• 대한의생명과학회지
• /
• 제29권4호
• /
• pp.231-241
• /
• 2023

Messenger RNA (mRNA)-based vaccines and treatments have recently emerged as a promising strategy. Naked mRNA presents various limitations for direct delivery. Therefore, in this paper, Lipid Nanoparticles (LNPs) were utilized for the delivery of mRNA. Lipid nanoparticle (LNP) mRNA systems are highly effective as vaccines, but their efficacy for pulmonary delivery has not yet been fully established. Additionally, research on effective delivery systems and administration methods for vaccines is required to resolve the stability and degradation issues associated with naked mRNA delivery. This study aimed to determine mRNA delivery efficiency via the inhalation of a lipid nanoparticle (LNP) formulation designed specifically for pulmonary delivery. To this purpose, we built a library of seven LNP configurations with different lipid molar and N/P ratios and evaluated their encapsulation efficiency using gel retardation assay. Among the tested LNPs, LNP1, LNP2-2, and LNP3-2 demonstrated high transfection efficiency in vitro based on FACS analyses luciferase assays, and intracellular accumulation tests. The mRNA delivery efficiencies of the selected LNPs after inhalation and intravenous injection were compared and evaluated. LNP2-2 showed the highest mRNA expression in healthy mouse lungs when aerosolized and was found to be non-toxic. These results indicate that LNP2-2 is a promising carrier for lung mRNA delivery via inhalation.

• 분석과학
• /
• 제26권1호
• /
• pp.61-66
• /
• 2013

얇은 유리판 위에 잘 정렬된 인지질 이중막에 작용하는 항균성 펩타이드 protegrin-1의 활성작용을 고체 $^2H$ 핵자기 공명 분광법을 이용하여 조사하였다. 수화 전후에 있어서 인지질 분자의 배향분포와 그 운동성을 고체 $^2H$ 핵자기 공명 스펙트럼을 통하여 확인하였다. 진공으로 건조된 상태에서는 인지질 분자의 운동성이 작다는 사실 외에는 인지질 분자들의 배향분포에 대한 정확한 상태를 알 수 없었다. 그러나 시료가 수화된 이후에는 시간이 지남에 따라 인지질 분자들이 잘 정렬되어 간다는 것과 원환체 기공이 형성되어 간다는 것을 확인할 수 있었다. Protegrin-1과 같은 항균성 펩타이드에 의한 인지질 이중막 변화를 본 연구논문에서 사용한 방법으로 확인하기 위해서는 상당히 긴 반응시간이 필요하다는 것을 확인하였다.

• Journal of Nutrition and Health
• /
• 제40권8호
• /
• pp.693-700
• /
• 2007

This study was performed to investigate the effects of genistein, a kind of soy isoflavones, on fatty liver and lipid metabolism in rats fed high fat diet. Twenty four male Sprague-Dawley rats were divided into four groups by dietary fat and genistein contents then raised for six weeks. The rats(n=6/group) were fed normal fat diet(NOR), high fat diet (HF), high fat with 0.1% genistein(HF+0.1%G) or high fat with 0.2% genistein(HF+0.2%G). Hepatic total lipid, triglyceride, total cholesterol and Serum GPT, as a marker for fatty liver, were significantly increased by high fat diet. Also, serum total lipid, triglyceride, total cholesterol, glucose and insulin concentration, hepatic lipogenic enzyme (fatty acid synthase and malic enzyme) activities were significantly increased by high fat diet. However, hepatic total lipid, triglyceride, total cholesterol and Serum GPT were significantly decreased by genistein intake. Also, genistein supplementation decreased serum total lipid, triglyceride, glucose and insulin concentration, hepatic lipogenic enzyme (fatty acid synthase and malic enzyme) activities. There were no differences by genistein level except for serum insulin. These results suggest that fatty liver induced by high fat diet was caused by increased serum lipid profiles and hepatic lipogenesis, whereas, genistein may be useful in inhibiting of fatty liver by reducing serum lipid profiles and hepatic lipogenesis.

• 한국식품영양과학회지
• /
• 제37권4호
• /
• pp.422-427
• /
• 2008

자두와인이 지질대사 및 지질과산화에 미치는 영향을 측정한 결과는 다음과 같다. 4주간의 식이공급 후 알코올대조군과 저알코올 자두와인의 식이효율은 정상대조군에 비해 감소하지 않았으나 자두와인의 식이효율은 감소하였다. 저알코올 자두와인의 체중 100 g당 간무게, 혈장 내 총콜레스테롤 및 동맥경화지수가 알코올대조군에 비해 유의하게 낮았으며 총콜레스테롤에 대한 HDL-콜레스테롤의 비는 알코올대조군에 비해 유의하게 증가하였다. 저알코올 자두와인과 자두와인의 간조직 중 총지질, 중성지질, 총콜레스테롤 및 혈장 AST, ALT 활성은 알코올대조군에 비해 유의하게 낮았다. 혈장 및 간조직의 지질과산화물 수준은 저알코올 자두와인이 알코올대조군에 비해 유의하게 낮았으며 자두와인의 혈장 및 간조직의 지질과산화물 수준은 저알코올 자두와인보다는 높고 알코올대조군보다는 낮은 수준이었다. 본 연구의 결과, 자두와인을 적정량 음용할 경우 순수한 알코올을 음용하는 것에 비해 혈장 및 간의 지질대사에 긍정적인 영향을 주며 혈장 및 조직의 지질과산화물 생성을 억제하는 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제25권3호
• /
• pp.369-374
• /
• 2012

A $3{\times}2$ factorial experiment was conducted to determine the effects of dietary protein and lipid levels on the growth and body composition of juvenile far eastern catfish. Six diets were formulated to contain three levels of protein (20%, 30% and 40%) and two levels of lipid (9% and 17%). Triplicate groups of fish (initial body weight of 7.6 g) were hand-fed to apparent satiation for 66 days. Final mean weight was improved with increasing dietary protein and lipid levels, and the highest final mean weight was observed in fish fed the 40/17 (% protein/% lipid) diet. No significant difference was observed in final mean weight for fish fed between 30/17 diet and 40/9 diet. Feed efficiency of fish fed the diets containing over 30% protein levels with 9% and 17% lipid levels were significantly higher than those of fish fed the 20% protein levels. Feed efficiency of fish fed the 30/17 diet was not significantly different from that of fish fed the 40/9 diet or 40/17 diet. Feed efficiency and protein efficiency ratio of fish fed the 20% protein diets with 17% lipid level were significantly higher than those of fish fed 9% lipid diet. Daily feed intake of fish tended to decrease with increasing dietary protein and lipid levels. Moisture content of whole body in fish fed the 9% lipid diets was significantly higher than that of fish fed the 17% lipid diets at the same protein level, but the opposite trends were found for crude lipid content. Significant effects of dietary lipid were observed for most fatty acids, according to their relative values in the diets. The results of this study suggest that the protein requirement for maximum growth of juvenile far eastern catfish may be higher than 40%, and an increase of dietary lipid level from 9% to 17% can improve growth and feed utilization.

• 생명과학회지
• /
• 제8권3호
• /
• pp.305-311
• /
• 1998

These studies were undertaken to investigate changes of neutral lipid content and fatty acid composition were determined. Also accumulation process of monoglyceride, diglyceride and triglyceride content, and fatty acid composition were investigated during the development. The results were summarized as follows ; Changes of lipid during development sesame seeds, glycolipid contents which showed the highest in the early ripening stage and after that rapidly decreased, and phospholipid contents showed a similar pattern as glycolipid occurred. In contrast, the content of neutral lipid was rapidly increased by 29.21% 10 days after flowering(DAF), and showed the highest value by 91.84% at 40th day after flower. The neutral lipid, triglyceride content was rapidly increased as the seeds developed, and consisted of over 60% of the neutral lipid since 30 DAF. In the changes of neutral lipid, phospholipid and glycolipid, stearic acid and palmitic acid decreased during the seed ripening. However, oleic acid and linoleic acid increased during the same periods. Linolenic acid, which showed relatively higher value in the early ripening stage, but rapidly decreased as much as 1% at the later ripening stage.

• Journal of Nutrition and Health
• /
• 제31권7호
• /
• pp.1112-1120
• /
• 1998

This study was conducted to investigate the effets of chitosan and beef tallow at different level on glucose and lipid metabolism in rats. Dietary fot level was 20% and 40%, and chitosan was given at levels of 0%, 3%, and 5%(wt/wt) of diet. Chitosan supplement tended to decrease the serum total lipids, total cholesterol, and triglycerides. HDL cholesterol and HDL cholesterol : total cholesterol ratio tended to increase with 5% chitosan supplementation. LDL cholesterol and VLDL triglyceride tended to decrease with chitosan supplementation. Lipid concentration of liver and epididymal fat pad(EEP) tended to decrease with medium dietary fat and chitosan treatment. fecal excretion of total lipid and triglyceride exhibited a tendency to increase with high fat levels and chitosan. Length of small intestine and gastrointestinal transit time were not affected by dietary fit levels or chitosan supplements. Therefore, it could be suggested that chitosan supplement had beneficial effects on lipid metabolism. (Korean J Nutrition 31(7) : 1112-1120, 1998)

• 한국환경보건학회지
• /
• 제23권3호
• /
• pp.66-71
• /
• 1997

To investigate the effect of several factors on serum lipids in maternal and infants umbilical cord blood, 111 cases of newborn infants and women delivered of a child who had delivered at hospital located in Chung-Ju city from Feb. 1, 1996 to Aug. 31, 1996 were studied. The gestational ages of cases were 37 to 42 weeks and the average maternal ages were 28.1$\pm$4.25 years old without any other medical or obstetric problems. No relationships between maternal consumption of coffee and maternal serum lipid levels were observed. However, serum total cholesterol and LDL-cholesterol levels in infants'umbilical cord were significantly increased in proportion to increasing maternal consumption of coffee. The other factors such as alcohol drinking habits and residency affected maternal serum lipid levels and not serum lipid levels in infants' umbilical cord. Delivery frequency showed very little or no effects on serum lipid levels in both maternal blood and infant's umbilical cord blood. In conclusion, maternal consumption of coffee significantly correlated with increasement of serum lipid levels in infants'umbilical cord.

• Archives of Pharmacal Research
• /
• 제17권2호
• /
• pp.109-114
• /
• 1994

The role of sex homones in hepatic lipid peroxidation, and in hepatic adehyde odidase and xanthine oxidase activites were investigated using rat liver homogenates. It was observed that male rt had a significantly greater content of malondialdehyde in liver than female. Among the sex hormones tested, estradiol, one of female hormones, markedly inhibited the formation of lipid peroxides in liver tissues in vitro. Especially, the inhibitory effect of estradiol appeared more remarkably in Fe-induced lipid peroxidation. The hepatic xanthine oxidase activity was decreased about 15% by $10\;^6\;M$ estradiol, wherease, the adehyde oxidase activity was almost completely disappeared at the same concentration of estradiol. It implies that sex differences in lipid peroxidation is attributed to the suppression of radical generating system by estradiol.

• 한국응용과학기술학회지
• /
• 제33권4호
• /
• pp.667-675
• /
• 2016

Nanoemulsions are actively used in several applications for pharmaceutical, cosmetic and chemical industries. In this study, we propose the use of microfluidizer known as high pressure homogenizer to prepare lipid nanoemulsion as a potent cosmetic delivery carrier. The lipid nanoemulsions were prepared by O/W emulsion with hydrogenated lecithin and different type of oils. Effects of oil type on the stability of the lipid nanoemulsion were investigated with Dynamic Light Scattering (DLS) and Zeta-potential. Arbutin was used as model drug for transdermal administration through hairless mouse skin. Transdermal arbutin delivery using the lipid nanoemulsions was studied with HPLC method.