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Effects of Various Ventilation Systems on the Carbon Dioxide Concentration and Fruiting Body Formation of King Oyster Mushroom (Pleurotus eryngii) Grown in Culture Bottles (새송이버섯 병재배에서 환기방법이 이산화탄소 농도 및 자실체형성에 미치는 영향)

  • Lee, Hyun-Uk;Ahn, Mi-Jeong;Lee, Shin-Woo;Lee, Cheol-Ho
    • Journal of Life Science
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    • v.17 no.1 s.81
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    • pp.82-90
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    • 2007
  • In an attempt to establish the appropriate ventilation device for the bottle culture of king oyster mushroom (Pleurotus eryngii), we investigated carbon dioxide concentration and fruiting body formation according to the various ventilation systems within the mushroom house. In addition to, the efficiency of air circulation and growth rate as well as the appearance of physiologically abnormal phenotypes during their growth stage were also evaluated. four different ventilation devices, parallel-pressure type, positive-pressure type, negative-pressure type, and positive- and negative-pressure type were applied in this study. The positive-and negative-pressure type showed the highest efficiency of air circulation as $CO_2$ concentration was 800 ppm and the level of air current was relatively low compared to the other types (the $CO_2$ concentration of parallel-pressure type was 1,400 ppm). Moreover, the stipe length, the cap diameter, yield, and general quality grown in positive- and negative type ventilation device were also better than in the other three devices though it took slightly longer period for harvesting (18.4 days) than the others (17.6, 17.9 and 18.3 days). The appearance of physiologically abnormal phenotypes such as fruiting body lump, soft rot, and brown rot were significantly decreased in positive-and negative type compared to other types, while the appearance rates were not much different for other symptoms of bacterial ooze, stipe limb and stipe bumpy. In summary, we propose that the optimal ventilation system for the bottle culture of king oyster mushroom is positive- and negative type, and this device is expected to increase the total quality as well as yield all year around.

CONFOCAL LASER SCANNING MICROSCOPIC MORPHOLOGY OF DENTIN-RESIN INTERFACE AND ITS RELATIONSHIP WITH SHEAR BOND STRENGTH (상아질-레진 계면의 공초점 현미경적 형태 및 전단결합강도와의 관계)

  • Choi, Nak-Won;Cho, Byeong-Hoon;Son, Ho-Hyun
    • Restorative Dentistry and Endodontics
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    • v.24 no.2
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    • pp.310-321
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    • 1999
  • In this in vitro study, confocal laser scanning microscopic morphology of dentin-resin interface and its relationship to shear bond strength were investigated after the exposed dentin surfaces were treated with 3 different kinds of dentin adhesive systems[three-step; Scotchbond Multi-Purpose Plus(SMPP), self-priming bonding resin; Single Bond(SB), self-etching primer; Clearfil Liner Bond 2(LB2)]. 52 extracted human molar teeth without caries and/or restorations. The experimental teeth were randomly divided into three groups of seventeen teeth each. In five teeth of each group, class V cavities(depth: 1.5mm) with 900 cavosurface angles were prepared at the cementoenamel junction on buccal and lingual surfaces. Bonding resins of each dentin adhesive system were mixed with rhodamine B. Primer of SMPP was mixed with fluorescein. In group 1. the exposed dentin was conditioned with etchant, applied with above primer and bonding resin of SMPP. In group 2, with etchant and self-priming bonding agent of SB. In group 3, with self-etching primer and bonding agent of LB2. After treatment with dentin adhesive systems, composite resin were applied and photocured. The experimental teeth were cut longitudinally through the center line of restoration and grounded so that about $90{\mu}m$-thick wafers of buccolingually orientated dentin were obtained. And, $70{\sim}80{\mu}m$-thick wafers sectioned horizontally, thus presenting a dentinal tubules at 900 to the cut surface of a remaining tooth, were obtained. Primer of SMPP mixed with rhodamine B was applied to these wafers. Confocal laser scanning microscopic investigations of these wafers were done within of 24 hours after treatment. To measure shear bond strength, the remaining twelve teeth of each group were grounded horizontally below the dentinoenamel junction, so that no enamel remained. After applying dentin adhesive systems on the dentin surface, composite was applied in the shape of cylinder. The cylinder was 5mm in diameter, and 2mm in thickness. Shear bond strength was measured using Instron with a cross-head speed of 0.5mm/min. It was concluded as follows ; 1. Hybrid layer of SMPP(mean: $4.56{\mu}m$) was thicker than that of any other groups. This value was not statistically significant thicker than that of SB(mean: $3.41{\mu}m$, p>0.05), and significant thicker than that of LB2(mean: $1.56{\mu}m$, p<0.05). There was a statistical difference between SB and LB2(p<0.05). 2. Although there were variations in the length of resin tag even in a sample, and in a group, most samples in SMPP and SB showed resin tags extending above $20{\mu}m$. But samples in LB2 showed resin tags of $10{\mu}m$ at best. 3. Besides primer's infiltration into demineralized peritubular dentin and dentinal tubules, fluorophore of primer was detected in the lateral branches of dentinal tubules. 4. All groups demonstrated statistically significant differences from one another(p<0.05), with shear bond strengths given in descending order as follows: SMPP(18.3MPa), SB(16.0MPa) and LB2(12.4MPa). 5. LB2 having thinnest hybrid layer($1.56{\mu}m$) showed the lowest shear bond strength(12.4MPa).

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Egg Development and Morphological Change of Larvae and Juveniles of the Starry Flounder, Platichthys stellatus (강도다리, Platichthys stellatus의 난발생과 자치어의 형태발달)

  • Byun, Soon-Gyu;Lee, Bae-Ik;Lee, Jong-Ha;Ku, Hak-Dong;Park, Sang-Un;Yun, Seong-Min;Hwang, Seon-Young;Kim, Yi-Cheong;Han, Hyung-Gyun
    • Korean Journal of Ichthyology
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    • v.19 no.4
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    • pp.350-359
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    • 2007
  • The egg development and morphological change of larvae and juveniles of the starry flounder, Platichthys stellatus were observed in laboratory. Fertilized eggs of the species, 1.09~1.19 mm (mean $1.13{\pm}0.03mm$, n=50) in diameter, were floating, colorless, transparent in shape and lacked in oil globules. The eggs hatched out 121 hours after fertilization at water temperature $8.2{\sim}11.2^{\circ}C$. The size of the hatched larvae were 2.58~2.89 mm (mean $2.67{\pm}0.09mm$) in total length, their mouth and anus were not open yet and myotome number was 14+27=41. Melanophore and xanthophore appeared on the notochord and digestive organ and the margin of membrane fin, on the yolk sac and eyes were lacking in pigment cells. 5 days after hatching the larvae attained 4.30~4.97 mm (mean$4.74{\pm}0.21mm$) in TL, and their mouth and anus were open. 10 days after hatching the larvae transformed to postlarval stage and they were 4.67~5.75 mm in TL (mean $5.30{\pm}0.31mm$), and absorbing the yolk completely. Feeding activity increased as the mouth became larger. At 23 days, the larvae attained 6.69~8.82 mm in TL (mean $7.85{\pm}0.75mm$), and the right eye was started moving to the left side of the head. At 52 days, the juveniles attained 10.99~17.06 mm in TL (mean $13.50{\pm}1.67mm$). The right eye was moved completely onto the left side. All of the fins had completed set of the fin rays (D. 64~67: A. 45~51: P. 11: V. 6: C. 19).

Influence of Heavy Metal Contents in Soils Near Old Zinc-Mining Sites on the Growth of Corn (아연광산 인근 토양중의 중금속 함량이 옥수수 생육에 미치는 영향)

  • Lee, Jong-Pal;Park, No-Kwuan;Kim, Bok-Jin
    • Korean Journal of Environmental Agriculture
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    • v.13 no.3
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    • pp.241-250
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    • 1994
  • This research was carried out to investigate how the growth of corn was affected by the heavy metal contents in soils near the old zinc-mining sites, by analyzing correlation between the growth of corn and heavy metal contents in soils collected from Yonghari Ilwoulmyun YongyangGun in Kyeongpook province in 1993. The results obtained were summarized as follows. 1. The contents of heavy metals such as Zn, Cu, Pb, Cd, and as in the Youngyang area were very high compared with those in a normal area. Heavy metal contents in soils collected from $2.0{\sim}2.5Km$ distance from the mining area were the highest, and those from 3.0 Km than those from 1.5 Km were even higher. 2. For heavy metal contents in leaves of all surveyed crops, Zn, Pb, Cu and As were the highest in soybean, followed by corn and rice. 3. Growth parameters of corn in polluted fields were comparatively poor and heavy metal contents in soils of the respective sites were higher than those in fields where rice was cultivated 8 years ago and irrigation was not done previously. 4. Heavy metal contents in the leaf part of corn plant showed a similar tendency to those in soils, being the highest among the different parts of corn plant, and they were in the decreasing order of Zn > Cu > As > Cd > Pb in each part. But the differences of metal contents in each part varied. 5. Generally, a negative relationship existed between the growth of corn and heavy metal contents in soil, of which Cu and Pb were significantly correlated with plant height, ear height, diameter of stem, ear length and yield of corn. 6. There existed a positive correlation between the contents of Pb,Cd and As in soils and those in the different parts of corn plant. The higher contents of Pb, Cd, and As in soil, the more those in corn plant incressed. The contents of Pb and As in corn grains showed a highly significant positive correlation with Cd and As contents in soils.

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Analysis of growth environment for precision cultivation management of the oyster mushroom 'Suhan' (병재배 느타리버섯 '수한'의 정밀재배관리를 위한 생육환경 분석)

  • Lee, Chan-Jung;Lee, Sung-Hyeon;Lee, Eun-Ji;Park, Hae-sung;Kong, Won-Sik
    • Journal of Mushroom
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    • v.16 no.3
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    • pp.155-161
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    • 2018
  • In this study, we analyze the growth environment using smart farm technology in order to develop the optimal growth model for the precision cultivation of the bottle-grown oyster mushroom 'Suhan'. Experimental farmers used $88m^2$ of bed area, 2 rows and 5 columns of shelf shape, 5 hp refrigerator, 100T of sandwich panel for insulation, 2 ultrasonic humidifiers, 12 kW of heating, and 5,000 bottles for cultivation. Data on parameters such as temperature, humidity, carbon dioxide concentration, and illumination, which directly affect mushroom growth, were collected from the environmental sensor part installed at the oyster mushroom cultivator and analyzed. It was found that the initial temperature at the time of granulation was $22^{\circ}C$ after the scraping, and the mushroom was produced and maintained at about $25^{\circ}C$ until the bottle was flipped. On fruiting body formation, mushrooms were harvested while maintaining the temperature between $13^{\circ}C$ and $15^{\circ}C$. Humidity was approximately 100% throughout the growth stage. Carbon dioxide concentration gradually increased until 3 days after the beginning of cultivation, and then increased rapidly to approximately 2,600 ppm. From the 6th day, $CO_2$ concentration was gradually decreased through ventilation and maintained at 1,000 ppm during the harvest. Light was not provided at the initial stage of oyster mushroom cultivation. On the $3^{rd}$ and $4^{th}$ day, mushrooms were irradiated by 17 lux light. Subsequently, the light intensity was increased to 115-120 lux as the growth progressed. Fruiting body characteristics of 'Suhan' cultivated in a farmhouse were as follows: Pileus diameter was 30.9 mm and thickness was 4.5 mm; stipe thickness was 11.0 mm and length was 76.0 mm; stipe and pileus hardness was 0.8 g/mm and 2.8 g/mm, respectively; L values of the stipe and pileus were 79.9 and 52.3, respectively. The fruiting body yield was 160.2 g/850 ml, and the individual weight was 12.8 g/10 unit.

Effect of Nursery Period on the Growth and Yield of Green Papaya (Carica papaya) Production under Non-Heated Greenhouse (청과용 파파야 무가온 생산시 육묘기간이 생육특성 및 수량에 미치는 영향)

  • Seong, Ki-Cheol;Kim, Chun Hwan;Jeong, Yong Bin;Lim, Chan Gyu;Moon, Doo Kyong
    • Journal of Bio-Environment Control
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    • v.25 no.3
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    • pp.212-217
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    • 2016
  • This study was conducted to investigate the effect of nursery period on growth and yield attribute of green papaya (var. Red lady). The nursery period was 3, 5, 7, 9, 11 and 13 months and the green papaya was transplanted on 15 April, 2015 in a non-heated greenhouse. The plant height, node number and fresh weight of nursery plant were increased as the nursery periods increased. The growth of green papaya with 13 months nursery period was better than those of other treatments. First harvest after transplanting was increased as the nursery periods were shorten. It took 137 days (18 August) at 13 months treatment, and 184 days (2 October) at 3 months treatment. The fruit length and diameter were smallest at 3 months treatment and there was no significant difference among other treatments. The fruit yield was also influenced by the nursery periods, the commercial yield was also increased as the nursery periods increased. The commercial yield was highest at 13 months treatment (3,172kg/10a), followed by 11 (2,247kg/10a) and 9 months treatment (2,357kg/10a). At 7 and 5 months treatment were 1,942kg/10a and 1,787kg/10a, respectively and the yield was lowest at 3 months treatment (1,443kg/10a). The commercial yield was significantly decreased under 7 months treatment. Although the harvest time of 11 months treatment was earlier than that of other treatments in non-heated greenhouse, 9 month treatment will be more recommendable for green papaya production because of operating costs.

The fracture resistance of heat pressed ceramics with wire reinforcement (금속선 강화에 따른 열 가압 도재의 파절저항)

  • Jo, Deuk-Won;Dong, Jin-Keun;Oh, Sang-Chun;Kim, Yu-Lee
    • The Journal of Korean Academy of Prosthodontics
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    • v.47 no.2
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    • pp.191-198
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    • 2009
  • Statement of problem: Ceramics have been important materials for the restoration of teeth. The demands of patients for tooth-colored restorations and the availability of various dental ceramics has driven the increased use of new types of dental ceramic materials. Improved physical properties of theses materials have expanded its use even in posterior crowns and fixed partial dentures. However, ceramic still has limitation such as low loading capability. This is critical for long-span bridge, because bridge is more subject to tensile force. Purpose: The wire reinforced ceramic was designed to increase the fracture resistance of ceramic restoration. The purpose of this study was to evaluate the fracture resistance of wire reinforced ceramic. Material and methods: Heat pressed ceramic(ingot No.200 : IPS Empress 2, Ivoclar Vivadent, Liechtenstein) and Ni-Cr wire(Alfa Aesar, Johnson Matthey Company, USA) of 0.41 mm diameter were used in this study. Five groups of twelve uniform sized ceramic specimens(width 4 mm, thickness 2 mm, length 15 mm) were fabricated. Each group had different wire arrangement. Wireless ceramic was used as control group. The experimental groups were divided according to wire number and position. One, two and three strands of wires were positioned on the longitudinal axis of specimen. In another experimental group, three strands of wires positioned on the longitudinal axis and five strands of wires positioned on the transverse axis. Three-point bending test was done with universal testing machine(Z020, Zwick, Germany) to compare the flexural modulus, flexural strength, strain at fracture and fracture toughness of each group. Fractured ceramic specimens were cross-sectioned with caborundum disc and grinded with sandpaper to observe interface between ceramic and Ni-Cr wire. The interface between ceramic and Ni-Cr wire was analyzed with scanning electron microscope(JSM-6360, JEOL, Japan) under platinum coating. Results: The results obtained were as follows: 1. The average and standard deviation in flexural modulus, flexural strength and fracture toughness showed no statistical differences between control and experimental groups. However, strain was significantly increased in wire inserted ceramics(P<.001). 2. Control group showed wedge fracture aspects across specimen, while experimental groups showed cracks across specimen. 3. Scanning electron microscopic image of cross-sectioned and longitudinally-sectioned specimens showed no gap at the interface between ceramic and Ni-Cr wire. Conclusion: The results of this study showed that wire inserted ceramics have a high strain characteristic. However, wire inserted ceramics was not enough to use at posterior area of mouth in relation to flexural modulus and flexural strength. Therefore, we need further studies.

Artificial Multiplication of the Black Soldier Fly(BSF), Hermetia illucens(Diptera: Stratmyidae) Using the Livestock Feces (가축분뇨를 이용한 동애등에 증식기술 개발)

  • Choi, Young-Cheol;Lee, Sang-Beom;Park, Kwan-Ho;Nam, Sung-Hee;Kim, Jong-Gill;Kim, Won-Tae;Choi, Ji-Young
    • Journal of Sericultural and Entomological Science
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    • v.50 no.2
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    • pp.63-70
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    • 2012
  • This study was conducted to investigated the distribution, ecological character and life cycle of Black Soldier Fly(BSF), Hermetia illucens, to determine effects of BSF on composing ability to the livestock feces. The distribution of BSF was defined in all parts of the country in Korea. Its main habitat was found to be areas near cattle sheds, feces sheds, living waste dump grounds, and food waste dump grounds. Excellent species of the bio-conversion using the livestock feces were selected out of two kinds of solider flies(Hermetia illucens and Ptecticus tenebrifer). And effects of BSF on composing ability to the livestock feces were pig feces 0.5 g, cattle feces 0.43 g and chicken feces 0.3 g per a larvae of BSF. In the examination of the ability of BSF to decompose food waste, volume of the livestock feces decreased by weight pig feces 77, cattle feces 83.62 and chicken feces 80%. Observed characteristics of BSF larvae and pupae stage were by developmental stage may be summarized as follows: size and length of larvae and pupae of BSF were higher than in pig feces and mixed sawdust treatments. In order to develop artificial indoor rearing techniques, The pupation and emergence rate were not visible the difference but important factor of the mating and egg-laying was a sun light. And it was visible the difference in mating ratio. Mating rate of H. illucens by seasonal change was the highest from May to July. Mating mostly occurred between 10 : 00 and 12 : 00 during which light intensity is high. As the egg-laying medium for artificial egg collection, calf feed and food waste were most effective. The ratio of egg-laying medium is waste food : sawdust(1 : 1) in volume. For egg-laying materials, flower foam and wood with holes were used; holes sized 3 ~ 5 mm in diameter, 7 ~ 10 mm in depth were most preferred for egg-laying.

Studies on Distribution and Ecology of Clithon retropictus (Martens, 1879) in South Korea (한국산 기수갈고둥의 분포 및 생태에 관한 연구)

  • Han, Seung Pil;Hwang, In Chul;Kwon, Soon Jik
    • Journal of Wetlands Research
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    • v.23 no.4
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    • pp.317-326
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    • 2021
  • The present study was carried out with a view to understand the distribution and ecology of Clihton retropictus (Martens, 1879), a class II endangered wildlife designated and protected by the Ministry of Environment, Republic of Korea, and to suggest the effective conservation and management measures for this species and its habit. First, the distribution of Clihton retropictus was investigated nationwide, and environmental factors such as salinity and substratum were analyzed in consideration of the ecological characteristics of this species. In addition, the individual size, density, and movement of Clihton retropictus per each district surveyed were analyzed. Due to its nature of inhabiting in the brackish water zone, the analysis showed that the salinity of this species was 0.7 ~ 16.6‰ at low tide and 1.0 ~ 17.3% at high tide. It was learned from the analysis of substratum that its composition in each river under investigation was slightly different, but most of substratum had a higher composition ratio than gravel (pebble, 16 ~ 64 mm). On the other hand, the size of each individual was checked by measuring the length of its diameter, and there was almost no change in the individual when the inhabited range of Clihton retropictus was short. However, there was tendency that the longer the habitat range, the bigger the individual size. From surveying the population density by season, it was made clear that, during the summer (July to August) and fall (September to October), the density was relatively higher while the number of individuals decreased significantly during winter (November to December) when the water temperature was lowered. In an effort to effectively establish conservation and management measures, the moving distance was measured, and it became known that, during the surveys in summer and fall when the individuals were active, the individuals moved in more distances than during the winter survey. The results of this study are expected to be utilized as basic data for establishing effective management plans such as creation of habitats for Clihton retropictus, its migration projects and selection of migration destinations which get unavoidable due to development projects.

Effect of Long Term Waterlogging on the Growth and Nutrient Contents of 'Campbell Early' and 'Kyoho' Grapevine Cultivars (장기 침수가 포도 '캠벨얼리'와 '거봉' 품종의 생육과 양분함량에 미치는 영향)

  • Kang, Seok-Beom;Lee, In-Bog;Jang, Han-Ik;Park, Jin-Myeon;Moon, Doo-Khil
    • Korean Journal of Environmental Agriculture
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    • v.27 no.2
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    • pp.178-184
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    • 2008
  • This work was carried out to investigate the effect of waterlogging on the growth and nutrient contents of 'Campbell Early' and 'Kyoho' grapevines under the vinyl house condition from June 14 to July 20, 2005. For the trial, seedlings of two-year-old grapevine were transplanted to 40 L pot with a sandy loam soil. Irrigation point of non-waterlogging(control) treatment was controlled at -40 kPa of soil water tension using tensiometer and waterlogging treatments were imposed for 35 days at the water levels of above 10 cm from the soil surface using tap water. The growth of aerial(shoot length, leaf number and stem diameter) and underground(root) parts of 'Campbell Early' and 'Kyoho' grapevines tended to be wholly reduced by waterlogging, while the growth of aerial parts were more severely impaired in 'Kyoho' than in 'Campbell Early' cultivar. The different responses for waterlogging between two grapevines seem to be related with the capacity for absorbing mineral nutrients, because nitrogen content of 'Campbell Early' cultivar leaves was significantly higher than that of 'Kyoho' cultivar although the contents of phosphorus and potassium in leaves of two grapevine cultivars were similarly declined. There was no significant different of fruit quality, such as contents of soluble solid, titratable acidity and weight of berry in 'Campbell Early' between waterlogging and control. In 'Kyoho' cultivar, however, berry weight and titratable acidity were decreased and soluble solid content was increased by waterlogging. It was assumed that waterlogging stress for grapevines promotes maturation and coloring processes of berries by stimulating maturation hormone such as ethylene. In conclusion, 'Campbell Early' cultivar seems to be more tolerable than 'Kyoho' cultivar when comparing the growth responses and nutrient contents between two grapevine cultivars under waterlogging.