• 한국식품영양과학회지
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• 제40권2호
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• pp.163-170
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• 2011

개똥쑥(Artemisia annua L.)의 기능성을 밝히고 식품으로써 이용성을 증대시키기 위하여 개똥쑥 잎과 줄기의 영양 성분 및 생리활성을 측정하였다. 수분, 조지방 및 조단백질 함량은 잎에서 높았으나 회분, 조섬유 및 무기물 함량은 줄기에서 유의적으로 높았다. 총 페놀과 플라보노이드 함량은 잎이 줄기에 비해 약 2배 정도 높았다. 추출물의 농도를 달리하여 항산화능을 측정한 결과 농도에 의존적으로 활성이 증가하였으며, 잎은 줄기에 비해 활성이 높았다. 특히, DPPH 라디칼 소거능, 환원력 및 tyrosinase 저해활성은 모든 농도에서 잎이 줄기에 비해 유의적으로 높았으나, ${\alpha}$-glucosidase 저해활성은 줄기 추출물이 더 높았다. 인체 유방암 세포인 MCF-7 및 MDA-MB-231에 대한 증식억제 활성은 줄기보다 잎 추출물에서, 물보다 에탄올 추출물에서 유의적으로 활성이 높았다. 특히, 잎 에탄올 추출물의 MCF-7 및 MDAMB-231 세포에 대한 증식억제 활성은 $250\;{\mu}g$/mL에서 각각 76.26% 및 52.59%로 추출물 중 가장 높은 활성을 보였다. 이상의 결과, 개똥쑥 추출물의 생리활성은 시료 중의 섬유소, 페놀 및 플라보노이드 함량에 의존적인 것으로 생각된다.

• Journal of Ginseng Research
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• 제38권4호
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• pp.289-292
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• 2014

Ginseng leaf/stem extract produced by subcritical water extraction at high temperature ($190^{\circ}C$) posses higher cytotoxic activity against human cancer cell lines than ethanol extract. Subcritical water extraction can be a great candidate for extraction of functional substance from ginseng leaves/stems.

• 한국식품영양과학회지
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• 제24권6호
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• pp.1016-1019
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• 1995

This study was conducted to investigate the nutrient composition of hydroponic water dropwort and the effect of blanching condition on ascorbic acid content. Nutrient composition of hydroponic water dropwort of mid harvest on the 30th day was measured in three portions of leaves, petioles and stems, and was compared with that of late harvest on the 40th day. Hydroponic water dropwort was obtained from the Department of Horticultural Science, Seoul National University. The results were as follows. The nutrients content of leaves were significantly higher in ash and ascorbic acid and lower in moisture, crude fat and crude fiber than those of stems and petioles. There was no significant difference in total vitamin A and thiamin contents between three portions. Ascorbic acid content of leaves harvested on the 30th day was the highest, 57mg/100g, and decreased to 18mg/100g in the leaves harvested on the 40th day. Marked increase in crude fiber and vitamin A content of leaves was observed in late harvest may be due to the maturation. The results of nutrient composition analysis suggest that the leaves of hydroponic water dropwort is important in ascorbic acid and ash. It is recommended that shorter blanching time and addition of 0.5% NaCl to the blanching water are better for higher ascorbic acid retention of hydroponic water dropwort. In conclusion, as hydroponic water dropwort has high content in ash, calcium, vitamin and free sugar with alkalinity, leaves as well as stems and petioles can be recommonded as a vegetable of high nutritional quality.

• Journal of Plant Biology
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• 제35권3호
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• pp.229-235
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• 1992

담배 식물체에서 Arabidopsis thaliana에 존재하는 3가지 다른 엽록소 a/b 결합 단백질 (cab) 유전자 promoter들의 발현을 조사하였다. 이들 promoter의 활성은 이들 promoter에 결합된 reporter gene의 식물인 CAT (chloramphenicol acetyltransfer-ase)의 활성으로 측정하였다. 담배엽에서 cab promoter의 활성은 cab1, cab2, cab3의 순이었고, 이들 잎에서 유도한 callus나 shoot에서도 동일한 양상을 보였다. 이들 3가지 promoter의 발현은 상부엽에서 하부엽보다 높은 활성은 보였으며 개체간의 높은 변이폭은 뿌리내리기를 통하여 무성증식된 식물체를 사용하여 줄일 수 있었다. 이들은 또한 기관 특이성을 보여 줄기 보다 잎에서의 활성이 높았고 뿌리에서는 거의 발현되지 않았다. 그러나 cab1 promoter의 경우, 다른 두 cab promoter들과는 다르게, 잎에 대한 줄기에서의 상대적인 활성이 높았으며, CAT 활성을 단위 단백질당을 표시하는 대신 단위 엽록소당 활성으로 나타내었을 때 줄기와 잎에서 활성의 차이가 관찰되지 않았다. 즉 cab2와 cab3는 광합성 기관 특이성을 보이는 반면 cab1의 경우는 이러한 특이성을 나타내지 않았다. 이러한 현상은 잎에서 유도한 shoot가 줄기와 잎으로 분화되는 과정에서도 관찰되었다.

• 동아시아식생활학회지
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• 제17권5호
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• pp.696-702
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• 2007

The purpose of this paper is to describe the differences in mineral, ascorbic acid, and total polyphenol content, and also the relationship between polyphenol content and antioxidant activity(DPPH radical scavenging, FTC, and TBA methods) in three sizes of Cudrania tricuspidata leaves. For the mineral contents of the leaves, Ca was highest(17.26 mg/g.d.w.), followed by K(15.53 mg), P(2.61 mg), and Mg(1.99 mg). Ascorbic acid was found to be slightly higher(43.4 mg/100g.f.w.) in the basal parts of the medium size leaves and lowest in the whole parts(9.9 mg) of the small size leaves. Total polyphenol content was highest($1,384{\sim}1,258mg/100g.f.w.$) in the leaves, for all sizes, when extracted with 80% ethanol, followed by the stem bark(543 mg%), roots(369 mg%), stems(243 mg%), and thorns(223 g%). By determining DPPH activity, we found that antioxidant activity was higher in the ethanol extracts of the leaves than in the ethanol extracts of the thorns, stems, roots, and stem bark. While in the tea, no differences were found between the ethanol and water extracts.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.338-344
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• 2023

Curcuma longa is a plant belonging to the genus Curcuma and is distributed across various Asian regions. This plant is widely known for its rhizomes, which possess a variety of pharmacological properties. However, although the leaves and stems of this plant also contain several health-promoting secondary metabolites, very few studies have characterized these compounds. Therefore, our study sought to quantify the secondary metabolites from the leaves and stems of Curcuma longa L. (LSCL) using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) and high-performance liquid chromatography (HPLC). Our LC-ESI-MS analyses detected twenty-one phenolic compounds in the LSCL, among which fifteen compounds were detected via HPLC analysis. Four compounds, namely vanillic acid (0.129 mg/g), p-coumaric acid (0.431 mg/g), 4-methylcatechol (0.199 mg/g), and afzelin (0.074 mg/g) were then quantified. These findings suggest that LSCL is rich in secondary metabolites and holds potential as a valuable resource for the development of functional and nutritional supplements in the future.

• 한국환경과학회지
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• 제26권11호
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• pp.1285-1295
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• 2017

Chrysanthemum boreale Makino is widely distributed in Korea, China, Japan and Southeast Asian countries. C. boreale is one of the herbs used for treating various inflammatory diseases in oriental medicine. The present study was conducted to identify biologically active compounds from the leaves and stems of C. boreale. We isolated two sesquiterpene sactones from the leaves and stems of C. boreale using silica gel column chromatography and recyclic high perfomance liquid chromatography. The lactones were characterized by their spectroscopic data (NMR, IR, MASS). These compounds were subjected to Farnesyl Protein Transferase (FPTase) inhibition, Nitric Oxide (NO) release inhibition and apoptosis inhibition. The structur of the following isolated compound were elucidated 8,10-${\small{O}$-Acetyl-2-methoxy-10-hydroxy-3,11(13)-guaiadiene-12,6-olide and 4,10-dihydroxy-8-${\small{O}$-Acetyl-2,11(13)-guaiadiene-12,6-olide. In the NO release inhibition assay, compound 2 showed strong activities, with an $IC_{50}$ value of $7{\mu}g/mL$, whereas compound 1 did not exhibit significant activity with an $IC_{50}$ value of over $14{\mu}g/mL$ against murine macrophage.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2000년도 THE THIRD INTERNATIONAL CONFERENCE ON AGRICULTURAL MACHINERY ENGINEERING. V.II
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• pp.300-306
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• 2000

This paper introduces a new concept of automation for bio-production with tele-operative system. The proposed system showed practical and feasible way of automation for the volatile bio-production process. Based on the proposition, recognition of the job environment with object identification was performed using computer vision system. A man-machine interactive hybrid decision-making, which utilized a concept of tele-operation was proposed to overcome limitations of the capability of computer in image processing and feature extraction from the complex environment image. Identifying watermelons from the outdoor scene of the cultivation field was selected to realize the proposed concept. Identifying watermelon from the camera image of the outdoor cultivation field is very difficult because of the ambiguity among stems, leaves, shades, and especially fruits covered partly by leaves or stems. The analog signal of the outdoor image was captured and transmitted wireless to the host computer by R.F module. The localized window was formed from the outdoor image by pointing to the touch screen. And then a sequence of algorithms to identify the location and size of the watermelon was performed with the local window image. The effect of the light reflectance of fruits, stems, ground, and leaves were also investigated.

• Journal of Ginseng Research
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• 제8권2호
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• pp.91-104
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• 1984

This study was conducted to investigate the characteristics of nonstarchy polysaccharides in Korean ginseng, (Panax ginseng C.A. Meyer). The results obtained are as follows. 1. The total sugar content of ginseng roots were decreased with increasing the cultural period. On the other hand, the crude fiber content was increased with that of the ginseng leaves or stems. But the crude fiber in root was much less than that of leaves and stems. 2. The dietary fiber content of ginseng root on 5 years old was 14.20% as neutral detergent fiber, 9.08% as acid detergent fiber, hemicellulose 5.12%, cellulose 7.98% and lignin of 1.10%, respectively. 3. Much more pectin was found in ginseng roots which was cultivated for shooter Period. And it was contained much more in the root than in the leaves and stems. 4. ginseng hemicellulose content in root was 5% to 10%. It was decreased with increasing: cultivated period. Hemicellulose was constituted of xylose, arabinose, glucose, rhamnose and xylose of these sugars was the predominant. 5. X-ray diffraction Pattern of ginseng cellulose showed maximum intensity at tile interplanar angle of 4.1$^{\circ}$.

• The Plant Pathology Journal
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• 제20권3호
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• pp.192-195
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• 2004

A survey on ornamental diseases in 2000 and 2001 showed frequent occurrence of blight symptoms on leaves and stems of columbine and bleeding heart in botanical gardens of Gangwon and Chungnam provinces in Korea. A total of 65 isolates of Streptobotrys sp. were obtained from the infected leaves and stems of the two plants. All isolates were identified as Streptobotrys caulophylli based on their morphological and cultural characteristics. Blight symptoms were induced on leaves of the two plants by artificial inoculation with the isolates. This is the first report that S. caulophylli causes leaf and stem blight on columbine and bleeding heart in the world.