• Title/Summary/Keyword: life stresses

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Study on CsRCI2D and CsRCI2H for improvement of abiotic stress tolerance in Camelina sativa L.

  • Lim, Hyun-Gyu;Kim, Hyun-Sung;Kim, Jung-Eun;Ahn, Sung-Ju
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.196-196
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    • 2017
  • Oilseed crop Camelina (Camelina sativa L.) is a suitable for biodiesel production that has high adaptability under low-nutrient condition like marginal land and requires low-input cost for cultivation. Enhanced abiotic stress tolerance of Camelina is very important for oil production under the wide range of different climate. CsRCI2s (Rare Cold Inducible 2) are related proteins in various abiotic stresses that predicted to localized at plasma membrane (PM) and endoplasmic reticulum (ER). These proteins are consist of eight-family that can be divided into tail (CsRCI2D/E/F/G) and no-tail (CsRCI2A/B/E/H) type of C-terminal. However, it is still less understood the function of C-terminal tail. In this study, CsRCI2D/H genes were cloned through gateway cloning system that used pCB302-3 as destination vector. And we used agrobacterium-mediated transformation system for generation of overexpression (OX) transformants. Overexpression of target gene was confirmed using RT-PCR and segregation ratio on selection media. We analyzed physiological response in media and soil under abiotic stresses using CsRCI2D and CsRCI2H overexpression plant. To compare abiotic stresses tolerance, wild type and CsRCI2D/H OX line seeds were sown on agar plate treated with various NaCl and mannitol concentration for 7 days. In the test of growth rate under abiotic stress on media, CsRCI2H OX line showed similar to NaCl and mannitol stress. In the other hand, CsRCI2D OX line showed to be improved stress tolerance that especially increased in 200mM NaCl but was similar on mannitol media. In greenhouse, WT and CsRCI2D/H OX lines for physiological analysis and productivity under abiotic stresses were treated 100, 150, 200mM NaCl. Then it was measured various parameters such as leaf width and length, plant height, total seed weight, flower number, seed number. CsRCI2H OX line in greenhouse did not show any changes in physiological parameters but CsRCI2D OX line was improved both physiological response and productivity under NaCl stress. Among physiological parameters of CsRCI2D OX line under NaCl stress, leaf length and width were observed shorter than WT but it were slightly longer than WT in 200mM NaCl stress. Furthermore, total seed weight of CsRCI2D OX line under stress displayed to decrease than WT in normal condition, but it was gradually raised with increasing NaCl stress then more than WT relatively. These results suggested CsRCI2D might be contribute to improve abiotic stress tolerance. However, function of CsRCI2H is need to more detail study. In conclusion, overexpression of CsRCI2s family can generate various environmental stress tolerance plant and may improve crop productivity for bio-energy production.

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The Psychosocial Adjustment of High-Risk Adolescents in Poverty (고위험 빈곤청소년의 심리사회적 적응)

  • Park, Hyun-Sun
    • Korean Journal of Social Welfare
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    • v.37
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    • pp.195-216
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    • 1999
  • Adolescents whose families receive public assistance are primary interest in the article because they experience disproportionate shares of the burden of poverty and psychosocial stress and are at substantially high risk. To explain the psychosocial stresses of high-risk adolescents in poverty, this study analyzed the inter-relations and interactions of major variables: 1) stress related life condition, 2)individual & environmental resources, 3) emotional, behavioral, and academic adjustment. Data came from the survey of 351 students living in the families receiving public assistance. The major findings were as follows. High-risk adolescents in poverty suffered from chronic stresses related life conditions, including family environmental stress, financial & medical stress and stigmatic stress. As the effects of stress increased, depression/anxiety and delinquency of poor youth increased. The psychosocial adjustment of poor youth at risk was most effected by the stress resulted from family environment. Though they were suffered from stresses related poor environment, adolescents in poverty adjusted well, if they had individual and environmental resources including self-esteem democratic parenting and supportive school climate. In conclusion, this study confirmed that emotional, behavioral, and academic adjustment of adolescents in poverty was affected by various stresses from life conditions and individual and environmental resources. In order to improve adjustment of poor youth at risk, life conditions have to be improved and psychosocial resources to be increased. These findings have provided practical implications for social workers helping high-risk adolescents in poverty.

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Applicability of Hammer-Peening Treatment for Fatigue Life Improvement of Fatigue Damaged Weld Joints (피로손상된 용접이음의 피로수명 향상을 위한 햄머피닝 처리법의 적용)

  • Kim, In Tae;Park, Min Ho;Cheung, Jin Hwan
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.17 no.3
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    • pp.48-55
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    • 2013
  • In this study, fatigue tests were performed on longitudinal out-of-plane gusset fillet welded joints and transverse non-load-carrying cruciform rib fillet welded joints, and then applicability of hammer-peening treatment on improvement of fatigue life for fatigue damaged weld joints were investigated. Fatigue tests were carried out on three types of gusset and rib welded specimens: as-welded specimens, post-weld hammer peened specimens and hammer peened specimens at 50% of as-welded specimen's fatigue life. Before and after hammer peening treatment, the geometry of weld toes and surface stresses near weld toes were measured. As a result of hammer peening treatment, compressive residual stresses of 30-83MPa were introduced near weld toes of the gusset and rib welded joints, and 130% increase in fatigue life and fatigue limit of the welded joints could be realized by hammer peening treatment at 50% fatigue life of as-welded conditions.

Improvement of heat and drought photosynthetic tolerance in wheat by overaccumulation of glycinebetaine

  • Wang, Gui-Ping;Hui, Zhen;Li, Feng;Zhao, Mei-Rong;Zhang, Jin;Wang, Wei
    • Plant Biotechnology Reports
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    • v.4 no.3
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    • pp.213-222
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    • 2010
  • Within their natural habitat, crops are often subjected to drought and heat stress, which suppress crop growth and decrease crop production. Causing overaccumulation of glycinebetaine (GB) has been used to enhance the crop yield under stress. Here, we investigated the response of wheat (Triticum aestivum L.) photosynthesis to drought, heat stress and their combination with a transgenic wheat line (T6) overaccumulating GB and its wild-type (WT) Shi4185. Drought stress (DS) was imposed by controlling irrigation until the relative water content (RWC) of the flag leaves decreased to between 78 and 82%. Heat stress (HS) was applied by exposing wheat plants to $40^{\circ}C$ for 4 h. A combination of drought and heat stress was applied by subjecting the drought-stressed plants to a heat stress as above. The results indicated that all stresses decreased photosynthesis, but the combination of drought and heat stress exacerbated the negative effects on photosynthesis more than exposure to drought or heat stress alone. Drought stress decreased the transpiration rate (Tr), stomatal conductance (Gs) and intercellular $CO_2$ concentration (Ci), while heat stress increased all of these; the deprivation of water was greater under drought stress than heat stress, but heat stress decreased the antioxidant enzyme activity to a greater extent. Overaccumulated GB could alleviate the decrease of photosynthesis caused by all stresses tested. These suggest that GB induces an increase of osmotic adjustments for drought tolerance, while its improvement of the antioxidative defense system including antioxidative enzymes and antioxidants may be more important for heat tolerance.

Research on Families in Later Life since 1980: products and Prospects (1980년 이후의 노년기가족 연구: 성과와 과제)

  • 신화용
    • Journal of Families and Better Life
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    • v.14 no.2
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    • pp.35-50
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    • 1996
  • This paper is a review and assessment of 1122 articles on families in later life from 5 journals published during 1980 and 1995. theoretical and methodological issues family relationships in later life social support network caregiving attitudes and stress life satisfaction and stress of the aged and welfare system for the aged are the major areas examined. The review indicates that this area of research in quantity has increased rapidly since 195. Relationships between aged parents and their adult children focusing on caregiving attitudes behavior and stresses for supporting their parents among the children and adjustment/life satisfaction of the aged are dominantly investigated. However most of the research are non-theoretical and descriptive in nature and the influences of socio-economic variables such as sex health economic status and educational level on dependent variables are widely investigated. Future research questions and issues under the sub-ares of families in lat r life is provided. Further directions and suggestions for future research works on families in later life in general are provided with particular emphasis on conceptual and methodological issues.

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Analysis of Allowable Stresses of Machine Graded Lumber in Korea (국내 기계등급구조재의 허용응력 분석)

  • Hong, Jung-Pyo;Oh, Jung-Kwon;Park, Joo-Saeng;Han, Yeon Jung;Pang, Sung-Jun;Kim, Chul-Ki;Lee, Jun-Jae
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.4
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    • pp.456-462
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    • 2015
  • 365 pieces of domestic $38{\times}140{\times}3600mm$ Red pine structural lumber were machine graded conforming to a softwood structural lumber standard (KS F 3020). The allowable bending stresses calculated for each grade were compared with the values currently tabulated in the standard. Four calculation methods for lower $5^{th}$ percentile bending stress were non-parametric estimation with 75% confidence level, 2-parameter and 3-parameter Weibull distribution fit, and bending modulus of rupture (MOR)-modulus of elasticity (MOE) regression based method. Only the data set of Grades E8, E9, and E10 were statistically eligible for the $5^{th}$ percentile calculation. The MOR-MOE regression based method only was able to estimate the lower $5^{th}$ percentile values theoretically for the full range of grades. The results showed that all allowable bending stresses calculated were lower than the design values tabulated in the standard. This implies that the current machine grading system has the pitfall of structural safety. Improvement in current machine grading system could be achieved by introducing the bending strength and stiffness combination grade system.

A Study on Reliability Estimation for Fatigue Life of the Spider from a Drum Washing Machine (드럼 세탁기 Spider의 피로수명 신뢰성 평가에 대한 연구)

  • 이성민;조상봉;조성진;김영수;강동우;정연수;정보선
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2004.10a
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    • pp.46-49
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    • 2004
  • The spider of a drum washing machine receives the repeated fatigue loadings during laundering. Although the spider is designed statically safely, it often happens fatigue failure. Therefore it requires the safe design for fatigue and needs the prediction of quantitative fatigue life. The S-N diagram for a spider material is developed by fatigue test and statistical analysis. The stresses are measured directly from strain gages on the spider. To predict the fatigue life of spider, the rainflow counting method and Miner's rule are used. The data for fatigue life are analyzed statistically. From these data, reliability estimation for fatigue life can be done and also, equivalent fatigue life can be obtained. It will be applied to make and improve to a short period for design and prototype test.

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An Accelerated Life Test of Booster Pump for Water Purifier (정수기용 부스터펌프의 가속수명시험)

  • Moon, Ji-Seob;Jeong, Seon-Yong;Kim, Myung-Soo
    • Journal of Applied Reliability
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    • v.11 no.3
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    • pp.281-291
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    • 2011
  • This paper presents an accelerated life test of booster pump for home water purifier. The failure analysis shows that decreased flux due to the plastic deformation of bypass spring adjusting pressure is the predominant failure mechanism. An accelerated life test is designed and implemented to estimate the lifetime of the booster pump. Temperature, water pressure and voltage are selected as accelerating variables through the technical review about failure mechanism. It is assumed that the lifetimes of booster pumps follow lognormal distribution and the combination model of temperature and non-thermal stresses holds. The life-stress relationship, acceleration factor, and $B_{10}$ life at design condition are estimated by analyzing the accelerated life test data.

Mechanical Life Prediction of a Relay by Accelerated Life Tests (가속시험에 의한 릴레이의 기계적 수명평가에 관한 연구)

  • Kwon Young-Il;Han In-Su
    • Proceedings of the Korean Reliability Society Conference
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    • 2005.06a
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    • pp.75-82
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    • 2005
  • In this paper, accelerated life testing(ALT) method and procedures for a are developed and applied to assess the reliability of the product. Relay is a device that can open and close the electric circuit electrically and is used for protecting and controlling the load. In this study, an accelerated life test method for predicting the mechanical life of a relay is developed using the relationship between stresses, failure mechanism and life characteristics of products. Using the ALT method, we performed life tests and analyzed the tests results. The proposed method and procedures may de extended and applied to testing similar kinds of products to reduce test times and costs of the tests remarkably.

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Accelerated Life Test for Door Switch (도어스위치의 가속수명시험)

  • Kim Sang Uk;Jang Young Kee;Moon Chul Hui
    • Proceedings of the Korean Reliability Society Conference
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    • 2005.06a
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    • pp.327-337
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    • 2005
  • Accelerated life test models and procedure are developed to assess the reliability of Door switch. The main function of door switch is to operate bulb lamp and fan motor. The accelerated life test method and test equipments are developed using the relationship between stresses and life characteristics of the products. Using the developed accelerated life test method, the parameters of the ALT model and life time distribution are estimated and the reliability of the Door S/W at use condition if assessed. The proposed accelerated life test method and procedure may be extended and applied to testing similar kinds of products to reduce test time and costs of the tests remarkably.

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