• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.55-55
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• 2020

This study investigated the growth and chlorophyll fluorescence reactions of three Ardisia genus grown under various indoor light intensity conditions with the aim of evaluating their suitability as indoor plants. Young seedlings of A. crispa (Thunb.) A.DC., A. pusilla DC., and A. japonica (Thunb.) Blume were used in the experiment. The plants were cultivated indoors for 10 weeks under different light intensities: 10, 50, 100, and 200 PPFD (μmol·m^-2·s^-1), and their growth was compared with that of plants cultivated in a greenhouse during the same period (mean value 236.8±20.4 PPFD at noon). Also, chlorophyll fluorescence analysis was investigated with a portable PAM fluorometer. The indoor plants were maintained at 12/12 h photoperiod, temperature at 25±1℃, and humidity at 55±3%. Irrigation frequency (once every three days) was the same for the indoors and the greenhouse. The results of growth in three Ardisia plants showed that almost all parameters except leaf number and chlorophyll content had similar levels regardless of light intensity. A. crispa and A. pusilla plants grown in 200 PPFD were investigated to have low chlorophyll contents. Meanwhile, chlorophyll fluorescence parameters differed based on light levels. In A. crispa, the Fv/Fm (0.77), DIo/RC (0.47) and Fm/Fo (4.77) parameters tended to be poor at 200 PPFD compared to those at other light intensities. Similarly, the DIo/RC, Fm/Fo, and Pi_Abs parameters of A. pusilla plant (200 PPFD) are 0.45, 4.48 and 2.42, respectively, which can be considered stress. The analysis of fluorescence in A. japonica showed that all parameters except ETo/RC had similar levels regardless of light intensity. The ETo/RC parameter was 0.49 and 0.72 in the control plants and plants 200 PPFD, respectively, which was lower than those in plants at other light intensities. Therefore, it seems that the relatively high light intensity acted as a stressor for Ardisia plants.

• 한국해양학회지
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• 제29권3호
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• pp.228-238
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• 1994

제4기 동안에 일어난 해수면 변화는 지역적 그리고 나아가 전 지구 기후변화에 좋 은 지시자가 되고 있다. 세계 해수면은 제 4기 동안 매우 불규칙하게 변해왔는데 여기 에 대한 정밀한 수학적 접근은 어려운 실정이었다. 이 연구에서 처음으로 세계 해수면 변동자료에 확율 프랙탈을 적용하여 제4기 해수면 변동을 재구성 하였다. 미국 대서양 연안에 위치한 허드슨만과 델라웨어 해안의 홀로세 해수면 변화에 대한 프랙탈 차원을 상자 계산 방법으로 측정한 결과, 허드슨만 해수면 분노는 프랙탈 차원이 1,358이고 델라웨어 해수면 변화는 1,346이다. 프랙탈 내삽함수의 역산 방법 (IFIF)의 유효성이 허드슨만과 델라웨어 해수면의 내삽에 의해서 검증되었고, IFIF에 의해서 두지역에서 의 합리적인 해수면들이 복원되었다. 델라웨어 해수면 자료는 허드슨 해수면 자료보다 IFIF의 내삽에 대해 통계적 오차가 적게 나타나는데, 이는 델라웨어 해수면 자료가 허 드슨 자료보다 더 신빙성이 높다는 것을 의미한다. IFIF는 한국 해안의 해수면을 추정 하는 데도 이용되었는데, 한국 해안의 해수면 자료는 허드슨만이나 델라웨어 지역보다 자료가 충분치 못하다. 따라서 IFIF 의 적용 통계 오차가 적은 텔라웨어 해수면 자료 에서 구한 프랙탈 차원을 사용하여 한국 해안에 대한 IFIF의 수직 비율 인자를 계산하 였다.함수(FIF)를 한국 해안과 미국 대서양 연안의 고해수면의 재구성에 적용한 결과,한국의 고해수면은 미국의 고해수면과 다른 변화를 보였다.8000년 전부터 현재까지 두지역의 해수면은 비교해 보면 한국 해수면이 미국 해수면 보다 훨씬 높았다.

• 한국환경보건학회지
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• 제47권3호
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• pp.245-258
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• 2021

Objectives: In many previous studies, endocrine disruptors (EDCs) have been found to affect delays in puberty. Various EDCs have been reported on, but there have been only limited epidemiologic studies on the effects of exposure to environmental heavy metals and pyrethroid pesticides on puberty delay. Therefore, the aim of this study was to investigate the association of exposure to environmental mercury, cadmium, and pyrethroid pesticides with delayed puberty in children based on national survey data that represents Korean children. Methods: We selected 450 children at the ages of 9-11 years old from the third Korean National Environmental Health Survey (3rd KoNEHS). The relations of urinary Hg, Cd, and 3-PBA with pubertal development were evaluated using multiple logistic regression analyses. Results: Urinary Hg levels were significantly associated with delayed puberty in boys [testicle development: OR=0.41 (95% CI: 0.20, 0.84); genitalia development: OR=0.35 (95% CI: 0.15, 0.81)]. Girls with higher Hg levels were more likely to experience delayed menarche [OR=0.23 (95% CI: 0.06, 0.90)]. We observed a significant 49% reduction in odds for menarche per increasing unit of urinary cadmium levels [OR=0.51 (95% CI: 0.24, 1.01)]. In addition, urinary 3-PBA showed a negative association with genitalia development in boys and menarche in girls [genitalia development: OR=0.73 (95% CI: 0.55, 0.96); menarche: OR=0.56 (95% CI: 0.32, 1.00)]. Conclusions: The results of this study support the hypothesis that exposure to environmental mercury, cadmium and pyrethroid pesticides may affect puberty delays. Additional evidence needs to be obtained through further prospective studies.

• Biomolecules & Therapeutics
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• 제30권2호
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• pp.203-211
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• 2022

Melanogenesis is the production of melanin from tyrosine by a series of enzyme-catalyzed reactions, in which tyrosinase and DOPA oxidase play key roles. The melanin content in the skin determines skin pigmentation. Abnormalities in skin pigmentation lead to various skin pigmentation disorders. Recent research has shown that the expression of EMP2 is much lower in melanoma than in normal melanocytes, but its role in melanogenesis has not yet been elucidated. Therefore, we investigated the role of EMP2 in the melanogenesis of MNT1 human melanoma cells. We examined TRP-1, TRP-2, and TYR expression levels during melanogenesis in MNT1 melanoma cells by gene silencing of EMP2. Western blot and RT-PCR results confirmed that the expression levels of TYR and TRP-2 were decreased when EMP2 expression was knocked down by EMP2 siRNA in MNT1 cells, and these changes were reversed when EMP2 was overexpressed. We verified the EMP2 gene was knocked out of the cell line (EMP2 CRISPR/Cas9) by using a CRISPR/Cas9 system and found that the expression levels of TRP-2 and TYR were significantly lower in the EMP2 CRISPR/Cas9 cell lines. Loss of EMP2 also reduced migration and invasion of MNT1 melanoma cells. In addition, the melanosome transfer from the melanocytes to keratinocytes in the EMP2 KO cells cocultured with keratinocytes was reduced compared to the cells in the control coculture group. In conclusion, these results suggest that EMP2 is involved in melanogenesis via the regulation of TRP-2 expression.

• 정보화연구
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• 제10권3호
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• pp.335-346
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• 2013

정보산업의 발전에 따라 다양하고 복잡한 업무 처리가 가능한 소프트웨어의 필요성이 비약적으로 증가 되었다. 이에 따라 소프트웨어 사용자들은 복잡한 처리도 가능하면서 더욱 안정적이고 유지보수가 용이한 프로그램 개발 기업을 선호하게 되었다. 이러한 현상은 소프트웨어 개발 조직의 능력을 정량적으로 평가하기 위한 연구로 이어졌고, 미국 국방부의 지원으로 능력성숙모델평가기준(CMMI)이 개발 되었다. 소프트웨어 개발 기업이 CMMI 상위 단계 인증을 받을 경우 해당 기업의 개발 프로세스와 유지보수가 우수한 것으로 평가되기 때문에 높은 단계의 인증을 받는 것은 의미있는 일로 평가된다. 본 논문에서는 국내 한 소프트웨어 개발 기업의 사례를 통해 정량적 우수단계인 4단계를 달성하기 위한 다양한 요소를 분석하였으며 국내 소프트웨어 기업이 개발 및 보수의 성숙된 프로세스를 확보하기 위한 기존 소프트웨어 개발 기업의 실제 사례분석을 통해 의미있는 요소를 찾아내고자 하였다. 특히 국내외 소프트웨어 판매 시 프로그램신뢰 부족 및 유지보수에 대한 안전장치 부족으로 인식될 수 있으므로, 이러한 사례분석을 통해 이러한 문제의 단초를 분석하고자 하였다.

• 생명과학회지
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• 제29권1호
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• pp.37-44
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• 2019

조릿대 잎은 항염, 해열, 이뇨작용 등의 약리효과를 가지고 있어 예로부터 전통의약에서 사용되어 왔다. 본 연구팀은 열수 추출한 후 남는 잔사로부터 식물화합물을 다량으로 함유한 잔사 추출물(PRE)을 제조하는 방법을 보고 바 있다. 본 연구는PRE의 고요산 혈증 저감소재로서 활용 가능성을 평가하기 위하여 수행하였다. Potassium oxonate(PO)로 유도한 고요산 혈증 생쥐 모델에서 PRE는 혈액 내의 요산, 요소 질소, 크레아틴 농도는 감소시켰고, 오줌 내의 요산과 크레아틴 농도는 증가하였다. 또한, PRE 투여한 고용산 혈증 생쥐에서 간 내 요산 농도와 xanthine oxidase 활성이 대조군에 비해 감소하였고, PRE는 PO에 의해 유도된 간 조직의 상해를 보호하였다. 이 결과는 PO로 유도된 고요산 혈증 생쥐에서 PRE는 항염증 및 세포보호 작용에 기인하는 것으로 판단된다. 부가적으로 PRE에 의한 신장조직에서 transcriptome의 반응 변화를 RNA 서열분석법으로 분석하였다. PRE는 주로 면역반응, 염증반응 및 대사과정에 관여하는 유전자의 발현에 영향을 미치는 것으로 나타났다. 본 연구 결과는 염증을 동반하는 고요산 혈증을 개선하는 소재로서의 PRE의 활용 가능성을 제시해 준다.

• 환경생물
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• 제32권4호
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• pp.344-352
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• 2014

본 연구에서는 암모니아 노출에 따른 조피볼락의 생리학적 반응을 조사하고자 아가미 $Na^+/K^+$-ATPase (NKA) 활성을 비롯한 혈장 parameters를 분석하였다. 실험구의 암모니아 농도는 대조구(자연해수), 1, 2, 4, $8mg\;L^{-1}$였으며, 조피볼락을 각각의 실험구에 3시간동안 노출한 다음 혈액 및 아가미 조직을 샘플링하였다. 실험구의 암모니아 농도가 높아질수록 혈장 암모니아 농도가 증가하였으며, 아가미 NKA 활성 또한 증가하는 경향을 보였다. 혈장 $Cl^-$를 제외한 외부의 암모니아 영향을 받지 않았으나, 4, $8mg\;L^{-1}$구의 $Na^+$, $K^+$ 및 삼투질농도는 대조구 및 1, $2mg\;L^{-1}$구보다 유의하게 높았다. 암모니아 노출에 따른 조피볼락의 혈장 코티졸은 암모니아 농도와 선형관계를 보였으며, 혈장 글루코스 또한 코티졸과 동반상승하는 것으로 나타났다. 1, $2mg\;L^{-1}$구의 hematocrit는 대조구와 차이를 보이지 않았으나, 4, $8mg\;L^{-1}$구는 나머지 실험구보다 유의하게 높았다. 암모니아 농도가 높을수록 아가미 조직 손상은 심하였으며, 특히 4, $8mg\;L^{-1}$구에서는 상피세포의 과증식, 분리, 괴사 및 2차새변의 곤봉화(club-shaped lamella) 현상이 관찰되었다.

• Asian Journal of Business Environment
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• 제3권2호
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• pp.17-22
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• 2013

Purpose - This dissertation is based on previous research, and analyzes processing trade, which constitutes a major section of foreign trade in Shandong Province. Research design, data, and methodology - The study uses the survey data on polarization, which is a vital index reflecting the unbalanced growth of regional economic development. The article introduces the processing trade polarization index, and the processing trade polarization fluctuation rate, to predict the geographical polarization posture and development trends in Shandong Province. Results -The development of processing trade in Shandong Province shows the level of gradient from east to west. The first-line growth pole has been formed and developed, and the initial formation of the diffusion mechanism has taken place. However, coordination problems in accompanying regional development have become increasingly prominent. Conclusions - This study focuses on the development of processing trade strategy and suggests overall coordination of development objectives, using non-balanced development goals. According to regional characteristics and development objectives of the processing trade in Shandong Province, the region around the city is divided into innovation diffusion region, enhanced growth areas, areas expected to undertake development, and areas to upgrade in four levels, given the different policy proposals.

• Nutrition Research and Practice
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• 제16권1호
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• pp.14-32
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• 2022

BACKGROUND/OBJECTIVES: Peroxisome proliferator-activated receptor-gamma co-activator-1α (PGC-1α) has a central role in regulating muscle differentiation and mitochondrial metabolism. PGC-1α stimulates muscle growth and muscle fiber remodeling, concomitantly regulating lactate and lipid metabolism and promoting oxidative metabolism. Gynostemma pentaphyllum (Thumb.) has been widely employed as a traditional herbal medicine and possesses antioxidant, anti-obesity, anti-inflammatory, hypolipemic, hypoglycemic, and anticancer properties. We investigated whether G. pentaphyllum extract (GPE) and its active compound, gypenoside L (GL), affect muscle differentiation and mitochondrial metabolism via activation of the PGC-1α pathway in murine C2C12 myoblast cells. MATERIALS/METHODS: C2C12 cells were treated with GPE and GL, and quantitative reverse transcription polymerase chain reaction and western blot were used to analyze the mRNA and protein expression levels. Myh1 was determined using immunocytochemistry. Mitochondrial reactive oxygen species generation was measured using the 2'7'-dichlorofluorescein diacetate assay. RESULTS: GPE and GL promoted the differentiation of myoblasts into myotubes and elevated mRNA and protein expression levels of Myh1 (type IIx). GPE and GL also significantly increased the mRNA expression levels of the PGC-1α gene (Ppargc1a), lactate metabolism-regulatory genes (Esrra and Mct1), adipocyte-browning gene fibronectin type III domain-containing 5 gene (Fndc5), glycogen synthase gene (Gys), and lipid metabolism gene carnitine palmitoyltransferase 1b gene (Cpt1b). Moreover, GPE and GL induced the phosphorylation of AMP-activated protein kinase, p38, sirtuin1, and deacetylated PGC-1α. We also observed that treatment with GPE and GL significantly stimulated the expression of genes associated with the anti-oxidative stress response, such as Ucp2, Ucp3, Nrf2, and Sod2. CONCLUSIONS: The results indicated that GPE and GL enhance exercise performance by promoting myotube differentiation and mitochondrial metabolism through the upregulation of PGC-1α in C2C12 skeletal muscle.

• 한국토양비료학회지
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• 제48권6호
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• pp.618-627
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• 2015

Soil salinity is the most critical factor for crop production at reclaimed tidal saline soil. Subsurface drainage system is recognized as a powerful tool for the process of desalinization in saline soil. The objective of this study was to investigate the effects of subsurface drainage systems on soil salinity and corn development at Saemangeum reclaimed tidal saline soil. The field experiments were carried out between 2012 and 2014 at Saemangeum reclaimed tidal land, Buan, Korea. Subsurface drainage was installed with four treatments: 1) drain spacing of 5 m, 2) drain spacing 10 m, 3) double layer with drain spacing 5 m and 10 m, and 4) the control without any treatment. The levels of water table showed shorter periods above 60 cm levels with the deeper installation of subsurface drainage system. Water soluble cations were significantly greater than exchangeable forms and soluble Na contents, especially in surface layer, were greatly reduced with the installation of subsurface drainage system. Subsurface drainage system improved biomass yield of corn and withering rate. Thus, the biomass yield of corn was improved and the shoot growth was more affected by salinity than was the root growth. The efficiency of double layer was not significant compared with the drain spacing of 5 m. The economic return to growers at reclaimed tidal saline soil was the greatest by the subsurface drainage system with 5 m drain spacing. Our results demonstrated that the installation of subsurface drainage system with drain space of 5 m spacing would be a best management practice to control soil salinity and corn development at Saemangeum reclaimed tidal saline soil.