• 한국토양비료학회지
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• 제49권5호
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• pp.608-613
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• 2016

Arbuscular mycorrhizal fungi (AMF) spore propagation and long term maintenance is still a complicated technique for farmers. The use of AMF for their ability to promote plant growth and protect plants against pathogen attack and environmental stresses demands AMF propagation for large scale application. This study aimed to propagate AMF spores by trap culture technique and assess their ability to propagate with different host plants in a continuous plant cycle. Mycorrhizal inoculation by trap culture in maize resulted in longer shoots and roots than sudangrass plants. Increase in dry weight with higher percentage also was observed for maize plants. After first and second plant cycle, maize plants had the higher percentage of mycorrhizal response in terms of colonization and arbuscules than sudangrass. Maximum in spore count also achieved in the pots of maize plants. The results show that maize plant is more suitable host plant for AMF spore propagation and trap culture technique can be used effectively to maintain the AMF culture for long time.

• Clinical and Experimental Pediatrics
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• 제62권12호
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• pp.433-437
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• 2019

Acute pyelonephritis (APN) should be detected and treated as soon as possible to reduce the risk of the development of acquired renal scarring. However, in the medical field, urine culture results are not available or considered when the prompt discrimination of APN is necessary and empirical treatment is started. Furthermore, urine culture cannot discriminate APN among children with febrile urinary tract infection (UTI) (pyelitis, lower UTI with other fever focus). Therefore, the usefulness of urine culture for diagnostic purposes is small and the sampling procedure is invasive. Congenital hypoplastic kidney is the most common cause of chronic kidney injury in children. Thus, it is desirable that a main target be detected as early as possible when imaging studies are performed in children with APN. However, if APN does not recur, no medical or surgical treatment or imaging studies would be needed because the acquired renal scar would not progress further. Therefore, the long-term prognosis of APN in young children, particularly infants, depends on the number of recurrent APN, not other febrile UTI. New methods that enable prompt, practical, and comfortable APN diagnosis in children are needed as alternatives to urinary catheterization for urine culture sampling.

• 의학교육논단
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• 제17권3호
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• pp.105-109
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• 2015

The purpose of this study is to examine the concept and importance of the hidden curriculum, which has an influence on the learning, culture, and identity formation of medical students, and to examine the student culture related to the hidden curriculum. The hidden curriculum can be defined from various perspectives. However, these definitions commonly include the concept of the whole experience students gain from school life in implicit ways, even though the school does not intend it. The hidden curriculum is related to non-cognitive areas and the culture formation of students in various way, including positive and negative content, and is important since once this curriculum is formed, it has a long-term impact. Therefore, it is necessary to consider not only the formal curriculum but also the hidden curriculum in order to apprehend the overall educational outcome of medical school. For this purpose, schools need to not only support studies on the hidden curriculum but also to endeavor to provide faculty and staff with educational and administrative support so that they can understand the hidden curriculum and be equipped as a role model. Furthermore, medical students need to endeavor to form a positive student culture in order to establish an appropriate identity as a doctor in the future.

• 한국조명전기설비학회:학술대회논문집
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• 한국조명전기설비학회 2002년도 학술대회논문집
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• pp.233-237
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• 2002

In consideration for evaluating urban lighting plan as culture of a society, preparing lighting plan while designing urban plan is a fundamental process. This study proposes a methodology, maintaining and embossing urban throughout preparing a long-term urban lighting plan considering overall urban space as well as improving competitive power for lighting to take an important role in total environmental design project.

• 한국생물환경조절학회:학술대회논문집
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• 한국생물환경조절학회 1998년도 정기총회 및 학술논문 발표요지
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• pp.116-121
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• 1998

단고추는 국내에서는 1934년에 "복견감장" 이라는 품종을 도입한 것이 최초로 알려져 있으며 그후 영리를 목적으로 본격적인 농가재배가 이루어진 것은 80년대부터이다$^{3)}$ . 그후로 지속적으로 재배면적이 증가되어 단고추의 재배면적은 ＇97년 현재100㏊ 정도 재배되고 있고 식생활의 변화와 더불어 그 소요가 크게 증가되어 면적도 증가 추세에 있는 작물중의 하나다. (중략) 하나다. (중략)

• 한국수정란이식학회지
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• 제29권2호
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• pp.133-139
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• 2014

$K^+$ channels are involved in the regulation of a variety of physiological functions, including proliferation, apoptosis and differentiation, in mammalian cells. Our previous study demonstrated that the blockage of $K^+$ channels inhibits mouse early embryonic development. This study was designed to identify the effect of $K^+$ channels during bovine embryonic development. $K^+$ channel blockers (tetraethylammonium (TEA), $BaCl_2$, quinine, ruthenium red and fluoxetine) were added to the culture medium during in vitro fertilization (IVF) for 6 h to first identify the short-term effect of these chemicals. Among $K^+$ channel blockers, fluoxetine, which is used as a selective serotonin reuptake inhibitor, significantly increased the blastocyst formation rate by approximately 6% when compared to control. During the in vitro maturation (IVM) of immature oocytes and the in vitro culture (IVC) of embryos, the oocytes and embryos were exposed to fluoxetine for either a short-term (6 h) or a long-term (24 h) to compare the embryonic development in response to exposure time. The 6 h exposure to fluoxetine during IVM did not affect the blastocyst formation rate, but the rate of blastocyst formation was reduced after the 24 h exposure. On the other hand, embryonic development increased approximately 10% in both groups of embryos exposed to fluoxetine for 6 and 24 h during IVC. Taken together, fluoxetine treatment during IVF and IVC, but not IVM, enhances bovine embryonic development. These results suggest that fluoxetine-modulated signals in oocytes and embryos could be an important factor towards enhancing bovine embryonic development.

• Journal of Animal Science and Technology
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• 제54권6호
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• pp.427-433
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• 2012

The present study was performed to investigate long-term effects of the low-crude protein and lysine (low CP/lys) diet and a yeast culture supplemented to the low CP/lys diet on growth and carcass characteristics in growing-finishing pigs. Forty-five gilts and 45 barrows weighing approximately 25 kg born to Yorkshire ${\times}$ Landrace dams and Duroc sires were allocated to nine pens, with five gilts and five barrows assigned per pen. Every three pens received CP/lys-rich grower and finisher diets (control), low CP/lys grower and finisher (basal), or the low CP/lys grower and finisher supplemented (2%) with a yeast culture providing $3.2{\times}10^8$ Saccharomyces cerevisiae cells/kg diet (yeast) for 31 and 79 days, respectively. The ADG was less in the low CP/lys (basal + yeast) group than in the control group (P<0.01) during both grower (0.59 vs. 0.70 kg) and finisher (0.75 vs. 0.89 kg) phases. However, marbling score was greater (P<0.05) in the low CP/lys group vs. control (3.86 vs. 3.12) at slaughter, which resulted in a twice percentage of the $1^+$-quality grade carcasses in the former compared with that for the latter. Supplementation of the yeast culture to the basal diet caused a decrease in ADG during the grower phase (P<0.01), but not during the finisher phase (0.64 vs. 0.53 kg and 0.73 vs. 0.77 kg for the basal vs. yeast group during the grower and finisher phases, respectively), without influencing the marbling score or percentage of the $1^+$-quality grade carcasses. In conclusion, results indicate that long-term provision of the low CP/lys diet has a beneficial effect on marbling and carcass quality grade as well as a negative effect on weight gain and that the yeast culture supplemented to the low CP/lys diet has no beneficial effect on weight gain or carcass quality.

• Journal of Periodontal and Implant Science
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• 제26권1호
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• pp.216-229
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• 1996

Interferon(IFN) is a sort of glycoproteins that are produced by activated lymphocyte, monocyte and fibroblast. IFN has anti-viral effects, immuno-defensive mechanism and regulating properties to the several kinds of cells that includes affect on the bone formation and resorption. The effect of IFN on the osteoclast & other tissue cells has been studied in a number of researchers with the limited reports on the osteoblast. The purpose of this study was to evaluate the effects of IFN on the osteoblastic function. The MC3T3/El cell(Mouse osteoblast) was incubated in ${\alpha}-minimum$ essential medium containing 10% FBS. To detect the cytotoxic effect of $IFN-{\gamma}$ on osteoblast, the cells were cultured in 96-well plate to which $IFN-{\gamma}$ of various concentrations were added for 2 days. After staining with trypan blue, total cells and living cells were counted under microscope. To determine the activity of alkaline phosphataset(ALP), various concentrations of $IFN-{\gamma}$ were treated to culture medium, and biochemical assay was performed. $IFN-{\gamma}$ and $IFN-{\gamma}$ plus cycloheximide were added to culture medium separately and then ALP activity were determined. To detect the effect of the $IFN-{\gamma}$ on the bone formation of osteoblast, long-term culture was performed, and calcified nodule formation were observed using von Kossa's staining. After the addition of $IFN-{\gamma}$ with various concentrations to the medium, no cytotoxic effect of $IFN-{\gamma}$ was detected at any concentration. The significant increase in ALP activity of osteoblast were found the concentration of $IFN-{\gamma}$ 500-2500U/ml and the culture time of 24-48 hours respectively. The enhancement of ALP activity by $IFN-{\gamma}$ of osteoblast was decreased significantly by the treatment of cycloheximide. After long-term culture of osteoblast, the nodule formation was found to be increased in number and density by the addition of 500 U/ml $IFN-{\gamma}$. These results suggest that $IFN-{\gamma}$ was affected on the bone formation of osteoblast. Forthemore this kind of study or $IFN-{\gamma}$ to osteoblast will be held continuously.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.88-94
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• 2005

Plant tissue culture studies have been done for the preservation of medicinal plant resources and efficient production of pharmaceutically important secondary metabolites. Micropropagation methods for Cephaelis ipecacuanha have been established and these methods enabled much more efficient propagation of the plants than the conventional methods using seedling or layering. The C. ipecacuanha plants derived from tissue culture grew uniformly in the field and they showed higher alkaloid contents compared to the plants grown from seedlings. Hairy root cultures of C. ipecacuanha and Panax ginseng have been established by infection with Agrobacterium rhizogenes, and the production of important pharmaceuticals by these cultures have been successfully demonstrated. In the case of C. ipecacuanha, the highest alkaloid yields from the hairy roots cultured for 8 weeks were 2.75-fold cephaeline (5.5 mg) and one third emetine (0.7 mg) compared with those from the roots of one-year old plant propagated through shoot-tip culture and cultivated in a greenhouse (2.0 mg cephaeline and 2.0 mg emetine). In the case of P. ginseng, ginsenoside contents in the hairy roots optimally cultured for 4 weeks were much higher than those in the roots of 4-year old field-grown plant. Thus our medicinal plant tissue cultures demonstrate desirable properties. However, they are always exposed to danger of microbial contamination or unexpected trouble of culture facilities. Cryopreservation of plant tissue cultures is a reliable method for long-term preservation. Cryopreservation studies on these cultures are also presented.

• BMB Reports
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• 제36권6호
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• pp.565-571
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• 2003

The locomotor responses of human peripheral blood neutrophils and lymphocytes were measured by the change from spherical to polarized shapes in the presence of endotoxins (lipopolysaccharide, LPS) of enteric pathogens: S. dysenteriae type 1, V. cholerae Inaba 569B, S. typhimurium, and K. pneumoniae. We reported earlier that these endotoxins are chemotactic factors for the neutrophils since they stimulated cell polarization within a few minutes of incubation. Endotoxins had an inhibitory effect upon neutrophil phagocytosis of opsonized yeast and the cells engulfed fewer yeasts. Interestingly, endotoxins increased neutrophil adhesion to clean glass surfaces, but stimulated the cells to exhibit increased random locomotion (chemokinesis) through cellulose nitrate filters and show an enhanced ability to reduce nitroblue tetrazolium (NBT) dye. Unlike neutrophils, lymphocytes direct from blood do not show polarized morphology towards chemotactic factors but the cells acquire locomotor capacity during 24-72 h culture with mitogens such as phytohemagglutinin (PHA), phorbol myristate acetate or concanavalin A. Stimulation of blood lymphocytes with endotoxins did not induce cell polarization in short-term but long-term culture resulted in an increase in the proportion of polarized cells that acquired locomotor morphologies. The majority of these cells were identified as esterase negative B-lymphocytes that migrated through filters. Despite the optimum time of incubation for each of these cell types being different, we found that lymphocytes respond to much lower concentrations of endotoxins than the neutrophils. These findings suggest that endotoxins of enteric pathogens modulate the functions of human blood neutrophils and lymphocytes.