• Nutritional Sciences
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• 제5권2호
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• pp.53-59
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• 2002

This study was conducted to examine the effects of dietary sources of fatty acids and protein on serum protein profiles, hepatic functional enzyme activities, mammary tumor incidence and tumor weight in 7, 12-dimethylbenz($\alpha$)anthracene (DMBA)-treated rats. The sources of dietary fatty acids were 18n6 (rich in linoleic acid), 18n3 (rich in linolenic acid) and 22n3 (rich in DHA) : sources of dietary protein were casein (C) and soy protein isolate (S). mammary tumors (MTs) were chemically induced by DMBA (9 mg/100 g body weight) which was gastrically intubated at 7 weeks of age. Each experimental diet was given for the following 25 weeks. Casein-fed rats (group C) exhibited significantly higher levels of weight gain and FER (food efficiency ratio) than did group S. Group C showed higher levels of serum protein and globulin, and higher albumin/globulin (A/G) ratios than group S. Liver functional enzyme activities (GOT, GPT, ALP, LDH, $\gamma$-GT) and LDH/GOT ratios were not influenced by dietary protein. GPT activity was lower in the group given 18n3, and ALP activity was lower in the group given 18n6. The incidence and total number of MTs appeared to be lower in the group given 22n3 than in the group given 18n3 or 18n6, even though the average weight of MTs was highest in the group given 22n3, The average weight of MTs was higher in the C group than in the S group. MT incidence had a positive correlation with LDH activity and LDH/GOT ratio. The average weight of MTs had a negative correlation with serum albumin levels and A/G ratios, and a positive correlation with ALP activity. This research suggests that the measurement of serum protein profiles and liver functional enzyme activities may be utilized to monitor the development of mammary tumors.

• 대한방사선기술학회지:방사선기술과학
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• 제16권2호
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• pp.87-94
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• 1993

In order to investigate radiation effects on the liver, functional changes of liver were analyzed after irradiation. Doses of 10 Gy, 15 Gy and 20 Gy were exposed partially to the liver of male rats(Sprague-Dawley) with X-ray(4MV linear accelerator) at room temperature. On 1, 2, 4 and 8 weeks after irradiation, liver tissues and sera of the animals were compared with those of unirradiated animal by liver function tests. Enzyme activities in sera such as alanine aminotransferase, aspartate aminotransferase, malondialdehyde. The content of malondialdehyde in the activities of many enzymes including alanine aminotransferase, aspartate aminotransferase in sera were increased slightly with increasing exposure dose in all experiments and the activities of these enzymes increased markedly in 20 Gy irradiated groups. From these above results, functional changes of the liver were induced in all irradiated groups. Damaged liver was recovered along with time collapse after irradiation to the doses of 10 Gy and 15 Gy while no recovery was deteced within 8 weeks after irradiation to 20 Gy. These results suggest that careful attention must be paid to liver not to be included in exposure field in radiation therapy.

• 한국식품영양학회지
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• 제11권1호
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• pp.114-122
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• 1998

표고버섯의 열수 가용성 다당류(PS)와 간 장해물질이며 발암성을 갖는 butter yellow를 흰쥐의 식이에 6주간 투여하여 혈장과 간의 효소활성 및 과산화지질의 함량을 측정한 결과는 다음과 같다. 1. 혈장 중 GOT 활성은 better yellow 첨가군(BO군)보다 PS의 투여군(BP군 : BO+PS)이 비교적 낮은 수준을 나타내었으며 ${\gamma}$-GTP와 GPT 활성에서도 BO군에 비하여 BP군에서 유의하게 낮은 활성을 나타냈다. 2. 간장 중 glutathion peroxidase, catalase 및 lactate dephdregenase 활성 또한 기초식이군(NO군)에 PS를 토여한 군(NP군)은 NO군과 유의한 차이가 없었으나 BP군은 BO군에 비하여 유의하게 낮은 수준이었다. SOD활성은 각 군간에 유사한 수치로 유의적인 차리를 보이지 않았다. 3. 간장의 microsomal 분획분 및 cytosol 분획분 중의 glutathione S-transferase 활성도는 BP군이 BO군보다 유의하게 낮은 수준을 보였으나 better yellow를 첨가하지 않은 NO군에 비하여 상당히 높은 수준을 나타내었다. 4. 간장 내 glutathione과 malondialdehyde의 함량 또한 PS의 투여로 생성이 저하되는 것으로 나타났다. 이상의 결과를 보아 표고버섯의 열수 추출물은 생체조직의 지질의 과산화를 방지하므로써 better yellow에 의한 간장장애의 치료 또는 독성을 완화하는 효과가 있는 것으로 사료된다.

• 한국환경보건학회지
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• 제29권2호
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• pp.80-86
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• 2003

To evaluate the effect of cyclohexane(CH) treatment on the serum levels of glutathion S-transferase(GST) activity in liver damaged animals, damaged liver was induced with pretreatment of 50% $CCl_4$ dissolved in olive oil (0.1 m1/100g body weight) intraperitoneally 17 times every other day. To $CCl_4$-treated rats, CH (1.56 g/kg body weight, i.p) was injected once and then the animals were sacrificed at 4 hours after injection of CH. The $CCl_4$-treated animals were identified as severe liver damage on the basis of liver functional findings, 1,e, increased serum levels of alanine aminotransferase(ALT), alkaline phosphate(ALP) and xanthine oxidase(XO) activities. On the other hand, $CCl_4$-treated animals injected with CH once($CCl_4$-pretreated animals) showed more decreased serum levels of ALT and XO, and more increased those of ALP rather than $CCl_4$-treated animals. In case of comparing the GST with ALT activity in liver, both $CCl_4$-treated and pretreated animals showed similar changing pattern of enzyme actvity. Especially $CCl_4$-pretreated animals showed significantly increased serum level of GST actvity compared with the $CCl_4$-treated those, whereas those of ALT showed reversed tendency. In aspects of GST enzyme kinetics, $CCl_4$-pretreated animals showed higher Vmax of liver GST enzyme than $CCl_4$-treated animals. In conclusion, injection of CH to the liver damaged rats led to enhanced liver damage and more increased activity of serum GST which may be chiefly caused by the enzyme induction.

• 한국의학물리학회지:의학물리
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• 제4권2호
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• pp.69-76
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• 1993

방사선이 간 담도계에 미치는 영향을 검토하기 위하여 선형가속장치에 의한 4MV X선 10, 15 및 20Gy를 흰쥐의 간 일부에 각각 조사한 후 1, 2, 4 및 8주에 간 담도계 손상의 지표로 이용 되고 있는 혈중 alkaline phosphatase 및 bilirubin의 함량을 측정 검토한 결과 먼저 alkaline phosphatase의 활성은 전 조사선량군에서 상승경향을 나타내었으며, 특히 20 Gy 8주군에서 현저한 상승을 나타내었고, 혈중 bilirubin의 함량도 시간 경과와 함께 상승경향을 나타내었으며, 20 Gy 8주군은 유의하게 상승하였다. 이상의 실험 성적을 종합해 볼때 방사선 조사로 인한 간 담도계의 장해는 조사된 선량에 따라 10 및 15 Gy군에서는 기간 경과와 함께 점차 회복되는 경향이었으나, 20 Gy군에서는 오히려 증가 된 것으로 보아 부분조사에서도 20 Gy가 한계선량임을 확인할 수 있었으며, 방사선치료시 간의 일부가 조사야에 포함되지 않도록하는 주의가 필요하다고 생각된다.

• 생명과학회지
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• 제11권2호
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• pp.126-132
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• 2001

Effect of Quartz porphyty(QP) on functional and morphological changes of the liver and kidney was studied in male common finch and white java sparrow fed with the basal diet(Control group) or experimental diet containing 3.0% QP(QP group) for 14 days. There was not significantly different morphological change of the liver upon light microscopic examination in common finch and white java sparrow between control group and QP group. Morphological change of renal tissue upon light microscopic examination in common finch and white java sparrow was not also significantly different between control group and QP group. The concentrations of serum creatinine, blood urea nitrogen, and nric acid as renal functional parameters of common finch and white java sparrow were not significantly different in the both groups. The activity of glutamic oxaloacetic transaminase(GOT) as hepatic functional parameter in common finch was significantly higher in the QP group($\rho$<0.05), whereas the activity of glutamic pyruvic transaminase(GPT) as hepatic functional parameter in common finch was not significantly different in the both groups. The activities of GOP and GPT in white java sparrow were not significantly different in the both groups. The morphologic findings and functional parameters of the liver and kidney observed in common finch and white java sparrow fed with 3.0% QP diet showed evidence of slightly liver damage accompanied with increased release of enzyme and fatty change of the hepatocytes in common finch, suggested that the tissues in some animals can be damaged by feeding a diet supplemented with 3.0% QP.

• 한국응용과학기술학회지
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• 제35권2호
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• pp.485-491
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• 2018

본 연구는 동결 건조한 krill (Euphausia superba) meal을 함량별 섭취시켰을 때 7주령된 Sprague Dawley계 수컷 흰쥐의 혈청 중 alkaline phosphatase (ALP), aminotransferase (AST, ALT) 및 lactate dehydrogenase (LDH) 등 혈청 간 기능 효소활성과 장기 조직의 불소 함량에 미치는 영향을 확인하였다. 기본식이를 급여한 대조군인 CG군을 비롯하여 10%, 20%, 30%의 krill meal을 첨가한 급여군을 각각 KM10군, KM20군, KM30군으로 구분하여 4군의 급여군으로 나누어 5주간 실험 사육한 결과는 다음과 같다. 혈청 중 alkaline phosphatase (ALP), aminotransferase (AST, ALT) 및 lactate dehydrogenase (LDH)의 효소 활성은 대조군인 CG군 보다 krill meal을 함량별 첨가 급여군에서 감소되는 것으로 나타났다. 흰쥐의 혈청 및 장기조직(간, 뇌, 심장, 신장)의 불소 함량은 krill meal의 함량에 따라 불소 함량도 증가되는 것으로 나타났다.

• Biomolecules & Therapeutics
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• 제14권4호
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• pp.226-234
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• 2006

Disturbance of antioxidant system is very common in chronic alcoholics and herbal or natural products with antioxidant activity have been used for its treatment. This study was to investigate the effect of Vitis vinifera extract(V), Schisandra chinensis extract(S), Taraxacum officinale extract(T), Gardenia jasminoides extract(G), Angelica acutiloba extract(A) and Paeonia japonica extract(P), and their combinations on the antioxidant and ethanol oxidation system. Male Sprague-Dawley rats were subjected to Lieber-DeCarli ethanol liquid diet(ED) and were then given different herbal extract mixtures for 6 weeks including VST(V 100+S 150+T 150mg/kg/day), VSG(V 100+S 150+G 150mg/kg/day), VTG(V 100+T 150+G 150mg/kg/day), and VAP(V 100+A 150+P 150mg/kg/day). When the activity of alcohol dehydrogenase(ADH) and acetaldehyde dehydrogenase(ALDH) were compared between ED only group and herbal extracts treatment group, the differences were statistically significant. Phase I and II(glutathione-S-transferase, phenol sulfatransferase) enzyme activities were found to be significantly higher in the VAT treatment group compared to the ED group. Herbal extracts not only repressed the ethanol-induced elevation of malondialdehyde level, but also protected against ethanol-induced decrease in glutathione content, glutathione reductase, glutathione peroxidase, catalase and superoxide dismutase activities. The administration of the herbal extracts was found to be effective in eliminating lipid-peroxides induced by long-term consumption of alcohol by activating various enzyme systems and physiological active compound formation system. After a chronic consumption of alcohol, Angelica Radix protected the liver via activating the ethanol-metabolism enzyme system, and Paeoniae Radix via activating the ethanol-metabolism enzyme and the phase I, II-metabolism enzyme system. Taraxaci Herba was also effective in liver protection via activating the ethanol-metabolism enzyme system and the phase I, II-metabolism enzyme system, Gardeniae Fructus via activating the phase II-metabolism enzyme system and the anti-oxidation system enzyme, and Schisandra Fructus and a grapestone via activating the anti-oxidation system. Our data suggest that these herbal extracts may be useful as a health functional food or new drug candidate for fatty liver and hepatotoxicity induced by chronic alcohol consumption.

• Nutrition Research and Practice
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• 제5권5호
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• pp.429-434
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• 2011

Korean raspberry, Rubus coreanus Miquel (RCM), contains high concentrations of phenolic compounds, which prevent oxidative stress. To determine the effect of RCM on antioxidant capacity in humans, we assessed in vivo lipid oxidation and antioxidant enzyme activities from plasma in 15 healthy men. The subjects ingested 30 g of freeze-dried RCM daily for 4 weeks. Blood was taken at baseline and at the end of the study to determine blood lipid profiles, fasting plasma glucose, liver function, lipid peroxidation, and antioxidant enzyme activities. RCM supplementation had no effect on blood lipid or fasting plasma glucose concentrations but decreased alkaline phosphatase activity. RCM supplementation increased glutathione peroxidase activities (P<0.05) but had no effect on lipid peroxidation. These results suggest that short-term RCM supplementation may offer health benefits by enhancing antioxidant capacity in a healthy population.

• 한국환경과학회지
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• 제28권11호
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• pp.943-950
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• 2019

The purpose of this study was to probe the influences of NaF oral administration on a dose-effect relationship between fluoride levels of serum enzyme activity such as alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) in rats fed experimental diets for 5 weeks. All groups increased the activity of serum ALP, AST, ALT, and LDH levels with increasing NaF. In addition the fluoride levels of serum and organ tissues (liver, brain, heart, lung, kidney) in oral NaF groups (NF3~NF50) were significantly increased by adding sodium fluoride in comparison with normal diet group (ND) (p<0.05). These results, a high concentration of sodium fluoride was determined that the toxicity to various organ tissues.