• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.97-97
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• 2017

Plant genomes include heterochromatic loci that consist of repetitive sequences and transposable elements. LTR retrotransposon is the major class of transposons in advanced plants in terms of proportion in plant genome. The elements contribute not only to genome size but also to genome stability and gene expression. A number of cases have been reported transposon insertions near genic regions affect crop traits such as fruit pigments, stress tolerance, and yields. Functional LTR retrotransposons produce extrachromosomal DNA from genomic RNA by reverse transcription that takes place within virus-like-particles (VLPs). DECREASED DNA METHYLATION 1 (DDM1) plays important roles in maintaining DNA methylation of heterochromatin affecting all sequence contexts, CG, CHG, and CHH. Previous studies showed that ddm1 mutant exhibits massive transcription of retrotransposons in Arabidopsis, but only few of them were able to create new insertions into the genome. RNA-dependent RNA POLYMERASE 6 (RDR6) is known to function in restricting accumulation of transposon RNA by processing the transcripts into 21-22 nt epigenetically activated small interfering RNA (easiRNA). We purified VLPs and sequence cDNA to identify functional LTR retrotransposons in Arabidopsis ddm1 and ddm1rdr6 plants. Over 20 LTR copia and gypsy families were detected in ddm1 and ddm1rdr6 sequencing libraries and most of them were not reported for mobility. In ddm1rdr6, short fragments of ATHILA gypsy elements were detected. It suggests easiRNAs might regulate reverse transcription steps. The highest enriched element among transposon loci was previously characterized EVADE element. It has been reported that active EVADE element is more efficiently silenced through female germline than male germline. By genetic analyses, we found ddm1 and rdr6 mutation affect maternal silencing of active EVADE elements. DDM1-GFP protein accumulated in megaspore mother cell but was not found in mature egg cell. The fusion protein was also found in early embryo and maternal DDM1-GFP allele was more dominantly expressed in the embryo. We observed localization of DDM1-GFP in Arabidopsis and DDM1-YFP in maize and found the proteins accumulated in dividing zone of root tips. Currently we are looking at cell cycle dependency of DDM1 expression using maize system. Among 10 AGO proteins in Arabidopsis, AGO9 is specifically expressed in egg cell and shoot meristematic cells. In addition, mutation of AGO9 and RDR6 caused failure in maternal silencing, implying 21-22 nt easiRNA pathway is important for retrotransposon silencing in female gametophyte or/and early embryo. On the other hand, canonical 24 nt sRNA-directed DNA methylation (RdDM) pathways did not contribute to maternal silencing as confirmed by this study. Heat-activated LTR retrotransposon, ONSEN, was not silenced by DDM1 but the silencing mechanisms require RdDM pathways in somatic cells. We will propose distinct mechanisms of LTR retrotransposons in germline and somatic stages.

• Archives of Reconstructive Microsurgery
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• v.20 no.1
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• pp.32-37
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• 2011

Purpose: Canalicular laceration is relatively common due to its exposed, vulnerable location in case of facial trauma. Conjunctivodacryocystorhinostomy has been the standard treatment for canalicular obstruction secondary to unrepaired canalicular laceration. In spite of a high rate of relieving tear-duct obstruction, this method is noted to be associated with low rate of patient satisfaction and a number of complications. The goal of this study is to report the good results of delayed lacrimal stent implantation with Mini Monoka$^{(R)}$ for patients whose canaliculi were not repaired by initial surgery. Methods: From May of 2005 to February of 2007, four patients who underwent delayed lacrimal stent implantation using Mini Monoka$^{(R)}$ in canalicular laceration were retrospectively reviewed. First, the previous scar incision was made over the lower lid and we identified the cut end of proximal lacerated canaliculus. The Mini Monoka$^{(R)}$ was passed through the punctum to the cut end of the proximal lacerated canaliculus. We identified the cut end of the distal lacerated canaliculus, whose cut end has rolled white edges after careful excision of the scarred lid tissue. We then passed Mini Monoka$^{(R)}$ through the lacerated inferior canaliculus. Using an operating microscope, we placed three interrupted sutures of 8-0 Ethilon to join the canalicular ends. Silicone stent was left in place for 6 months. The mean age of the patients was 48.5 years (range, 35 to 59 years). The time interval from initial trauma to delayed lacrimal stent implantation was between 3 and 31 days. Follow-up periods ranged from 12 and 20 months. Results: The results of reconstruction were classified into normal, fair and poor. Patent lacrimal drainage systems were achieved in three of the four cases. In one case, epiphora was encountered. No cases of stent displacement, conjunctivitis or granuloma formation were encountered. Conclusion: Even though canalicular reconstruction was delayed for a long time, attempting canalicular reconstruction would be acceptable before considering secondary operations like dacryocystorhinostomy and conjunctivodacryocystorhinostomy.

• Journal of the Korean Society of School Health
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• v.12 no.2
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• pp.339-356
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• 1999

The purpose of this study is to analyze the factors which influence the college student sex behavior. The results can be used as the basis to establish health polices and a health promotion program to reduce the risks of sexual behavior of college students. The study subjects were random selected from college students in Kyonggido. The 335 subjects were selected from 4 schools including three colleges and one university. The self-administered questionnaires were distributed to 350 students, 335 of which were analyzed (after excluding the 15 incomplete or inaccurate data). The study took place between December 7-19, 1998. Regarding the analysis method, the SPSS program was used. The relationship between social and demographic characteristics and relevant factors was identified through Chi-squared test. The major findings of the study were follows: 1. Of the 335 respondents, thirty seven percent of men and ten percent of women had already experienced sexual intercourse. Significantly more males had experienced sexual experience than women. The majority of students, living with both their parents had less sexually experience than that living without their parents. There were religious differences, that students who believed in buddhism had more sexual experience than christian or catholic students. Students who reported drinking alcohol frequently had more sexual experience than those who didn't and it was statistically significant (${\chi}^2=47.6$ df=4 p=0.000). 2. Sexual awareness was relatively low. High level group of sex knowledge were less sexual experience than middle or low level group (${\chi}^2=6.038$ df=2 p=0.049). Women were more sexually aware than men (${\chi}^2=20.814$ df=2 p=0.000). Groups who had sisters and brothers were more sexually aware than those who few sisters and brothers. 3. Sixty one percent of respondents said that chastity before marriage should be kept but twenty four percent of them felt that it was unnecessary. Opponents to chastity before marriage had more sex experience than those for. It was statistically significant (${\chi}^2=48.967$ df=2 p=0.000). Those who agreed with the questionnaire point 'Even if people are not married, if they love each other, they can have sexual intercourse.' had more sexual intercourse than those who didn't agree. Those who agreed with the questionnaire point 'Even though people doesn't like, they can still have sexual intercourse.' had more sexual intercourse than those who disagree. On this point, men were three times higher of the opinion than women. So we can see the difference in the perception between men and women. As a result of this study, sexual awareness influences sexual attitudes and sexual attitudes influence sexual awareness influences sexual activity includes poor knowledge and uninformed attitudes about sex. Therefore sex education is needed and it is very important to reestablish concepts about sex.

• Journal of Radiation Protection and Research
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• v.24 no.1
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• pp.17-22
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• 1999

This study was carried out to evaluate the biochemical and morphological effects of ionizing radiation on mouse ovarian follicles. Immature mice (ICR, 3 week-old) were irradiated with a dose of $LD_{80(30)}$ at KAERI. The ovaries were collected after 6 hours, 12 hours, 1 day, and 2 days post irradiation. With the morphological basis of the histological staining with hematoxylin-eosin, immunohistochemical preparation using in situ 3'-end labeling was evaluated. Flowcytometric evaluation of DNA extracted from the whole ovary was performed. The percentage of $A_0$ (subpopulation of cells with degraded DNA and with lower DNA fluorescence than $G_0/G_1$ cells), apoptotic, cells in the cell cycle was significantly higher in the irradiated group than in the control group. The number of in situ 3'-end labeled follicles increased at 6 hours post irradiation. All the analyses represented that the ionizing radiation-induced follicular atresia was taken place via an apoptotic degeneration. Such a degeneration underwent very fast and acutely. Therefore, it is concluded that the radiation-induced follicular degeneration is, like the spontaneous atresia, mediated by an acute apoptosis of follicular granulosa cells. Flowcytometric evaluation of cell cycles can make the role for quantifying the atretic follicles and understanding the mechanism of the radiation-induced cell death.

• 대한공업교육학회지
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• v.34 no.1
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• pp.1-18
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• 2009

This study is aimed at analyzing vocational high school teachers' recognition of school consulting and educational demand for securing professionalism from school consultant training, to offer basic information for training school consultant. The survey was intended for vocational high school teachers who participated in the vocational high school teacher training course. The result of this study is as follows. First, vocational high school teachers have sympathy with the necessity of school consulting for 'change and survival' of vocational high school in the future, and recognize that successful school consulting will be possible when, in the first place, teachers and principal/deputy principal who are able to show leadership change for their recognition. Second, as the result of analyzing educational demand for school consultant training, demand for consulting business and case and experience area, like 'School consulting method and procedure', 'Sympathy creation technique', 'Case of innovation through consulting', was higher than for consulting theory. Third, as the result of surveying difference of importance recognition of school consultant training contents, according to experience of joining school consulting, 'Environmental change and necessity of consulting', 'Consulting status of domestic corporation and vocational high school', 'Consulting method and procedure', 'School status analysis technique' showed significant difference, and teachers who have experience showed higher average of importance than those who don't have experience. In conclusion, rather than consulting theory, education for consulting business and experience area and various cases-centered school consultant training program according to experience of joining school consulting should be developed.

• Information Systems Review
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• v.17 no.1
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• pp.141-169
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• 2015

Electronic financial accidents are constantly happening and these accidents are taking place by a combination of several causes such as technique, human, and structure. Among electronic financial accidents, personal information disclosure is most frequently happening and becomes big problems, because secondary damage like voice phishing causes great loss to society. This research model is that financial information security compliance affects the crisis response of financial institutions and financial authorities and these crisis responses affect financial information security trust. Research target is people who experienced the disclosure of their own financial information. For empirical verification, survey questionnaires were distributed and total 103 questionnaires were collected and analyzed. As results of data analysis, all hypotheses were accepted. First, financial information security compliance influenced the crisis response of financial institutions and authorities. Second, the crisis response of financial institutions and authorities affect financial information security trust. Third, at the moderating effect analysis, the importance of personal financial information moderated the effect of the crisis response of financial institutions on financial information security trust. And the disclosure level of personal financial information moderated the effect of the crisis response of financial authorities on financial information security trust.

• Journal of Korean Academy of Nursing
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• v.30 no.3
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• pp.799-812
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• 2000

This study adopts the phenomenological approach in order to explore the experience of urinary felt by the small island women and to find the meaning and structure of their experience, for the further understanding of them. This study succeeded in detecting five topics and three basic structure from eight participants, and followings are the comprehensive statement of them. The five topics include neglect of care after childbirth, unavoidable life in the tidal flat, shame which cannot be expressed even to their husbands, endless anxiety toward the expected future, and sad(dilemmatic) lived experience. The basic structure is that small island women who have urinary incontinence are apt to regard their disease as a natural destiny of women who fail to get adequate care after childbirth, and something to be endured to live in the seashore. They think of urinary incontinence as something so shameful that they cannot reveal it even to their husband and family. They believe that it even changes their personality since they must always stay alert in order to cope with the situation; for example, when it takes place unexpectedly, like too often to go to toilet, to change the underwears, to wake up in the middle of the night to go to toilet, to try not to laugh loudly, or to have showers. In addition, they accept it as a natural process of aging and incurable disease, and they consider themselves already ruined on the way of becoming uglier. They show dilemmatic abandonment: give it up unwillingly but at the same time think it is natural for others too. The unique experience of small island women with urinary incontinence implied in those statement are inseparable with the specific conditions for survival in the island. Unlike other diseases, it is considered the result of traditionally poor care after childbirth. However this misunderstanding that it is a natural phenomena for all the women who experience childbirth and aging and thereby incurable leads to an undesirable attitude toward urinary incontinence. According to the analysis, environmental conditions specific for small islands make the women there have distinct and unique experience concerned with urinary incontinence. Consequently, the future nursing plan for urinary incontinence in the small island area must be made and enforced with the consideration of these specific phenomenological meanings. Modern Korean nursing has basically been centered to hospital or urban areas. Besides, nursing intervention has long depended upon the research of western countries. This research, however, shows how greatly the regional and cultural characteristics influence the understanding of a certain disease, and is expected to make more specific and in-depth nursing approach enable for those who have urinary incontinence in small islands.

• The KIPS Transactions:PartD
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• v.12D no.1 s.97
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• pp.165-178
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• 2005

A product classification scheme is the foundation on which product databases are designed, and plays a central role in almost all aspects of management and use of product information. It needs to meet diverse user views to support efficient and convenient use of product information. It needs to be changed and evolved very often without breaking consistency in the cases of introduction of new products, extinction of existing products, class reorganization, and class specialization. It also needs to be merged and mapped with other classification schemes without information loss when B2B transactions occur. For these requirements, a classification scheme should be so dynamic that it takes in them within right time and cost. The existing classification schemes widely used today such as UNSPSC and eCl@ss, however, have a lot of limitations to meet these requirements for dynamic features of classification. Product information implies a plenty of semantics such as class attributes like material, time, place, etc., and integrity constraints. In this Paper, we analyze the dynamic features of product databases and the limitation of existing code based classification schemes, and describe the semantic classification model proposed in [1], which satisfies the requirements for dynamic features of product databases. It provides a means to explicitly and formally express more semantics for product classes and organizes class relationships into a graph.

• Journal of Computing Science and Engineering
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• v.6 no.1
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• pp.40-50
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• 2012

Increase in the number of older people due to demographic changes poses great challenges to the social healthcare systems both in the Western and as well as in the Eastern countries. Support for older people by formal care givers leads to enormous temporal and personal efforts. Therefore, one of the most important goals is to increase the efficiency and effectiveness of today's care. This can be achieved by the use of assistive technologies. These technologies are able to increase the safety of patients or to reduce the time needed for tasks that do not relate to direct interaction between the care giver and the patient. Motivated by this goal, this contribution focuses on applications of acoustic technologies to support users and care givers in ambient assisted living (AAL) scenarios. Acoustic sensors are small, unobtrusive and can be added to already existing care or living environments easily. The information gathered by the acoustic sensors can be analyzed to calculate the position of the user by localization and the context by detection and classification of acoustic events in the captured acoustic signal. By doing this, possibly dangerous situations like falls, screams or an increased amount of coughs can be detected and appropriate actions can be initialized by an intelligent autonomous system for the acoustic monitoring of older persons. The proposed system is able to reduce the false alarm rate compared to other existing and commercially available approaches that basically rely only on the acoustic level. This is due to the fact that it explicitly distinguishes between the various acoustic events and provides information on the type of emergency that has taken place. Furthermore, the position of the acoustic event can be determined as contextual information by the system that uses only the acoustic signal. By this, the position of the user is known even if she or he does not wear a localization device such as a radio-frequency identification (RFID) tag.

• Journal of Korean Ophthalmic Optics Society
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• v.7 no.1
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• pp.15-20
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• 2002

The Hydroxyethyl methacrylate(HEMA) was developped in 1960's and later the use of contact lenses increased. At present more and more people use RGP contact lenses. Due to the wide range of optical corrections available and due to their high oxygen transmission Japanese and Korean people like them. Nevertheless one of nine CL users(beginners) stopped wearing the CL within the first year. We conducted a survey to evaluate the level of patient compliance in lens care and maintenance and to assess practitioner knowledge of contact lens information. Generally 6% of contact lens wearers drop out per year. The aim of this research was to find out what opticians can do to reduce the drop-out rate by getting information on handling, on general data of the patient and on optical prescription. Lens wearer were asked to complete a 10 question survey that focused on lens care-wear modality, lens replacement, storage, symptoms and case disinfection. There were total 180 participants in the study. Most of contact lens wearers have an inadequate understanding of contact lens care system. Therefore, it is important that contact lens specialists place more emphasis on practitioner education about general contact lens fields and reinforce patient education during the dispensing visit.