• Ocean and Polar Research
• /
• 제32권2호
• /
• pp.165-175
• /
• 2010

Dietary lipid biomarkers (fatty acids, fatty alcohols, and sterols) in adult specimens were analyzed to compare and understand the feeding ecology of the euphausiid, Euphausia pacifica, from three geographically and environmentally diverse Korean waters (Yellow Sea, East China Sea, and East Sea). Total lipid content of E. pacifica from Korean waters was about 10% dry weight (DW) with a dominance of phospholipids (>46.9% of total lipid content), which are known as membrane components. A saturated fatty acid, C16:0, a monounsaturated fatty acid, C18:1(n-9), and two polyunsaturated fatty acids, C20:5(n-3) and 22:6(n-3), were most abundant (>60% of total fatty acids) in the fatty acid composition. Some of the fatty acids showed slight differences among regions although no significant compositional changes of fatty acids were detected between these regions. Phytol, originating from the side chain of chlorophyll and indicative of active feeding on phytoplankton, was detected all samples. Trace amounts of various fatty alcohols were also detected in E. pacifica. Specifically, krill from the Yellow Sea showed relatively high amounts of longchain monounsaturated fatty alcohols (i.e. 20:1 and 22:1), generally found in herbivorous copepods. Three different kinds of sterols were detected in E. pacifica. The most dominant of these sterols was cholest-5-en-$3{\beta}$-ol (cholesterol). The lipid compositions and ratios of fatty acid trophic markers are indicative of herbivory in E. pacifica from the Yellow Sea and East Sea (mainly feeding on dinoflagellates and diatoms, respectively). The lipid compositions and ratios of fatty acid trophic markers are indicative of carnivory or omnivory in E. pacifica from the East China Sea, mainly feeding on microzooplankton such as protozoa. In conclusion, lipid biomarkers provide useful information about krill feeding type. However, further analyses and experiments (i.e. gut content analysis, in situ grazing experiment, etc.) are needed to better understand the feeding ecology of E. pacifica in various marine environments.

• Preventive Nutrition and Food Science
• /
• 제16권3호
• /
• pp.202-209
• /
• 2011

This study was initiated to investigate the effects of lyophilized purslane (Portulaca oleracea) powder (5% and 10%) on lipid levels in rats fed a hypercholesterolemia-inducing diet (1% cholesterol). During the four week study, there were no significant differences in either weight change or the food efficiency ratio between the group fed the hypercholesterol diet alone (HC) and the groups fed the purslane powder (HCPO-I and HCPO-II, 5% and 10% purslane, respectively). In serum, the levels of total lipid, total cholesterol and LDL-C decreased significantly for the group fed the 10% purslane powder (HCPO-II) in comparison with the group fed the 5% purslane powder (HCPO-I). The atherogenic index (AI) was reduced by about 51% for the group fed the 10% purslane powder (1.47) in comparison with the HC group (3.03). The activities of GOT, GPT, ALP and LDH decreased significantly for the groups fed the purslane powder in comparison with the HC group. Regarding liver tissue, the levels of total lipid, total cholesterol and triglyceride decreased significantly for the purslane powder-fed rats compared to the HC group. The fecal lipid profiles increased significantly as the amount of purslane powder was increased. Compared to the HC group, the fecal total cholesterol and triglyceride levels were higher in the group fed the 10% purslane powder by about 2.8 times and 2.3 times, respectively. For the serum and liver tissue, the content of lipid peroxide decreased significantly in the groups fed purslane powder compared to the HC group. The data from this experiment show an increase in the lipid levels discharged in feces, suggesting that the supplementation of purslane powder to a hypercholesterolemia-inducing diet reduces lipid levels.

• 대한화장품학회:학술대회논문집
• /
• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
• /
• pp.508-518
• /
• 2003

SZ95 cell is an immortalized human sebaceous gland cell line that shows the morphologic, phenotypic and functional characteristics of normal human sebocytes. Sebocytes may play crucial parts in the pathophysiologic processes and disorders of the pilosebaceous unit. The secretory activity of the sebaceous gland is remarkably species-specific and acne is an exclusively human disease. Thus, this SZ95 cells offer possibilities for investigations on the physiology of the sebaceous gland and its role in sebum-associated skin disease such as acne. In this study, we investigated the effects of 13-cis-retinoic acid (13-cis-RA) and spironolactone, frequently used as therapeutic agents of acne, on the lipid synthesis and proliferation of human sebocytes. Cell proliferation was determined by MTT assay and cytoplasmic lipid droplets was shown by Oil-red a staining. Total lipid levels were biochemically estimated by the sulfo-phospho-vanilline reagent. 13-cis-RA and spironolactone significantly inhibited proliferation and lipid levels in a dose-dependent manner. Combined treatment with testosterone and 13-cis-RA or spironolactone resulted in a lower total lipid levels than that with androgen alone. These observations indicate that 13-cis-RA and spironolactone are potent inhibitors of both cell proliferation and lipid synthesis in human sebocytes. We will provide experimental evidence that this human sebocyte cell line serves as an adequate tool for evaluating the anti-lipogenic activity of various compounds potentially useful for the bioactive cosmeceutical ingredients on acne skin, and studying the intracellular biochemical markers depending on the types of compounds from various sources.

• Food Science and Biotechnology
• /
• 제15권3호
• /
• pp.399-403
• /
• 2006

The effect of flour storage conditions on the lipid oxidation of fried products during storage was studied. Wheat flour was stored at $60^{\circ}C$ in the dark and at water activity (Aw) of 0.3, 0.5, or 0.8 for 21 days. The square-shaped dough ($2{\times}2{\times}0.1\;cm$) made with the stored flour and water was fried in soybean oil at $160^{\circ}C$ for 1 min. The fried products were stored at $60^{\circ}C$ for 15 days in the dark. The degree of lipid oxidation of the fried products was evaluated by conjugated dienoic acid (CDA) content and p-anisidine value (PAV). Both CDA content and PAV of the fried products increased with lengthening storage time of the fried products, suggesting that longer storage of the fried products raised the lipid oxidation. Furthermore, the lipid oxidation of the fried products made with flour that had been stored for a longer time tended to be higher than that of those made with unstored or short-term-stored flour. However, Aw at which the flour was stored did not significantly affect the lipid oxidation of either flour or the fried products during storage. The storage time of flour clearly exerted a greater effect than Aw on the lipid oxidation of the fried products during storage at $60^{\circ}C$ in the dark. This suggests that for the storage stability of fried products, the flour storage time is a more important factor than Aw at which the flour is stored.

• 약학회지
• /
• 제25권4호
• /
• pp.181-186
• /
• 1981

Compositions of neutral lipid of the hazel nut (Corylus heterophylla Fischer var. thumbergii Blume, Betulaceae) produced in Korea were analyzed by thinchrography (Iatrosoan TH-10 type), which was equipped with a flame ionization detector. The neutral lipid components were nearly separated of the basis of their degree of unsaturation by employing the chromatography on silica gel sintered rod impregnated with 12.5% silver nitrate and it was noted that triglyceride was the major lipid components of Korean hazel nut. The triglyceride compositions were trilinolenin 10.63, trilinolein 18.01, triolein 42.24, tripalmitin 17.57 and tristearin 11.55% by thinchrographic analysis.

• Journal of Nutrition and Health
• /
• 제22권1호
• /
• pp.32-35
• /
• 1989

Serum lipid peroxide levels in 25 chronic renal failure patients undergoing hemodialysis were examined by determining TBA reaction with spectrofluorometry. The lipid peroxide levels, 208.9$\pm$88.4nmol/ml, in the patient group was significantly higher than 152.4$\pm$43.9nmol/ml of 48 control healthy subjects. It is likely that the elevated serum lipid peroxide levels can play a role in increasing tendency of hemorrhage and incidence of atherosclerosis in chronic renal failure patients.

• Journal of Veterinary Science
• /
• 제25권1호
• /
• pp.14.1-14.5
• /
• 2024

An adult female ringed seal died suddenly and was subsequently examined for diagnostic purposes. The animal's lungs demonstrated mild non-collapsibility and multifocal white to yellow patches. Histopathological examination revealed multifocal pulmonary histiocytosis. Alveoli were filled with numerous foamy macrophages cytoplasm and scattered multinucleated giant cells containing cholesterol clefts. The foamy cytoplasm of the macrophages stained with oil red O stain. Further, lipid droplets within the cytoplasm were detected by electron microscopy. To the author's knowledge, this is the first case report describing the histochemical staining and electron microscopic findings associated with endogenous lipid pneumonia in ringed seal.

• 한국식품조리과학회지
• /
• 제12권3호
• /
• pp.295-299
• /
• 1996

비방사구와 방사구 달걀 난황을 가열 압착하여 얻은 난황유를 정제한 후 화학적 성질과 지질조성에 관하여 실험한 결과는 다음과 같다. 난황의 일반성분은 비방사구, 방사구가 각각 수분이 49.50%, 47.06%,조단백질은 16.53%, 16.98%,조 지방은 31.05%, 33.34%로 주성분을 이루었고, 조지방, 조단백의 함량은 방사구가 비방사구 보다 더 높았다 난황유의 화학적 성질은 비방사구, 방사구가 각각 산가 8.95, 9.85, 요오드가 57.64, 58.15, 비누가 240.14, 223.92로. 나타났다. 난황유 총지질의 조성은 비방사구, 방사구 각각 중성지질 76.60%, 71.23%,당지질 3.95%, 5.03%, 인지질 19.45%, 23.74%였다. 중성지질 중에는 비방사구, 방사구 각각 triglycride가 59.3%, 56.3%로 주성분을 이루었고 그 외에 monoglyceride와 diglyceridr의 함량이 높았다. 당지질은 비방사구, 방사구 각각 digalactosyl diglyceride가 98.3%, 97.8%로 대부분을 차지 하였다. 인지질은 방사구, 비방사구 각각 phosphatidiyl choline + phosphatidyl serine이 58.6%, 59.8%로 주성분을 이루었고, 그 외에 lecithin + sphingomyelin과 미확인 성분이 존재하였다.

• Bulletin of the Korean Chemical Society
• /
• 제34권7호
• /
• pp.2143-2147
• /
• 2013

Matrix assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is a very effective method for lipid mass fingerprinting. However, MALDI MS suffered from spectral complexities, differential ionization efficiencies, and poor reproducibility when analyzing complex lipid mixtures without prior separation steps. Here, we aimed to find optimal MALDI sample preparation methods which enable selective or class-wide mass fingerprinting of two totally different lipid classes. In order to achieve this, various matrices with additives were tested against the mixture of phosphatidylcholine (PC) and cerebrosides (Cers) which are abundant in animal brain tissues and also of great interests in disease biology. Our results showed that, from complex lipid mixtures, 2,4,6-trihydroxyacetophenone (THAP) with $NaNO_3$ was a useful MALDI matrix for the class-wide fingerprinting of PC and Cers. In contrast, THAP efficiently generated PC-focused profiles and graphene oxide (GO) with $NaNO_3$ provided Cer-only profiles with reduced spectral complexity.

• 한국식품영양과학회지
• /
• 제20권1호
• /
• pp.72-77
• /
• 1991

This study was designed to obseve the effect of defatted perilla as a dietary fiber on lipid components of serum feces and liver in rats. Serum cholesterol and triglyceride were signfican-ly decreased in defatty perilla added groups. total lipid cholesterol and triglyceride of feces were significantly increased in defatted perilla added groups. Liver phospholipid content was higher in the defatted perilla added groups. The other lipid components of the liver were not affected by the defatted perilla administration. These results suggest the possibitlity that defatted perilla have a reducing effect of serum cholesterol and triglyceride through the incre-ment of feacal excretion of lipid component and phospholipid in liver.