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Microbial Removal Effects of Electrolyzed Acid Water on Lettuce by Washing Methods and Quality Changes during Storage (전해산화수에 의한 상치의 세척방법별 제균효과와 저장중 품질변화)

  • Jeong, Seong-Weon;Jeong, Jin-Woong;Park, Kee-Jai
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1511-1517
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    • 1999
  • Effects of washing methods using electrolyzed acid water on lettuce(Lactuca sativa) and quality changes during storage were investigated. The multi-stage immersion treated 3 times in 2 min showed more effective than others to remove microorganisms. Total count of lettuce after immersion in electrolyzed acid water was decreased to 1/100-1/300 of $5.8\;{\times}\;l0^5CFU/g$ of non-immersed lettuce and $2.3\;{\times}\;l0^5CFU/g$ of tap water immersed lettuce. Also coliforms was significantly decreased to 1/3,000 of $3.1\;{\times}\;10^3CFU/g$ after electrolyzed acid water washing. However, microbial levels of electrolyzed acid water treated one became to be similar to those of non-treated lettuce after 3 days of storage at $10^{\circ}C$. The color values of L and b of lettuce treated with electrolyzed acid water were somewhat higher than those of others. Though chlorophyll content of lettuce just after immersion in electrolyzed acid water was 9% lower than those of non-treated one, the content was decreased to the same level of other treatments during storage. Decaying ratio showed the lowest value in lettuce immersed in electrolyzed acid water until 6 days of storage. The sensory tests for overall acceptability and appearance of lettuce immersed in electrolyzed acid water showed higher than those of others until $3{\sim}6$ days of storage.

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Study on Phenolic Compounds in Lettuce Samples Cultivated from Korea Using UPLC-DAD-QToF/MS (국내 재배 상추로부터 UPLC-DAD-QToF/MS를 이용한 페놀화합물 성분 비교 연구)

  • Kim, Heon-Woong;Lee, Seon-Hye;Asamenew, Gelila;Lee, Min-Ki;Lee, Suji;Park, Jin Ju;Choi, Youngmin;Lee, Sang Hoon
    • The Korean Journal of Food And Nutrition
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    • v.32 no.6
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    • pp.717-729
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    • 2019
  • The chemical informs about 70 individual phenolic compounds were constructed from various lettuce samples based on literature sources and analytical data. A total of 30 phenolic compounds including quercetin 3-O-glucuronide, quercetin 3-O-(6''-O- malonyl) glucoside, cyanidin 3-O-(6''-O-malonyl)glucoside, chlorogenic acid and chicoric acid as major components were identified in 6 lettuce samples from Korea using UPLC-DAD-QToF/MS on the basis of constructed library. Among these, quercetin 3,7-di-O-glucoside(m/z 627 [M+H]+), quercetin 3-O-(2''-O-malonyl)glucoside(morkotin C, m/z 551 [M+H]+), quercetin 3-O-(6''- O-malonyl)glucoside methyl ester(m/z 565 [M+H]+), 5-O-cis-p-coumaroylquinic acid(m/z 339 [M+H]+) and 5-O-caffeoylquinic acid methyl ester(m/z 369 [M+H]+) were newly confirmed from the lettuce samples. In total content of phenolic compounds, 4 red lettuce samples(2,947.7~7,535.6 mg/100 g, dry weight) showed higher than green lettuce(2,687.3 mg) and head lettuce(320.1 mg).

Rapid and Sensitive Detection of Lettuce Necrotic Yellows Virus and Cucumber Mosaic Virus Infecting Lettuce (Lactuca sativa L.) by Reverse Transcription Loop-Mediated Isothermal Amplification

  • Zhang, Yubao;Xie, Zhongkui;Fletcher, John D;Wang, Yajun;Wang, Ruoyu;Guo, Zhihong;He, Yuhui
    • The Plant Pathology Journal
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    • v.36 no.1
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    • pp.76-86
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    • 2020
  • Cucumber mosaic virus (CMV) is damaging to the growth and quality of lettuce crops in Lanzhou, China. Recently, however, for the first time an isolate of lettuce necrotic yellows virus (LNYV) has been detected in lettuce crops in China, and there is concern that this virus may also pose a threat to lettuce production in China. Consequently, there is a need to develop a rapid and efficient detection method to accurately identify LNYV and CMV infections and help limit their spread. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed to detect the nucleoprotein (N) and coat protein (CP) genes of LNYV and CMV, respectively. RT-LAMP amplification products were visually assessed in reaction tubes separately using green fluorescence and gel electrophoresis. The assays successfully detected both viruses in infected plants without cross reactivity recorded from either CMV or LNYV or four other related plant viruses. Optimum LAMP reactions were conducted in betaine-free media with 6 mM Mg2+ at 65℃ for LNYV and 60℃ for 60 min for CMV, respectively. The detection limit was 3.5 pg/ml and 20 fg/ml using RT-LAMP for LNYV and CMV plasmids, respectively. Detection sensitivity for both RT-LAMP assays was greater by a factor of 100 compared to the conventional reverse transcription polymerase chain reaction assays. This rapid, specific, and sensitive technique should be more widely applied due to its low cost and minimal equipment requirements.

Modified Atmosphere Packaging of Leaf Lettuce (잎상치의 MA 포장)

  • Hong, Seok-In;Kim, Yun-Ji;Park, Noh-Hyun
    • Korean Journal of Food Science and Technology
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    • v.25 no.3
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    • pp.270-276
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    • 1993
  • Leaf lettuce (Lactuca sativa L., var. crispa) packed in $20{\mu}m$ LDPE (perforated), $20{\mu}m$ LDPE, $30{\mu}m$ HDPE, and $40{\mu}m$ LDPE pouches was stored at $4^{\circ}C,\;10^{\circ}C\;and\;20^{\circ}C$. The quality of leaf lettuce during the storage was investigated in terms of weight loss, color, soluble solids content, texture and sensory properties. Leaf lettuce exhibited the highest storage stability at $4^{\circ}C$. Shelf-life of the packed leaf lettuce was prolonged approximately $10{\sim}15$ times in comparision with that of the unpacked under all temperature conditions. Among the packed leaf lettuce, self-life in $20{\mu}m$ LDPE (perforated) and $20{\mu}m$ LDPE pouches was relatively short mainly due to discoloration and deterioration. On the other hand, the quality of the leaf lettuce packed in $30{\mu}m$ HDPE and $40{\mu}m$ LDPE pouches was kept well during the storage. Particularly, $40{\mu}m$ LDPE pouches conferred the shelf-life superior to other film pouches.

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Growth Characteristics and DPPH Radical Scavenging Activity of Lettuce 'Fidel' in Plant Factory Using Activated Mineral Groups and Light-emitting Diode Lights

  • Song, Tae-Eui;Park, Seong-Jik;Moon, Joon-Kwan;Kim, In Soo;Lee, Chang Hee
    • Korean Journal of Plant Resources
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    • v.32 no.3
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    • pp.228-236
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    • 2019
  • This study is conducted to investigate the effects of growth characteristics (GC) and DPPH radical scavenging activity (DRSA) of the heading lettuce 'Fidel' depending on four concentrations of activated mineral groups (AMG: 1.6% active minerals and 0.03% CaO) added to basic nutrient solutions (diluted by 0, 0.05, 0.1, and 0.2%) and on four different light-emitting diodes (LED; B:R:W ratio = 0:1:12, 0:1:9, 0:5:5, and 2:3:5). Both 0.1 and 0.2% AMG showed better GC of lettuce in plant width, plant length, leaf width, leaf length, and the number of leaves than those of other AMG, while leaf thickness and chlorophyll value did not show significant difference among all AMG. Moreover, 0.1 and 0.2% AMG showed heavier shoot fresh weights than those of other AMG. As for the combinations of AMG and LED, B0:R5:W5 showed the best lettuce GC regardless of AMG compared to other AMG and LED combinations. As shown in the above results of LED, although there was a difference in lettuce growth by LED, the differences of lettuce growth by AMG were statistically significant. The DRSA was the highest at 82.8% in B2:R3:W5, followed by B0:R5:W5 at 77.8%. LED showed differences but AMG did not affect DRSA. Therefore, the optimal conditions in plant factory for GC and DRSA of the lettuce were 0.1% AMG and B0:R5:W5.

Comparison of Growth According to the Seedling Methods and Freshness to Storage Ones on Lettuce (결구상추 육묘방법에 따른 생육 및 저장방법에 의한 선도 비교)

  • Lee, Jung-Soo
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.27 no.3
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    • pp.181-186
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    • 2021
  • The research was aimed to improve pre-harvest methods to maintain marketability in postharvest coniferous lettuce. Non-woven pots or plug plates were used to generate seedlings. No difference was found in growth characteristics of lettuce grown in non-woven pots vs plug plates. Since, seedling method with non-woven pots was convenient, lettuce harvested from non-woven pots used for water dipping treatment. The quality parameters, such as fresh weight loss, SPAD value, and appearance of lettuce were investigated after harvest. The lettuce with dipping treatment inside the plastic box container showed lower weight loss, higher SPAD value and better appearance compared to those exposed to the control (non-packaging) during the storage at 2℃. The treated plant showed higher SPAD and hue angle values 21.9 and 113.8°, respectively, compared to that of 18.8 and 108.3°, in non-treated plants. Therefore, it seems that the water dipping treatment is effective for storage method to maintain freshness of the lettuce. We showed the non-woven pot growing as a convenient seedling method for packaging treatment. Further studies will be continued to improve freshness postharvest of other horticultural crops.

Growth of Non-Powered Hydroponics Equipment and Quality Characteristics according to Post-Harvest Packaging by Cultural Methods on Leaf Lettuce (무동력 수경재배 장치의 상추 생육과 수확 후 포장에 따른 품질 특성)

  • Jung-Soo, Lee
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.28 no.3
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    • pp.231-236
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    • 2022
  • The research was aimed to improve pre-harvest methods to maintain marketability in postharvest leaf lettuce. In this study, the effect of hydroponics on the growth and post-harvest storage characteristics of 'Chongchima' lettuce grown in peat mass medium hydroponic system using a non-powered culture device or deep water culture (DWC) was evaluated. There was no difference in fresh weight, leaf number, SPAD, moisture content, and C/N ratio between peat moss growing medium hydroponic and DWC methods except plant height. It was found that lettuce cultivation by a nonpowered hydroponics method is easier than the existing DWC. The quality parameters, such as fresh weight loss, SPAD value, and general appearance of lettuce were investigated after harvest. There was no significant difference in fresh weight loss and general appearance during storage of lettuce by the hydroponics methods. However, with the increased storage time of SPAD, which is related to chlorophyll content, was slightly higher in peat moss medium hydroponic was than DWC. When crops such as lettuce are grown under favorable conditions without any agronomic abnormalities, it is suggested that post-harvest storage is not significantly affected by peat moss growing medium hydroponic and DWC.

Severe Root Rot on Hydroponically-Grown Lettuce Caused by Phytophthora drechsleri

  • Jee, Hyeong-Jin;Nam, Ki-Woong;Cho, Weon-Dae
    • The Plant Pathology Journal
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    • v.17 no.5
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    • pp.311-314
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    • 2001
  • Phytophthora root rot of lettuce, which has not been reported in Korea before, occurred severely in liquid hydroponic culture. The disease occurred in all seasons and was most severe in summer from June to August, showing over 90% infection rate in some farms. A total of 51 isolates collected from various farms were all identified as Phytophthora drechsleri. The fungus showed strong pathogenicity to lettuce and Chinese cabbage, moderate pathogenicity to cucurbits and tomato, and weak pathogenicity to pepper. However, the fungus was not pathogenic to other leafy vegetables namely: chicory, kale, endive, garland chrysanthemum, spinach beet, and perilla. Among 10 species of Phytophtora inoculated to lettuce, only P. drechsleri and P. cryptogea were found pathogenic.

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Characteristic of Lettuce (Lactuca sativa L.) Grown with Effective Microorganism and Bio-fertilizer (유효 미생물과 바이오 비료로 재배한 상추의 특성)

  • Kim, H.S.;Choi, S.M.;Supeno, D.;A., Okka;Chung, S.W.;Kim, H.S.;Kim, J.S.;Park, J.M.;Kwon, S.H.;Kwon, S.K.;Choi, Won Sik
    • Journal of the Korean Society of Industry Convergence
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    • v.17 no.3
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    • pp.145-150
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    • 2014
  • The purpose of this study was to evaluate the growth of lettuce last for 36 days. Lettuce planted on the same medium with different treatment. The first treatment watering using bio-fertilizer (farmer) and second watering using effective microorganism. Lettuce grown in greenhouse with temperature $18-32^{\circ}C$.Characteristic observed that growth in height and weight of the plants. From the observations that can be generated at high observation, treatment 1 had a higher than treatment 2. On the last day treatment plant with treatment 1 had a 99.8 mm high while treatment 2 has height of 90.7 mm. Observation on severe treatment 1 has a heavier weight than treatment 2. Plants with treatment 1 have a weight on the last day of 0.15665 grams while in plants with treatment 2 weights are 0.11365 grams.

Identification of Crop Growth Stage by Image Processing for Greenhouse Automation (영상정보를 이용한 자동화 온실에서의 작물 성장 상태 파악에 관한 연구)

  • 김기영;류관희;전성필
    • Journal of Biosystems Engineering
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    • v.24 no.1
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    • pp.25-30
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    • 1999
  • The effectiveness of many greenhouse environment control methodologies depends on the growth information of crops. Acquisition of the growth information of crops requires a non-invasive and continuous monitoring method. Crop growth monitoring system using digital imaging technique was developed to conduct non-destructive and intact plant growth analyses. The monitoring system automatically measures crop growth information sends an appropriate control signal to the nutrient solution supplying system. To develop the monitoring system, a linear model that explains the relationship between the fresh weight and the top projected leaf area of a lettuce plant was developed from an experiment. The monitoring system was evaluated buy successive lettuce growing experiments. Results of the experiments showed that the developed system could estimate the fresh weight of lettuce from a lettuce image by using the linear model and generate an EC control signal according to the lettuce growth stage.

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