Environmental Mutagens and Carcinogens (한국환경성돌연변이발암원학회지)

Korean Environmental Mutagen Society (한국환경성돌연변이발암원학회)
권호 표지

Chromosome Aberration Test of Water Extract of Decaffeined Green Tea using Supercritical Carbon Dioxide with Mammalian cell line

초임계이산화탄소를 이용한 탈카페인녹차 열수추출물의 포유동물 세포주를 이용한 염색체이상시험

  • Koo, Yun-Chang (Department of food science and technology College of Life science and biotechnology, Korea University) ;
  • Lee, Hyun-Sun (Department of food science and technology College of Life science and biotechnology, Korea University) ;
  • Park, Byung-Gyu (Department of food science and technology College of Life science and biotechnology, Korea University) ;
  • Kim, Eun-Jin (Department of food science and technology College of Life science and biotechnology, Korea University) ;
  • Lee, Sun-Joo (Department of food science and technology College of Life science and biotechnology, Korea University) ;
  • Kim, Kyoung-Hoen (Department of food science and technology College of Life science and biotechnology, Korea University) ;
  • Kim, Young-Suk (Department of Food and Nutritional Sciences, Division of Human Ecology, Ewha womans University) ;
  • Chung, Young-Shin (Medvill. Co., LTD) ;
  • Lee, Kwang-Won (Department of food science and technology College of Life science and biotechnology, Korea University)
  • 구윤창 (고려대학교 생명환경과학대학 식품과학부) ;
  • 이현순 (고려대학교 생명환경과학대학 식품과학부) ;
  • 박병규 (고려대학교 생명환경과학대학 식품과학부) ;
  • 김은진 (고려대학교 생명환경과학대학 식품과학부) ;
  • 이선주 (고려대학교 생명환경과학대학 식품과학부) ;
  • 김경헌 (고려대학교 생명환경과학대학 식품과학부) ;
  • 김영석 (이화여자대학교 생활환경대학 식품영양학과) ;
  • 정영신 ;
  • 이광원 (고려대학교 생명환경과학대학 식품과학부)
  • Published : 2006.12.31
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Abstract

There are $10{\sim}30%$ polyphenol and $2{\sim}4%$ caffeine in green tea. Caffeine is a kind of alkaloid containing nitrogen which cause stimulation, impatience, headache, insomnia, low birth weight infant. Because of these negative effect, decaffeined beverage came out and decaffeined coffee already have a big market since 1970s. Having proving the physiologic functions of green tea, high consumption of coffee is shifting to green tea. Because of the carcinogenic effect of the organic solvents, decaffeine processing with supercritical carbon dioxide has industrialized and have an advantage in environment-friendly and minimized flavor loss. Decaffeined green tea using supercritical carbon dioxide is considered to be safe but there are not enough study. We investigated the chromosome aberration test with mammalian cell line, CHL. When the cells were treated with 5000, 2000, 1000 ${\mu}g/ml$ and compared with the negative controls, there were no significant(P>0.05) increased chromosome aberration. Same results was observed when adding S9 mixture or not. As a result, water extract of decaffeined green tea using supercritical carbon dioxide does not induce chromosome aberration.

Keywords

References

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