• International Journal of Automotive Technology
• /
• v.7 no.3
• /
• pp.353-360
• /
• 2006

The nominal structural stress calculation method proposed by Radaj has included some problems as follows: (a) How the value of the diameter D is decided in the method; (b) It is not possible to estimate nominal structural stress of the spot welded joints with the balanced sheet in-plane load that no general loads are obtained by FE shell analysis. In this paper, the new method for calculating nominal structural stress was proposed to solve above-mentioned problems. The proposed method calculates the nominal structural stress through the circular plate theory in theory of elasticity. This theoretical analysis uses not only general loads but also nodal displacements around spot welding provided by FE shell analysis as boundary condition. Fatigue test data of various spot-welded joints could be arranged in a narrow bandwidth on S-N chart using the nominal structural stresses calculated by proposed method. The fatigue life prediction method using the proposed method for calculating nominal structural stress is useful for the prior evaluation technique that can predict the fatigue life of spot welding by CAE.

• Journal of Plant Biotechnology
• /
• v.37 no.4
• /
• pp.370-378
• /
• 2010

This review describes the stratagies of development of virus-resistant Alstroemeria plants using the genetic modification system. Despite of increasing of its importance in cut flower market, improvements of some horticultuirally important traits such as fragrance, long vase-life, virus resistance and tolerance against abiotic stresses are lack of the breeding program in Alstroemeria. Of these traits, virus-resistance is quite difficult to develop in Alstroemeria plants due to the limitations of genetic variation in the existed germplasm. To extend the genetic variation, plant biotechnological techniques such as genetic transformation and tissue culture should be combined to develop virus-resistant line in Alstroemeria. In this review, several strategies for the generation of virus-resistance by using natural resistance genes, pathogen-derived genes and other sources including pathogen-derived proteins, virus-specific antibodies and ribosome-inactivating proteins are presented. Also, brief histories of breeding, tissue culture, and transformation system in Alstroemeria plants are described to inderstand of the application of transgenic approach for the development of virus-resistance in Alstroemeria species.

• Structural Engineering and Mechanics
• /
• v.35 no.3
• /
• pp.283-299
• /
• 2010

This paper addresses the numerical simulation of fatigue crack growth in arbitrary 2D geometries under constant amplitude loading by the using a new finite element software. The purpose of this software is on the determination of 2D crack paths and surfaces as well as on the evaluation of components Lifetimes as a part of the damage tolerant assessment. Throughout the simulation of fatigue crack propagation an automatic adaptive mesh is carried out in the vicinity of the crack front nodes and in the elements which represent the higher stresses distribution. The fatigue crack direction and the corresponding stress-intensity factors are estimated at each small crack increment by employing the displacement extrapolation technique under facilitation of singular crack tip elements. The propagation is modeled by successive linear extensions, which are determined by the stress intensity factors under linear elastic fracture mechanics (LEFM) assumption. The stress intensity factors range history must be recorded along the small crack increments. Upon completion of the stress intensity factors range history recording, fatigue crack propagation life of the examined specimen is predicted. A consistent transfer algorithm and a crack relaxation method are proposed and implemented for this purpose. Verification of the predicted fatigue life is validated with relevant experimental data and numerical results obtained by other researchers. The comparisons show that the program is capable of demonstrating the fatigue life prediction results as well as the fatigue crack path satisfactorily.

• The Plant Pathology Journal
• /
• v.38 no.4
• /
• pp.372-382
• /
• 2022

Soybean is an important source of protein and for a wide range of agricultural, food, and industrial applications. Soybean is being affected by Xanthomonas citri pv. glycines, a causal pathogen of bacterial pustule disease, result in a reduction in yield and quality. Diverse microbial communities of plants are involved in various plant stresses is known. Therefore, we designed to investigate the microbial community differentiation depending on the infection of X. citri pv. glycines. The microbial community's abundance, diversity, and similarity showed a difference between infected and non-infected soybean. Microbiota community analysis, excluding X. citri pv. glycines, revealed that Pseudomonas spp. would increase the population of the infected soybean. Results of DESeq analyses suggested that energy metabolism, secondary metabolite, and TCA cycle metabolism were actively diverse in the non-infected soybeans. Additionally, Streptomyces bacillaris S8, an endophyte microbiota member, was nominated as a key microbe in the healthy soybeans. Genome analysis of S. bacillaris S8 presented that salinomycin may be the critical antibacterial metabolite. Our findings on the composition of soybean microbiota communities and the key strain information will contribute to developing biological control strategies against X. citri pv. glycines.

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.80.1-80
• /
• 2003

Several plant and microbial genes that could confer disease resistance in transgenic rice plants are being cloned and characterized. We are currently constructing transgenic rice lines that overexpress the gene products, such as a galactinol synthase, a defensin, and a bacterial ACC deaminase. Subtractive hybridization of a rice cDNA library constructed from the Xanthomonas oryzae-infected ice leaves resulted in isolation of many inducible cDNA clones including a elongation factor EF2, a oryzain alpha, a catalase, a aldehyde dehydrogenase, a S-adenosylmethionine synthetase, a caffeic acid O-methyltransferase, a glyceraldehyde-3-phosphate dehydrogenase, a light-regulated protein, nKY transcription factors, and a nucleotide diphosphate kinase. Some genes among those may be useful genetic sources for construction of disease resistant transgenic rice. Full lengths of the rice OsFIERG and a rice oryzain genomic clones were cloned, and serial deletion fragments of the promoter regions of these genes were fused with GUS reporter gene in pCAMBIA1201, respectively. Promoter activities of these constructs will be examined upon various stresses and Pathogen infections to obtain the pathogen specific inducible-promoter. This work was supported by a grant from BioGreen 21 Program, Rural Development Administration, Republic of Korea.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.33 no.2
• /
• pp.102-107
• /
• 2016

Stress may be defined as any modification of environmental parameters that leads to a response by biological organisms. Stresses that affect biolpgical structures may be nonthermal, such as ultraviolet radiation, pH, or salinity, or thermal. Temperture is one of the major stresses that all living organism face. The major effects of cold(low emperature) are decrease of membrane fluidity and the stabilization of secondary structures of RNA and DNA in the cells, which may effect the efficiency of translation, transcription, and DNA replication. In this study, we focus on discovering the genes that are expressed by the cold(low temperature) stress in the silkworm. In cold(low temperature) stress test, we found 100% survive from cold stress at $0^{\circ}C$ up to 12h and $-5^{\circ}C$ up to 2h, and then, survive rate was rapidly decrease in silkworm. Thereafter two whole genes have selected by SSH(Suppression subtractive hybridization). (GenBank accession : GQ149511, GQ338156)

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.7 no.1
• /
• pp.43-54
• /
• 1987

Cumulative damage characteristics of concrete, on which rapetitive loads are applied, are investigated. Preliminarily, a series of uniaxial compression tests on cylindrical specimens of plain concrete is carried out to find out that, among various factors, stress levels of repetitive loadings and loading order are the most governing factors of cumulative damage of concrete. Based on this preliminary study, fatigue tests are carried out applying two levels of stresses, stepwisely. As a result, it is found that characteristics of cumulative damage of concrete are governed by nonlinear relationships and do not follow Miner's linear theory. It is also observed that cumulative damage characteristics and static strengths of concrete vary with loading history of stresses. Empirical equations which may be useful in predicting fatigue characteristics and remaining life of concrete stuctures are proposed for concrete subjected two stress levels.

• Journal of the Korean Society of Manufacturing Technology Engineers
• /
• v.9 no.3
• /
• pp.9-17
• /
• 2000

The use of ceramic inserts in steel forging tools offers significant technical and economic advantages over other materi-als of manufacture. These potential benefits can however only be realised by optimal design of the tools so that the ceramic insert are not subjected to stresses that led to their premature failure. In this paper the data on loading of the tools is determined from a commercial forging simulation package as the contact stress distribution on the die-workpiece interface and as temperature distributions in the die. This data can be processed as load input data for a finite-element die-stress analysis. Process simulation and stress analysis are thus combined during the design and a data exchange program has been developed that enables optimal design of the dies taking into account the elastic detections generated in shrink fitting the die inserts and that caused by the stresses generated in the forging process. The stress analysis of the dies is used to determine the stress conditions on the ceramic insert by considering contact and interference effects under both mechanical and thermal loads. Simulation results have been validated as a result of experimental investigation. Laboratory tests on ceramic insert dies have verified the superior performance of the Zirconia and Silicon Nitride ceramic insert in order to prolong maintenance life.

• Proceedings of the KSME Conference
• /
• 2000.11a
• /
• pp.316-321
• /
• 2000

The role of fatigue on the surface damage of 5% chrome cold milling work roll is evaluated. Uniaxial and torsional fatigue tests are carried out, and the baseline data for fatigue life evaluation are established. An appropriate multiaxial fatigue parameter is developed from the fatigue data. Fatigue tests are also performed under compressive mean stresses, and a mean stress model is formulated. A computer program is developed to assess the interaction of fatigue and grinding of the roll. The fatigue damage is computed for selected servicing conditions. It is found that the fatigue damage can be an important issue when the effect of mean stress is ignored, however the fatigue damage is negligibly small when the effect of mean stress including the residual stresses currently used is fully accounted. The result indicates that spalling due to the growth of thermal shock cracks is more important than fatigue damage in roll surface failure.

• Proceedings of the KWS Conference
• /
• 2005.11a
• /
• pp.79-81
• /
• 2005

Bridges constructed recently are preferred to have long spans and simple structure details considering not only the function as bridge but scenic beauty, maintenance, construction term and life cycle cost, etc. Therefore, they require high performance steels like extra-thick plate steels and TMCP steels. A TMCP steel produced by themo-mechanical control process is now spot lighted due to the weldability for less carbon equivalent. It improved at strength and toughness in microstructure. Recently, the SM570-TMC steel which is a high strength TMCP steel whose tensile strength is 600MPa has been developed and applied to steel structures. But, for the application of this steel to steel structures, it is necessary to elucidate not only the material characteristics but also the mechanical characteristic of welded joints. In this study, the characteristics of residual stresses in welded joints of SM570-TMC steel were studied through the three-dimensional thermal elastic-plastic analyses on the basis of mechanical properties at high temperatures obtained from the elevated temperature tensile test.