• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.172-172
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• 1993

Acetaminophen(이하 AAP)은 간의 mixed function oxidase system에 의해 toxic metabolite로 대사되어 간괴사를 일으킨다. 최근에는 지금까지 임상적으로 사용되어온 한약이나 생약의 cytochrome P450의 활성과 간의 glutathion 합성에 미치는 영향에 대한 연구가 활발하게 진행되고 있다. 본 연구에서는 AAP의 간독성을 억제하는 물질을 규명하고자 민간이나 한방에서 보간 또는 해독제로 많이 사용되고 있는 생약을 screening하였다. 그 결과 간의 약물대사 효소계에 엉힝을 미치는 것으로 보고된 강활(Angelica koreana)과 민간이나 한방에서 간질환치료의 목적이나 해독제로 쓰여온 시호(Buplerum falcatum), 토복령(Smilax china) 및 금은화(Lonicera japonica)가 AAP로 유발된 간독성에 미치는 효과를 살펴보았다. 방법: 예비실험을 통해 강활, 시호, 토복령 및 금은화의 MeOH ext.가 AAP로 유도된 간독성에 방어효과가 있음을 확인하고 이를 hexane, ether, ethyl acetate 및 butanol로 계통분획하여 랫트에 전처치한 후 고농도의 AAP를 투여하여 간 손상을 일으키고 혈청중의 효소활성과 지질ㆍcholesterolㆍbilirubin함량 등을 측정하였다.

• The Korean Journal of Ecology
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• v.15 no.3
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• pp.247-255
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• 1992

In order to estimate effects of air pollution for herbs in the plnus thunbergii forests arrounding onsan industrial complex, this study was surveyed. number of species of herb also had a difference over 10 species by distance at the source of air pollution, and species diversity and evennes were also increasing at remote sites in industrial complex as woody plants. At the vicinity of industrial complex, $SDR_4$ of calamagrostis arundinatios arundinacea., miscanthus sinensis and cymbopogon tortilis var. goeringii were very high, and those of lianas as cocculus trilobus, lonicera japonica and paederia scandens, and phytolacca americana were low, but because of being higher than other sites, it was able to inferred that lianas had a tolerance to air pollution. The composition of biological spectra of herbs showed to type of $H-D_1-R_{2-3}-1$ at near site in the source of air pollution and $H-D_1-R_{1-3}-t$ at the other sites, and the propotion of lians forms were relatively high at the vicinity of industril complex.

• Journal of Wetlands Research
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• v.17 no.3
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• pp.273-282
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• 2015

This study suggested the adaptable plant species according to the change of warmth index (WI) through the Representative Concentration Pathway (RCP) 4.5 & 8.5 climate change scenarios from 2010 to 2099 in Seoul areas. From the scenario analysis results, we expected to change from the cool temperate souther forest zone to the warm temperate forest zone. We found the following adaptable 27 plant species: 6 species in the tree layer, Quercus serrata, Q. variabilis, Pinus densiflora, Q. acutissima, Styrax japonica and P. thunbergii etc.; 7 species in the shrub layer, Ligustrum obtusifolium, Lespedeza maximowiczii, Rhus trichocarpa, Callicarpa japonica, Rubus crataegifolius, Rosa multiflora, and Zanthoxylum piperitum etc.; 3 species in the herb layer, Oplismenus undulatifolius, Pteridium aquilinum var. latiusculum, and Commelina communis ect;, 11 species in the vine plants Smilax china, Cocculus trilobus, Parthenocissus tricuspidata, Lonicera japonica, Paederia scandens, Celastrus orbiculatus, Clematis apiifolia, Rubus parvifolius, Dioscorea batatas, Hydrangea serrata for. acuminata, Zelkova serrata etc.

• The Korea Journal of Herbology
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• v.29 no.2
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• pp.1-6
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• 2014

Objectives : Reactive oxygen species (ROS) are involved in a wide spectrum of diseases including chronic inflammation and cancer. In this study, we investigated the antioxidant activities and anti-inflammatory effects of the extracts from the herbal teas such as Lonicera japonica Thunberg (L. japonica), Chrysanthemum morifolium Ramat (C. morifolium), Mentha arvensis L. (M. arvensis), and P.rhizoma. Methods : Anti-oxidant activity was evaluated using DPPH radical scavenging assay and $Fe^{2+}$ chelating assay. And DNA cleavage assay was performed to evaluate an anti-oxidative effect. Anti-inflammatory effect was performed using NO generation assay and western blot in LPS-stimulated RAW264.7 cell line. Results : L. japonica scavenged DPPH radical by 9.8% at 12.5 ${\mu}g/ml$, 24.8% at 25 ${\mu}g/ml$, 34.3% at 50 ${\mu}g/ml$, 61.1% at 100 ${\mu}g/ml$ and 75.8% at 200 ${\mu}g/ml$, respectively. In addition, C. morifolium and M. arvensis removed DPPH radical by 15.6% and 10.4% at 12.5 ${\mu}g/ml$, 34.8% and 22.8% at 25 ${\mu}g/ml$, 66.9% and 43.3% at 50 ${\mu}g/ml$, 87.4% and 69.1% at 100 ${\mu}g/ml$, and 92.1% and 73.2% at 200 ${\mu}g/ml$, respectively. However, P. rhizoma did not affect on DPPH radical scavenging. The $Fe^{2+}$ chelating activity was highest in L. japonica, but lowest in P. rhizoma among the herbal teas. In addition, the extracts from L. japonica, C. morifolium and M. arvensis inhibited oxidative DNA damage via its anti-oxidant activity. In anti-inflammatory effect, the extracts from C. morifolium inhibited NO production. In addition, it suppressed the $NF-{\kappa}B$ signaling pathway in LPS-stimulated RAW 264.7 cells. Conclusions : Together, this study indicates that L. japonica, M. arvensis and C. morifolium possess the protective effect against the oxidative DNA damage. Furthermore, C. morifolium exerts an anti-inflammatory effect.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.411-418
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• 2013

The black pine bast scale, Matsucoccus thunbergianae is one of the most serious insect pest in Japanese black pine, Pinus thunbergii forest in Korea. Insecticidal activity of 10 folds hot water extracts from 7 herbal plants (Atractylodes lancea, Eugenia caryophyllata, Lonicera japonica, Melia azedarach, Quisqualis indica, Sophora flavescens and Taraxacum mongolicum) were tested against different stage of M. thunbergianae using spray method both in laboratory and field. Efficacies of herbal extracts were different depending on stage of M. thunbergianae. Q. indica sprayed with hand sprayer produced the highest corrected mortality of 95.7% on intermidiated nymph stage, however, A. lancea produced the highest corrected mortality (51.3%) on pupae of M. thunbergianae in laboratory. Q. indica and A. lancea were highly effective against female M. thunbergianae in laboratory, however, corrected mortality was lower than other stage (40.0%). Efficacy of Q. indica was similar to effective insecticide, fenitrothion 50% EC against M. thunbergianae in field trials. These results indicated that Q. indica could be an environmental friendly control agent of M. thunbergianae.

• Journal of Life Science
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• v.28 no.9
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• pp.1022-1029
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• 2018

This study was carried out to search for the anti-inflammatory activities of ethanol extracts obtained from 5 kinds of oriental medical plant; Pleuropterus multiflorus extract (PME), Acorus calamus L. extract (ACE), Lithospermum erythrorhizon Siebold & Zucc. extract (LEE), Xanthium strumarium L. extract (XSE), Lonicera japonica extract (LJE), which have traditionally been used as a drug in oriental medical plants in Korea. XSE showed cytotoxicity at 100, $200{\mu}g/ml$ concentration in RAW264.7 cells (p<0.05) and ACE showed cytotoxicity at $200{\mu}g/ml$ concentration in RAW264.7 cells (p<0.05). But other oriental medical plants did not showed cytotoxicity was observed in RAW264.7 cells below $200{\mu}g/ml$ concentration. These extracts at non-toxic concentrations showed anti-inflammatory effects. PME, ACE, XSE and LJE showed a concentration-dependent inhibitory effect on NO production and $PGE_2$ production in LPS-induced RAW264.7 cells. In particular, XSE showed the highest NO production inhibition ($52.9{\mu}g/ml$, $IC_{50}$) as well as the highest $PGE_2$ production inhibition at $50{\mu}g/ml$ (73.6%). ACE and LEE showed cell proliferation effects on HaCaT keratinocyte cells. Especially, LEE showed 21.1, 53.5 and 99.6% proliferative activity by incubation for 1, 3, 5days at $100{\mu}g/ml$ concentration. ACE also showed 11.2, 26.0% proliferative activity for 1day and 3days at $10{\mu}g/ml$ concentration. As a result of this study, ethanol extracts obtained from 5 kinds of oriental medical plant showed anti-inflammatory activity and HaCaT cell regeneration effect.

• Korean Journal of Medicinal Crop Science
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• v.15 no.6
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• pp.371-390
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• 2007

Arthropod pests were surveyed from 132 herb species of 121 genera in 50 families of 32 orders at herb garden of Sancheong-gun Agricultural Development Technology Center in Sancheong, Gyeongsangnamdo province from October, 2005 to November, 2006. Ninety eight arthropod pests of 86 genera in 44 families of 9 orders were collected. Although less than 5 arthropod pests were collected from each herb, more than 5 arthropod pests were occurred on Hibiscus mutabililis, Peucedanum japonicum, Aralia cordata, Fraxinus rhynchophylla, Angelica decursiva, Rhaponticum uniflorum, Lonicera japonica, Betula platyphylla and Agrimonia pilosa. Out of collected pests, 98.5% of them damaged leaves of medicinal plants. The highest number of arthropod pests was recorded in May representing 36 species of 32 genera in 20 families of 6 orders. Most of them were collected from less than 5 medicinal plants. However, Atractomorpha lata, Dolycoris baccarum, Myzus persicae, and Nysius plebejus occurred on 42., 22, 20, and 15 medicinal plants, respectively. Atractomorpha lata and Myzus persicae had broad host ranges and seriously damaged. Aphis gossypii was dominant insect pest in May compared with Dolycoris baccarum in June. Atractomorpha lata in August and September, and Myzus persicae in October.

• Korean Journal of Medicinal Crop Science
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• v.25 no.5
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• pp.322-327
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• 2017

Background: Cynaroside is a flavone, a flavonoid-like compound, known by different names (luteoloside and cinaroside). It is commonly found in Lonicera japonica Thunb., Chrysanthemum moriflium, and Angelica keiskei. The process of cell death has been classified as necrosis and apoptosis. Necrosis refers to unregulated cell death induced by a chemotherapeutic agent. Doxorubicin is an anthracycline anti-cancer drug used to treat acute leukemia, cancer, and lymphoma. However, it induces nephrotoxicity including tubular damage. Therefore, we investigated the protective effect of cynaroside against doxorubicin-induced necrosis in HK-2 cells. Methods and Results: To confirm the beneficial effect of cynaroside on doxorubicin-induced necrosis, HK-2 cells, a human proximal tubule epithelial cell line were treated with $10{\mu}M$ doxorubicin and $80{\mu}M$ cynaroside. Doxorubicin treatment resulted in increased DNA fragmentation, caspase-3 activity and mitochondria hyperactivation during cell necrosis. However, pretreatment with $80{\mu}M$ cynaroside attenuated DNA fragmentation, caspase-3 activity and mitochondria hyperactivation induced by $10{\mu}M$ doxorubicin in HK-2 cells. Conclusions: These results indicated that pretreatment with cynaroside ameliorated doxorubicin-induced necrosis in HK-2 cells. Therefore, cynaroside be used as a therapeutic agent for improving doxorubicin-induced nephrotoxicity. However, further studies are required to evaluated the toxicity of cynaroside treatment in animals and to determine its protective effect against doxorubicin-induced nephrotoxicity in an animal model.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.50 no.3
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• pp.239-250
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• 2024

In this study, we investigated the skin safety, antioxidant, moisturizing, and anti-inflammatory effects of a composite extract of Prunus mume flower, Lonicera japonica flower, Chrysanthemum indicum flower, and Phyllostachys bambusoides (Royal TheraTea Guard^TM, RTG), which has been used as an internal medicine in the royal medical treatment for preventing skin damage caused by external environmental factors. In a cytotoxicity test on HaCaT cells, RTG complex extract showed no change in cell viability at concentrations of 0.125, 0.25, 0.5, and 1%, and a skin irritation index of 0.00 in a human patch test, confirming that it is non-irritating to the skin. The antioxidant effects was confirmed by the presence of 497.83 ㎍ GAE/g of polyphenols and increased DPPH radical scavenging activity, with a significant increase in catalase activity in the stratum corneum, showing potential as an antioxidant that protects cells from oxidative stress. The anti-inflammatory effects was observed through reduced erythema in skin stimulated by tape stripping and treated with fine dust in keratinocytes. The moisturizing effects was shown by increased expression of hyaluronan synthase (HAS)2, 3 and keratin1 in keratinocytes treated with fine dust compared to the control group, as well as increased skin moisture content and decreased skin roughness. These results suggest RTG can be used as a cosmetic ingredient to prevent skin damage caused by external environmental factors.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.2
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• pp.28-39
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• 2008

This study was conducted to evaluate the rehabilitation measures and select appropriate tree species and rehabilitation methods for rehabilitating the rock desert areas in Guizhou, China. The study was carried out during the periods from 2003 to 2006 and results are as follows. Afforestation site is classified as a dolomite sandstone hilly country, a halfstony hilly country, a karst physiognomy, a rock desertification farmland and an latent rock desertification farmland by soil type and rock desertification status. Total afforestation area of Xuiwen is 1,300ha and afforestation area of a dolomite sandstone hilly country, a halfstony hilly country, a karst physiognomy, a rock desertification farmland and an latent rock desertification farmland are respectively 104.0ha (8.0%), 146.6ha (11.3%), 200.5ha (15.4%), 705.7ha (54.3%) and 143.2ha (11.0%). Planted tree species were selected by five site types. Robinia pseudoacacia, Chamaecyparis obtusa, Thuja orientalis, Cupressus duclouxiana and Juniperus chinensis L. var. chinensis were planted in a dolomite sandstone hilly country and Choerospondias axillaris and Robinia pseudoacacia were planted in a halfstony hilly country. Cupressus duclouxiana, Zanthoxylum bungeanum, Lonicera fulvotomentosa, Ilex kudincila, Quercus aliena, Quercus variabilis, Choerospondias axillaris, Koelreuteria bipinnata, Cryptomeria japonica, Cinnamomum camphora and Cinnamomum bodinieri were planted in a karst physiognomy. Cupressus duclouxiana, Toona sinensis, Robinia pseudoacacia, Lonicera fulvotomentosa, Eucommia ulmoides, Ilex kudincha. Zanthoxylum bungeanum and Magnolia officinalis were planted in a rock desertification farmland. Lonicera fulvotomentosa, Juglans sinensis, Pyrus pyrifolia, Choerospondias axillaris, Prunus salicina and Diospyros Kaki was planted in an latent rock desertification farm land. Robinia pseudoacacia showed high survival rate in 2004 and 2005, but Zanthoxylum bungeanum and Cinnamomum camphora showed slightly low survival rate in 2004. Survival rate of planting species in 2005 was over 80% because some species which showed low survival in 2004 were changed with other species and a proper afforestation method was applied. Growth rate of tree species was evaluated by height and diameter growth rate. Cupressus duclouxiana showed a highest height growth rate in 2004. Toona sil1ensis, Choerospondias axillaris, Koelreuteria bipinnata, and Cinnamomum camphora showed almost 100% height growth rate in 2005, although they showed a negative height growth rate in 2004. Robinia pseudoacacia showed a good growth rate in the rock desertification farmland and the halfstony hilly country so it could give a commercial benefit like honey as well as an afforestation effect.