International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Identification of the genes which related cold (low temperature) stress in Bombyx mori

  • Kang, Min-Uk (Department of Analysis & Certification, FACT) ;
  • Choi, Kwang-Ho (Department of Agricultural Biology, NAAS, RDA) ;
  • Park, Kwan-Ho (Department of Agricultural Biology, NAAS, RDA) ;
  • Nho, Si-Kab (College of Agricultural and Life science, Kyungpook National University)
  • Received : 2016.10.20
  • Accepted : 2016.11.04
  • Published : 2016.12.31
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Abstract

Stress may be defined as any modification of environmental parameters that leads to a response by biological organisms. Stresses that affect biolpgical structures may be nonthermal, such as ultraviolet radiation, pH, or salinity, or thermal. Temperture is one of the major stresses that all living organism face. The major effects of cold(low emperature) are decrease of membrane fluidity and the stabilization of secondary structures of RNA and DNA in the cells, which may effect the efficiency of translation, transcription, and DNA replication. In this study, we focus on discovering the genes that are expressed by the cold(low temperature) stress in the silkworm. In cold(low temperature) stress test, we found 100% survive from cold stress at $0^{\circ}C$ up to 12h and $-5^{\circ}C$ up to 2h, and then, survive rate was rapidly decrease in silkworm. Thereafter two whole genes have selected by SSH(Suppression subtractive hybridization). (GenBank accession : GQ149511, GQ338156)

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References

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