• YAKHAK HOEJI
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• v.42 no.2
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• pp.149-152
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• 1998

A high performance liquid chromatographic method was developed for the determination of DA-6034 in biological fluids using internal standard. Plasma containing DA-6034 and inter nal standard was extracted by liquid-liquid extraction at an acidic pH. After evaporation of the organic layer, the drug and internal standard were reconstituted with mobile phase and injected into the column. They were separated by high performance liquid chromatography on inertsil ODS II column at 334 nm. The detection limit of DA-6034 in plasma was 0.02 ${\mu}$g/ml. In this method, the range of recovery and coefficients of variation were 96-110% and 0.40-3.78%, respectively. There was no interference from endogenous substances. Urine and bile were analysed using the deproteinization method and the detection limit of DA-6034 was 1${\mu}$g/l.

• Structural Engineering and Mechanics
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• v.9 no.3
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• pp.227-240
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• 2000

The study of the optimal damping ratio of a tuned liquid-column damper (or TLCD) attached to a single-degree-of-freedom system is presented. The tuned liquid-column damper is composed of two vertical columns connected by a horizontal section in the bottom and partially filled with water. The ratio of the length of the horizontal section to the effective wetted length of a TLCD considered as another important parameter is also presented for investigation. A simple pendulum-like model test is conducted to simulate a long-period motion in order to prove the effectiveness of TLCD for vibrational control. Comparisons of the experimental and analytic results of the TLCD, TLD (tuned-liquid damper), and TMD (tuned-mass damper) are included for discussion.

• Structural Engineering and Mechanics
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• v.33 no.3
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• pp.339-355
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• 2009

The sealed, tuned liquid column gas damper (TLCGD) with gas-spring effect extends the frequency range of application up to about 5 Hz and efficiently increases the modal structural damping. In this paper the influence of several TLCGDs to reduce coupled translational and rotational vibrations of plan-asymmetric buildings under wind or seismic loads is investigated. The locations of the modal centers of velocity of rigidly assumed floors are crucial to select the design and the optimal position of the liquid absorbers. TLCGD's dynamics can be derived in detail using the extended non-stationary Bernoulli's equation for moving reference systems. Modal tuning of the TLCGD renders the optimal parameters by means of a geometrical transformation and in analogy to the classical tuned mass damper (TMD). Subsequently, fine-tuning is conveniently performed in the state space domain. Numerical simulations illustrate a significant reduction of the vibrations of plan-asymmetric buildings by the proposed TLCGDs.

• Bulletin of the Korean Chemical Society
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• v.19 no.6
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• pp.617-618
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• 1998

A simple and reproducible pretreatment method was developed for the determination of dioxins in milk sample. Liquid-liquid extraction (LLE) was used for the initial extraction of the analyte from milk. For the elimination of interferences coextracted from milk, acid treatment followed by multilayer silica gel, and then alumina column clean-up were performed. The clean extract could be obtained without carbon column or high performance liquid chromatographic (HPLC) clean-up procedure. Polychlorinated biphenyles (PCBs) and dioxins were separated on neutral alumina activated at 180 ℃ for 12 hours. The final extract was analyzed by HPLC and high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS). The recovery of dioxins spiked in milk at 75-300 ppt level was 83.3-98.9% and their relative standard deviation was 4.1-14%.

• Bulletin of the Korean Chemical Society
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• v.19 no.6
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• pp.619-624
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• 1998

A simple and reproducible pretreatment method was developed for the determination of dioxins in milk sample. Liquid-liquid extraction (LLE) was used for the initial extraction of the analyte from milk. For the elimination of interferences coextracted from milk, acid treatment followed by multilayer silica gel, and then alumina column clean-up were performed. The clean extract could be obtained without carbon column or high performance liquid chromatographic (HPLC) clean-up procedure. Polychlorinated biphenyles (PCBS) and dioxins were separated on neutral alumina activated at 180 ℃ for 12 hours. The final extract was analyzed by HPLC and high resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS). The recovery of dioxins spiked in milk at 75-300 ppt level was 83.3-98.9% and their relative standard deviation was 4.1-14%.

• Korean Journal of Food Science and Technology
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• v.22 no.1
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• pp.88-93
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• 1990

For effective separation of the N-TFA n-butyl ester amino acids on the stainless steel column by GLC, dual column of the mixed stationary phases, 3.36% OV-17+3.0% SE-30(column 1) and 1% NPGS +0.5% OV-17+0.5% SE-30(column 2) on chromosorb W HP 100-120 mesh, were used. On the column 1. the nineteen amino acids except histidine were obtained. However, alanine and valine peaks were not separated by this column. On the column 2, the sixteen amino acid peaks showed good separation, but tryptophan. arginine, histidine, and tyrosine peaks were not obtained. Calibration graphs for all amino acids obtained by the plotting the ratios of their peaks hights to that of internal standard versus the micro mole of the amino acids in the range $1.25{\times}10^{-3}{\mu}mol-1.0{\times}10^{-2}{\mu}mole$ showed linearity and passed through the origin.

• Archives of Pharmacal Research
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• v.23 no.5
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• pp.441-445
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• 2000

Achiral-chiral column switching HPLC assay was developed to allow the separation and quantification of the enantiomers of terbutaline in human plasma by means of fluorescence detection. Plasma samples were prepared by solid-phase extraction with sep-pak silica, followed by HPLC assay. The enantiomers of terbutaline and the internal standard were separated from the biological matrix on a silica column, and the two enantiomers were resolved and quantified on a Sumichiral OA-4900 column. The two columns were connected by a switching valve equipped with silica trap column, The trap column was used to concentrate the terbutaline in the eluent from the achiral column before back flushing onto the chiral phase. For each enantiomers, the assay was linear between 2.5-125 ng/$m\ell$ (r=0.9999) and detection limit was 1.0 ng/$m\ell$ .

• Environmental Engineering Research
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• v.19 no.4
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• pp.345-354
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• 2014

In this paper, effects of physical configurations and operating conditions on bubble column performance were analyzed in terms of bubble hydrodynamic and mass transfer parameters. Bubble column with 3 different dimensions and 7 gas diffusers (single / multiple orifice and rigid / flexible orifice) were applied. High speed camera and image analysis program were used for analyzing the bubble hydrodynamic parameters. The local liquid-side mass transfer coefficient ($k_L$) was estimated from the volumetric mass transfer coefficient ($k_La$) and the interfacial area (a), which was deduced from the bubble diameter ($D_B$) and the terminal bubble rising velocity ($U_B$). The result showed that the values of kLa and a increased with the superficial gas velocity (Vg) and the size of bubble column. Influences of gas diffuser physical property (orifice size, thickness and orifice number) can be proven on the generated bubble size and the mass transfer performance in bubble column. Concerning the variation of $k_L$ coefficients with bubble size, 3 zones (Zone A, B and C) can be observed. For Zone A and Zone C, a good agreement between the experimental and the predicted $K_L$ coefficients was obtained (average difference of ${\pm}15%$), whereas the inaccuracy result (of ${\pm}40%$) was found in Zone B. To enhance the high $k_La$ coefficient and absorption efficiency in bubble column, it was unnecessary to generate numerous fine bubbles at high superficial gas velocity since it causes high power consumption with the great decrease of $k_L$ coefficients.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2007.11a
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• pp.121-125
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• 2007

LCVA has an advantage that its natural frequency can be easily controlled by changing the area ratio of the vertical column and horizontal part. The previous studies investigated the dynamic characteristics of the LCVA under harmonic load. This study experimentally obtained the first and second mode natural frequencies of the LCVA from shaking table tests using white noise and compared the values with the ones by previous study. Test results show that the measured first mode natural frequency of the LCVA is larger than the calculated one when the area ratio is larger than 1. The second mode frequency increases with the increasing area ratio, which is due to the sloshing motion effect resulting from the large area of the vertical column.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2008.04a
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• pp.653-658
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• 2008

LCVA has an advantage that its natural frequency can be easily controlled by changing the area ratio of the vertical column and horizontal part. The previous studies investigated the dynamic characteristics of the LCVA under harmonic load. This study experimentally obtained the first and second mode natural frequencies of the LCVA from shaking table tests using white noise and compared the values with the ones by previous study. Test results show that the measured first mode natural frequency of the LCVA has a different value compared with calculated one. The effective length($L_e$) was revised using by power equation. In the Case01 to 19, the standard deviation($s_r$) is 4.7292 and the coefficient of correlation(r) is 0.9856. In the Case21 to 61, the standard deviation ($s_r$) is 14.2143 and the coefficient of correlation(r) is 0.9935. The second mode frequency increases with the increasing area ratio, which is due to the sloshing motion effect resulting from the large area of the vertical column.