• 한국음향학회지
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• 제23권8호
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• pp.554-561
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• 2004

반도체 기술과 멀티미디어 통신기술이 발달하면서 고품위 영상과 다중 채널의 오디오에 관심을 갖게 되었다. MPEG 오디오 계층 3 디코더는 표준안에 기반을 둔 프로세서로써 기존에 많이 구현되어 있다. MPBG-1오디오 계층3 디코더의 합성필터는 디코더 전체에서 가장 많은 연산을 필요로 하기 때문에 고속 프로세서를 설계하기 위해서는 연산량을 줄일 수 있는 새로운 방식의 합성필터를 필요로 한다. 따라서 본 논문에서는 MPEG-1 오디오 계층 3의 핵심부분인 합성필터 부분을 DALUT (distributed arithmetic look-up table)방식을 이용하여 FPGA (Field Programmable Gate Array)에 구현하였다. 고속 필터를 설계하기 위해서 승산기 대신에 DALUT방식을 사용하였고, 파이프라인 구조를 사용하였으며, 데이터를 코사인 함수와 곱셈한 결과를 테이블로 만듦으로써 곱셈기를 제거하여 30%의 성능향상을 얻었다. 본 논문에서의 하드웨어 설계는 모두 VHDL (VHSIC Hardware Description Language)로 기술하였다. VHDL 시뮬레이션은 ALDEC사의 Active-HDL 6.1과 Model-sim 및 합성은 Synplify Pro 7.2v을 사용하였다. 대상 라이브러리는 XILINX사의 XC4010E, XC4020BX, XC4052 XL, P&R 툴은 XACT Ml.4를 사용하여 구현하였다. 구현된 프로세서는 20MHz∼70MHz사이에서 동작한다.

• 대한의생명과학회지
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• 제13권4호
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• pp.263-272
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• 2007

Nebulin is a giant actin binding protein (600-900 kDa) which is specific to skeletal muscle. This protein is known to regulate thin filaments length in sarcomere as a molecular template. The C-terminus of nebulin is located in the Z-disc of muscle sarcomere and is bound to other proteins such like myopalladin, titin, archvillin, and desmin. The N-terminus of nebulin binds to tropomodulin at the pointed ends of the thin filaments. In recent research, nebulin not only found in brain but also expressed in heart, stomach, and liver. So, the roles of nebulin in non-muscle tissue have been studied. However, lack of information or studies on nebulin binding proteins and nebulin function in brain are available so far. Therefore, the current study have investigated a novel binding partner of Nebulin C-terminus by using yeast two-hybrid screening with human brain cDNA library. Nebulin C-terminus, containing simple repeats, serine rich and SH3 domain, interacts with osteonectin C-terminal region. The specific interaction of nebulin and osteonectin were confirmed in vitro by using GST pull-down assay and reconfirmed in vivo by using transfected COS-7 cells with EGFP-tagged nebulin and DsRed-tagged osteonectin. Consequently, this study identified SH3 domain in nebulin C-terminus specifically binds to extracellular Ca-binding (EeC domain in osteonectin. Also, nebulin C-terminus fusion protein colocalized with osteonectin EC domain fusion protein in transfected COS-7 cells. The current study found the interaction between nebulin and osteonectin in human brain for the first time and suggested the nebulin in brain may be associated with osteonectin, as a regulator of cell cycle progression and mitosis.

• BMB Reports
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• 제37권6호
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• pp.741-748
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• 2004

The hepatitis C virus is associated with the development of liver cirrhosis and hepatocellular carcinomas. Among the 10 polyproteins produced by the virus, no function has been clearly assigned to the non-structural 5A (NS5A) protein. This study was designed to identify the hepatocellular proteins that interact with NS5A of the HCV. Yeast two-hybrid experiments were performed with a human liver cDNA prey-library, using five different NS5A derivatives as baits, the full-length NS5A (NS5A-F, amino acid (aa) 1~447) and its four different derivatives, denoted as NS5A-A (aa 1~150), -B (aa 1~300), -C (aa 300~447) and D (aa 150~447). NS5A-F, NS5A-B and NS5A-C gave two, two and 10 candidate clones, respectively, including an AHNAK-related protein, the secreted frizzled-related protein 4 (SFRP4), the N-myc downstream regulated gene 1 (NDRG1), the cellular retinoic acid binding protein 1 (CRABP-1), ferritin heavy chain (FTH1), translokin, tumor-associated calcium signal transducer 2 (TACSTD2), phosphatidylinositol 4-kinase (PI4K) and $centaurin{\delta}$ 2 ($CENT{\delta}2$). However, NS5A-A produced no candidates and NS5A-D was not suitable as bait due to transcriptional activity. Based on an in vitro binding assay, CRABP-1, PI4K, $CENT{\delta}2$ and two unknown fusion proteins with maltose binding protein (MBP), were confirmed to interact with the glutathione S-transferase (GST)/NS5A fusion protein. Furthermore, the interactions of CRABP-1, PI4K and $CENT{\delta}2$ were not related to the PXXP motif (class II), as judged by a domain analysis. While their biological relevance is under investigation, the results contribute to a better understanding of the possible role of NS5A in hepatocellular signaling pathways.

• Journal of Microbiology and Biotechnology
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• 제20권3호
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• pp.518-524
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• 2010

A mannanase gene (man26B) was obtained from a sea bacterium, Paenibacillus sp. BME-14, through the constructed genomic library and inverse PCR. The gene of man26B had an open reading frame of 1,428 bp that encoded a peptide of 475- amino acid residues with a calculated molecular mass of 53 kDa. Man26B possessed two domains, a carbohydrate binding module (CBM) belonging to family 6 and a family 26 catalytic domain (CD) of glycosyl hydrolases, which showed the highest homology to Cel44C of P. polymyxa (60% identity). The optimum pH and temperature for enzymatic activity of Man26B were 4.5 and $60^{\circ}C$, respectively. The activity of Man26B was not affected by $Mg^{2+}$ and $Co^{2+}$, but was inhibited by $Hg^{2+},\;Ca^{2+},\;Cu^{2+},\;Mn^{2+},\;K^+,\;Na^+$, and $\beta$-mercaptoethanol, and slightly enhanced by $Pb^{2+}$ and $Zn^{2+}$. EDTA did not affect the activity of Man26B, which indicates that it does not require divalent ions to function. Man26B showed a high specific activity for LBG and konjac glucomannan, with $K_m,\;V_{max}$, and $k_{cat}$ values of 3.80 mg/ml, 91.70 ${\mu}mol$/min/mg protein, and 77.08/s, respectively, being observed when LBG was the substrate. Furthermore, deletion of the CBM6 domain increased the enzyme stability while enabling it to retain 80% and 60% of its initial activity after treatment at $80^{\circ}C$ and $90^{\circ}C$ for 30 min, respectively. This finding will be useful in industrial applications of Man26B, because of the harsh circumstances associated with such processes.

• Molecules and Cells
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• 제25권4호
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• pp.559-565
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• 2008

Members of the TGA family of basic domain/leucine zipper transcription factors regulate defense genes through physical interaction with NON-EXPRESSOR OF PR1 (NPR1). Of the seven TGA family members, TGA4/octopine synthase (ocs)-element-binding factor 4 (OBF4) is the least understood. Here we present evidence for a novel function of OBF4 as a regulator of flowering. We identified CONSTANS (CO), a positive regulator of floral induction, as an OBF4-interacting protein, in a yeast two-hybrid library screen. OBF4 interacts with the B-box region of CO. The abundance of OBF4 mRNA cycles with a 24 h rhythm under both long-day (LD) and short-day (SD) conditions, with significantly higher levels during the night than during the day. Electrophoretic mobility shift assays revealed that OBF4 binds to the promoter of the FLOWERING LOCUS T (FT) gene, a direct target of CO. We also found that, like CO and FT, an OBF4:GUS construct was prominently expressed in the vascular tissues of leaf, indicating that OBF4 can regulate FT expression through the formation of a protein complex with CO. Taken together, our results suggest that OBF4 may act as a link between defense responses and flowering.

• 한국초등수학교육학회지
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• 제12권2호
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• pp.149-163
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• 2008

본 연구는 1998년부터 2006년까지의 초등수학교육 관련 학술지 논문의 연구 동향을 분석하여 현재까지의 학술지의 논문이 분야별로 얼마나 고르게 연구되었으며, 제7차 교육과정에 대한 기대와 요구에 부합되게 초등수학교육연구가 진행되고 있는지에 대해 알아보고, 나아가서 향후 초등수학교육 연구의 발전 방향을 모색하기 위하여 수행되었다. 이를 위하여 먼저 이론적 고찰 및 선행연구를 통해 연구연도, 연구주제, 수학과 내용영역, 연구방법, 연구대상, 연구지역 등을 분석기준으로 삼고, 분석대상으로 국회전자도서관에서 검색항목 중 키워드(key word)를 '초등 수학'으로 하여 검색된 학술지 논문 중에서 1998년 1월부터 2006년 12월까지 발표된 국내수학교육 전문 학술지인 대한수학교육학회, 한국수학교육학회, 각 교육대학교 논문집에 게재된 논문 175편과 한국초등수학교육학회 학술지 논문 60편을 수집하여 내용분석을 실시하였다. 그 결과 초등수학교육에 관한 연구는 지속적으로 이루어지고 있으나 연구주제 및 수학과 내용영역에서는 수업설계와 방법, 수와 연산 등 특정영역에 편중되게 연구가 이루어지고 있음을 알 수 있었다. 또한 연구방법에서는 초등수학교육 이론정립 및 내용분석과 관련된 교수학적분석연구가 높은 빈도를 나타내고 있어 연구의 균형적인 발전을 위하여 개별적인 특성과 환경적 변인 등을 고려한 보다 다양한 질적 연구의 필요성을 인지할 수 있었다.

• 생명과학회지
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• 제14권2호
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• pp.325-330
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• 2004

효모에 있어 자외선에 의한 절제회복 관여 DNA회복유전자가 많이 알려져 있으나, 이들이 어떤 기능을 하는지는 아직 잘 알려져 있지 않다. 본 연구에서는 자외선 조사 시 절제회복의 초기 단계에 절대적으로 필요한 RAD3 유전자와 유사한 유전자인 HRD3 유전자를 분열형 효모인 Schizosaccharomyces pombe에서 분리하여 그 특성을 연구하였다. 이 결과 분리한 유전자는 효모 RAD3 유전자와 염기서열에서 약 70%이상의 유사성을 보였다. 이 유전자의 염기서열 결과 유전자 산물의 분자량은 75 kDa였다. 2-D gel 결과 과잉발현 시 HRD3 단백질은 숙주 단백질의 합성 억제 또는 분해 촉진을 유발하여 숙주세포인 대장균에 독성초과를 나타내었다. HRD3 유전자와 lacZ 유전자를 융합시킨 여러 가지 재조합 vector를 만들어 이들 융합단백질을 분리, 연구 한 결과 HRD3 단백질의 카르복실 말단부분이 효모에 있어서 DNA회복기능과 대장균에서의 독성효과를 나타내는 중요부위임이 확인되었다.

• 융합신호처리학회논문지
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• 제11권2호
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• pp.177-182
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• 2010

시스템수준 설계방법론에서 널리 사용하고 있는 설계흐름도는 시스템명세, 시스템수준의 하드웨어/소프트웨어 분할, 하드웨어/소프트웨어 통합설계, 가상 또는 물리적 프로토타입을 이용한 통합검증, 시스템통합으로 구성된다. 시스템의 하드웨어 구성요소를 개발하는 과정에서 이전까지는 디자인단계가 많은 시간 및 노력을 요구하는 단계였지만, 현재에는 설계한 디자인의 기능적 검증단계가 중요 요소로 간주되고 있다. 본 논문에서는 시스템수준 설계언어인 SystemC 기반의 테스트벤치 구조를 이용하여 Verilog HDL로 설계된 하드웨어 구성요소의 올바른 동작여부를 판별하는 기능검증시스템을 설계하였다. 설계된 기능검증시스템에서 SystemC 모듈의 멤버 변수와 Verilog 모듈의 와이어 및 레지스터 변수간의 데이터 전달은 본 논문에서 정의되는 SystemC 사용자 정의 통신채널을 통하여 이루어진다. 제안된 기능검증시스템을 UART에 적용하여 올바른 동작여부를 판별하였다. 본 논문의 기능검증시스템 설계에 사용된 SystemC는 C++기반의 하드웨어 모델링용 클래스 라이브러리를 제공하므로 RT 수준보다 높은 추상화수준에서 소프트웨어와 하드웨어 또는 이 둘을 결합한 시스템수준의 모델링을 단일 언어와 환경에서 설계할 수 있는 이점이 있다. 또한 기능검증시스템 설계에 작성된 SystemC 모듈 코드들은 부분적인 코드 수정 후 다른 하드웨어 구성요소의 기능을 검증하는데 재사용할 수 있는 이점이 있다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.13-13
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• 2014

Species belonging to the genus Fusarium are widely distributed and cause diseases in many plants. Isolation of fungal strains from air or cereals is necessary for disease forecasting, disease diagnosis, and population genetics [1]. Previously we showed that Fusarium species are resistant to toxoflavin produced by the bacterial rice pathogen Burkholderia glumae while other fungal genera are sensitive to the toxin, resulting in the development of a selective medium for Fusarium species using toxoflavin [2]. In this study, we have tried to elucidate the resistant mechanism of F. graminearum against toxoflavin and interaction between the two pathogens in nature. To test whether B. glumae affects the development of F. graminearum, the wild-type F. graminearum strains were incubated with either the bacterial strain or supernatant of the bacterial culture. Both conditions increased the conidial production five times more than when the fungus was incubated alone. While co-incubation resulted in dramatic increase of conidial production, conidia germination delayed by either the bacterial strain or supernatant. These results suggest that certain factors produced by B. glumae induce conidial production and delay conidial germination in F. graminearum. To identify genes related to toxoflavin resistance in F. graminearum, we screened the transcriptional factor mutant library previously generated in F. graminearum [3] and identified one mutant that is sensitive to toxoflavin. We analyzed transcriptomes of the wild-type strain and the mutant strain under either absence or presence of toxoflavin through RNAseq. Expression level of total genes of 13,820 was measured by reads per kilobase per million mapped reads (RPKM). Under the criteria with more than two-fold changes, 1,440 genes were upregulated and 1,267 genes were down-regulated in wild-type strain than mutant strain in response to toxoflavin treatment. A comparison of gene expression profiling between the wild type and mutant through gene ontology analysis showed that genes related to metabolic process and oxidation-reduction process were highly enriched in the mutant strain. The data analyses will focus on elucidating the resistance mechanism of F. graminearum against toxoflavin and the interaction between the two pathogens in rice. Further evolutionary history will be traced through figuring out the gene function in populations and in other filamentous fungi.

• Molecules and Cells
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• 제20권3호
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• pp.305-314
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• 2005

Investigation of chemically synthesized inositol 1,4,5-trisphosphate [$Ins(1,4,5)P_3$] analogs has led to the isolation of a novel binding protein with a molecular size of 130 kDa, characterized as a molecule with similar domain organization to phospholipase C-${\delta}1$ (PLC-${\delta}1$) but lacking the enzymatic activity. An isoform of the molecule was subsequently identified, and these molecules have been named PRIP (PLC-related, but catalytically inactive protein), with the two isoforms named PRIP-1 and -2. Regarding its ability to bind $Ins(1,4,5)P_3$ via the pleckstrin homology domain, the involvement of PRIP-1 in $Ins(1,4,5)P_3$-mediated $Ca^{2+}$ signaling was examined using COS-1 cells overexpressing PRIP-1 and cultured neurons prepared from PRIP-1 knock-out mice. Yeast two hybrid screening of a brain cDNA library using a unique N-terminus as bait identified GABARAP ($GABA_A$ receptor associated protein) and PP1 (protein phosphatase 1), which led us to examine the possible involvement of PRIP in $GABA_A$ receptor signaling. For this purpose PRIP knock-out mice were analyzed for $GABA_A$ receptor function in relation to the action of benzodiazepines from the electrophysiological and behavioral aspects. During the course of these experiments we found that PRIP also binds to the b-subunit of $GABA_A$ receptors and PP2A (protein phosphtase 2A). Here, we summarize how PRIP is involved in $Ins(1,4,5)P_3$-mediated $Ca^{2+}$ signaling and $GABA_A$ receptor signaling based on the characteristics of binding molecules.