• 펄프종이기술
• /
• 제42권5호
• /
• pp.15-23
• /
• 2010

Bio-ethanol is the most potential next generation automotive fuel for reducing both consumption of crude oil and environmental pollution from renewable resources such as wood, forest residuals, agricultural leftovers and urban wastes. Lignocellulosic based materials can be broken down into individual sugars. Therefore, saccharification is one of the important steps for producing sugars, such as 6-C glucose, galactose, mannose and 5-C xylose, mannose and rhamnose. These sugars can be further broken down and fermented into ethanol. The main objective of this research is to study the feasibility and optimize saccharification and fermentation process for the conversion of lignocellulosic biomass to low cost bioethanol.

• 한국연소학회:학술대회논문집
• /
• 한국연소학회 2015년도 제51회 KOSCO SYMPOSIUM 초록집
• /
• pp.331-332
• /
• 2015

• 한국건축시공학회:학술대회논문집
• /
• 한국건축시공학회 2023년도 가을학술발표대회논문집
• /
• pp.77-78
• /
• 2023

This paper experimentally examined the recycling of combustion by-products emitted from a combined heat and power plant using lignocellulosic biomass fuel. Physical and chemical analyzes were performed on Bio-SRF and three types of wood pellet combustion by-product samples (fly-ash, FA). As a result of the experiment, the compressive strength of mortar substituted with 5, 10, and 20% of FA compared to the cement weight was found to be excellent, and its recyclability was confirmed as a substitute for existing admixtures.

• Journal of Microbiology and Biotechnology
• /
• 제9권3호
• /
• pp.297-302
• /
• 1999

Simultaneous saccharification and fermentation (SSF) experiments were carried out with a lignocellulosic biomass. The effects of temperature on enzymatic saccharification and the ethanol fermentation were also investigated. The batch SSF process gave a final ethanol concentration of 10.44 g/l and equivalent glucose yield of 0.55 g/g, which was increased by 67％ or higher over the saccharification at 42℃. The optimal operating condition was found to vary in several parameters, such as the transmembrane pressure, permeation rate, and separation coefficient, related to the SSF combined with membrane system (semi-batch system). When the fermentation was operated in a semi-batch mode, the efficiency of the enzymes and yeast lasted three times longer than in a batch mode.

• Mycobiology
• /
• 제34권4호
• /
• pp.159-165
• /
• 2006

One of the most economically viable processes for the bioconversion of many lignocellulosic waste is represented by white rot fungi. Phanerochaete chrysosporium is one of the important commercially cultivated fungi which exhibit varying abilities to utilize different lignocellulosic as growth substrate. Examination of the lignocellulolytic enzyme profiles of the two organisms Phanerochaete chrysosporium and Rhizopus stolonifer show this diversity to be reflected in qualitative variation in the major enzymatic determinants (ie cellulase, xylanase, ligninase and etc) required for substrate bioconversion. For example P. chrysosporium which is cultivated on highly lignified substrates such as wood (or) sawdust, produces two extracellular enzymes which have associated with lignin deploymerization. (Mn peroxidase and lignin peroxidase). Conversely Rhizopus stolonifer which prefers high cellulose and low lignin containg substrates produce a family of cellulolytic enzymes including at least cellobiohydrolases and ${\beta}-glucosidases$, but very low level of recognized lignin degrading enzymes.

• 한국건축시공학회지
• /
• 제23권6호
• /
• pp.663-671
• /
• 2023

본 연구에서는 목질계 바이오매스(Bio-SRF, 우드펠릿)를 연료로 사용하는 발전소 3곳에서 배출된 4종의 목질계 플라이애시(FA)의 재활용성을 실험적으로 검토하였다. FA의 물리 분석(입형, 입도분포, 분말도, 밀도)과 화학 분석(화학조성, 중금속 함량)을 수행하였고, KS L 5405에 준하여 혼입률 5~20%의 모르타르 시험체를 제작하고 플로우와 압축강도를 측정하였다. 4종의 FA는 입경 약 20~30㎛, 밀도 약 2.3~2.5g/cm³, 분말도 2,600~4,900cm²/g, SiO₂, Al₂O₃, Fe₂O₃, CaO 성분이 약 50~90%를 차지하고 있으며, 염소와 SiO₂ 함량을 배제하고 KS L 5405의 FA 2, 3형과 유사한 특성을 보였다. 모르타르 혼입 평가 결과, 펠릿 연소 FA는 5~20% 혼입 범위에서 모르타르 압축강도는 34~47MPa로 측정되었다. 목질계 연료 100%를 연소한 FA는 혼화재 대체재로서 재활용성이 높다고 평가된다.

• Mass Spectrometry Letters
• /
• 제4권1호
• /
• pp.1-9
• /
• 2013

Fungal biotechnology has been well established in food and healthcare sector, and now being explored for lignocellulosic biorefinery due to their great potential to produce a wide array of extracellular enzymes for nutrient recycling. Due to global warming, environmental pollution, green house gases emission and depleting fossil fuel, fungal enzymes for lignocellulosic biomass refinery become a major focus for utilizing renewal bioresources. Proteomic technologies tender better biological understanding and exposition of cellular mechanism of cell or microbes under particular physiological condition and are very useful in characterizing fungal secretome. Hence, in addition to traditional colorimetric enzyme assay, mass-spectrometry-based quantification methods for profiling lignocellulolytic enzymes have gained increasing popularity over the past five years. Majority of these methods include two dimensional gel electrophoresis coupled to mass spectrometry, differential stable isotope labeling and label free quantitation. Therefore, in this review, we reviewed more commonly used different proteomic techniques for profiling fungal secretome with a major focus on two dimensional gel electrophoresis, liquid chromatography-based quantitative mass spectrometry for global protein identification and quantification. We also discussed weaknesses and strengths of these methodologies for comprehensive identification and quantification of extracellular proteome.

• 한국미생물·생명공학회지
• /
• 제50권1호
• /
• pp.122-125
• /
• 2022

The cellulose-fed microbial fuel cell (MFC) is a biotechnological process that directly converts lignocellulosic materials to electricity without combustion. In this study, the cellulose-fed, MFC-integrated thermophilic bacterium, Bacillus sp. WK21, with endoglucanase and exoglucanase activities of 1.25 ± 0.08 U/ml and 0.95 ± 0.02 U/ml, respectively, was used to generate electricity at high temperatures. Maximal current densities of 485, 420, and 472 mA/m² were achieved when carboxymethyl cellulose, avicel cellulose, and cellulose powder, respectively, were used as substrates. Their respective maximal power was 94.09, 70.56, and 89.30 mW/m³. This study demonstrates the value of the novel use of a cellulase-producing thermophilic bacterium as a biocatalyst for electricity generation in a cellulose-fed MFC.

• 한국신재생에너지학회:학술대회논문집
• /
• 한국신재생에너지학회 2008년도 추계학술대회 논문집
• /
• pp.71-73
• /
• 2008

Different biosolid fuel (wood pellet) properties between trunk and root of pine (Pinus densiflora) biomass were investigated. Trunk has more organic solvent extracts and Klason lignin content which has higher heating values than root biomass component. In root biomass, polysaccharides content was higher than trunk biomass. Based on Higher Heating Value (HHD) analysis and ash content, trunk biomass showed better solid fuel characteristics than root biomass. But pine root biomass had lower HHD than trunk biomass, its HHD values were higher than other hardwood or annual plant lignocellulosic biomass.

• 한국분무공학회지
• /
• 제21권3호
• /
• pp.144-154
• /
• 2016

This review will be concentrated on the spray characteristics of biobutanol and its blends fuels in internal combustion engines including compression ignition, spark ignition and gas turbine engines. Butanol can be produced by fermentation from sucrose-containing feedstocks, starchy materials and lignocellulosic biomass. Among four isomers of butanol, n-butanol and iso-butanol has been used in CI and SI engines. This is due to higher octane rating and lower water solubility of both butanol compared with other isomers. The researches on the spray characteristics of neat butanol can be classified into the application to CI and SI engines, particularly GDI engine. Two empirical correlations for the prediction of spray angle for butanol as a function of Reynolds number was newly suggested. However, the applicability for the suggested empirical correlation is not yet proved. The butanol blended fuels used for the investigation of spray characteristics includes butanol-biodiesel blend, butanol-gasoline blend, butano-jet A blend and butanol-other fuel blends. Three blends such as butanol/ethanol, butanol/heptane and butanol/heavy fuel oil blends are included in butanol-other fuel blends. Even though combustion and emission characteristics of butanol/diesel fuel blend in CI engines were broadly investigated, study on spray characteristics of butanol/diesel fuel blend could not be found in the literature. In addition, the more study on the spray characteristics of butanol /gasoline blend is required.