• Biotechnology and Bioprocess Engineering:BBE
• /
• v.5 no.1
• /
• pp.17-22
• /
• 2000

The separation of tryptophan enantiomers was carried out with medium-pressure liquid chromatography using BSA (bovine serum albumin)-bonded silica as a chiral stationary phase. The influence of various experimental factors such as pH and ionic strength of mobile phase, separation temperature, and the presence of organic additives on the resolution was studied. In order to expand this system to preparative scale, the loadability of sample and the stability of stationary phase for repeated use were also examined. The separation of tryptophan enantiomers was successful with this system. The data indicated that a higher separation factor (a) was obtained at a higher pH and lower temperature and ionic strength in mobile phase. Addition of organic additives (acetonitrile and 2-propanol) in mobile phase contributed to reduce the retention time of L-tryptophan. About $30\%$ of the separation factor was reduced after 80 days of repeated use.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.14 no.3
• /
• pp.257-262
• /
• 2001

Single crystalline films of La doped YIG(yttrium iron garnet) were grown by the liquid phase epitaxy. The lattice constants of films obtained by DCD(double crystalline diffractometer) measurement increased with increasing La contents in films. In particular, lattice constants of films grown wiht Y/La=20 solution were nearly same as those of GGG (gadolinium gallium garnet) substrate. The saturation magnetization measured with VSM (vibrating sample magnetometer) was about 1750Gauss which is the same as that of pure YIG irrespective of La contents in films. FMR(ferromagnetic resonance) linewidth of La doped YIG was smaller than that of pure YIG. Since appropriate La doping decreases the lattice mismatch between film and substrate, the FMR linewidth was Y/La=20 in this experiment.

• Textile Coloration and Finishing
• /
• v.23 no.2
• /
• pp.146-153
• /
• 2011

Silver nano-particles have been synthesized by liquid phase reduction method and alcohol reduction process. Silver nano-particles of the size 30 ~ 40 nm were formed successfully by alcohol reduction process. The formation, structure, morphology and size of silver nano-particles have been studied using FE-SEM, TEM, XRD, UV-visible spectroscopy. In particular high dispersion stability of the synthesized silver nano-particles could be obtained by PVP binding. Antibacterial activity of Ag/PET master batch sample made from its nano-silver particles showed excellent antibacterial activity against S. aureus and E. coli.

• Analytical Science and Technology
• /
• v.11 no.6
• /
• pp.499-504
• /
• 1998

An analytical method for the simultaneous measurement of trace Cu, Sn, and Bi in sea water has been investigated by Inductively Coupled Plasma-Mass Spectrometry. Amberlite IRC-718 resin was used as a solid phase in solid-liquid extraction technique for the removal of matrix interferences such as Na, S, P, and other polyatomic ion species. Recoveries of 99.8% for Cu, 99.6% for Sn, and 97.9% for Bi were obtained for the standard spiked sample. The developed method was applied to analysis of trace metals in sea water.

• Bulletin of the Korean Chemical Society
• /
• v.25 no.9
• /
• pp.1336-1340
• /
• 2004

The same kind of chiral stationary phase with a commercialized chiral column was used to make preparative chiral columns and was applied to resolve racemic N-acetyl-1-naphthylethylamide (3) by preparative liquid chromatography. An improved chromatographic condition to resolve racemic 3 on the CSP was examined by changing flow rate and kind of the mobile phase and the sample injection volume. The optimized separation conditions were applied to resolve racemic 1,1'-Binaphthyl-2,2'-diamine(4).

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2000.11a
• /
• pp.89-92
• /
• 2000

Liquid Phase Epitaxy 법을 이용하여 La이 첨가된 YIG(Yitrium Ion Garnet)막을 성장시켰다. X선 회절 분석을 이용하여 La의 첨가량을 변화시키며 제조된 막의 격자상수를 조사한 결과, La의 첨가량이 증가함에 따라 성장된 막의 격자상수도 증가하였으며 Y/La이 20인 경우, 막의 격자상수가 기판으로 사용한 GGG의 격자상수와 일치하였다. VSM(Vibration Sample Magnetometer)를 이용하여 구한 막의 포화자화 값은 La의 첨가량과 관계없이 순수한 YIG의 경우와 같은 값인 1750정도로 거의 일정하였다. FMR(Ferro Magnetic Resonance) 측정장치를 이용한 막의 강자성 공명선폭을 측정결과 막의 공명선폭은 La의 첨가량과 관계없이 모든 경우에 순수한 YIG보다 감소하였다. 실험범위내의 La의 첨가에 대해서 기판과의 격자불일치가 순수한 YIG의 경우보다 감소하기 때문이다. La의 첨가량이 많은 조건에서 성장시킨 막은 공명선폭이 크고 두께의 증가에 따라서 선폭이 증가하였으며, Y/La가 20과 30일 때 성장시킨 막에서는 공명선폭의 절대값도 작고 두께에 따른 공명선폭의 변화도 관찰되지 않았다.

• Bulletin of the Korean Chemical Society
• /
• v.19 no.6
• /
• pp.617-618
• /
• 1998

A simple and reproducible pretreatment method was developed for the determination of dioxins in milk sample. Liquid-liquid extraction (LLE) was used for the initial extraction of the analyte from milk. For the elimination of interferences coextracted from milk, acid treatment followed by multilayer silica gel, and then alumina column clean-up were performed. The clean extract could be obtained without carbon column or high performance liquid chromatographic (HPLC) clean-up procedure. Polychlorinated biphenyles (PCBs) and dioxins were separated on neutral alumina activated at 180 ℃ for 12 hours. The final extract was analyzed by HPLC and high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS). The recovery of dioxins spiked in milk at 75-300 ppt level was 83.3-98.9% and their relative standard deviation was 4.1-14%.

• Bulletin of the Korean Chemical Society
• /
• v.19 no.6
• /
• pp.619-624
• /
• 1998

A simple and reproducible pretreatment method was developed for the determination of dioxins in milk sample. Liquid-liquid extraction (LLE) was used for the initial extraction of the analyte from milk. For the elimination of interferences coextracted from milk, acid treatment followed by multilayer silica gel, and then alumina column clean-up were performed. The clean extract could be obtained without carbon column or high performance liquid chromatographic (HPLC) clean-up procedure. Polychlorinated biphenyles (PCBS) and dioxins were separated on neutral alumina activated at 180 ℃ for 12 hours. The final extract was analyzed by HPLC and high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS). The recovery of dioxins spiked in milk at 75-300 ppt level was 83.3-98.9% and their relative standard deviation was 4.1-14%.

• Journal of the Optical Society of Korea
• /
• v.18 no.3
• /
• pp.251-255
• /
• 2014

A fiber diffuser sheet based on poly (ethylene naphthalate) (PEN) and poly (methylpentene) (PMP) has shown potential for liquid crystal display backlight units, but these materials have an interfacial adhesion problem. To improve the interfacial adhesion between the fibers and matrix components, we have proposed the use of amorphous poly (cyclohexane-1,4-dimethylene terephthalate) (Tritan) instead of PEN. Furthermore, the fabrication processes have been optimized and simplified to improve the optical and mechanical properties of the sheet. As a result, the most effective fiber content for achieving the best haze characteristics of a sample consisting of Tritan and PMP has been identified.

• The Journal of Dong Guk Oriental Medicine
• /
• v.2 no.1
• /
• pp.107-125
• /
• 1993

This study was performed to investigate the effects of Gwaluzisiltang and Gwaluzisiltanggami on the intravascular coagulation. The experimental group divided two groups : one group was the intravascular coagulation induced by endotoxin in rats, another group was the formation of paw edema by contusion in rats, and then these rats were treated with liquid extract of Gwaluzisiltang(Sample I) and Gwaluzisiltanggami(Sample II), which were administered orally. Then the numbers of platelets, concentration of fibrinogen, Prothrombin time and FDP(fibrin-fibrinogen degradation Products)were measured. The results were as follows : 1. The effects of the Intravascular coagulation 1) Platelet was increased significantly in the sample I compared with control group. 2) Fibrinogen was increased significantly in the sample I compared with control group. 3) Prothrombin time was shortened significantly in the sample I and more shortened in the sample II compared with control group. 4) FDP was decreased significantly in the sample I and more decreased in the samplem II compared with control group. 2. The effect of the formation of paw edema by contusion in rats. 1) The rate of paw edem was decreased significantly after five hours in the sample I 2) Platelet was increased significantly in the sample I compared with control group. 3) Fibrinogen was decreased in the sample I and sample II compared with control group, but it is not significant 4) Prothrombin time was shortened significantly in the sample II compared with control group. According to the above results, it is considered that the Gwaluzisiltang and Gwaluzisiltanggami seem to be applicable disease related to thrombosis, because they obtained significant effects on the experimental method which are based on the oriental medical theory-the principle of phlegm and blood stasis have the same source and disease (痰瘀同源, 痰瘀同病).