• Mycobiology
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• 제51권1호
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• pp.60-66
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• 2023

In this study, the α-amylase inhibitory activity, α-glucosidase inhibitory activity, pancreatic lipase inhibitory activity, and Xanthine Oxidase inhibitory activity of the fruiting body extracts of 5 varieties of Agaricus bisporus (AB) were confirmed. First, the α-amylase inhibitory activity of AB12, AB13, AB18, AB34, and AB40 methanol extracts was lower than that of acarbose, a positive control, in all concentration ranges. The α-glucosidase inhibitory activity of the AB40, AB13, and AB12 methanol extracts at the extract concentration of 1.0 mg/mL was 80.5%, 81.3%, and 78.5%, respectively, similar to that of acarbose, a positive control. The pancreatic lipase inhibitory activity of the methanol extract of Agaricus bisporus fruiting body was significantly lower than that of the positive control orlistat in the concentration range of 50~1.000 (mg/mL). The Xanthine Oxidase inhibitory activity was 0.5~8.0 mg/mL of each extract, which was significantly lower than that of the positive control allopurinol in the same concentration range. However, the Xanthine Oxidase inhibitory activity of AB13 and AB40 at 8.0 mg/mL was about 70%, which was higher than that of other mushrooms. In conclusion, five kinds of Agaricus bisporus fruiting bodies seem to have inhibitory effects on enzymes such as α-amylase, α-glucosidase, pancreatic lipase, and Xanthine Oxidase that degrade starch and protein. In particular, it has an inhibitory effect and a reduction effect on xanthine oxidase that causes gout, so it is expected that it can be developed and used as a food or health supplement with health functional properties through future research.

• Journal of Plant Biology
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• 제33권4호
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• pp.217-223
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• 1990

Restriction enzyme inhibiting substance (REIS) extracted from spinach chloroplast and Chlamydomonas seems not to be proteinaceous, because its inhibiting activity was not lost by heat or trypsin treatment. And it seems not to be lipid or polysacchride, because its inhibiting activity was not lost by lipase or $\alpha$-amylase treatment, respectively. In Chlamydomonas, putrescine, spermidine and spermine were present. The amount of putrescine was the smallest and that of spermie was the greatest. But only spermine was contained in REIS and the activity of REIS. It was proportional to the amount of spermine in REIS and it was hindered by Na+ ion. So, the inhibiting activity of REIS seems to be deeply related to spermine contained in REIS. But restriction enzyme inhibiting activity remained to the some extent although salts and spermine were eliminated by dialysis.

• Archives of Pharmacal Research
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• 제28권2호
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• pp.180-185
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• 2005

In the process of investigating anti-obesity effect of Platycodi Radix, we found that aqueous extract of Platycodi Radix might inhibit intestinal absorption of dietary fat by inhibiting pancreatic lipase (PL) activity. In order to clarify the anti-obesity mechanism of Platycodi Radix, activity-guided isolation was performed to find active components. The total saponin fraction of Platycodi Radix appeared to have a potent inhibitory activity against the hydrolysis of triolein emulsified with phosphatidycholine by pancreatic lipase in vitro. Based on these results, further purification of active components yielded 10 known triterpenoidal saponins, among these compounds, platycodin A, C, D, and deapioplatycodin D exhibited significant inhibitory effects on PL at the concentration of $500\;{\mu}g/mL$ with 3.3, 5.2, 34.8, and $11.67\%$ pancreatic lipase activity vs control, respectively. Platycodin D was found to inhibit the PL activity in a dose-dependent manner. Therefore, the anti-obesity effect of Platycodi Radix might be due to the inhibition of pancreatic lipase by its saponins.

• Journal of Applied Biological Chemistry
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• 제60권3호
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• pp.241-244
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• 2017

제주 동백나무 겨우살이 추출물의 항산화 활성을 DPPH 라디컬 소거능, 철이온 킬레이팅과 환원력 시험을 통해 조사한 결과, 메탄올과 에탄올 추출물이 열수 추출물보다 뛰어난 항산화 효능을 보였다. ${\alpha}$-Glucosidase와 pancreatic lipase 저해능 또한 메탄올과 에탄올 추출물이 우수한 활성을 보였다. 따라서, 제주 동백 나무 겨우살이의 메탄올 및 에탄올 추출물을 건강기능 의약식품 소재로 개발하기 위한 추가 연구가 진행되어야 할 것이다.

• 한국식품영양과학회지
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• 제40권2호
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• pp.308-315
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• 2011

연구는 강원도 자생 산채류의 다양한 약리 기능 중 ${\alpha}$-glucosidase, ${\alpha}$-amylase, lipase 효소 저해활성이 우수한 식물소재를 탐색하는데 있다. 강원도 양구, 평창, 태백에서 자생하고 있는 산채 90종을 채집하여 물과 에탄올로 각각 추출하였다. 항비만 활성 탐색은 ${\alpha}$-amylase, ${\alpha}$-glucosidase, lipase 저해활성을 검정하였다. 그 결과 산채 90종 중 ${\alpha}$-amylase 활성 저해능이 높은 식물로 에탄올 추출물 3종, 물추출물 2종, ${\alpha}$-glucosidase 활성 저해능이 높은 식물로 에탄올 추출물 13종, 물 추출물 3종, lipase 활성 저해능이 높은 식물로 에탄올 추출물 7종, 물 추출물 1종이 선발되었다. 향후 본 연구결과는 비만과 당뇨 등 대사성 질환에 대해 예방 혹은 치료 효과가 있는 기능성식품으로 개발하는데 있어 좋은 기초자료로 활용될 것으로 기대된다.

• 대한본초학회지
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• 제32권4호
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• pp.9-15
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• 2017

Objectives : In this study, the inhibitory activities of Korean Medicine against pancreatic lipase in vitro and biochemical analyses in vivo were measured to determine its possibility as a well-matched sauce material with chicken. Methods : The inhibitory activity on pancreatic lipase enzyme of 11 samples were evaluated in vitro and then 5 samples were selected. The activity of pancreatic lipase was investigated using orlistat as a positive control. Animals were divided into eight groups (n=7). The experimental groups except for normal group were fed 60% high-fat diet for 7 days. 5 samples were orally administered at a dose of 200 mg/kg body weight and orlistat were orally administrated at a dose of 60 mg/kg body weight for 7 days. Biochemical anaylses of 5 samples were executed based on lipid parameters analysis. Results : Korean Medicines with an $IC_{50}$ of below 1 mg/kg were Scutellariae Radix, Gardeniae Fructus, Theae Folium Coptidis Radix, and Mori Cortex Radicis. Body weight change of Mori Cortex Radicis reduced significantly, however fecal triglyceride couldn't regulate effectively. The most excellent inhibitory effect of pancreatic lipase showed in Scutellariae Radix treatment and also regulated significantly serum triglyceride and total cholesterol. Moreover, the supplementation of Coptidis Radix excreted meaningfully triglyceride to fece. Conclusions : In conclusion, Coptidis Radix may exert anti-obesity effect by directly inhibiting pancreatic lipase, which would prevent the absorption of lipid from the small intestine. Besides, Mori Cortex Radicis may led to the decrease of the body weight via the different pathway.

• 한국식품영양과학회지
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• 제42권4호
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• pp.556-562
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• 2013

본 연구에서는 기존의 유기용매를 이용한 추출을 지양하고 환경 친화적이고 간단한 추출방법을 고안하여 톳의 다당류 성분(WSP-A)을 추출해내었고, WSP-A가 지질의 흡수에 미치는 효과를 알아보기 위하여 in vitro 상으로 lipase inhibition assay와 in vivo로 고지방 식이로 비만을 유도한 rat을 이용한 동물실험을 수행하였다. 그 결과 톳 유래 다당류는 alginate와 유사한 성분이지만 성분당 분석을 통하여 fucose가 주 당성분인 polysaccharide로 밝혀졌고, 이는 alginate보다 뛰어나고 안정한 lipase inhibitor로써 작용하였다. 또한 동물실험에서는 WSP-A를 포함하는 음용수로 제공한 것과 제공하지 않은 대조군은 무게 변화량에 유의적인 차이를 보일 뿐 아니라 비만 예방군과 비만 치료군 사이에서도 유의적인 차이를 나타내었다. 식이섬유를 이용한 연구와 비교해보았을 때, 톳 유래 다당류는 식이섬유 이상의 효과뿐만 아니라 뛰어난 lipase inhibitor로 체중감량을 나타내었고 이는 인간췌장유래 리파아제(human pancreatic lipase)의 저해활성과 더불어 인체 내에서의 작용 가능성을 짐작하고 있다. 따라서 톳 유래 다당류 성분은 식이성 섬유의 공급원 뿐만 아니라 추후에 효소활성 저해 효과에 대한 보다 명확한 작용기전을 밝혀낸다면 단순한 건강 기능성 식품 소재보다 lipase inhibitor로써 항비만 약물로 고부가가치를 창출해낼 수 있을 것으로 사료된다.

• 생약학회지
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• 제41권3호
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• pp.216-220
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• 2010

Obesity is an important risk factor that significantly increases mortality and disease rates in the cardiovascular disease, diabetes, and various diseases. So far, the most powerful way to inhibit fat absorption is pancreatic lipase inhibitors. In this study, we investigated the anti-obesity effect of the extract of Juniperus rigida. Juniperus rigida extract (JRE) had a inhibitory effect on pancreatic lipase activity ($IC_{50}$=8.63 ${\mu}g$/ml). In in vivo oil-emulsion loading test, this extract also inhibited the intestinal fat absorption. In addition, we measured inhibitory effects of JRE on activity of phosphodiesterase (PDE) and hormone sensitive lipase (HSL) among the important enzymes associated with lipolysis. JRE strongly inhibited PDE activity ($IC_{50}$=4.56 ${\mu}g$/ml), whereas inhibitory effect on HSL activity was very weak compared with orlistat. As a result, JRE inhibited the absorption of fat by inhibiting the activity of pancreatic lipase and induced lipolysis through inhibition of PDE activity. Therefore, we suggest that Juniperus rigida may be a potential therapeutic agent improving obesity.

• 한방재활의학과학회지
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• 제30권4호
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• pp.41-53
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• 2020

Objectives The purpose of this study was to investigate the effects of Silbi-san on the antioxidant and fat accumulation inhibition and to analyze the anti-obesity effect by analyzing the changes in serum lipid composition in obese mice. Methods We compared contents of phytochemicals like total polyphenols and total flavonoid and antioxidant activities such as 2,2-dipheny-1-picrylhydrazyl and 2.2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity. After Silbi-san in 3T3-L1 cells in vitro and mouse adipose tissue ex vivo, we quantified intracellular triglyceride accumulation and lipolysis. Moreover, the anti-obesity activity though inhibiting pancreatic lipase were analyzed. In 3T3-L1 cells, morphological changes showed that control cells had many lipid while cells treated with Silbi-san had less lipid accumulation. 30% EtOH Silbi-san treatment also suppressed the fat absorption by inhibiting the activity of pancreatic lipase and led to high lipolysis through promoting glycerol release. The experimental group was divided into four groups: Normal group fed normal feed, Control group fed 60% high fat diet (HFD) and distilled water, drug group fed 60% high fat diet and 200 mg/kg of Silbi-san water extract, drug group fed 60% HFD and 200 mg/kg of Silbi-san 30% ethanol extract. Results Serum total cholesterol content and serum low density lipoprotein-cholesterol content were significantly decreased in the Silbi-san extract group compared to the control group, serum high density lipoprotein-cholesterol content was significantly increased in Silbi-san extract group. Conclusions In this study, the antioxidant and fat accumulation inhibitory effects of Silbi-san were confirmed.

• 대한본초학회지
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• 제38권3호
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• pp.1-10
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• 2023

Objective : Obesity in modern society has a significant impact on pathological, psychological and social problems. Therefore, many studies on obesity treatment using herbal extracts with fewer side effects have been conducted. This study was designed to investigate the effect of inhibiting fat accumulation in vitro in order to Mongolian medicinal plants find anti-obesity candidate among. Methods : We measured pancreatic lipase inhibitory activity in Mongolian medicinal plants extract. Cytotoxicity of these extracts was monitored in 3T3-L1 cells by MTT assay. In addition, the anti-obesity effects was confirmed at concentrations of 0.2, 0.1 and 0.05 mg/㎖ through Oil red O staining. Results : Among Mongolian medicinal plants, Rheum undulatum roots in September (RURS), Paeonia anomala L. (PAL), and Fragaria orientalis (FO) showed the highest pancreatic lipase inhibitory activity. As a result of the MTT assay, more than 80% was judged to be non-toxic, and the concentration was determined, and as a result of evaluating the lipid accumulation inhibitory effect, 6 types were selected as candidates. Conclusion : Based on these results, the top 7 species expected to be used as anti-obesity functional materials were selected. However, additional efficacy verification and mechanism of action need to be established in the future. So, it is expected that the medicinal plants verified through this will be used as functional materials for the prevention and treatment of obesity.