• 한국초지조사료학회지
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• 제14권1호
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• pp.7-12
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• 1994

This study was undertaken to evaluate the effect of legume reseeding on legume establishment, dry matter yield, quality and botanical composition of grasses in timothy(Ph1eum prarense L.) dominated pasture at alpine area. Establishment number and percentage of legume in timothy dominated pasture were increased with spring, red clover(Tr$olium pratense L.) and gramoxone $treatment Dry matter yield of pasture was increased with fall and grazing after overseeding, but, there waq no difference in legume species. Crude protein content(%) of grasses at the first cutting was increased with red clover and paraquat (gramoxone) treatment. Based on the results, it is suggested that spring in seeding time, red clover in legume species and oveneeding after gramoxone treatment are good for effective renovation of timothy dominated pasture at alpine area.

• Journal of Plant Biology
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• 제13권2호
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• pp.11-14
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• 1970

Identification and observations of leucine aminopeptidase (LAP) and multiple molecular forms of the enzyme, isozymes, were made with a technique of starch-gel electrophoresis for various legume pollen. Plants tested other than Leguminosae demonstrated either no indication of the presence or at least tract of enzymes and the isozymes, although all legume pollen tested showed strong LAP patterns. The electrophoretic patterns of LAP failed to be shown if the extracts were heated or otherwise denatured. Extent of zymogrammatic appearance of LAP and the isozymes were characteristic of a species.

• 한국초지조사료학회지
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• 제12권1호
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• pp.71-76
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• 1992

Starch gel electrophoresis was used to examine the banding pattern of Esterase isozyme in the leaf, root-nodule and seedling of four wild legume species, Trifolim repense, Glycine soja, Phaseolus nipponensis and Vigna uexillata. The number of band, enzyme activity and migrating rate of esterase isozyme varies depending on the species and tissues of legume plants. The isozyme banding pattern in the cotyledon and radicle of T. repense showed same pattern, however, the number of band were varible among the cotyledon of G. soja, P. nipponensis and V. vexzllata, respectively. Est-1 in the leaf of G. soja, V, vexillata. root-nodule of G. soja and seedling of V. vexillata expreesed the highest enzyme activity. The Est-1 showed the rapidest migrating rate among the isozymes.

• Asian-Australasian Journal of Animal Sciences
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• 제27권6호
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• pp.825-831
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• 2014

The aim of this study was to determine the nutritive value of some legume species in salt-affected soils of South-East Anatolian region using chemical composition and in vitro gas production kinetics. In this study, Lotus corniculatus, Trifolium alexandrinum, Medicago sativa were sown and tested in four different locations. A 3 by 4 factorial design with 3 legume species and 4 salt levels (non salty electrical conductivity (EC)<4 dS/m; low salt: 4 dS/m>EC<8 dS/m, medium saline: 8 dS/m>EC<16 dS/m and high salt: 16 dS/m>EC) was used in the study. Results indicated that salinity and plants had no significant effect on ash and ether extract. Dry matter (DM), acid detergent fiber, digestible dry matter, dry matter intake (DMI) were affected by plant, salinity and plant${\times}$salinity interaction. On the other hand neutral detergent fiber, relative feed value (RFV), and DMI were affected by salinity and plant${\times}$salinity interaction. Mineral contents were affected by plant species, salinity and salinity${\times}$plants interactions. In vitro gas production, their kinetics and estimated parameters such as were not affected by salinity whereas the gas production up to 48 h, organic matter digestibility, metabolizable energy (ME), and net energy lactation ($NE_L$) were affected by plant and plant${\times}$salt interaction. Generally RFVs of all species ranged from 120 to 210 and were quite satisfactory in salty conditions. Current results show that the feed value of Medicago sativa is higher compared to Lotus corniculatus and Trifolium alexandrinum.

• 한국초지조사료학회지
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• 제11권4호
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• pp.199-202
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• 1991

Red clover, ladino clover, white clover, alfalfa, 차풀 그리고 갈키나물등 6종의 콩과식물에서 잎과 줄기의 Esterase Isozyme를 추출하여 starch gel 전기영동법으로 분리한 후 염색하여 종간 차이점을 고찰하였다. 공시된 식물의 Esterase Isozyme의 band수, 염색강도, 이동속도는 종간에 차이가 있었다. 그러나 동일종에 있어서는 alfalfa를 제외하고는 잎과 줄기사이에 band의 수는 차이가 없었으며 차풀과 갈키나물에서는 전혀 나타나지 않았다.(Fig. 2). Esterase Isozyme의 염색강도와 이동속도는 Est 1 이 가장 강하고 빨랐다.

• 한국작물학회지
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• 제31권4호
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• pp.426-433
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• 1986

목초의 단ㆍ혼파군낙에서 초종조합별 수량성과 각 초종의 지상부와 지하부의 경합력을 평가하고 그들의 상호관계를 비교하여 이상적인 혼파초지모형의 기초자료 및 방법을 제시하기 위하여 오차드그라스, 토올페스큐, 라디노클로버 및 알팔파의 각 단파와 2초종의 혼파조합으로 이루어진 10개의 대형폿트 군락에서 시험을 실시하였다. 경합관계는 지상부와 지하부를 분리하여 건물중으로 평가하였데 그 방법으로서 는 expected yield, aggressivity, relative yield total (RYT) 및 compensation index (CI)와 general combining ability (GCA) 및 specific combining ability(SCA)를 계산하여 비교ㆍ해석하였다. 목초수량에서는 화본과초종인 오차드그라스, 토올페스큐의 각 단파 및 그들의 혼파가 유리하였고 두과초종인 라디노클로버와 알팔파의 단파 및 혼파의 경우에는 불리하였다. 목초의 수량으로 살피본 지상부경합에서 토올페스큐-라디노클로버 혼파조합만이 RYT가 1.05로 유리하였고 단파의 경우보다 토올페스큐가 우점종이었다. 또한 화본과혼파와 두과혼파는 사본과-두과혼파조합보다 우열정도의 차이가 적었고 화본과초종은 두과초종에 대해 우점종이었으며 그 중 오차드그라스가 가장 우점력이 큰 초종으로 냐타딘고 알팔파는 가장 낮은 초종이었다. 한편 혼파의 경우 조합능력을 나타내는 혼생능력에서는 토올페스큐가 가장 켰으며 그 다음이 오차드그라스였고 라디노클로버는 가장 적은 것으로 나타났다. 조합별 혼생능력은 동일과간혼파조합이 불리한 것으로 나타났으며 화본과와 두과가 혼합된 조합이 불리하게 나타났는데 그 중 토올페스큐와 라디노는 혼생능력이 가장 큰 조합이었다. 지하부 경합에서도 지상부와 마찬가지로 토올페스큐-라디노혼파가 RYT가 가장 크고 혼생능력도 큰조합이었으며, 4초종중 오차드그라스가 우점력이 가장 컸고 근부의 혼생능력에서도 지상부와 달리 오차드그라스가 가장 큰 초종이었다.

• Journal of Plant Biotechnology
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• 제39권1호
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• pp.49-61
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• 2012

This review summarises the biology, discovery and applications of single nucleotide polymorphisms in complex polyploid crop genomes, with a focus on the important oilseed crop $Brassica$ $napus$. $Brassica$ $napus$ is an allotetraploid species, and along with soybean and oil palm is one of the top three most important oilseed crops globally. Current efforts are well underway to $de$ $novo$ assemble the $B.$ $napus$ genome, following the release of the related $B.$ $rapa$ 'A' genome last year. The next generation of genome sequencing, SNP discovery and analysis pipelines, and the associated challenges for this work in $B.$ $napus$, will be addressed. The biological applications of SNP technology for both evolutionary and molecular geneticists as well as plant breeders and industry are far-reaching, and will be invaluable to our understanding and advancement of the $Brassica$ crop species.

• The Plant Pathology Journal
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• 제17권2호
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• pp.67-70
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• 2001

Medicago truncatula is a diploid legume plant related to the forage crop alfalfa. Recently, it has been chosen as a model species for genomic studies due to its small genome, self-fertility, short generation time, and high transformation efficiency. M. truncatula engages in symbiosis with nitrogen-fixing soil bacterium Rhizobium meliloti. M. truncatula mutants that are defective in nodulation and developmental processes have been generated. Some of these mutants exhibited altered phenotypes in symbiotic responses such as root hair deformation, expression of nodulin genes, and calcium spiking. Thus, the genes controlling these traits are likely to encode functions that are required for Nod-factor signal transduction pathways. To facilitate genome analysis and map-based cloning of symbiotic genes, a bacterial artificial chromosome library was constructed. An efficient polymerase chain reaction-based screening of the library was devised to fasten physical mapping of specific genomic regions. As a genomics approach, comparative mapping revealed high levels of macro- and microsynteny between M. truncatula and other legume genomes. Expressed sequence tags and microarray profiles reflecting the genetic and biochemical events associated with the development and environmental interactions of M. truncatula are assembled in the databases. Together, these genomics programs will help enrich our understanding of the legume biology.

• 한국초지조사료학회지
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• 제12권3호
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• pp.153-160
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• 1992

본 시험은 고령지 두과목초의 적응성을 구명하기 위하여 표고가 다른 3개지역(600,800,1000m a.s.l)에서 주구를 파종시기(추파.추파), 세구를 혼파조명(알팔파-화본과, 레드크로바-화본과, 화이트크로바-화본과)으로 두고 분할구 배치 3반복으로 수행하였다. 1.600m에서 두과목초의 적응성을 보면 알팔파, 레드클러버 및 화이트클로버 공히 추파를 했을 때 연차간 악화없이 약 30%를 유지하여 적응성이 매우 양호하였다.2. 800m에서는 화치트클로버를 춘파하므로써 연차간에 심한 감소없이 최종 예취시 약 14.0%를 점유하고 있었으며 또한 10a당 건물수량이 가장 많았다. 3. 1000m에서도 800m와 비슷한 경향으로 화이트클로버를 춘파했을 경우 어느정도 적응이 가능하였다. 4. 표고별 두과목초의 비솔 및 수량이 표고가 증가 할수록 현저하게 감소하였으며 알팔파는 800m 이상에서는 생육이 거의 불가능하였다.

• 한국초지조사료학회지
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• 제11권3호
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• pp.158-161
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• 1991

Horizontal starch gel electrophoresis, follow by enzyrne-specific staining, separate and visualize several legume esterase isozyme. Using extracts prepared from cotyledon, radicle and plumule of legume seedlings germinated 5 days. The results were as follows. 1. The number and staining intensity of esterase isozyme bands varies depending on the plant species. tissues and developmental stage. 2. Bands in the cotyledon of field bean seedling expressed 4 and 1 in radicle. 3. In soybean cultivars, cotyledon of IIwangkum-kong had 3 bands and 1 band in the examined tissues of Paldal-kong and Jangkyung-kong seedling. 4. The cotyledon and radicle of french bean seedling had 3 bands, respectively. 5. The highest esterase isozyme activity appears to be expressed in the cotyledon and radicle of french bean, as indicated by intensity of stain, with the Paldal-kong particulary active.