The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-oxidant and anti-inflammatory activities of the various kinds of herbal tea

  • Lee, Jin Wook (Department of Bioresource Sciences, Andong National University) ;
  • Eo, Hyun Ji (Department of Bioresource Sciences, Andong National University) ;
  • Park, Gwang Hun (Department of Bioresource Sciences, Andong National University) ;
  • Song, Hun Min (Department of Bioresource Sciences, Andong National University) ;
  • Woo, So Hee (Department of Bioresource Sciences, Andong National University) ;
  • Kim, Mi Kyoung (Department of Bioresource Sciences, Andong National University) ;
  • Eom, Jung Hye (Department of Medicinal Plant Resources, Andong National University) ;
  • Lee, Man Hyo (Gyeongbuk Institute for Bio-industry) ;
  • Lee, Jeong Rak (Gyeongbuk Institute for Bio-industry) ;
  • Koo, Jin Suk (Department of Bioresource Sciences, Andong National University) ;
  • Jeong, Jin Boo (Department of Bioresource Sciences, Andong National University)
  • Received : 2014.02.21
  • Accepted : 2014.03.13
  • Published : 2014.03.30
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Abstract

Objectives : Reactive oxygen species (ROS) are involved in a wide spectrum of diseases including chronic inflammation and cancer. In this study, we investigated the antioxidant activities and anti-inflammatory effects of the extracts from the herbal teas such as Lonicera japonica Thunberg (L. japonica), Chrysanthemum morifolium Ramat (C. morifolium), Mentha arvensis L. (M. arvensis), and P.rhizoma. Methods : Anti-oxidant activity was evaluated using DPPH radical scavenging assay and $Fe^{2+}$ chelating assay. And DNA cleavage assay was performed to evaluate an anti-oxidative effect. Anti-inflammatory effect was performed using NO generation assay and western blot in LPS-stimulated RAW264.7 cell line. Results : L. japonica scavenged DPPH radical by 9.8% at 12.5 ${\mu}g/ml$, 24.8% at 25 ${\mu}g/ml$, 34.3% at 50 ${\mu}g/ml$, 61.1% at 100 ${\mu}g/ml$ and 75.8% at 200 ${\mu}g/ml$, respectively. In addition, C. morifolium and M. arvensis removed DPPH radical by 15.6% and 10.4% at 12.5 ${\mu}g/ml$, 34.8% and 22.8% at 25 ${\mu}g/ml$, 66.9% and 43.3% at 50 ${\mu}g/ml$, 87.4% and 69.1% at 100 ${\mu}g/ml$, and 92.1% and 73.2% at 200 ${\mu}g/ml$, respectively. However, P. rhizoma did not affect on DPPH radical scavenging. The $Fe^{2+}$ chelating activity was highest in L. japonica, but lowest in P. rhizoma among the herbal teas. In addition, the extracts from L. japonica, C. morifolium and M. arvensis inhibited oxidative DNA damage via its anti-oxidant activity. In anti-inflammatory effect, the extracts from C. morifolium inhibited NO production. In addition, it suppressed the $NF-{\kappa}B$ signaling pathway in LPS-stimulated RAW 264.7 cells. Conclusions : Together, this study indicates that L. japonica, M. arvensis and C. morifolium possess the protective effect against the oxidative DNA damage. Furthermore, C. morifolium exerts an anti-inflammatory effect.

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References

  1. Barnham KJ, Masters CL, Bush AI. Neurodegenerative diseases and oxidative stress. Nat Rev. 2004 ; 3 : 205-14.
  2. Yanai N, Shiotani S, Hagiwara S, Nabetani H, Nakajima M. Antioxidant combination inhibits reactive oxygen species mediated damage. Biosci Biotechnol Biochem. 2008 ; 72 : 3100-6. https://doi.org/10.1271/bbb.80159
  3. Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol. 2001 ; 54 : 176-86. https://doi.org/10.1136/jcp.54.3.176
  4. Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agri Food Chem. 2005 ; 53 : 1841-56. https://doi.org/10.1021/jf030723c
  5. Chan EWC, Lim YY, Chong KL, Tan JBL, Wong SK. Antioxidant properties of tropical and temperate herbal teas. J Food Compos Anal. 2010 ; 23 : 185-9. https://doi.org/10.1016/j.jfca.2009.10.002
  6. Naik E, Dixit VM. Mitochondrial reactive oxygen species drive proinflammatory cytokine production. J Exp Med. 2011 ; 208 : 417-20. https://doi.org/10.1084/jem.20110367
  7. Geronikaki AA, Gavalas AM. Antioxidants and anti-inflammatory diseases: synthetic and natural antioxidants with anti-inflammatory activity. Com Chem High T Scr 2006 ; 9 : 425-42.
  8. Mukhtar H, Ahmad N. Tea polyphenols: prevention of cancer and optimizing health. Am J Clin Nutr. 2000 ; 71 : 1698-702. https://doi.org/10.1093/ajcn/71.6.1698S
  9. Aoshima H, Hirata S, Ayabe S. Antioxidative and anti-hydrogen peroxide activities of various herbal teas. Food Chem. 2007 ; 103 : 617-22. https://doi.org/10.1016/j.foodchem.2006.08.032
  10. Tsai PJ, Tasai TH, Yu CH, Ho SC. Comparison of NO-scavenging and NO-suppressing activities of different herbal teas with those of green tea. Food Chem. 2007 ; 103 : 181-7. https://doi.org/10.1016/j.foodchem.2006.08.013
  11. Craig WJ. Health-promoting properties of common herbs. Am J Clin Nutr. 1999 ; 70 : 491-9. https://doi.org/10.1093/ajcn/70.3.491s
  12. Si W, Gong J, Tsao R, Kalab M, Yang R, Yin Y. Bioassay-guided purification and identification of antimicrobial components in Chinese green tea extract. J Chromatogr A. 2006 ; 1125 : 204-10. https://doi.org/10.1016/j.chroma.2006.05.061
  13. Hus B, Coupar IM, Ng K. Antioxidant activity of hot water extract from the fruit of the Doum palm, Hyphaene thebaica. Food Chem. 2006 ; 98 : 317-28. https://doi.org/10.1016/j.foodchem.2005.05.077
  14. Jung Y, Surh Y. Oxidative DNA damage and cytotoxicity induced by copper-stimulated redox cycling of salsolinol, a neurotoxic tetrahydroisoquinoline alkaloid. Free Radical Biol Med. 2001 ; 30 : 1407-17. https://doi.org/10.1016/S0891-5849(01)00548-2
  15. Kong AN, Yu R, Hebbar V, Chen C, Owuor E, Hu R, Ee R, Mandlekar S. Signal transduction events elicited by cancer prevention compounds. Mutat Res. 2001 ; 480-481 : 231-41. https://doi.org/10.1016/S0027-5107(01)00182-8
  16. Durackova Z. Some current insights into oxidative stress. Physiol Res. 2010 ; 59 : 459-69.
  17. Fang J, Seki T, Maeda H. Therapeutic strategies by modulating oxygen stress in cancer and inflammation. Adv Drug Deliv Rev. 2009 ; 61 : 290-302. https://doi.org/10.1016/j.addr.2009.02.005
  18. Khandrika L, Kumar B, Koul S, Maroni P, Koul HK. Oxidative stress in prostate cancer. Cancer Lett. 2009 ; 282 : 125-36. https://doi.org/10.1016/j.canlet.2008.12.011
  19. Niki E, Noguchi N. Evaluation of antioxidant capacity. What capacity is being measured by which method? IUBMB Life. 2000 ; 50 : 323-9 https://doi.org/10.1080/15216540051081119
  20. Reddy BD, Reddanna P. Chebulagic acid (CA) attenuates LPS-induced inflammation by suppressing NF-${\kappa}B$ and MAPK activation in RAW 264.7 macrophages. Biochem Biophys Res Commun. 2009 ; 381 : 112-7. https://doi.org/10.1016/j.bbrc.2009.02.022
  21. Mankan AK, Lawless MW, Gray SG, Kelleher D, McManus R. NF-kappaB regulation: the nuclear response. J Cell Mol Med. 2009 ; 13 : 631-43. https://doi.org/10.1111/j.1582-4934.2009.00632.x
  22. Sarkar FH, Li Y, Wang Z, Kong D. NF-kappaB signaling pathway and its therapeutic implications in human diseases. Int Rev Immunol. 2008 ; 27 : 293-319. https://doi.org/10.1080/08830180802276179