• Clinical and Experimental Reproductive Medicine
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• v.48 no.1
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• pp.34-42
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• 2021

Objective: Studies of the effects of estrogens on the male reproductive system have emphasized the role of these hormones in male fertility. Sesame oil has many phytoestrogenic compounds and may improve male fertility. This study investigated the effects of sesame oil and different concentrations of estrogen on sperm parameters and DNA integrity in male mice. Methods: Twenty old NMRI (The Naval Medical Research Institute) male mice (40 weeks; weight, 30-35 g) were treated with sesame oil or different concentrations of estrogen (estradiol, 1 and 10 μL/kg/day) or received no treatment (controls). After 35 days, sperm parameters and DNA integrity were assessed and analyzed. Results: Sperm count, progressive motility, and morphology were decreased in the group that received 10 μL/kg of estradiol. A remarkably lower percentage of DNA fragmentation and protamine deficiency were detected in the group that received 1 μL/kg of estradiol. In the groups that received sesame oil and 1 μL/kg of estradiol, the numbers of spermatogonia and Leydig cells were higher than in controls. The combination of sesame oil and 1 μL/kg of estradiol led to improved sperm parameters and chromatin and testicular structure. Conclusion: Based on this study, consumption of sesame oil and a low concentration of estradiol may improve testicular function in older mice.

• Clinical and Experimental Reproductive Medicine
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• v.49 no.3
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• pp.210-214
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• 2022

Objective: Platelet-rich plasma (PRP) therapy has received a considerable attention as an adjunct to fertility treatments, especially in women with very low ovarian reserve and premature ovarian insufficiency. Although recent studies have demonstrated that PRP led to improvements in folliculogenesis and biomarkers of ovarian reserve, the effect of intraovarian PRP administration on embryo genetics has not been studied. Methods: We report a pilot study of patients who had preimplantation genetic testing for aneuploidy (PGT-A) before and then within 3 months following PRP administration. Twelve infertile women with at least one prior failed in vitro fertilization (IVF) cycle underwent ovarian stimulation (cycle 1) with a gentle stimulation protocol and PGT-A performed at the blastocyst stage. Following cycle 1, autologous intraovarian PRP administration was performed. Within 3 months following PRP administration, the patients underwent cycle 2 and produced blastocysts for PGT-A. The percentage of euploid embryos between both cycles was compared. Results: The mean age of all participants was 40.08±1.46 years, and their mean body mass index was 26.18±1.18 kg/m². The number of good-quality embryos formed at the blastocyst stage was similar between cycle 1 and cycle 2 (3.08±0.88 vs. 2.17±0.49, respectively; p=0.11). Among all patients in cycle 1, 3 of 37 embryos were euploid (8.11%) while in cycle 2, 11 out of 28 embryos were euploid (39.28%, p=0.002). Three clinical pregnancies were noted among this patient group. Conclusion: This novel study is the first to present an improvement in the embryo euploidy rate following intraovarian PRP application in infertile women with prior failed IVF cycles. The growth factors present in PRP may exhibit a local paracrine effect that could improve meiotic aberrations in human oocytes and thus improve euploidy rates. Whether PRP improves live birth rates and lowers miscarriage rates remains to be determined in large trials.

• Journal of Korean Society of Forest Science
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• v.82 no.3
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• pp.246-253
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• 1993

The composition of the nitrogen forms of the organic layer and mineral soil horizons of 8 differing soil type was measured to find a clue to elucidate the problem on the fertility and nitrogen availabilities of forest soils. The ratio of inorganic $N(NH_4-N+NO_3-N)$ to total N was very low in every 0 layer and its maximum was only about 2%. And the inorganic N concentration of every mineral soil horizon was low, and the maximum of its rate to N was 3.7% in surface horizon and about 4.1% in lower horizon. Organic N form of O layer were characterized by the decrease of aminoacid-N, and the increased of hexosamine-N and amide-N according to the advance of decomposing process. The ranges of hydrolyzable-N of every flesh litter, F and H layer of the selected soils were about 80-90% and they- were hardly affected by the differences of tree species and decomposing process. The organic N form of every litter was similar and it was follows : aminoacid-N was not abundant and it ranges were about 40-50%, more than half of hydrolyzable-N. The hexosamine-N was eery poor and it ranged to only 2-7%. The amide-N teas low and about 10-23%. No distinguished difference was seen between the organic N forms of the O layer and surface horizon of dry and wet soils in brown forest soils.

• Korean Journal of Plant Resources
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• v.14 no.2
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• pp.163-168
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• 2001

This study was conducted to obtain basic information on cross breeding for developing bolting resistant variety of Angelica gigas NAKAI which has been subjected to severe yield reduction resulted from flower-stalk initiation during the growth. Compatibility of selfing and/or crossing pollination among 5 local cultivars were tested. Results obtained are summarized as follows. In case of three year old plant, all cultivars emerged March 14 and bolted from May 12 to May 13. Ponghwa cultivar was flowered 3 to 5 days earlier than other cultivars. Flower duration continued 41 to 43 days. Interval of flowering date from main stem to first primary branch, from first to second or second to third primary branch was 9 to 13 days. Emergency of stamen proceeded 4~8 days of pistil initiation demonstrating that Angelica gigas NAKAI is protandrous plant. Furthermore initiation of first pistil was 1~4days later than formation of last stamen in the same flower cluster. Percent pollination of Angelica gigas NAKAI was 52% under natural condition, so called open-pollination, but bagging by enveloping flowers was as low as 0.4~2.4%. In the result of compatibility test, all local cultivars tend to show both of self-and cross-compatibility. Inpollination, ‘Muju’$\times$‘Muju’combination showed highest fertility up to 40.8% whereases cross pollination, ‘Pyeongchang’$\times$‘Inje’combination, showed 67.0% fertility.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.2
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• pp.156-160
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• 1985

24 cultivation areas at fields of the Tobacco Experiment Station in Suweon, Eumseong, and Daegu were selected to get the fertilizer response and evaluate fertility level of upland soil. Fertilizer levels treated were 0, 70, 100, and 130kg/10a of compound fertilizer (10-10-20) for tobacco. The results are as follows; 1. Fertility level could be evaluated by dry weight of tobacco loaves harvested from non-fertilized fields. 2. There was significant in yield differences between fertilizer levels of 70 and 100kg/10a but was no difference between 100 and 130kg/10a of fertilizer level. The lower fertilizer efficiencies were showed at the fields harvested high yield of tobacco leaf without fertilizer. 3. Optimum fertilizer level for non-fertilized field with leaf yield less than 208.5kg/10a was 100kg/10a, and at low productive field with dry leaf weight less than 33.3kg/10a, higher level of fertilizer, 130kg/10a, was considered to be the optimum to get the got income with tobacco cultivation.

• Journal of Animal Science and Technology
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• v.45 no.4
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• pp.517-522
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• 2003

Scrotal measurements and semen production records of 19,742 during 7 years from 63 Hanwoo breeding bulls(6${\sim}$7 years of age) were used to determined the relationship between scrotal circumference(SC) and semen production including concentration and total number of sperm. The average of SC is 41.2$\pm$4.54cm; extreme ranges between a low of 34cm to a high of 48cm. Quantity of semen, concentration and total number of sperm are 5.47$\pm$0.89ml/ejaculation, 16.16${\pm}2.78{\times}10^8$/ml and 88.47${\pm}15.24{\times}10^8$/ejaculation, respectively. SC is positively correlated with semen production(0.14 with quantity of semen and 0.15 with total number of sperm). Reproduction data from 16 breeding bulls produced 1,289 offspring from spring 1993 to spring 2000 to examined relationship between SC and reproduction. Rate of fertility and delivery are 0.80$\pm$0.13 and 0.78$\pm$0.15, respectively. Also, SC is positively correlated with delivery rate(r=0.26) and favorably related to fertility rate(r=0.39). These results, that is, - SC correlates positively with semen production and with reproduction -, suggested that SC should be considered when selecting breeding bull.

• Horticultural Science & Technology
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• v.30 no.5
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• pp.573-578
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• 2012

A total of eleven Chinese cabbage accessions were used for microspore culture and were grown to take basal data. Based on the collected data, breeding materials were chosen to develop new improved Chinese cabbage cultivars. The range of microspore-derived embryoid taken from flower buds was 1.6 to 35.4 embryoids. The embryoids from IT26110 and IT26153 among the Chinese cabbages were more than 34 per flower bud. The viability rate after cold treatment was low from 0.2 to 11.7%. The range of fertility rate was 7.7 to 58.8% in general but the IT26118, IT26122, IT26128, IT26130, and IT26164 were more than 50%. The result of their seed production ability by selfing was 11.9 seeds per siliqua in IT26128 while the others were less than 10 seeds. In the microspore culture using parents of different hereditary, the number of embryoids, the number of plants, the rate of fertility and their pure seed production ability appeared to be very different in doubled haploid lines obtained from fertile plants of Chinese cabbage.

• Korean Journal of Soil Science and Fertilizer
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• v.19 no.1
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• pp.63-69
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• 1986

1. Chemical components of fresh tobacco leaves at topping stages were affected variously by fertilizer application level. The more fertilizers were applicated, the higher nitrogen content of leaves was shown regardless of the soil fertility, but phosphorus content was not affected either by phosphorus rate or soil fertility. Potassium content was higher in the leaves grown in fertile soil than infertile at the same application rate. 2. Maturation of tobacco leaves was delayed by applying high level of nitrogen fertilizer, especially in fertile soil. The excessive accumulation of nitrogen in tobacco leaves at later stage of growth resulted in poor quality index for the high content of nicotine and low content of reducing sugar in cured leaves. 3. Nicotine content of cured leaf was increased significantly as nitrogen content increased, regardless of soil fertility, but reducing sugar content was reduced. Nicotine and reducing sugar content of cured leaf were higher in fertile than in infertile soil. 4. Resulting from the facts that nicotine contents were negatively correlated and reducing sugar contents were positively correlated with grading value (Won/Kg), authors suggested that grading index (Won/Kg) of the Office of Monopoly be based on quality index from chemical components of cured leaves.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.3
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• pp.226-235
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• 1991

This experiment was carried out to obtain the information on the crossability, germination rate, and pollen viability of the progeny from the cross between hexaploid triticale cv. Sinkihomil and five hexaploid wheat varieties. The results are summarized as follows. Seed set was 28.8 to 41.8% (ave-raged 34.1%) in the cross between triticale and wheat, which resulted in 3.61% in F$_2$(selfed F$_1$), 3.99% in F$_1$/P$_1$ 21. 9% in F$_1$/P$_2$ respectively. However, seed set was extremely low in reciprocal crosses when triticale was used as male. In the backcross, crossability was higher in F$_1$/wheat and triticale/F$_1$ than that in wheat/F$_1$ and F$_1$/triticale, respectively. Germination rate of the crossed seed was 95% in F$_1$ 66.3% in F$_2$ (selfed F$_1$), 62.0% in F$_1$/P$_1$ and 81.0% in F$_1$/P$_2$ from the cross between triticale and wheat, respectively. When triticale cv. Sinkihomil was used as male, seeds were degenerated because of the failure of endorsperm development. This might be caused by AAABBBDDR genome constitution in the cytoplasm from a hexaploid wheat. Pollen fertility of F, plant was averaged 34.1% in the cross between triticale and wheat. Significant positive correlation between the pollen fertility and seed set rate in the cross between triticale and wheat were detected.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.2
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• pp.77-83
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• 2021

Rye (Secale cereal L.) is the most tolerant to abiotic stress including low temperature, drought, and unfavorable soil conditions among the winter cereals. Rye is the rapid growth of early spring results from increasing areas for the use of the forage and green manure in the middle part of Korea. "Jungmo2509", a rye cultivar was developed by the National Institute of Crop Science (NICS), RDA in 2014. It was developed from a cross between "Olhomil", a self-compatible cultivar, and "Synthetic II", a self-incompatible line. "Jungmo2509" is an erect plant type and of a middle size, with a green leaf color, a yellowish-white colored culm, and a yellowish brown-colored, small-size grain. The heading date of "Jungmo2509" was April 23, which was 5 days later than that of "Gogu", respectively. But "Jungmo2509" showed greater resistance to lodging compared to that of the check cultivar, with similar to winter hardiness, wet injury, and disease resistance. "Jungmo2509" was a higher to than "Gogu" in terms of protein content (9.4% and 8.0%, respectively), total digestible nutrients (TDN) (55.7% and 55%, respectively). The seed productivity of "Jungmo2509" was approximately 2.08 ton 10a-1, which was 11% lower than that of the check. Almost all rye cultivars are out-crossing due to genes controlling incompatibility, but "Jungmo2509" is higher seed fertility (56%) than that of Gogu (0%). it has self-compatible genes. "Jungmo2509" is erect in plant type and resistance for lodging. Therefore, "Jungmo2509" can produce uniform seeds for processed grains of human consumption and utilize them as parents for breeding the rye hybrids with high forage yields.