다. 실험 결과에 의하면, WANProxy에 의한 평균 압축률이 150.6이고 네트워크 대기시간 평균 감소율은 10 Mbps WAN 환경에서는 95.2%, 100 Mbps WAN 환경에서는 60.7%가 된다. WANProxy에 추가로 zstd를 적용하는 방법은 WANProxy를 적용하는 경우와 비교할 때 압축률이 평균 33% 증가하지만 네트워크 대기시간이 10 Mbps WAN 환경에서는 평균 2.1%, 100 Mbps WAN 환경에서는 평균 5.2% 각각 증가한다. 본 논문에서 제안한 개선 방법을 WANProxy에 적용한 경우는 기존의 WANProxy와 비교할 때 압축률이 평균 34.8% 증가하고 네트워크 대기시간이 10 Mbps WAN 환경에서는 평균 13.8%, 100 Mbps WAN 환경에서는 평균 12.9% 각각 감소한다. 성능 분석 결과에 의하면, WAN 대역폭이 10 Mbps 이하인 환경에서 WANProxy를 적용할 경우 네트워크 대기시간과 WAN 대역폭 관점에서 성능 개선 효과가 아주 우수하고 WAN 대역폭이 100 Mbps 환경에서도 우수하다.

• 센서학회지
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• 제19권5호
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• pp.361-368
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• 2010

ACROSS device, which is composed of an implantable microphone, a signal processor, and a vibrating transducer, is a fullyimplantable middle ear hearing device(F-IMEHD) for the recovery of patients with hearing loss. And since a microphone is implanted under skin and tissue at the temporal bones, the amplitude of the sound wave is attenuated by absorption and scattering. And the vibrating transducer attached to the ossicular chain caused also the different displacement from characteristic of the stapes. For the gain control of auditory signals, most of implantable hearing devices with the digital audio signal processor still apply to fitting rules of conventional hearing aid without regard to the effect of the implanted microphone and the vibrating transducer. So it should be taken into account the effect of the implantable microphone and the vibrating transducer to use the conventional audio fitting rule. The aim of this study was to measure gain characteristics caused by the implanted microphone and the vibrating transducer attached to the ossicle chains for the gain compensation of ACROSS device. Differential floating mass transducers (DFMT) of ACROSS device were clipped on four cadaver temporal bones. And after placing the DFMT on them, displacements of the ossicle chain with the DFMT operated by 1 $mA_{peak}$ current was measured using laser Doppler vibrometer. And the sensitivity of microphones under the sampled pig skin and the skin of 3 rat back were measured by stimulus of pure tones in frequency from 0.1 to 8.9 kHz. And we confirmed that the microphone implanted under skin showed poorer frequency response in the acoustic high-frequency band than it in the low- to mid- frequency band, and the resonant frequency of the stapes vibration was changed by attaching the DFMT on the incus, the displacement of the DFMT driven with 1 $mA_{rms}$ was higher by the amount of about 20 dB than that of cadaver's stapes driven by the sound presssure of 94 dB SPL in resonance frequency range.

• Journal of Microbiology and Biotechnology
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• 제27권9호
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• pp.1701-1710
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• 2017

Classical swine fever virus (CSFV) is the etiologic agent of classical swine fever, a highly contagious disease that causes significant economic losses to the swine industry. The lapinized C-strain, a widely used vaccine strain against CSFV, has low growth efficiency in cell culture, which limits the productivity in the vaccine industry. In this study, a recombinant virus derived from C-strain was constructed and subjected to continuous passaging in PK-15 cells with the goal of acquiring a high progeny virus yield. A cell-adapted virus variant, RecCpp80, had nearly 1,000-fold higher titer than its parent C-strain but lost the ability to induce fever in rabbits. Sequence analysis of cell-adapted RecC variants indicated that at least six nucleotide changes were fixed in RecCpp80. Further adaption of RecCpp80 variant in swine testicle cells led to a higher virus yield without additional mutations. Introduction of each of these residues into the wild-type RecC backbone showed that one mutation, M979R (T3310G), located in the C-terminal region of E2 might be closely related to the cell-adapted phenotype. Rabbit inoculation revealed that $RecCpp40_{+10}$ failed to induce fever in rabbits, whereas $RecCpp80_{+10}$ caused a fever response similar to the commercial C-strain vaccine. In conclusion, the C-strain can be adapted to cell culture by introducing specific mutations in its E2 protein. The mutations in RecCpp80 that led to the loss of fever response in rabbits require further investigation. Continuous passaging of the C-strain-based recombinant viruses in PK-15 cells could enhance its in vitro adaption. The non-synonymous mutations at 3310 and 3531 might play major roles in the enhanced capacity of general virus reproduction. Such findings may help design a modified C-strain for improved productivity of commercial vaccines at reduced production cost.