• 한국방사선학회논문지
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• 제7권1호
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• pp.9-15
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• 2013

본 연구는 전산화단층촬영에서 간 질환의 자동 인식으로 질감특징분석(texture feature analysis. TFA) 알고리즘을 제안하고자 하였으며, 간세포암(Hepatocellular carcinoma. HCC)에 대한 컴퓨터보조진단(computer-aided diagnosis. CAD) 시스템을 설계하고, 제안하는 각 알고리즘의 성능을 평가하고자 하였다. HCC 영상에서 분석영역($40{\times}40$ 픽셀)을 설정하고 각 부분영상에 통계적 특징을 이용한 6가지 TFA 파라메터(평균 밝기, 평균 대조도, 평탄도, 왜곡도, 균일도, 엔트로피)비교하여 간세포암 인식률(recognition rate)을 구하였다. 결과적으로 TFA는 간세포암 인식률을 나타내는 척도로 유의함을 알 수 있었으며 6가지 파라메터에서 균일도가 가장 인식률이 높았으며 평균 대조도, 평탄도, 왜곡도가 비교적 높았고 평균 밝기와 엔트로피는 상대적으로 낮은 인식률을 나타내었다. 이와 관련하여 높은 인식률을 보인 알고리즘(최대 97.14%, 최소 82.86%)을 간세포암 영상의 병변을 판별하여 임상의 조기 진단을 보조하여 치료를 시행한다면 진단의 효율성이 높아 질 것으로 판단되었으며, 향후 효율적이고 정량적인 분석을 추가함으로써 질병인식의 일반화에 대한 기준 연구가 필요 할 것으로 사료되었다.

• Journal of Acupuncture Research
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• 제33권2호
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• pp.21-34
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• 2016

Objectives : The aim of the present study is to examine the effect and mechanism of Erycibae Caulis and Corydalis Tuber Pharmacopuncture (ECP) on a mouse model with collagen induced rheumatoid arthritis (CIA). Methods : We evaluated the Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Creatinine, and the Blood urea nitrogen (BUN) of serum to examine the safety of this study. In vivo, we compared the results of the non-treated group, the normal saline pharmacopuncture treated control group, the indomethacin treated group and the ECP group. We evaluated rheumatoid arthritis manifestation and the Rheumatoid Arthritis Index (AI). Also, immune cells in blood affected by ECP were evaluated by calculating the level of white blood cells (WBC), neutrophil, lympocytes and monocytes. Next, the level of Immunoglobulin M (IgM), Immunoglobulin G (IgG), Interleukin (IL)-$1{\beta}$, IL-6, IL-17, Tumor Necrosis Factor (TNF)-${\alpha}$ and Granulocyte-macrophage Stimulating Factor (GM-CSF)in serum were measured. We examined the imaging of cartilage degeneration using micro CT-arthrography of the hind paw. Additionally, we examined the effects of reducing bone volume (BV) ratio and bone surface/bone volume (BS/BV) ratio with 3D Micro-CT. Finally, we did a histopathologic examination analysis. Results : The absence of liver and kidney toxicity was evident. In vivo, edema of the joints of the ECP group decreased greatly in macroscopic observation. AI measurement of the ECP group also decreased significantly compared to the control group. The level of WBC, neutrophil, lympocytes, and monocytes in the blood decreased but there was no statistical significance of this data. IgM of the ECP group decreased significantly compared to the control group. IL-$1{\beta}$, IL-6, TNF-${\alpha}$, and GM-CSF production of the ECP group decreased significantly compared to the control group. As a result of examining joint condition with 3D micro CT, deformation and destruction of the joint was shown to have decreased. Bone density of ECP group increased at a statistically significant level compared to the control group. Degree of joint inflammation of ECP group decreased significantly compared to the control group. After H&E and M-T staining, infiltration of immune cells, subsidence of the cartilage, damage to the synovial cells and joint erosion decreased. Conclusion : This study showed that ECP hindered the process of rheumatoid arthritis and protected joints and cartilage.

• 혜화의학회지
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• 제24권2호
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• pp.35-57
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• 2016

Objectives : The purpose of this study is to prove the effect and mechanism of Gamikyejakjimogawusul-tang(GKHA) herbal acupuncture on induced rheumatoid arthritis model of DBA/1 mice. Methods : We check effect of GKHA extract on the AST, ALT, Creatinine, BUN of serum and cell viability of GK extract in RAW 264.7 cells to test the stability of this study. In vitro, we measure total phenol contents, total flavonoid contents, DPPH free radical scavenging activity, ABTS cation radical scavenging activity of Gamikyejakjimogawusul-tang, effect of GK extract on ROS(Reactive Ooxygen Species) production to estimate a anti-oxidant capacity, and we also measure effect of GK extract on NO (Nitric Oxid), IL-$1{\beta}$, IL-6, IL-17, IL-21, TNF-${\alpha}$, MCP-1, GM-CSF production in RAW 264.7 cells to estimate a anti-inflammatory efficacy. In vivo, we compare a rheumatoid arthritis manifestation between control and experimental group and estimate a AI. Then we check effect of GKHA on the level of WBC, neutrophil, lympocyte, monocyte in the blood to see the effect of immune cells in blood. In addition we measure effect of GKHA on the level of hs-CRP, IgM, IgG, IL-$1{\beta}$, IL-6, IL-17, IL-21, TNF-${\alpha}$, MCP-1, GM-CSF in serum. We observe effects of GKHA on imaging of cartilage degeneration using micro CT-arthrography in paw hind. And we calculate effects of GKHA that reduced BV ratio, BS/BV ratio using 3D Micro-CT. Lastly we observe effects of GKHA histopathologic examination analysis. Results : 1. The toxicity on liver and kidney was disregardable and the cytotoxicity against RAW 264.7 cells was also disregardable. 1. Total phenol contents and total flavonoid contents in GK extract were in high level. 2. DPPH free radical scavenging activity and ABTS cation radical scavenging activity were increased according to concentration of GK extract 3. ROS production was significantly decreased in GK extract (at 10, $100{\mu}g/ml$). 4. NO, IL-6, TNF-${\alpha}$, MCP-1 production were significantly decreased in GK extract(at 10, $100{\mu}g/ml$). IL-17, GM-CSF production were significantly decreased in GK extract(at 1, 10, $100{\mu}g/ml$). IL-$1{\beta}$, IL-21 production were also decreased but there was no statistical significance. 5. 25x observation after H&E and M-T staining, infiltration of immune cells and subsidence of the cartilage and damage to the synovial cells were decreased. Conclusions : This study showed that GKHA extract had anti-oxidant capacity, anti-inflammatory efficacy. GKHA extract also had inhibiting effect on the process of rheumatoid arthritis and can protect joint and cartilage. So we expect that GKHA extract can be a meaningful treatment to rheumatoid arthritis patients.

• 한국콘텐츠학회논문지
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• 제14권11호
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• pp.558-574
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• 2014

최근 복부비만, 고혈압, 당뇨, 고지혈증과 같은 대사성 증후군에 속하는 질환이 급격히 증가하여 지방간 환자수가 급격히 증가하고 있다. 이를 위해 자기공명영상을 통한 검진이 요구되는데 관심 영역과 관심 영역이 아닌 영역의 intensity 값이 상당히 유사하거나 동일한 경우가 많기 때문에 전문적인 물, 지방 분리 방법을 통해서 분석하는 경우가 많다. 그러나 이러한 분리법은 필드의 불균일성이 큰 고자장으로 갈수록 부정확한 결과가 도출하게 된다. 본 논문은 이러한 기존 분리법의 한계를 극복하고자, 고자장에서도 적용이 가능한 필드 맵 측정 방법을 제안한다. 제안 방법은 기존 IDEAL 시퀀스로 얻어진 에코 영상을 통해 필드 맵을 생성하고, 클러스터링 작업, 그리고 영역성장법 스키마 기반의 least square fit을 통한 필드 맵의 보정, 마지막으로 필드 맵의 homogeneity를 위한 경계선의 보정을 수행하여 최종적으로 물과 지방을 분리한다. 실험결과 IDEAL방법이 평균 61.5%, Yu의 방법이 평균 62.6%, 제안 방법이 평균 86.4%의 지방 검출율로 제안방법이 월등함을 확인하였다. 또한 물 검출율 역시 제안 방법이 평균 98.4%의 물 검출율로 Fat saturation의 평균 88.6% 보다 높은 정확도를 확인 할 수 있었다.

• 대한방사선기술학회지:방사선기술과학
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• 제6권1호
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• pp.103-113
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• 1983

Scintillation cameras을 사용(使用)할때 양질(良質)의 화상(畵像)을 얻기 위하여 camera의 성능(性能), 방사성의약품(放射性醫藥品)의 특성(特性), 그리고 적정(適正)한 촬상조건(撮像條件)을 고려할 필요(必要)가 있다. 특(特)히 RI 상(像)에 대한 촬상조건(撮像條件)은 film의 성질(性質), 상(像)의 크기, 상(像)의 농도(濃度), window, 그리고 콜리메타 전면(前面)으로부터 거리(距離) 등(等)에 따라서 좌우(左右)된다. 이러한 요인(要因)들은 각각 화상(畵像)을 만드는 과정에 영향(影響)을 미치고 있다. 그러므로 상(像)의 표준화(標準化)와 촬상방법(撮像方法)의 기준(基準)을 확립(確立)하기가 매우 어렵다. 고로 이러한 문제(問題)들을 해결(解決)하는 것은 Scintillation camera을 사용(使用)하는 핵의학적(核醫學的) 검사(檢査)에 있어서 매우 중요(重要)하다. 저자(著者)는 이러한 점(點)을 고려하여 일정(一定)한 상(像)의 농도(濃度)을 얻을 수 있는 촬상조건(撮像條件)에 대(對)하여 실험(實驗)을 하였다. 실험(實驗)에 있어서 평행다공형(平行多孔型) 콜리메타를 camera의 head에 부착(附着)시키고 $^{99m}Tc$(3mCi)가 넣어져 있는 liver phantom을 콜리메타의 표면(表面)에 밀착(密着)시켜 다음과 같은 방법(方法)에 의하여 촬상(撮像)하였다. 즉, 일정(一定)한 상(像)의 농도(濃度)을 얻을 수 있는 촬상조건(撮像條件)을 찾아낼 목적(目的)으로 preset time, image size, 콜리메타전면(前面)- phantom간(間) 거리(距離), 그리고 window를 각각(各各) 변화(變化)시키면서 phantom을 촬상(撮像)하였으며, 특(特)히 거리(距離)와 window에 있어서는 cold defects의 검출능(檢出能)도 비교검토(比較檢討)하였다. 실험결과(實驗結果)는 다음과 같다. 1. C type film에 있어서 1.5의 농도(濃度)을 얻기위해서는 7.5의 intensity 에서 preset time은 60초(秒), image size는 1.0, 콜리메타-phantom간거리(間距離)는 0cm 그리고 window는 20%가 요구(要求)되고 있었다. 2. 편면유제(片面乳劑) film의 preset time은 양면유제(兩面乳劑) film보다 짧은 것으로 나타났다. 3. 상(像)의 분해능(分解能)은 image size가 작을수록, 콜리메타-phantom간(間) 거리(距離)가 짧을수록, 그리고 window 폭(幅)이 좁을수록 더욱 더 향상(向上)되고 있었다. 4. Cold defects의 검출능(檢出能)은 image size가 작을수록, 콜리메타-pantom간(間) 거리(距離)가 짧을수록, 그리고 window폭(幅)이 좁을수록 향상(向上)되고 있었다.

• 한국의학물리학회:학술대회논문집
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• 한국의학물리학회 2004년도 제29회 추계학술대회 발표논문집
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• pp.100-103
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• 2004

본 연구에서는 PET/CT시스템에서 CT영상을 이용한 감쇠 보정 시 조영제가 PET영상에 미치는 영향을 관찰하기 위해 팬텀실험과 모의실험을 수행하였다. 다양한 농도로 희석한 조영제를 채운 플라스틱 병을 스티로폼을 이용하여 전신 팬텀 내에 고정시킨 후 투과 영상을 획득하였다. 모의실험을 위해 인체 기관 중 간을 가진 수학적 방출 스캔 맵과 투과 스캔 맵을 각각 만들었다. 간에서 조영제가 비 균일하게 증강된 투과 스캔 맵, 간에 조영제의 증강이 있는 투과스캔 맵과 없는 투과 스캔 맵, 간에 있는 종양에 조영제의 hypo-attenuating이 있는 투과 스캔 맵, 그리고 각각의 다른 조영제의 감쇠 정도를 가지는 간을 구현한 투과 스캔 맵을 각각 만들고 이를 이용하여 방출 영상을 감쇠 보정하였다. 팬텀실험을 통해 조영제의 농도에 따라 감쇠 정도가 달라짐을 확인하였다. 모의실험을 통해 감쇠 보정된 방출 영상이 조영제에 의해 영향을 받고, 또한 인공산물이 발생할 수 있음을 확인하였다. 따라서 조영제와 같은 물리적 인자가 감쇠 보정에 미치는 영향을 충분히 이해하고 정량적 분석 또는 진단 등에 고려하여야 할 것으로 생각한다.

• 한국임상수의학회지
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• 제23권4권
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• pp.388-392
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• 2006

80두의 가토 간에 $1{\sim}2mm^3$ 크기의 VX2 종양세포를 직접 주입하여 단일간암모델을 만들고, 종양이식 후1주, 2주, 3주째에 CT촬영을 실시하여 생성된 종양의 크기와 조영 양상을 관찰하고 조직병리학적검사로 종양의 진위 여부를 확인하였다. 80두 중 66두(82.5%)에서 단일 종양이 만들어졌다. 나머지 12두 중 1두에서 간외종앙의 생성, 그리고 다른 1두에서 육아종성 종괴가 관찰되었으며, 4두는 폐사하였다. 나머지 8두에서는 종양이 생성되지 않았다. 조영 CT에서 관찰된 종양의 크기는 1주, 2주 그리고 3주째에 각각 $7.4{\times}6.3,\;14.2{\times}10.8,\;16.2{\times}12.6cm$로 측정되었다. 모양은 둥글거나 타원형이었으며, 동맥기에서 중앙에 저감쇠와 변연에 조영증강을 보였으며, 문맥기에는 저감쇠를 나타내었다. 종양조직의 이식은 균질한 단일종양모델을 만드는데 매우 유용하고 적합한 방법이며, 조영 CT 촬영술은 간종양 진단 및 다양한 치료방법 적용 후 추적검사에 매우 유용하게 활용될 것으로 판단된다.

• 한국임상수의학회지
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• 제33권4호
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• pp.214-217
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• 2016

A 10-year-old, 24.1 kg, intact female Siberian husky dog (case 1) and 11-year-old, 5.0 kg, intact male Shihtzu dog (case 2) presented with chief complaints of polydipsia, anorexia, vomiting and exercise intolerance (case 1) and stranguria (case 2). Splenic nodule (case 1) and mass (case 2) were identified in these patients through ultrasonographic examination. Laparoscopic splenectomy was conducted for the histopathologic evaluation. In addition, laparoscopic ovariohysterectomy, liver biopsy (case 1) and castration (case 2) were performed for treatment or diagnosis of primary symptoms. Under general anesthesia, 5 mm three-portal access laparoscopic splenectomy was performed using the Sonicision$^{TM}$ equipment. The dogs were rotated onto right lateral recumbency. The spleen was elevated using a fan or goldfinger retractor, which revealed the ventral aspect of the spleen. Resection of vessels was started at the caudal aspect of the spleen using the Sonicision$^{TM}$. The excised spleen was removed from the abdominal cavity using a 12 mm endo-bag via the enlarged instrument portal. There were no post-operative complications in either patient. Histopathologic diagnoses were splenic lymphoid hyperplasia (case 1) and splenic nodular hyperplasia (case 2). Based on our experience, laparoscopic splenectomy is sufficient to replace traditional splenectomy in small animal surgery. The use of the Sonicision$^{TM}$ could be a novel surgical technique for three-portal laparoscopic splenectomy, regardless of patient size.

• Journal of Korean Neurosurgical Society
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• 제50권4호
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• pp.281-287
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• 2011

Objective : Pituitary apoplexy is life-threatening clinical syndrome caused by the rapid enlargement of a pituitary tumor due to hemorrhage and/or infarction. The pathogenesis of pituitary apoplexy is not completely understood. We analyzed the magnetic resonance imaging (MRI) of pituitary tumors and subsequently correlated the radiological findings with the clinical presentation. Additionally, immunohistochemistry was also performed to determine whether certain biomarkers are related to radiological apoplexy. Methods : Thirty-four cases of pituitary adenoma were enrolled for retrospective analysis. In this study, the radiological apoplexy was defined as cases where hemorrhage, infarction or cysts were identified on MRI. Acute clinical presentation was defined as the presence of any of the following symptoms: severe sudden onset headache, decreased visual acuity and/or visual field deficit, and acute mental status changes. Angiogenesis was quantified by immunohistochemical expression of fetal liver kinase 1 (Flk-1), neuropilin (NRP) and vascular endothelial growth factor (VEGF) expression, while microvascular density (MVD) was assessed using Endoglin and CD31. Results : Clinically, fourteen patients presented with acute symptoms and 20 for mild or none clinical symptoms. Radiologically, fifteen patients met the criteria for radiological apoplexy. Of the fifteen patients with radiologic apoplexy, 9 patients presented acute symptoms whereas of the 19 patient without radiologic apoplexy, 5 patients presented acute symptoms. Of the five biomarkers tracked, only VEGF was found to be positively correlated with both radiological and nonradiological apoplexy. Conclusion : While pituitary apoplexy is currently defined in cases where clinical symptoms can be histologically confirmed, we contend that cases of radiologically identified pituitary hemorrhages that present with mild or no symptoms should be designated subacute or subclinical apoplexy. VEGF is believed to have a positive correlation with pituitary hemorrhage. Considering the high rate of symptomatic or asymptomatic pituitary tumor hemorrhage, additional studies are needed to detect predictors of the pituitary hemorrhage.

• Advances in pediatric surgery
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• 제15권1호
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• pp.11-17
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• 2009

Choledochal cyst is a congenital dilatation of the bile duct. Intrahepatic bile duct dilatation of type IVa by Todani's classification at the time of diagnosis resolved spontaneously after cyst excision and hepaticojejunostomy in many cases. It should be distinguished from the true cystic dilatation of the intrahepatic ducts, which tends to persist, albeit after some regression. We therefore studied postoperative intrahepatic duct dilatation changes in choledochal cyst. A total of seventy-six choledochal cysts were managed at the Division of Pediatric Surgery, Department of Surgery, Samsung Medical Center from May 1995 to December 2005. The ratio of males to females was 1:2.8. Preoperative radiologic diagnosis by Todani's classification was Type I (n=52, 68.4 %), II (n=1, 1.3 %), IVa (n=23, 30.3 %). Among fifty-five patients with intrahepatic bile duct dilatation we were able to follow up forty-eight by ultrasonography. Twenty-two patients were type IVa, and twenty-six patients were type I and showed intrahepatic duct dilatation. Mean follow-up duration was 35.3 months (9~105 months). Complete regression of dilated intrahepatic duct was observed in fifteen patients of type IVa and twenty-four patients of type I. Incomplete regression of dilated intrahepatic duct was observed in six patients in type IVa and two patients in type I. Only one patient in type IVa showed no change in ductal dilatation during a follow-up period of 15 months. We conclude that true type IVa is much less frequent than what was diagnosed preoperatively by imaging study. Therefore in type IVa patients who are diagnosed preoperatively the decision to perform liver resection should be carefully considered. Postoperative long term follow up of choledochal cyst with intrahepatic bile duct dilation is needed.