• YAKHAK HOEJI
• /
• v.50 no.2
• /
• pp.78-83
• /
• 2006

VISA and VRE are the main causes of surgical infection, urinary tract infections and bacteremia in hospitals. In this study; we selected VISA (Vancomycin Intermediate resistant Staphylococcus aureus) and VRE (Vancomycin Resistant Enterococcus) isolated from the clinical isolates. One of the isolated strains indicated the high resistance to severel anti-biotics (Vancomycin, Teicoplanin, Mupirocin, Synercid, Ciprofloxacin, Gentamicin, Lincomycin, Cefotaxim, Meropenem). Antimicrobial activity of Bifidobacterium spp. against VISA and VRE were measured. About $10^4$ cells of VISA or VRE were mixed with 1,5 and 9 ml of Bifidobacterium and the final volume was adjusted to 10 ml with brain heart infusion (BHI) broth. The cell suspension was incubated for 3, 6, 9, and 24 hr, serially diluted and then plated on BHI agar plate. As numbers of Bifidobacterium were increased viable cell count of VISA and VRE decreased. The strongest antimicrobial activity of the Bifidobacterium was observed after 9hr incubation in any mixture, almost completely inhibiting the growth of VISA and VRE.

• Korean Journal of Veterinary Service
• /
• v.23 no.1
• /
• pp.77-91
• /
• 2000

This study was conducted to investigate the antibiotic resistance and plasmid profiles of 58 salmonella spp isolated from mesenteric lymphnodes of slaughter pigs in Kyoungbuk province during the period from September 1997 to June 1998. The results obtained are as follow that all isolates were susceptible to amikacin, gentamicin, ciprofloxacin and enrofloxacin, and the majority of isolates were highly susceptible to norfloxacin, colistin, nalidixic acid and apramycin while they were moderately susceptible to kanamycin, cephalothin, chloramphenicol, ampicillin, trimethoprim and penicillin. The majority of isolates were over 90% resistant rates to lincomycin, sulfadimethoxine, vancomycin, methicillin and erythromycin. The plasmid profiles of 58 salmonella spp are developed 1 to 4 fractions, 0.9 to 29.5 Kb molecular range sizes and U strains (45.5%) were showed plasmid profiles by agarose gel electrophoresis. 5 derby harbored 29.5 Kb and 7 Kb, and S schwarzengrund had 14 Kb and 0.9 Kb harboring sizes. Four of 10 S agona and 2 of 4 S typhimurium were harbored 3.1 Kb and n.5 Kb, respectively. Thirty-five untypable strains are developed variable size fractions its showed small size plasmid profile less than 6 Kb and 22 (62.8%) of them had no detectable plasmids.

• Journal of Korean Society of Water and Wastewater
• /
• v.26 no.3
• /
• pp.373-381
• /
• 2012

Antibiotics have been issued recently in water environments because of potential impacts on ecosystem and public health. This study was aimed to investigating the degradation of antibiotics such as tetracycline, lincomycin, sulfamethazine and cephradine using gamma ray irradiation. And the toxicity before and after irradiation on antibiotics was tested in order to examine the aquatic eco-risk assessment by aquatic organisms. In addition, comparing tests on toxicity for gamma ray and UV irradiated antibiotics was conducted. Four different antibiotics were prepared by concentration of 30 mg/L with demi-water respectively. The absorption dose of gamma ray was ranged from 0.2 to 2 kGy. The concentration of four antibiotics was gradually decreased corresponding to the increase of the absorption dose. A method for toxicity assessment using Pseudokirchneriella subcapitata was evaluated to the most acceptable compared with methods by Daphnia magna and Microtox$^{(R)}$ in terms of sensibility. It showed that the reduction of toxicity on antibiotics treated by gamma ray was superior comparing to the test results obtained from UV treatment. By-products from antibiotics treated by gamma ray were easily decomposed by microorganism and their toxicity was also evaluated to low.

• Korean Journal of Veterinary Service
• /
• v.18 no.1
• /
• pp.22-32
• /
• 1995

A partial characteristics and pathogenicity of Aeromonas salmonicida strain isolated from diseased cultured eel were studied. On the biochemicial characteristics of the isolate strain was classified into atypical Aeromonas salmonicida : the characteristics of the isolate strain differed from those of the subspecies of Achromogenes, masoucida and salmonicida. It strain was named atypical Aeromonas salmonicida EL-1 and it grew at optimal pH 7.5 and l% Nacl. In the pathogenicity test, at one to two days post infection, the motality of artificially infected eels with $1{\times}10^8$ and $1.0{\times}10^7$ cfu/fish group was 100%, and at three days pos-infection, in the $1.0{\times}10^6$ /cfu /fish infected group, the epizootic haemorrhagic ulcer was developed, and haemorragic red sports were formed around abdomen and fins and its died (40%). Total cell protein peptide bands of the A. salmonicida EL-1 were presented betweeon 11.8~102 Kd in molecular weight by the electrophoretic analysis and appeared 28 bands in all. Drug sensitivity of atypical A. salmonicida EL-1 strain was sensitivity to chlorampenicol, gentamicin, kanamycin, streptomycin and sulfamethoxazole /trimethoprim and intermidiate to ampiclin and neomycin resistant to cephalotin, erythromycin, lincomycin and tetracycline.

• Korean Journal of Veterinary Service
• /
• v.42 no.4
• /
• pp.301-304
• /
• 2019

The objective of this study was to determine the in vitro intracellular and extracellular minimum inhibitory concentrations (MICs) of 13 antimicrobials against one recently isolate Lawsonia intracellularis, the etiological agent of proliferative enteropathy (PE). The final MICs were assessed by counting the number of heavily infected cells (HICs;>30 bacteria per cell) using an immunoperoxidase monolayer assay. Enrofloxacin (InMIC; 1~2 ㎍/mL and ExMIC; 16 ㎍/mL) still presented the most notable antimicrobial susceptibility, and marbofloxacin (2 ㎍/mL and 8 ㎍/mL) was followed. Colistin (0.25 ㎍/mL and 2 ㎍/mL) presented a susceptibility followed by tylvalosin (1 ㎍/mL and 2 ㎍/mL). Florfenicol and lincomycin had the weakest susceptibility and amoxicillin, penicillin G, chlortetracycline, oxytetracycline, tiamulin, tilmicosin, and tylosin displayed weak susceptibility. Although some antibiotics showed decreased susceptibility patterns, they showed similar patterns to recent antibiotic susceptibility patterns in Korea. In addition, these results could be one of contributions in clinical fields.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.25 no.1
• /
• pp.143-149
• /
• 1996

균주개량 방법의 일환으로 electrofusion 법을 이용하여 protease 화성, lipase 활성, 내열성, 내산성 등이 우수한 L. bulgaricus와 bacteriocin을 생산하는 L. acidophilus 간의 융합조건을 검토하였다. Electrofusion에 의해 생성된 융합주는 식별하기 위해 streptomycin(2mg/ml)과 lincomycin(5$\mu\textrm{g}$/ml)에 내성을 나타내는 L. bulgaricus 변이주이와 kanamucin (600$\mu\textrm{g}$/ml)에 내성을 나타내는 L. acidophilus 88 변이주를 분리하였다. Electrofusion을 500V/cm, 100mses(49phms, 2300 capacotance)에서 수행했을 때 가장 융합이 잘 일어났으며 전기장의 세시와 시간이 증가할수록 융합요율이 현저히 감소하였다. 2가 양이온은 대체적으로 융합효율을 감속시키는 경향을 나타내었으나 1mM MgCl$_2$에서 융합 효율이 약간 증가하였다. PEG 400을 elec-trogusion buffer에 30%되도록 첨가했을 때, 가장 양호한 융합효율을 나타내었으며 그 이상에서는 오히려 융합 효율이 감소하였다. Electrofusion법과 PEG 매개에 의한 융합법의 융합 효율을 비교한 결과, 거의 비슷한 융합 효율을 나타내었으나 electrofusion 법과 PEG매개에 의한 융합법을 혼합하였을 때 가장 양호한 융합 효율을 나타내었다.

• Journal of Veterinary Clinics
• /
• v.3 no.1
• /
• pp.243-253
• /
• 1986

In recent years much attention has been paid to swine respiratory infection caused by Haemophilus(H.) Pleuropneumoniae with rapid expansion of pork industry in Korea. The organism may cause an acute respiratory infection with high morbidity and mortality, or it may also cause chronic persistent infection, resulting in loss of body weight. The present study was performed to observe the etiologic situation of H. Pleuropneumoniae infection by evaluating bacteriological, serological examinations with the lungs and sera collected from slaughtered pigs in Daegu city, respectively. In addition, antibiotic susceptibility test was carried out against the isolated strains of H. Pleuropneumoniae. The results obtained are summarized as follows : 1. Only 10 strains of H. Pleuropneumoniae were isolated from the 120 cases of swine lungs examined, and biological and biochemical characteristics of the isolates were identical with that of standard strains(serovar 1 to 6). 2. Out of 336 swine sera examined, 108(32.1%) were shown to be positive against H. Pleuropneumoniae by latex agglutination test. 3. Antibiotic susceptibility test revealed that 10 isolates of H. Pleuropneumoniae were sensitive to 16 kinds of antibiotic such as ampicillin, benzylpenicillin, piperacillin, cephalothin, cefazolin, cefamandol, cefoperagon, lactomoxycefa, tetracycline, minocycline, chloramphenicol, gentamicin, kanamycin, amikacin, colistin and nalidixic acid, whereas cloxacillin, erythromycin, kitasamycin, lincomycin, oleand-amycin, and bacitracin were resistent.

• Korean Journal of Veterinary Service
• /
• v.37 no.3
• /
• pp.165-171
• /
• 2014

This study was conducted to investigate the species-specific gene detection, capsular serogroup and antimicrobial resistance pattern of Pasteurella multocida isolated from pneumonic lung lesion of swine in Gyeongbuk province. P. multocida isolates were typed for capsular serogroups by polymerase chain reaction. Of the 32 strains, 28 (87.5%) were typed serotype A, 3 (9.3%) were typed serotype D, and 1 strain was unknown (3.1%), respectively. In antimicrobial agents resistance test, almost of strains were susceptible to amoxicillin (100%), enrofloxacin (96.9%), ampicillin (93.8%), florfenicol (90.6%), chloramphenicol (90.6%) and were resistant to streptomycin (71.9%), spectinomycin (56.3%). All strains were resistant to clindamycin, erythromycin and lincomycin.

• Korean Journal of Veterinary Research
• /
• v.39 no.1
• /
• pp.96-103
• /
• 1999

The present study was carried out to investigate the prevalence of mycoplasmal pneumonia of slaugter pigs in Youngnam area during the period from 1995 to 1997. The prevalence and pathomorphology of gross lung lesions were studied from 682 slaughter pigs in 8 swine herds. Gross lesions of pneumonia were recorded in the lungs of 442(64.8%), from 367 out of them(83.0%) were diagnosed as mycoplasmal pneumonia. Microbiological examination was performed with 197 lungs with gross lesions of mycoplasmal pneumonia of slaughter pigs from 8 different swine herds. M hyopneumoniae, P multocida, A pleuropneumoniae, Streptococcus spp, Corynebacterium spp, and H parasuis were detected in 24. 4%, 48.2%, 2.5%, 11.2%, 3.6%, and 1.0% of the pneumonic lungs, respectively. A total of 48 strains of M hyopneumoniae was investigated for their in vitro susceptibility to 8 antibiotics. Among the drugs tested, lincomycin, oxytetracycline, tiamulin and tylosin showed the high activity in minimal inhibitory concentration(MIC) of $0.04{\sim}5{\mu}g/ml$ while erythromycin showed low activity in MIC values($1.25{\sim}{\geq}40{\mu}g/ml$).

• Journal of Food Hygiene and Safety
• /
• v.22 no.2
• /
• pp.116-119
• /
• 2007

The antimicrobial susceptibility and antibiotic resistance patterns of 67 eae positive Escherichia coli strains isolated from pigs were investigated by disc diffusion method. Sixty-seven E. coli isolated from pigs showed susceptibility to Ceftiofur (98.5%), Lincomycin+Spectinomycin (74.6%), Danofloxacin (73.1%), Enrofloxacin (64.2%), and Neomycin (41.8%). However, the multiple resistance patterns were also seen in eae+E. coli isolates. Neomycin+Tylosin+Penicillin+Tetracycline, Tylosin+Penicillin+Tetracycline, and Neomycin+Tylosin+Danofloxacin+Penicillin+Tetracycline+Enrofloxacinwere the most prevalent patterns of multiple antibiotic resistance.