• Title/Summary/Keyword: limited measurements

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A Bio-fluidic Device for Adaptive Sample Pretreatment and Its Application to Measurements of Escherichia coli Concentrations

  • Choi Won-Jae;Park Je-Kyun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.1
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    • pp.54-60
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    • 2006
  • In this paper, we describe a bio-fluidic device for adaptive sample pretreatment, in order to optimize the conditions under which absorbance assays can be conducted. This device can be successfully applied to the measurement of Escherichia coli (E. coli) concentrations using adaptive dilution, with which the dilution ratio can be adjusted during the dilution. Although many attempts have been previously made to miniaturize complex biochemical analyses at the chip scale, very few sample pretreatment processes have actually been miniaturized or automated at this point. Due to the lack of currently available on-chip pretreatments, analytical instruments tend to suffer from a limited range of analysis. This occasionally hinders the direct and quantitative analysis of specific analyses obtained from real samples. In order to overcome these issues, we exploit two novel strategies: dilution with a programmable ratio, and to-and-fro mixing. The bio-fluidic device consists of a rectangular chamber constructed of poly(dimethylsiloxane) (PDMS). This chamber has four openings, an inlet, an outlet, an air control, and an air vent. Each of the dilution cycles is comprised of four steps: detection, liquid drain, buffer injection, and to-and-fro mixing. When using adaptive sample pretreatment, the range in which E. coli concentrations can be measured is broadened, to an optical density (O.D.) range of $0.3{\sim}30$. This device may prove useful in the on-line monitoring of cell concentrations, in both fermenter and aqueous environments.

The effects of different polishing techniques on the staining resistance of CAD/CAM resin-ceramics

  • Sagsoz, Omer;Demirci, Tevfik;Demirci, Gamze;Sagsoz, Nurdan Polat;Yildiz, Mehmet
    • The Journal of Advanced Prosthodontics
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    • v.8 no.6
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    • pp.417-422
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    • 2016
  • PURPOSE. The purposes of this study were to evaluate the staining resistance of CAD/CAM resin-ceramics polished with different techniques and to determine the effectiveness of the polishing techniques on resin-ceramics, comparing it with that of a glazed glass-ceramic. MATERIALS AND METHODS. Four different CAD/CAM ceramics (feldspathic ceramic: C-CEREC Blocs, (SIRONA) and three resin-ceramics: L-Lava Ultimate, (3M ESPE), E-Enamic, (VITA) and CS-CeraSmart, (GC)) and one light cure composite resin: ME-Clearfil Majesty Esthetic (Kuraray) were used. Only C samples were glazed (gl). Other restorations were divided into four groups according to the polishing technique: nonpolished control group (c), a group polished with light cure liquid polish (Biscover LV BISCO) (bb), a group polished with ceramic polishing kit (Diapol, EVE) (cd), and a group polished with composite polishing kit (Clearfil Twist Dia, Kuraray) (kc). Glazed C samples and the polished samples were further divided into four subgroups and immersed into different solutions: distilled water, tea, coffee, and fermented black carrot juice. Eight samples ($8{\times}8{\times}1mm$) were prepared for each subgroup. According to CIELab system, four color measurements were made: before immersion, immersion after 1 day, after 1 week, and after 1 month. Data were analyzed with repeated measures of ANOVA (${\alpha}=.05$). RESULTS. The highest staining resistance was found in gl samples. There was no difference among gl, kc and cd (P>.05). Staining resistance of gl was significantly higher than that of bb (P<.05). Staining resistances of E and CS were significantly higher than those of L and ME (P<.05). CONCLUSION. Ceramic and composite polishing kits can be used for resin ceramics as a counterpart of glazing procedure used for full ceramic materials. Liquid polish has limited indications for resin ceramics.

A Study on the Measurement Accuracy and Linearity of the Mandibular Kinesiograph (Mandibular Kinesiograph의 선형충실도 및 계측정확도에 관한 연구)

  • Kim, In-Kwon
    • The Journal of the Korean dental association
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    • v.22 no.7 s.182
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    • pp.607-620
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    • 1984
  • The validity of the Kinesiegraph (MHG-5R) output was studied using a non-ferromagnetic positioning device within working range of a 3 cm wide by 4 cm deep by 5 cm high three dimensional lattice. To determine how well observed values of the M K G outputs may predict case values of actual measurements, relationships between those values were tested by viewing scatter plots and correlation between observed values and actual values. In order to devise some form of served values of the M k G outputs (volts), statistical regression analysis was performed. The statistical analysis showed that observed values and actual values were strongly correlated. However, high correlation does not necessarily mean perfect predictability of given linear models. The formulated models were not able to predict all of acutal values this is partly 엳 새the discreteness of dependent variables (actual values) and also is because of independent nature of observed values. In conclusion, unless the Kinesiograph is suitably modified, the inherent nonlinearity characteristic of the system output limits its application to the measurement of a limited range of jaw movements.

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The Design of Elliptic Function Bandpass Filter using Ceramic Coaxial Resonators (유전체 동축 공진기를 이용한 타원 함수 대역 통과 여파기의 설계)

  • 김정제;윤상원
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.10 no.6
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    • pp.805-814
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    • 1999
  • In this paper, elliptic function bandpass filters using ceramic coaxial resonators are designed. Since elliptic function filters have better performance of frequency selectivity than those based on Butterworth or Chebyshev, therefore it is possible to make better use of limited frequency resources. Elliptic function bandpass filters using ceramic coaxial resonators are designed for reducing it's size, weight, cost and for easy manufacturing and tuning. From measurements, an accurate resonator model is obtained and the coupling coefficient values are extracted. Based on these results, elliptic function bandpass filters are designed. The experimental results have shown that the 8th order elliptic function filter of 959 MHz center frequency with 28 MHz bandwidth using coaxial ceramic resonators have about more tan 17 dB return loss, 5 dB insertion loss, more than 20 dB attenuation at $f_c\pm$5 MHz.

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Lean Burn Characteristics in a Heavy Duty Liquid Phase LPG Injection SI Engine (대형 액상분사식 LPG 엔진의 희박연소특성에 관한 연구)

  • O, Seung-Muk;Kim, Chang-Eop;Lee, Jin-Uk;Kim, Chang-Gi;Gang, Geon-Yong;Bae, Chung-Sik
    • 연구논문집
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    • s.33
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    • pp.5-16
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    • 2003
  • Fuel distribution, combustion, and flame propagation characteristics of heavy duty engine with the liquid phase LPG injection(LPLI) were studied in a single cylinder engine. Optically accessible single cylinder engine and laser diagnostics system were built for quantifying fuel concentration by acetone PLIF(planar laser induced fluorescence) measurements. In case of Otto cycle engine with large bore size, the engine knock and thermal stress of exhaust manifold are so critical that lean burn operation is needed to reduce the problems. It is generally known that fuel stratification is one of the key technologies to extend the lean misfire limit. The formation of rich mixture in the spark plug vicinity was achieved by open valve injection. With higher swirl strength(Rs=3.4) and open valve injection, the cloud of fuel followed the flow direction and the radial air/fuel mixing was limited by strong swirl flow. It was expected that axial stratification was maintained with open-valve injection if the radial component of the swirling motion was stronger than the axial components. The axial fuel stratification and concentration were sensitive to fuel injection timing in case of Rs=3.4 while those were relatively independent of the injection timing in case of Rs2.3. Thus, strong swirl flow could promote desirable axial fuel stratification and, in result, may make flame propagation stable in the early stage of combustion.

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The Efficacy of Respiratory Exercise Programs in the Elderly Persons with Hemiplegia (고령 편마비 환자에 대한 호흡운동 적용의 효과)

  • Kim, Soo-Min
    • PNF and Movement
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    • v.5 no.2
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    • pp.63-71
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    • 2007
  • Objective : Respiratory muscle weakness and decreased chest mobility has been suggested to result from the deconditioning that accompanied activity level in chronic elderly stokes. The benefits of respiratory exercise programmes on exercise capacity and muscle strength in hemiplegia. This study aimed to determine the effects of selective inspiratory and expiratory muscles training and chest mobility exercise on patients with strokes to establish if an improved exercise capacity can be obtained in patients that are not limited in their daily activities. Methods & Intervention : Twelve patients were assigned to the intensive respiratory exercise group participated in a measures design that evaluated the subjects with pre-treatment and post-treatment. Thirteen subjects who were assigned to a control group received training with breathing exercise and resistance exercise of skeletal muscles. The subjects performed spirometry then undertook a 6-week programme of respiratory muscle and chest mobility training. Training for the two groups was carried out 2 times a week for 6 weeks. Measurements and Results : Spirometry(Forced Vital Capacity: FVC and Closed Circuit Spiromety: CCS) and thoracic mobility were measured before and after the 6 weeks. The experimental group improved significantly compared to control group in FVC, $FEV_1$, MVV, IRV and ERV, and upper chest wall expansion(p<0.05). No significant improvement was seen in thoracic mobility or lung function in control group(p>0.05). Conclusion : The major findings in this study were that a intensive 6week exercise programme of resistive breathing and chest mobility in patients with hemiplegia led to an increase in lung capacity. The resistive breathing exercise programme used here resulted in a significant increase in the chest excursion during breathing.

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Estimation of CO2 Emission from a Eutrophic Reservoir in Temperate Region (온대지역 부영양 저수지의 이산화탄소 배출량 산정)

  • Chung, Se-Woong;Yoo, Ji-Su;Park, Hyung-Seok;Schladow, S. Geoffrey
    • Journal of Korean Society on Water Environment
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    • v.32 no.5
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    • pp.433-441
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    • 2016
  • Many large dams have been constructed for water supply, irrigation, flood control and hydropower in Korea for the last century. Meanwhile, recent studies indicated that the artificial reservoirs impounded by these dams are major sources of carbon dioxide (CO2) to the atmosphere and relevant to global budget of green house gases. However, limited information is available on the seasonal variations of CO2 evasion from the reservoirs located in the temperate monsoon regions including Korea. The objectives of this study were to estimate daily Net Atmospheric Flux (NAF) of CO2 in Daecheong Reservoir located in Geum River basin of Korea, and analyze the influencing parameters that characterize the variation of NAF. Daily pH and alkalinity (Alk) data collected in wet year (2012) and dry year (2013) were used for estimating the NAFs in the reservoir. The dissolved inorganic carbon (DIC) was computed using the pH and Alk measurements supposing an equilibrium state among the carbonate species. The results showed seasonal variations of NAF; negative NAFs from May to October when the primary production of the reservoir increased with water temperature increase, while positive NAF for the rest of the period. Overall the reservoir acted as sources of CO2 to the atmosphere. The estimated NAFs were 2,590 and 771 mg CO2 m-2d-1 in 2012 and 2013, respectively, indicating that the NAFs vary a large extent for different hydrological years. Statistical analysis indicated that the NAFs are negatively correlated to pH, water temperature, and Chl-a concentration of the reservoir.

The Study on In-situ Measurement of Hydrogen Permeability through Polymer Electrolyte Membranes for Fuel Cells (연료전지용 고분자전해질막의 실시간 수소 투과도 측정법 연구)

  • Lim, Yoon Jae;Lee, Chang Hyun
    • Membrane Journal
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    • v.26 no.2
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    • pp.141-145
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    • 2016
  • Polymer electrolyte membranes (PEMs) are key components to determine electrochemical fuel cell performances, in addition to electrode materials. The PEMs need to satisfy selective transport behaviors to small molecules including gases and protons; the PEMs have to transport protons as fast as possible, while they should act as hydrogen barriers, since the permeated gas induces the thermal degradation of cathode catalyst, resulting in rapid electrochemical reduction. To date, limited tools have been used to measure how fast hydrogen gas permeates through PEMs (e.g., Constant volume/variable Pressure (time-lag) method). However, most of the measurements are conducted under vacuum where PEMs are fully dried. Otherwise, the obtained hydrogen permeance is easily changeable, which causes the measurement errors to be large. In this study, hydrogen permeation properties through Nafion212 used as a standard PEM are evaluated using an in-situ measurement system in which both temperature and humidity are controlled at the same time.

A Study on Switching Power Measurement of an Electrical Point Machine Using a Sensor (센서를 활용한 전기선로전환기의 전환력 측정에 대한 연구)

  • Lee, Jong-Hyun;Kim, Yong-Kyu
    • Journal of the Korean Society for Railway
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    • v.18 no.4
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    • pp.335-343
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    • 2015
  • The role of the turnouts installed on the points of railway branches and intersections is to safely switch the directions of trains. In case of abnormal forces applied to the turnouts due to extreme operating conditions, it is difficult in the current system to conduct accurate diagnosis and measuring. Moreover, the existing measurement devices for turnout switching power are mostly based on foreign technologies, which provide only limited information on the switching power; this information is insufficient for effective monitoring and maintenance. In this paper, through an analysis of the characteristics of the switching and locking function, the problems in turnout power measurements of current electrical point machines in Korea are examined. And based on the results, a new sensor-based method for measuring switching power is proposed.

A STUDY ON THE DIMENSIONAL CHANGES OF HEAT CURING ACRYLIC RESINS USING HOLOGRAPHIC INTERFEROMETRY (Holographic interferometry를 이용한 열중합 애크릴릭 레진의 변형에 관한 연구)

  • Park, Dong-Kwan;Chang, Ik-Tae;Kim, Kwang-Nam
    • The Journal of Korean Academy of Prosthodontics
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    • v.33 no.1
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    • pp.48-74
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    • 1995
  • Since heat curing acrylic resins undergo unavoidable dimensional changes following polymerization, adaptation can be altered. Until recently, although numerous studies on the dimensional changes of denture base were based on a microscopic technic that measures the relative displacement of a limited reference points on the denture base, but there have been few studies on the distortions of resins using holographic interferometry. Purpose of this study was to determine and compare the dimensional changes and fringe patterns of 4 heat curing acrylic resins, and observe the distortions of acrylic resin denture base by temperature change with the aid of the holographic interferometry. Holographic interferograms were taken on the resin specimens and acrylic resin denture base with the 10mW He-Ne laser and double exposure method. Comparison and analysis of fringe pattern on the recorded object surface was performed. The following results were obtained. 1. The dimensional changes for the high impact resin Lucitone 199 were statistically the greatest of all resins, and the rapid heat curing resin Premium super 20 were the least. 2. The most polymerization shrinkage of all materials occured in initial period of measurements, at this time the difference of polymerization shrinkage properties between resins was founded. 3. The stress distribution of specimens was seen by various type of fringe pattern which had directionality. 4. The polymerization shrinkage of resins was greatly influenced by temperature change. 5. The partial deformations of resin denture base were observed in 70 C and 90 C water.

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